1. Loose Egg Production- Steps for seed cocoon production and handling:
i) Selection of seed farmers
ii) Preparation for rearing, disinfection of rearing house
iii) Supply of incubated P1 seeds
iv) Rearing
v) Harvesting of seed cocoons
2. STAGE-I: DAY-WISE EXPLANATION
DAY 1
~ Seed Cocoon receipt & Processing
~ Quality inspection of seed cocoons as per norms
DAY 2 & 3
~ Cocoon cutting
~ Pupal sex separation
~ Preservation of pupae
3. STAGE II: Moth Emergence
( 4th- 7th day at grainage)
4. CARE TO BE TAKEN IN THE POST OVIPOSITION ACTIVITIES
5. ACID TREATMENT & AFTERWARD
6. FLOW CHART OF HYBERNATED EGGS.
Presented by: Jessy Daniel, Scientist- D, CSB
Prepared by: Silga de Sankey, Student
Data: Various study reports
1. B I V O L T I N E
H Y B R I D
Jessy Daniel, Scientist- D
Central
Silk
Board
E G G P R O D U C T I O N
2.
3. Technology support to seed farmers
Seed cocoons receipt and processing
Pupal sex separation
Moth emergence
Pebrine examination
Loose eggs handling
Incubation and disposal
Maintenance of grainage premises and equipment
4. SEED COCOON RECEIPT & PROCESSING
1st day at grainage after seed harvesting
I
2
1
Purchase ofseed
cocoons fromP1 seed
farmers atgrainage
Next day after harvesting
based on pupation rate
Quality
inspection of
seed cocoons
Assessment of 1/2 kg
cocoons as per
prescribed norms
5. COCOON ASSESSMENT
Norms
1 2 3 4 5
DISEASE
FREENESS
Free from
pebrine
and
muscardine
PUPATION
RATE
PURITY YIELD PER
100 DFLS
COCOONS
PER KG
More
than 80%
No
mixing of
race
50 kg
per 100
DFLs
Number
between
550-700
6. 1st day at grainage
After inspection
3
Spreading of
seed cocoons
In a single layer in
60 cm X 90 cm
plastic tray
Deflossing for
cocoon
cutting
Deflossing for
cocoon cutting
4
Cocoon
Sorting
To ensure true
hybrid
preparation
5
7. 2nd & 3rd day at grainage
Cocoon
cutting
Manual or
mechanical
Visual observation of
pupae and seperation
by the skilled
of sex
persons
Pupal sex
seperation
Preservation of
pupae
Using corrugated
sheets
25 + / - 1°C & 85 + / - 5%
L: D = 10 h : 14 h
8. CHARACTER MALE
FEMALE
1.S kin colour
2.S ex
marking
3.Posterior
end
Difference between female and male pupa
Light
Ventral side of 8th
abdominal segment
bears X mark
Round
Dark
Ventral side of 9 th
abdominal segment
bears small brown spot
Pointed
9. How to
separate
the male
and female
pupae?
Separate the male & female pupae by visual
observation in bright light.
Do not sex separate the pupae based on size.
Only by visual observation of sexual marking on
the 8th and 9th abdominal segment seperate male
and female pupae.
Sex seperation must be done by trained, skilled
young technical staff whose eyesight is sharp.
Check the female pupae twice and ensure that
there are no male pupae in it.
10. Preservation
ofpupae 600-650 female pupae and 650-700 male
pupae can be spread in each tray.
Cover the pupae with holed newspapers/
holed corrugated sheets.
Keep 5-10 empty cocoon shells of the
particular race in trays for easy
identification.
Spreading of pupae on corrugated sheet
Place the corrugated sheets on plastic
trays (60 cm x 90 cm), and spread the
pupae over it.
11. MOTH EMERGENCE
II
5:00
AM
L ig h t o n f o r
m o t h
e m e r g e n c e 6:00
AM
Col l ection of m o t h s
Use moth picking nets or
holed newspapers/
corrugated sheets
7:30
AM
Pairing
Chinese x Japanese
and JxC; Reject
selfing, if any
10:30
AM
Depairing
2.5 to 3 hours
after pairing
8:00
AM
Singles
Pic k ing
After 30-45
minutes of pairing
12. 4 th to 7 th day at grainage
Male m o t h
preservation
Different race in
different colour
trays
(7-9° C)
K e e p i n g
females for
egg laying
225-250 in each
sheet of size
65 cm X 95 cm
Egg laying
Conditions:
Temp. 25 +/- 1° C
Humidity 85 %
RH
Total darkness
13. Critical areas Check point Norms
Handling of
loose eggs
Hydrochloric
acid treatment
Washing of loose eggs
1.Quality of acid
2.Procedure of acid
treatment
Water temperature between 20 and
26° C
Concentration of bleaching powder
0.4%
Quantity of eggs in each bag (600-
750 g)
Chemically pure (acid)
Time, duration, temperature of
treatment
Specific gravity of acid
Removal of acid traces and water
temperature between 20 & 26 ° C.
Post-Oviposition Activities
Incidence
of diseases
Identification of pebrine
spores
Mother moth sampling method
Counter checking by second
person
14. Critical areas Check point Norms
Incubation of
F1 seed
Method
Usage of incubation frames
Temperature 25° C
Humidity 80 to 85 %
Photoperiod 16 L :8 D
Transportation in cooler hours.
Post-Oviposition Activities
Hibernation Schedule Aestivation period
Intermediate temperature
Preservation in the cold storage
Humidity in the cold storage
16. FLOW CHART FOR HIBERNATED EGGS
Keep egg sheets
at 25 + / - 1° C
Follow estivation
period as per
schedule
TO INCUBATION
Remove hatched larvae,
if any
Pre- soaking of egg
sheets in water
Collection of loose eggs
Degumming & surface
disinfection
Drying
Preserve under
Hibernation
schedule for 4,6
or 10 months
Release the
eggs after the
schedule
duration
