jnkvv college of agriculture tikamgar

2. MASS REARING TECHNIQUES OF
HELICOVERPA ARMIGERA
Guided to
Dr.M.K.Nayak
Presented by
Kavita bisen
Roll No.-5765
DEPARTMENT OF ENTOMOLOGY
KRISHI MAHA VIDYALAYA, TIKAMGARH, (M. P.)

3. MASS REARING OF HELICOVERPA
armigera
COMMON NAME - GRAM POD BORER
S.N. - Helicoverpa armigera
ORDER - Lepidoptera
FAMILY – Noctuidae
BIOLOGY -
Biology of the gram pod borer reared on
Bengal gram and on artificial diet is given in table

4. Life stage Bengal gram
natural food
Artificial diet
Larval period (day) 25 22.10
Pupal period (day) 11 14.10
Male (day) 6 7.8
Female (day) 10 9
Fecundity
(egg/family)
300 130
Incubation
period(day)
4.5 4.9
Viability of egg(%) 90 86

5. Equipment and facilities
Food material (viz.-macerated paste of soaked bengal
gram seeds), distilled water, glass tube(10×2.5 cm.),
jars(20×15 cm.), cotton wool, markin cloth, smooth hair
brush, autoclave and a room fitted with all amentias for
rearing the insect as explained here.
REARING
freshly hatched larvae of pod borer may be fed on
macerated pest of soaked bengal gram seeds for 2-3 days
later, they are separated and reared in individually tubes
(10×2.5 cm.) till population takes place by placing 2-8
grains of soaked bengal gram seeds.

6. everyday normally , population takes place inside the
cotton plug used for securing the host inside the
tube. pupae are sexed and kept separately. The
techniques as also used for other Lepidopteron pupae
the emerged adults are kept in our of (20×15 cm.) for
oviposition and a cotton scab clipped in sugar
solution (10%) is hung in the jars . larvae can be
reared on artificial diet by placing 8 gm. Cake for each
individual larvae into a tube to avoid cannibolism.
it should be reared at 25±2⁰C and 60±5% RH.

7. Precaution
1. Rear second instar larvae to individual
tubes to avoid cannibolism
2. Do not case more than 5 pairs per jar for
good oviposition.
3. Starts next generation from the first batch
oviposition reared from healthy adults.

8. 4. Always sterilized the diet and cotton plugs
in an autoclave.
5. First treat eggs with sodium by chloride
(6.157%) and then with Na thiosulphate
(10%) to separate them from the
substratum. Which also acts as surface
strilant.