Includes different methods of MAS for stress resistance

1.  Utilizing MAS Procedures For Identifying Resistant
Types In Important Crops Like Rice, Sorghum, Wheat,
Cotton
Breeding For Resistance To Stresses Caused By Toxicity,
Deficiency
Pollutants Or Contaminants In Soil, Water And
Environment
1Prepared by Dr. Nidhi Dubey

2. MECHANISM OF SALINITY RESISTANCE
1. Interspecific Variation (SY100, SY50)
2. Intraspecific Variation
2Prepared by Dr. Nidhi Dubey

3. MEASUREMENT OF SALINITY RESISTANCE
1. SALINE ENVIRONMENT:
a. Non-saline field with saline irrigation
b. Microplots
c. Greenhouse environment
3Prepared by Dr. Nidhi Dubey

4. C. Greenhouse environment
i. Germination:
 Filter paper
 Antibiotic agar plate
 Pots filled with soil
 Pots filled with sand/gravel
 Hydroponics
ii. Seedling characterstics
4Prepared by Dr. Nidhi Dubey

5. ESTIMATION AND SELECTION CRITERIA OF SALINITY
RESISTANCE
I. Cell Survival
II. Germination
III. Dry matter accumulation
IV. Leaf death and Senescence
V. Leaf ion content
VI. Osmoregulation
VII. Yield
5Prepared by Dr. Nidhi Dubey

6. MINERAL
STRESS
RESISTANCE
MINERAL
DEFICIENCY
STRESS
MINERAL
TOXICITY
STRESS
MINERAL STRESS
6Prepared by Dr. Nidhi Dubey

7. MECHANISM OF MINERAL DEFICIENCY STRESS
RESISTANCE
I. Mineral redistribution
II. Efficient mineral uptake : Acidification of rhizosphere
III. Increased mineral transport
IV. Increased root/shoot ratio
V. Increased root hair density/length
7Prepared by Dr. Nidhi Dubey

8. SELECTION CRITERIA
1. Visible Deficiency
Symptoms
2. Mineral Contents of Plant
Tissues
3. Biochemical Tests
8Prepared by Dr. Nidhi Dubey

9. 9Prepared by Dr. Nidhi Dubey

10. CREATION OF MINERAL DEFICIENCY ENVIRONMENT
I. Field
II. Pots
III. Hydroponics
10Prepared by Dr. Nidhi Dubey

11. 11Prepared by Dr. Nidhi Dubey

12. SELECTION CRITERIA FOR Al and Mn TOXICITY
I. Shoot dry matter
II. Root length
III. Root weight
IV. Root deformations
12Prepared by Dr. Nidhi Dubey

13. MARKER ASSISTED SELECTION
A successful application of molecular markers to assist breeding
procedures rely on several factors:
A genetic map with molecular markers linked to the major gene(s)
or QTLs of agronomic interest;
A tight association between the markers and the major gene(s) or the
QTLs;
Adequate recombinations between the markers associated to the
trait(s) of interest and the rest of the genome and
The possibility of analyzing a large number of individuals in a time
and cost effective manner.
13Prepared by Dr. Nidhi Dubey

14. For plant breeders, the most useful application of MAS is to use
DNA-based markers for basically three purposes:
Tracing favorable allele(s) (dominant or recessive) across
generations; in order to accumulate favorable alleles,
Identifying the most suitable individuals among segregating
progenies, based on the allelic composition of a part or of the entire
genome and
Breaking the possible linkage of favorable alleles with
undesirable loci
AIMS OF MARKER ASSISTED SELECTION
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18. 18Prepared by Dr. Nidhi Dubey

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24. 24Prepared by Dr. Nidhi Dubey