Scale of Structure Organization
X-Ray Diffraction (XRD) is used to study crystal structures. X-rays have wavelengths on the order of interatomic spacings (2-3 Angstroms), allowing them to diffract off of crystal planes. The diffraction patterns produced by crystals like quartz and cristobalite are different due to their differing atomic arrangements, despite being chemically identical. Amorphous materials like glass do not have long-range order and thus produce only broad scattering features in XRD.
To Study Principles of Microscopy: Light Microscope, Phase Contrast Microsco...Om Prakash
To Study Principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
ByOm Prakash
June 13, 2022
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on To Study Principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
Aim: To study principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
Table of Contents
THEORETICAL BACKGROUND:
Light Microscopy
History:
SIMPLE MICROSCOPE
Principles of Microscopy:
THE COMPOUND MICROSCOPE
Phase Contrast Microscope
Electron Microscopes
SCANNING ELECTRON MICROSCOPE (SEM)
Also Read
THEORETICAL BACKGROUND:
Light Microscopy
The light microscope is an instrument designed for the study of cells and tissues. It comprises of lenses that produce a magnified image of the object under study. The light microscope is considered to be a simple important invention that has contributed to the advancement of biological research.
History:
The ancient Greeks and Romans knew the use of Glass and quartz lenses. In the 14th century, spectacles and lenses were used to magnify objects. Galileo had constructed a microscope at the same time (1610). It was employed for the study of the arrangement of the compound eye of insects. Anton Von Leeuwenhoek (1674), the father of biology was the first to use the microscope for biological studies. His microscope has consisted of a single lens with a higher power of magnification. The compound microscope was constructed by Robert Hooke (1665) and is the forerunner of the present-day compound microscope.
SIMPLE MICROSCOPE
The simple microscope distinguishes between two points that are less than 0.1mm apart when placed at a normal viewing distance of 25cm. The two points appear as one and the eye fails to resolve or distinguish them as two distinct points. Another limitation of the human eye is that it cannot resolve any image less than 5µm.
A simple microscope consists of a single convex lens or a combination of lenses that functions as a convex lens. A convex lens magnifies the objects and also helps to produce a magnified image of a near object which appears to be at the distance of distinct vision.
The magnification obtained with a convex lens can be easily calculated by the formula
M = 25/f + 1
Where f= focal length, 25 is the distance of distinct vision in cm.
Principles of Microscopy:
1. Resolving power: It is defined as the capacity of the microscope to distinguish images of two pointed objects lying very close together. If two points are at a distance of more than 0.2 µm, they will appear as two points in the microscope.
2. Limit of resolution: It is defined as the minimum distance at which two objects appear as two distinct objects or entities. It can be calculated as:
Limit of Resolution: 0.61λ/NA = 0.61λ/n Sin θ
Where 0.61 is the constant representing the minimum detectable difference in contrast λ = wavelength of illumination
NA = Numerical aperture, light gathering capa
To Study Principles of Microscopy: Light Microscope, Phase Contrast Microsco...Om Prakash
To Study Principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
ByOm Prakash
June 13, 2022
Write a Comment
on To Study Principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
Aim: To study principles of Microscopy: Light Microscope, Phase Contrast Microscope & Electron Microscope
Table of Contents
THEORETICAL BACKGROUND:
Light Microscopy
History:
SIMPLE MICROSCOPE
Principles of Microscopy:
THE COMPOUND MICROSCOPE
Phase Contrast Microscope
Electron Microscopes
SCANNING ELECTRON MICROSCOPE (SEM)
Also Read
THEORETICAL BACKGROUND:
Light Microscopy
The light microscope is an instrument designed for the study of cells and tissues. It comprises of lenses that produce a magnified image of the object under study. The light microscope is considered to be a simple important invention that has contributed to the advancement of biological research.
History:
The ancient Greeks and Romans knew the use of Glass and quartz lenses. In the 14th century, spectacles and lenses were used to magnify objects. Galileo had constructed a microscope at the same time (1610). It was employed for the study of the arrangement of the compound eye of insects. Anton Von Leeuwenhoek (1674), the father of biology was the first to use the microscope for biological studies. His microscope has consisted of a single lens with a higher power of magnification. The compound microscope was constructed by Robert Hooke (1665) and is the forerunner of the present-day compound microscope.
SIMPLE MICROSCOPE
The simple microscope distinguishes between two points that are less than 0.1mm apart when placed at a normal viewing distance of 25cm. The two points appear as one and the eye fails to resolve or distinguish them as two distinct points. Another limitation of the human eye is that it cannot resolve any image less than 5µm.
A simple microscope consists of a single convex lens or a combination of lenses that functions as a convex lens. A convex lens magnifies the objects and also helps to produce a magnified image of a near object which appears to be at the distance of distinct vision.
The magnification obtained with a convex lens can be easily calculated by the formula
M = 25/f + 1
Where f= focal length, 25 is the distance of distinct vision in cm.
Principles of Microscopy:
1. Resolving power: It is defined as the capacity of the microscope to distinguish images of two pointed objects lying very close together. If two points are at a distance of more than 0.2 µm, they will appear as two points in the microscope.
2. Limit of resolution: It is defined as the minimum distance at which two objects appear as two distinct objects or entities. It can be calculated as:
Limit of Resolution: 0.61λ/NA = 0.61λ/n Sin θ
Where 0.61 is the constant representing the minimum detectable difference in contrast λ = wavelength of illumination
NA = Numerical aperture, light gathering capa
Introduction
The applications of microscopy in the forensic sciences are almost limitless. This is due in large measure to the ability of
microscopes to detect, resolve and image the smallest items of evidence, often without alteration or destruction. As a
result, microscopes have become nearly indispensable in all forensic disciplines involving the natural sciences. Thus, a
firearms examiner comparing a bullet, a trace evidence specialist identifying and comparing fibers, hairs, soils or dust, a
document examiner studying ink line crossings or paper fibers, and a serologist scrutinizing a bloodstain, all rely on
microscopes, in spite of the fact that each may use them in different ways and for different purposes.
The principal purpose of any microscope is to form an enlarged image of a small object. As the image is more greatly
magnified, the concern then becomes resolution; the ability to see increasingly fine details as the magnification is
increased. For most observers, the ability to see fine details of an item of evidence at a convenient magnification, is
sufficient. For many items, such as ink lines, bloodstains or bullets, no treatment is required and the evidence may
typically be studied directly under the appropriate microscope without any form of sample preparation. For other types of
evidence, particularly traces of particulate matter, sample preparation before the microscopical examination begins is
often essential. Types of Microscopes Used in the Forensic Sciences
A variety of microscopes are used in any modern forensic science laboratory. Most of these are light microscopes which
use photons to form images, but electron microscopes, particularly the scanning electron microscope (SEM), are finding
applications in larger, full service laboratories because of their wide range of magnification, high resolving power and
ability to perform elemental analyses when equipped with an energy or wavelength dispersive X-ray spectrometer.
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
Introduction
The applications of microscopy in the forensic sciences are almost limitless. This is due in large measure to the ability of
microscopes to detect, resolve and image the smallest items of evidence, often without alteration or destruction. As a
result, microscopes have become nearly indispensable in all forensic disciplines involving the natural sciences. Thus, a
firearms examiner comparing a bullet, a trace evidence specialist identifying and comparing fibers, hairs, soils or dust, a
document examiner studying ink line crossings or paper fibers, and a serologist scrutinizing a bloodstain, all rely on
microscopes, in spite of the fact that each may use them in different ways and for different purposes.
The principal purpose of any microscope is to form an enlarged image of a small object. As the image is more greatly
magnified, the concern then becomes resolution; the ability to see increasingly fine details as the magnification is
increased. For most observers, the ability to see fine details of an item of evidence at a convenient magnification, is
sufficient. For many items, such as ink lines, bloodstains or bullets, no treatment is required and the evidence may
typically be studied directly under the appropriate microscope without any form of sample preparation. For other types of
evidence, particularly traces of particulate matter, sample preparation before the microscopical examination begins is
often essential. Types of Microscopes Used in the Forensic Sciences
A variety of microscopes are used in any modern forensic science laboratory. Most of these are light microscopes which
use photons to form images, but electron microscopes, particularly the scanning electron microscope (SEM), are finding
applications in larger, full service laboratories because of their wide range of magnification, high resolving power and
ability to perform elemental analyses when equipped with an energy or wavelength dispersive X-ray spectrometer.
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
About
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Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
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Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface
• Compatible with MAFI CCR system
• Copatiable with IDM8000 CCR
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
Application
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
Explore the innovative world of trenchless pipe repair with our comprehensive guide, "The Benefits and Techniques of Trenchless Pipe Repair." This document delves into the modern methods of repairing underground pipes without the need for extensive excavation, highlighting the numerous advantages and the latest techniques used in the industry.
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Saudi Arabia stands as a titan in the global energy landscape, renowned for its abundant oil and gas resources. It's the largest exporter of petroleum and holds some of the world's most significant reserves. Let's delve into the top 10 oil and gas projects shaping Saudi Arabia's energy future in 2024.
Water scarcity is the lack of fresh water resources to meet the standard water demand. There are two type of water scarcity. One is physical. The other is economic water scarcity.
Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
CFD Simulation of By-pass Flow in a HRSG module by R&R Consult.pptxR&R Consult
CFD analysis is incredibly effective at solving mysteries and improving the performance of complex systems!
Here's a great example: At a large natural gas-fired power plant, where they use waste heat to generate steam and energy, they were puzzled that their boiler wasn't producing as much steam as expected.
R&R and Tetra Engineering Group Inc. were asked to solve the issue with reduced steam production.
An inspection had shown that a significant amount of hot flue gas was bypassing the boiler tubes, where the heat was supposed to be transferred.
R&R Consult conducted a CFD analysis, which revealed that 6.3% of the flue gas was bypassing the boiler tubes without transferring heat. The analysis also showed that the flue gas was instead being directed along the sides of the boiler and between the modules that were supposed to capture the heat. This was the cause of the reduced performance.
Based on our results, Tetra Engineering installed covering plates to reduce the bypass flow. This improved the boiler's performance and increased electricity production.
It is always satisfying when we can help solve complex challenges like this. Do your systems also need a check-up or optimization? Give us a call!
Work done in cooperation with James Malloy and David Moelling from Tetra Engineering.
More examples of our work https://www.r-r-consult.dk/en/cases-en/
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
Instead of buying and hoping for the best, we can use data science to help us predict which products may be good fits for us. It includes various function programs to do the above mentioned tasks.
Data file handling has been effectively used in the program.
The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
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3. Basics of Diffraction
For electromagnetic radiation to be diffracted the spacing in the grating
should be of the same order as the wavelength
In crystals the typical interatomic spacing ~ 2-3 Å so the suitable radiation
is X-rays
Hence, X-rays can be used for the study of crystal structures
Neutrons and Electrons are also used for diffraction studies from materials.
Neutron diffraction is especially useful for studying the magnetic ordering
in materials
4.
5.
6. The three X-ray scattering patterns above were produced by three chemically
identical forms SiO2
Crystalline materials like quartz and Cristobalite produce X-ray diffraction patterns
– Quartz and Cristobalite have two different crystal structures
–The Si and O atoms are arranged differently, but both have long-range atomic
order
–The difference in their crystal structure is reflected in their different diffraction
patterns
The amorphous glass does not have long-range atomic order and therefore
produces only broad scattering features
7.
8.
9. Optical Microscope
Optical microscope is a type of microscope which
uses visible light and a system of lenses to magnify
images of small samples.
There are two basic configurations of the conventional
optical microscope in use, the simple (one lens) and
the compound (many lenses).
10. Assumptions in Light Theory
1.Light travels in a straight line.
2.Portions of light beams can be treated as
individual rays.
3.Law of reflection.
4.Law of refraction.
12. Optics of a Microscope
Slide with the letter “F”
The letter “F” as it
appears when
viewed through the
eyepiece
F
Images viewed through the
eyepiece of compound
microscopes will appear
upside-down and
backwards.
13. How it Works?
Light
source or
Mirror
Optical
Condenser
Light
passes
through the
specimen
Objective
Lenses
(X100)
First Image
Eyepiece
or Ocular
(X10)
Image
(X1000)
Retina
14. Basic Components
Eyepiece Lens: the lens at the
top that you look through.
Tube: Connects the eyepiece to
the objective lenses
Objective Lenses: Usually you
will find 3 or 4 objective lenses
on a microscope. They almost
always consist of 5X, 10X, 20X ,
50X and 100X powers.
Rack Stop: This is an
adjustment that determines how
close the objective lens can get
to the slide.
Condenser Lens: The purpose
of the condenser lens is to focus
the light onto the specimen.
15. Basic Components
Stage: The flat platform where you
place your slides. Stage clips hold
the slides in place.
Revolving Nosepiece or Turret:
This is the part that holds two or
more objective lenses and can be
rotated to easily change power.
Illuminator: A steady light source
(110 volts) used in place of a mirror.
If your microscope has a mirror, it is
used to reflect light from an external
light source.
Arm: Supports the tube and
connects it to the base
Base: The bottom of the
microscope, used for support
16. Total Magnification
• The total magnification of
the specimen being viewed
is calculated using the
ocular lens multiplied by
the objective lens.
• For example, if the ocular
lens is 10x and the ocular
lens is 50x then the total
magnification would be
500x.
18. Focusing a Microscope
• Course-adjustment knob- is the
larger of the two knobs. It is used
in bringing the object into quick
focus.
• Fine-adjustment knob- is used for
improving the clarity of the image,
especially when viewing under
high power.
19. Quality of an Image
• Focal Length
• Size of sample
• Type of sample
• Quality of Microscope and lenses
• Amount of light on the sample
• Quality of sample
20. Numerical Aperture (NA)
The objective collects as much light as possible coming from any
point on the specimen and combines this light to form the image.
The numerical aperture (NA) is a measure of the light collection
capability of the objective and is defined as 𝑵𝑨 = 𝒏. 𝐬𝐢𝐧 𝜶
Objectives with a shorter focal length have a wider angular aperture and therefore
deliver a higher NA and resolution.
21. Numerical Aperture (NA)
The objective with the higher numerical aperture is
clearly able to give us more detail with separation
between the wavelets.
https://microscopeclarity.com/what-is-microscope-resolution/
22. Depth of Field
• The depth of field is the
distance along the optical
axis over which details of
the object can be observed
with adequate sharpness
• "Depth of field" refers to the
thickness of the plane of
focus.
• It is the vertical distance
(from above to below the
focal plane) that yields a
useful image.
23. The series of images show
how the depth of field can
influence the appearance
of an image.
With narrow
depth of
field, only
part of the
image is in
focus at the
same time.
With a large
depth of field,
the entire
image is in
focus at the
same time.
Depth of Field: Example
24. Resolution
• Resolution or resolving power is the closest spacing
of two points which can clearly be seen through the
microscope to be separate entities.
• It is not necessarily the same as the smallest point
which can be seen with the microscope, which will
often be smaller than the resolution limit.
• Resolving power, 𝑑 =
0.61𝜆
𝑛.sin 𝛼
=
0.61𝜆
𝑁𝐴
26. • Inexpensive
• Easy to learn and operate
• Very sharp plane of focus
• Small and portable
Advantages
• Magnification is limited
• Specimen may be disfigured during preparation to be
viewed under the microscope.
• Only has a resolution of 0.2 μm - which is relatively poor
in comparison to other microscopes.
• Poor surface view
Disadvantages
27. Allotriomorphic ferrite in a Fe-0.4C
steel which is slowly cooled; the
remaining dark-etching microstructure
is fine pearlite
Optical micrograph showing colonies of
pearlite (courtesy S. S. Babu)
https://www.phase-trans.msm.cam.ac.uk/2008/Steel_Microstructure/SM.html
Microstructure: Examples
28. Left, as-cast cadmium etched with 2% nital, 30 x ; right, as-cast alloy of Cd and 10% Bi etched
with 2% nital, 60 x .
Microstructure: Examples
29. Microstructure: Examples
Superpure aluminum anodized using the method of Hone and Pearson (Ref. 56),
polarized light, 32 x . Left, held 6 min at 375°C, partly recrystallized. Right, held 2 h at
375°C, fully recrystallized. (Courtesy of E. C. Pearson, Alcan International Ltd.)
30. Color etching of various grain or mixed-crystal areas and sulphate layers
of different thicknesses
Ferrite-perlite microstructure, the ferrite
is tinted while Fe3C is kept white
Klemm (K) etch
This contrasting visualizes the quality
of a soft annealing (K)
https://www.leica-microsystems.com/science-lab/metallography-with-color-and-contrast/
Microstructure: Examples
31. Brightfield image of a cast austenite
structure caused by a laser melting
process
Improved contrast using interference
Microstructure: Examples
The same sample in interference contrast
showing clear contrasting of the
dendrites (B)
Klemm (K) etchant: 50 mL saturated aqueous sodium thiosulfate, 1 g potassium metabisulfites
Beraha (B) etchant: (a) Stock solution: 1:2, 1:1, or 1:0.5 HCI-water
(b) 100 mL stock solution plus 0.6-1.0 g potassium metabisulfite
https://www.leica-microsystems.com/science-lab/metallography-with-color-and-contrast/
34. A brief description of each system follows:
1. Vacuum system. A vacuum is required when using an electron beam because
electrons will quickly disperse or scatter due to collisions with other molecules.
2. Electron beam generation system. This system is found at the top of the
microscope column. This system generates the "illuminating" beam of electrons known
as the primary electron beam.
3. Electron beam manipulation system. This system consists of electromagnetic
lenses and coils located in the microscope column and control the size, shape, and
position of the electron beam on the specimen surface.
4. Beam specimen interaction system. This system involves the interaction of the
electron beam with the specimen and the types of signals that can be detected.
5. Detection system. This system can consist of several different detectors, each
sensitive to different energy / particle emissions that occur on the sample.
6. Signal processing system. This system is an electronic system that processes the
signal generated by the detection system and allows additional electronic manipulation
of the image.
7. Display and recording system. This system allows visualization of an electronic
signal using a cathode ray tube and permits recording of the results using photographic
or magnetic media.
Components of SEM
35. Principle of SEM image formation
When an electron beam is incident
on the sample then many different
types of signals are generated which
are eventually used to observe or
analyze morphology/ topology of the
sample. SEM is also used for
elemental and state analysis. These
signals includes – Secondary
electrons, Backscattered electrons,
Auger electrons,
Cathodoluminescence and X-rays.
Interaction of Electrons with Specimen: Electrons entering specimen gets
scattered within it and lose their energy gradually upon getting absorbed
within the sample. Scattering range within specimen depends upon-
Energy of electrons – More Energy More Scattering.
Element’s atomic number (Z) making the sample - More Z Less
Scattering.
Density of constituent atoms – More Density less scattering.
36. SEM Sample-
Conducting samples provide a path to ground for the beam electrons, and therefore
require no special preparation. Insulating materials, however, require a thin coating
of a conductor (often carbon or gold) in order to prevent charging.
Sample Preparation-
It is done in order to eliminate the sample charging few steps are followed:
1. Charging: A thin metal coating of about 10nm is done on the sample because
metal film is highly stable and its secondary electron yield is higher. Too thin coating
is not preferred because continuity is lost.
2. Low accelerating voltage: Low KV value of about 1KV can be used to scan
insulating samples because the number of incident electrons becomes equal to the
number of emitted secondary electrons, implying that the sample is not charged.
3. Tilt Observation: In this case secondary electrons yield is higher as electron
beam is entering at an angle.
4. Low Vacuum SEM observation: Ondecreasing the vacuum, the gas molecules
within the sample chamberincreases, which get ionized due to electrons and thus,
on reaching the specimen as positive ions neutralize the charging.
SEM sample Preparation
39. Construction of TEM Instrument components
Electron gun (described
previously)
Condenser system (lenses &
apertures for controlling
illumination on specimen)
Specimen chamber
assembly
Objective lens system
(image-forming lens - limits
resolution; aperture -
controls imaging conditions)
Projector lens system
(magnifies image or
diffraction pattern onto final
screen)