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““ Trichoderma SpeciesTrichoderma Species ””
DEPARTMENT OF DESEASE
JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA,
JABALPUR, (M. P.)
Guided by : Presented by:
Dr. D.S.Tomar Devendra Rajpoot
Roll No. 5756
3
INTRODUCTION
• Trichoderma is taken from thix-hair , derma-skin and the
genus was introduced by persoon.1794
• Later fries and koning 1902.-made first report from soil
and first author named the type species Trichoderma
koningii oud in the honour of junior author .
• Sometimes of use for controlling pathogenic fungi .
• Trichoderma species can readily be isolated from soil either
through soil plate or soil dilution plate methods .
• It can be grown on number of media.
• Agar media is more effective and well suited to laboratery
• Water and PDA are some of the media for cultivation and
maintenance of culltures.
4
- Taxonomix description of trichoderma species
- Trichoderma a hamatum .-
- Colonies; - slow growing.
- Chlamydospores=mostly globose to sub globose,smooth,7-12 um
in diam.
-Conidiophores= Highly remified from very compact tufy ,2-3 barrel
shaped cell.
- Phialides= Smooth , hyaline to pale greenish, short plump , pear
shaped oviod.
- Phialospores = Aggregate into slime , globose , conical , pale green ,
smooth .
- Trichoderma harzianum Rifai.
-Colonies= Growing rapidly , white green to bright green to dull
green .
- Mycelium ;- Septate ,colourlees , smooth ,1.5-2.5 um .
- Chlamydospores = mostly globose , smooth , 6-12 um in diam .
- Conidiophores = Loose tufy , main branch produced numerous side
branches specially lower portion .
-
(Linnaeus 1758)
5
- Phialides ;= Arise flase varticellate upto five , short , skittle
shapes narrow at the base .
- Phialospores = subglobose , short obovoid , smooth , pale
green much darker in mass,2.8-2.5 um .
- Trichoderma koningii Oudem ;-
- Colonies = fast growing , greenish white to dull to dark
green .
- Mycelium = hyaline , highly ramified ,2-5 um.
- Chlamydospores = formed in submerged hyphae , globose
upto 12 um in diam .
- Conidiophores = much branched , compact or loose tufy ,in
group of 2-3 at wide angles .
- Phialides = pin shaped ,7-2.5 um .
- Phialospores =Elliptical ,oblong ,pale green ,3-2 um size .
- Trichoderma piluliferum Rifai ;-
- Colonies = slow growing , white mycelial over the surface .
6
- Mycelium = smooth , colourless , upto 10um in diam .
- Chlamydospores = highly ramified ,compact tufy , main
branches 5-7um diam ,thick .
- Phialides = typically short , plump , 4-6um ,short flask
shaped .
- Phialospores = Produced singly and accumulate at the
tip, globose, to sub globose ,smooth ,colourless, 2-3um
size .
- Trichoderma polysporum ;-
- Colonies = slow growing, watery white .
- Mycelium = hylaline , smooth 2-3um .
- Conidiophores = Irregular , close together, tufy long,
- Phialides = short , plump , pear shaped ,conicol neck ,.
- Phialospores = smooth , 2.8-1.8um .
-
7
Trichoderma Species Identification and Taxonomy

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Trichoderma Species Identification and Taxonomy

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  • 2. 2 ““ Trichoderma SpeciesTrichoderma Species ”” DEPARTMENT OF DESEASE JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA, JABALPUR, (M. P.) Guided by : Presented by: Dr. D.S.Tomar Devendra Rajpoot Roll No. 5756
  • 3. 3 INTRODUCTION • Trichoderma is taken from thix-hair , derma-skin and the genus was introduced by persoon.1794 • Later fries and koning 1902.-made first report from soil and first author named the type species Trichoderma koningii oud in the honour of junior author . • Sometimes of use for controlling pathogenic fungi . • Trichoderma species can readily be isolated from soil either through soil plate or soil dilution plate methods . • It can be grown on number of media. • Agar media is more effective and well suited to laboratery • Water and PDA are some of the media for cultivation and maintenance of culltures.
  • 4. 4 - Taxonomix description of trichoderma species - Trichoderma a hamatum .- - Colonies; - slow growing. - Chlamydospores=mostly globose to sub globose,smooth,7-12 um in diam. -Conidiophores= Highly remified from very compact tufy ,2-3 barrel shaped cell. - Phialides= Smooth , hyaline to pale greenish, short plump , pear shaped oviod. - Phialospores = Aggregate into slime , globose , conical , pale green , smooth . - Trichoderma harzianum Rifai. -Colonies= Growing rapidly , white green to bright green to dull green . - Mycelium ;- Septate ,colourlees , smooth ,1.5-2.5 um . - Chlamydospores = mostly globose , smooth , 6-12 um in diam . - Conidiophores = Loose tufy , main branch produced numerous side branches specially lower portion . - (Linnaeus 1758)
  • 5. 5 - Phialides ;= Arise flase varticellate upto five , short , skittle shapes narrow at the base . - Phialospores = subglobose , short obovoid , smooth , pale green much darker in mass,2.8-2.5 um . - Trichoderma koningii Oudem ;- - Colonies = fast growing , greenish white to dull to dark green . - Mycelium = hyaline , highly ramified ,2-5 um. - Chlamydospores = formed in submerged hyphae , globose upto 12 um in diam . - Conidiophores = much branched , compact or loose tufy ,in group of 2-3 at wide angles . - Phialides = pin shaped ,7-2.5 um . - Phialospores =Elliptical ,oblong ,pale green ,3-2 um size . - Trichoderma piluliferum Rifai ;- - Colonies = slow growing , white mycelial over the surface .
  • 6. 6 - Mycelium = smooth , colourless , upto 10um in diam . - Chlamydospores = highly ramified ,compact tufy , main branches 5-7um diam ,thick . - Phialides = typically short , plump , 4-6um ,short flask shaped . - Phialospores = Produced singly and accumulate at the tip, globose, to sub globose ,smooth ,colourless, 2-3um size . - Trichoderma polysporum ;- - Colonies = slow growing, watery white . - Mycelium = hylaline , smooth 2-3um . - Conidiophores = Irregular , close together, tufy long, - Phialides = short , plump , pear shaped ,conicol neck ,. - Phialospores = smooth , 2.8-1.8um . -
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