MASS PRODUCTION OF
BIOAGENTS
&
BIOPESTICIDES
Balram Solunke
balram2424@gmail.co
 CONTENT:
 Mass production of....
 Trichoderma viride
 Corcyra cephalonica
 cryptolaemus montrouzieri
 Trichogramma ...
Mass production of
Trichoderma viride
Materials :
1)Potato
2)Dextrose
3)Glass wares
4)Spirit lamp
5)Pure culture of fungus...
Inoculation:
MIXING OF CULTURE
mix well with talcum powder for final product of
Trichoderma viride
mix well with talcum powder for final product of
Trichoderma viride
Packing:
Prepare POB broth media
Cooking of 200gm potato for 1 lit media than
extract taken & add water &mix 20gm dextrose
Than mak...
Fill media in 1 lit conical flask & plugged
with aluminum foil than tied with rubber
band
Sterilize media & requiered glas...
Sterile LAF with ethyl alcohol than transfer
glass wares & media in LAF
Pour media in bottle , Petridis
Inoculate pure cul...
Collect the fungus growth & crush these
culture with the help of blander mix these
with the help of blander
After that mix...
Mix CMC (carbon methyl cellulose) as
sticky agent in dried powdery form
Packing product in polythene bags &
labeling
Mass production of
Corcyra cephalonica
Materials :
1) One rearing room of 15x12x8 cm size with
a.c installation
2) Wooden ...
Procedure sorghum / bajra
Grains should be milled coarsely to make 3-4
pieces of each grains
Heat/sterilized in an oven at...
Spray 0.1% formalin solution uniformly & mix well
Dry the grain with fan air
Pour 5 kg coarsely ground grains of jawar or ...
Obtain pure culture of corcyra cephalonica
Mix 1cc 18000 to 20000 egg in each box
Cover box for 30 days approximately
Maintain temperature of rearing room at 300c
After 20-40 days on alternate day collect adults
Put 1000 adults oviposition ...
Collect the deposited eggs, clean from scales &
debris
Store for 3-4 days in refrigerator at 100c it
requiered,these eggs ...
Mass production of
cryptolaemus montrouzieri
a predator on mealy bug
REARING OF MEALY BUG ON
POTATO :
Fill wooden/plastic trays with sandy silt soil up to
2 to 3 cm deapth for planting
Place 10-15 potato tubers & cover with ...
Watering such trays
Put ovisacks of mealy bugs over potato sprouts
With in 20-25 days mass culture of mealy bugs
could be ...
REARING OF MEALY BUG ON
RED PUMPKINS :
1) REARING OF MEALY
BUG ON RED PUMPKINS :
Medium sized with ridges & furrows & grooves
with small stack & pumpkin should b...
The wound if any pumpkin plug with paraffin wax
Than tied the whole pumpkine with thread for
griftting the mealy bug on it...
Collected mealy bug release on pumpkin
Cages placed on working tables/racks in tearing
room
Keep the pumpkin in wooden cage
1) REARING OF
PREDATOR
cryptolaemus montrouzieri :
When mealy bugs are 8-10 days old a stock of
15-20females of cryptolaem...
On hatching larvae feed on mealy bug
Female deposit eggs on pumpkins
Put fully developed larvae with mealy bugs on as
food...
REARING OF PREDATOR
cryptolaemus montrouzieri
MASS PRODUCTION
TECHNIQUES OF
TRICHOGRAMMA CHILONIS
Procure eggs of corcyra cephalonica
Paste 1cc (18000 to 20000) egg on thinly
smeared gum arabic on egg card with the help ...
Transfer 6 days old daily parasitoid egg card with
1:6 ratio (parasite : host) for 24 hrs.
After 4 days of parasitization ...
PREPARATION OF TRICHOCARD
:
MASS PRODUCTION
TECHNIQUES OF
Zygogramma bicolarata
Take parthanium leaves in transparent plastic
container
Release 10 pairs of beetles female& male on
such leaves
Egg laying...
these process repeated for month
After few days eggs collect in plastic small bottle &
kept leaves of parthanium in bottle...
15-20 small plants transplanted in aluminum cage
these cages yield about 100-125 adults
MASS PRODUCTION
TECHNIQUES OF NUCLEAR
POLYHYDROSIS VIRUS OF
HELICOVERPA ARMIGERA
FIELD COLLECTION OF
HELICOVERPA ARMIGERA FROM TUR
FIELD :
PREPARATION OF ARTIFICIAL
DIET :
MATING OF HELICOVERPA
ARMIGERA MOTH IN GLASS
CHMBER :
PUPATION BOX :
Select late third instar or early 4th instar larvae of
HELICOVERPA ARMIGERA for virus inoculation
Pre- strave these larvae...
The larvae begin to show symptoms of virus
infections after about 4-5 days
Collect the dead diseased larvae in distilled w...
Pullet staved at the bottom may be discard &
centrifuge at 2500 rpm for 10 min
Supernatant is discard & pellet mix in wate...
MASS PRODUCTION
TECHNIQUES OF NUCLEAR
POLYHYDROSIS VIRUS OF
SPODOPTERA LITURA
Collection of SPODOPTERA LITURA from cabbage field :
Prepare NPV suspension in water for leaf
treatment
Pluck castor leaves with the petiole & dip in the
virus solution
Dry tr...
Keep the news paper at the bottom of bucket &
provide the trated leaf for feeding cover mouth of
bucket with cloth
Everyda...
Larvae will start dying after 4-5 days
Collect the dead larvae in distilled water & grind
the larvae thoroughly stir the s...
Thank You
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
Mass production of bio pesticides and bio agents.
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Mass production of bio pesticides and bio agents.

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Detail Mass production of....
Trichoderma viride
Corcyra cephalonica
cryptolaemus montrouzieri
Trichogramma chilonis
Zygogramma bicolarata
Nuclear polyhydrosis virus of Helicoverpa armigera
Nuclear polyhydrosis virus of Spodoptera litura.
in this ppt you will get all detail mass production procedure of all mentioned above bio pesticides and bio agents.

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Mass production of bio pesticides and bio agents.

  1. 1. MASS PRODUCTION OF BIOAGENTS & BIOPESTICIDES Balram Solunke balram2424@gmail.co
  2. 2.  CONTENT:  Mass production of....  Trichoderma viride  Corcyra cephalonica  cryptolaemus montrouzieri  Trichogramma chilonis  Zygogramma bicolarata  Nuclear polyhydrosis virus of Helicoverpa armigera  Nuclear polyhydrosis virus of Spodoptera litura
  3. 3. Mass production of Trichoderma viride Materials : 1)Potato 2)Dextrose 3)Glass wares 4)Spirit lamp 5)Pure culture of fungus 6)Laminar air flow 7)Ethyl alcohol 8)Cotton plug, rubber band,alluminium foil etc.
  4. 4. Inoculation:
  5. 5. MIXING OF CULTURE
  6. 6. mix well with talcum powder for final product of Trichoderma viride
  7. 7. mix well with talcum powder for final product of Trichoderma viride
  8. 8. Packing:
  9. 9. Prepare POB broth media Cooking of 200gm potato for 1 lit media than extract taken & add water &mix 20gm dextrose Than make final volume 1 lit Procedure
  10. 10. Fill media in 1 lit conical flask & plugged with aluminum foil than tied with rubber band Sterilize media & requiered glasswares like test tube petriplates & inoculation bottles for 15 min in 1210c in autoclave Kept these for cooling
  11. 11. Sterile LAF with ethyl alcohol than transfer glass wares & media in LAF Pour media in bottle , Petridis Inoculate pure culture of trchoderma viridae with the help of inoculating needle using spirit lamp to avoid contamination
  12. 12. Collect the fungus growth & crush these culture with the help of blander mix these with the help of blander After that mix well with talcum powder for final product of Trichoderma viridae Drying of product at room temp for 1 day
  13. 13. Mix CMC (carbon methyl cellulose) as sticky agent in dried powdery form Packing product in polythene bags & labeling
  14. 14. Mass production of Corcyra cephalonica Materials : 1) One rearing room of 15x12x8 cm size with a.c installation 2) Wooden tray of 45x30x15 cm size 3) Coarsely ground grains of sorghum/bajra 4) Yeast 5) Eggs of corcera
  15. 15. Procedure sorghum / bajra Grains should be milled coarsely to make 3-4 pieces of each grains Heat/sterilized in an oven at 1000 c for 30 min
  16. 16. Spray 0.1% formalin solution uniformly & mix well Dry the grain with fan air Pour 5 kg coarsely ground grains of jawar or bajra in each wooden box & add 10gm yeast powder + 200gm g.nut
  17. 17. Obtain pure culture of corcyra cephalonica Mix 1cc 18000 to 20000 egg in each box Cover box for 30 days approximately
  18. 18. Maintain temperature of rearing room at 300c After 20-40 days on alternate day collect adults Put 1000 adults oviposition cage
  19. 19. Collect the deposited eggs, clean from scales & debris Store for 3-4 days in refrigerator at 100c it requiered,these eggs treat with UV rays for 45 min.
  20. 20. Mass production of cryptolaemus montrouzieri a predator on mealy bug
  21. 21. REARING OF MEALY BUG ON POTATO :
  22. 22. Fill wooden/plastic trays with sandy silt soil up to 2 to 3 cm deapth for planting Place 10-15 potato tubers & cover with moist soil Fill such tray frequently 1) REARING OF MEALY BUG ON POTATO :
  23. 23. Watering such trays Put ovisacks of mealy bugs over potato sprouts With in 20-25 days mass culture of mealy bugs could be obtained such trays Maintain the temperature in rearing room between 20 to 300c
  24. 24. REARING OF MEALY BUG ON RED PUMPKINS :
  25. 25. 1) REARING OF MEALY BUG ON RED PUMPKINS : Medium sized with ridges & furrows & grooves with small stack & pumpkin should be selected for the mealy bug rearing Clean with water to remove dust particles
  26. 26. The wound if any pumpkin plug with paraffin wax Than tied the whole pumpkine with thread for griftting the mealy bug on it Treat 0.1% bavistine solution (1gm/lit) to prevent it’s rotting during rearing process
  27. 27. Collected mealy bug release on pumpkin Cages placed on working tables/racks in tearing room Keep the pumpkin in wooden cage
  28. 28. 1) REARING OF PREDATOR cryptolaemus montrouzieri : When mealy bugs are 8-10 days old a stock of 15-20females of cryptolaemus montrouzieri at temperature of 20-300c The released female feed on mealy bugs eggs , numph & adults
  29. 29. On hatching larvae feed on mealy bug Female deposit eggs on pumpkins Put fully developed larvae with mealy bugs on as food plastic jar & provide paper strips for its pupation The emerging beetles collect in plastic bowl & feed with honey agar or mealy bugs
  30. 30. REARING OF PREDATOR cryptolaemus montrouzieri
  31. 31. MASS PRODUCTION TECHNIQUES OF TRICHOGRAMMA CHILONIS
  32. 32. Procure eggs of corcyra cephalonica Paste 1cc (18000 to 20000) egg on thinly smeared gum arabic on egg card with the help of suitable strain Expose the eggs to UV rays for 45 min On backside of the trichocards written down the mentioned information
  33. 33. Transfer 6 days old daily parasitoid egg card with 1:6 ratio (parasite : host) for 24 hrs. After 4 days of parasitization brush out host larvae of day hatched Such parasitized eggs cards could be stored for about 20 to 30 days at 100c temperature in refrigerator
  34. 34. PREPARATION OF TRICHOCARD :
  35. 35. MASS PRODUCTION TECHNIQUES OF Zygogramma bicolarata
  36. 36. Take parthanium leaves in transparent plastic container Release 10 pairs of beetles female& male on such leaves Egg laying observed in leaves replaced & fresh bouquets are provided
  37. 37. these process repeated for month After few days eggs collect in plastic small bottle & kept leaves of parthanium in bottle Emergence of larvae & feed on leaves & pupa in soil
  38. 38. 15-20 small plants transplanted in aluminum cage these cages yield about 100-125 adults
  39. 39. MASS PRODUCTION TECHNIQUES OF NUCLEAR POLYHYDROSIS VIRUS OF HELICOVERPA ARMIGERA
  40. 40. FIELD COLLECTION OF HELICOVERPA ARMIGERA FROM TUR FIELD :
  41. 41. PREPARATION OF ARTIFICIAL DIET :
  42. 42. MATING OF HELICOVERPA ARMIGERA MOTH IN GLASS CHMBER :
  43. 43. PUPATION BOX :
  44. 44. Select late third instar or early 4th instar larvae of HELICOVERPA ARMIGERA for virus inoculation Pre- strave these larvae for 6-8 hrs. before virus inoculation Introduce piece of the virus contaminated diet in each container with pre-starved larvae
  45. 45. The larvae begin to show symptoms of virus infections after about 4-5 days Collect the dead diseased larvae in distilled water Replace uneaten part of diet after 2-3 days & clean the containers
  46. 46. Pullet staved at the bottom may be discard & centrifuge at 2500 rpm for 10 min Supernatant is discard & pellet mix in water 10 times in purified virus Filter this suspension through double layers of muslin cloth & centrifuge for 3 to 5 min at 500 rpm
  47. 47. MASS PRODUCTION TECHNIQUES OF NUCLEAR POLYHYDROSIS VIRUS OF SPODOPTERA LITURA
  48. 48. Collection of SPODOPTERA LITURA from cabbage field :
  49. 49. Prepare NPV suspension in water for leaf treatment Pluck castor leaves with the petiole & dip in the virus solution Dry treated leaf under shade Starve larvae for 4 to 5 hrs before feeding
  50. 50. Keep the news paper at the bottom of bucket & provide the trated leaf for feeding cover mouth of bucket with cloth Everyday remove the excreta of larvae Provide untrated leaf for feeding
  51. 51. Larvae will start dying after 4-5 days Collect the dead larvae in distilled water & grind the larvae thoroughly stir the solution & filter it by cloth Collect filtrate store in amber coloured bottles in colol place
  52. 52. Thank You

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