Male sterility refers to the failure of plants to produce functional pollen or male gametes. There are several types of male sterility including genetic, cytoplasmic, and chemically-induced male sterility. Genetic male sterility is governed by recessive genes and can be temperature or photoperiod sensitive. Cytoplasmic male sterility is controlled by the mitochondrial genome and is not influenced by the environment. Cytoplasmic-genetic male sterility involves both nuclear and mitochondrial genes and is the most widely used system for commercial hybrid seed production in crops like maize, rice, and wheat.
Self-incompatibility refers to the inability of a plant with functional pollen to set seeds when self pollinated. It is the failure of pollen from a flower to fertilize the same flower or other flowers of the same plant.
This presentation includes, Single-locus self-incompatibility- {Gametophytic self-incompatibility (GSI) and Sporophytic self-incompatibility (SSI)},2-locus gametophytic self-incompatibility, Heteromorphic self-incompatibility,Cryptic self-incompatibility (CSI) and Late-acting self-incompatibility (LSI).
Self-incompatibility refers to the inability of a plant with functional pollen to set seeds when self pollinated. It is the failure of pollen from a flower to fertilize the same flower or other flowers of the same plant.
This presentation includes, Single-locus self-incompatibility- {Gametophytic self-incompatibility (GSI) and Sporophytic self-incompatibility (SSI)},2-locus gametophytic self-incompatibility, Heteromorphic self-incompatibility,Cryptic self-incompatibility (CSI) and Late-acting self-incompatibility (LSI).
1. STABILITY OF MALE STERILE LINES - ENVIRONMENTAL INFLUENCE ON STERILITY - EGMS - TYPES AND INFLUENCE ON THEIR EXPRESSION, GENETIC STUDIES.
2. PHOTO SENSITIVE GENETIC MALE STERILITY AND ITS USES IN HETEROSIS BREEDING
3. TEMPERATURE SENSITIVE GENETIC MALE STERILITY AND ITS USES IN HETEROSIS BREEDING
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Inability of flowering plants to produce functional pollen.
Male sterility is agronomically important for the hybrid seed production.
Onion crop provides one of the rare examples of very early recognition of male sterility cultivar Italian Red (Jones and Emsweller 1936)
Its inheritance and use in hybrid seed production (Jones
and Clarke 1943).
Since then male sterility is reported in a fairly large number of crops including vegetables.
Plant breeding methods of vegetatively propagated crops Roksana Aftab Ruhi
Vegetatively propagated crops are bred by intentionally crossing of closely or distantly related individual to produce new crop varieties or lines with desirable traits. Breeding of vegetative crops have successfully improved quality, yield, tolerance of crops to environmental pressure. Breeding helps in producing crops that are resistant to viruses, fungi and bacteria and helps in longer storage period for the harvested crop.
SELF INCOMPATIBILITY IN VEGETABLES
Mode of pollination is very important in plant breeding because it determines the genetic constitution, nature of gene action, ease in pollination control and stability of varieties after release. There are several mechanisms that promote cross pollination, among these self-incompatibility. Self incompatibility is defined as the prevention of fusion of fertile (functional) male and female gametes of the same plant (Gowers, 1989). Self incompatibility is a system where self-recognition and rejection is the rule that prevents inbreeding depression.
1. STABILITY OF MALE STERILE LINES - ENVIRONMENTAL INFLUENCE ON STERILITY - EGMS - TYPES AND INFLUENCE ON THEIR EXPRESSION, GENETIC STUDIES.
2. PHOTO SENSITIVE GENETIC MALE STERILITY AND ITS USES IN HETEROSIS BREEDING
3. TEMPERATURE SENSITIVE GENETIC MALE STERILITY AND ITS USES IN HETEROSIS BREEDING
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Inability of flowering plants to produce functional pollen.
Male sterility is agronomically important for the hybrid seed production.
Onion crop provides one of the rare examples of very early recognition of male sterility cultivar Italian Red (Jones and Emsweller 1936)
Its inheritance and use in hybrid seed production (Jones
and Clarke 1943).
Since then male sterility is reported in a fairly large number of crops including vegetables.
Plant breeding methods of vegetatively propagated crops Roksana Aftab Ruhi
Vegetatively propagated crops are bred by intentionally crossing of closely or distantly related individual to produce new crop varieties or lines with desirable traits. Breeding of vegetative crops have successfully improved quality, yield, tolerance of crops to environmental pressure. Breeding helps in producing crops that are resistant to viruses, fungi and bacteria and helps in longer storage period for the harvested crop.
SELF INCOMPATIBILITY IN VEGETABLES
Mode of pollination is very important in plant breeding because it determines the genetic constitution, nature of gene action, ease in pollination control and stability of varieties after release. There are several mechanisms that promote cross pollination, among these self-incompatibility. Self incompatibility is defined as the prevention of fusion of fertile (functional) male and female gametes of the same plant (Gowers, 1989). Self incompatibility is a system where self-recognition and rejection is the rule that prevents inbreeding depression.
Male sterility: cost effective hybrid seed prodution in Cotton.pptxNouman Bilal
Its a presentation which i present in seminar for my Msc PBG degree. In this ppt i discuss about natural ways of male sterility in cotton plant and three line i.e AB,R Line system for commercial hybrid seed prodution in cotton.
Rice (Oryza sativa L. 2n = 2x = 24) is a staple food for over half of the world's populationproviding 43% of calorie. Rice yield has experienced many fold jumps since the 1950s. This happened primarily as the result of genetic improvement and increasing harvest index by reducing plant height using the semi-dwarf genes and utilization of heterosis by producing hybrids. Heterosis is the improved or increased function of any biological quality in a hybrid offspring. An offspring exhibits heterosis if its traits are enhanced as a result of mixing the genetic contributions of its parents. Genetic basis of heterosis included overdominance, dominance, and additive effects.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. What is Male Sterility???
Male sterility is defined as “the failure of plants to produce
functional anthers, pollen, or male gametes .”(Kaul,1988)
History:-
• First time reported by Kolreuter (1763) in maize.
• Beedle (1932) and Roger (1953) - CGMS in maize
• Rhodes (1933) - CMS in maize
• Jones and Davis (1944) - CMS in onion
3.
4. Male fertile and sterile lines
Anther is full of pollens
(deep purple/blue
after iodine stain)
Anther is withered,
and has no pollen in it
Male Fertile
Male Sterile
5. Some important terms
A-line or Male Sterile
line
B-line or maintainer
line
R-line or restorer line
msms
rr
msms
Rr
msms F/S
RR
msms S
msms F
6. Classification of Male sterility
1. Genetic male sterility (GMS)
i) Temperature sensitive genetic male sterility (TGMS)
ii) Photoperiod sensitive genetic male sterility (PGMS)
iii) Transgenic male sterility (TrGMS)
2. Cytoplasmic male sterility (CMS)
3. Cytoplasmic genetic male sterility (CGMS)
4. Chemically induced male sterility (CIMS)
7. 1. GENETIC MALE STERILITY
• Governed by a single recessive gene ‘ms’.
• The ratio of sterile and fertile plants is 1:1
• This method is used for both seed and vegetatively
propagated crops for hybrid seed production.
• It is influenced by environment factors.
ms ms × Ms Ms
(male sterile) (Male fertile)
F1 Ms ms (male fertile)
F2 1 Ms Ms, 2 Ms ms, 1 ms ms
3 Male fertile 1 Male sterile
Ratio 3:1
8. i) Temperature sensitive genetic male sterility (TGMS)
In this type of GMS complete male sterility is produced by the ms gene at
higher temperature 23.3⁰C.
ii) Photoperiod sensitive genetic male sterility (PGMS)
In this type of GMS complete male sterility is produced by the ms gene
between 23-29⁰C temperature at higher photoperiod > 14 hours (long day).
>14 hours = Plants male sterile
< 14 hours= Plants male fertile
iii) Transgenic male sterility (TrGMS)
A gene introduced into the genome of an organism by recombinant DNA
technology or genetic engineering is called transgene.
Eg. Barnase/ Barstar system (Bar gene of Bacillus amyloliquefaciens)
Barnase = Male sterile
Barstar = Male fertile
Application of Genetic Male Sterility in Plant Breeding
Applicable in production of hybrids in both vegetatively propagated crops and
crops important for fruit or seed yield.
eg. Castor = USA
Pigeon pea = India (variety released- ICPH-8, 1991)
9. 2. CYTOPLASMIC MALE STERILITY
• Controlled by plasma gene contributed by female parent. (Mitochondrial
DNA, Cp DNA and Plasmid)
• This method is using only in vegetative propagated crops.
• It is highly stable i.e. not influenced by environmental factors.
Genotypes of CMS line
– With normal fertile cytoplasm
(B – line; fertile)
– With sterile cytoplasm
(A – line; sterile)
msms F
R / r
msms S
R / r
10. Sterility maintenance
msms Smsms F
R / r
A – line
(sterile)
B – line
(fertile)
All male sterile
progenies
msms S
R / r
X R / r
Application of Cytoplasmic Male Sterility in Plant Breeding
Production of hybrids in ornamental crops and vegetatively propagated crops,
where grain or fruit is not the economic product.
Examples – Ornamental plants, potato, onion etc.
11. 3. CYTOPLASMIC – GENETIC MALE STERILITY
• Controlled by induction of both nuclear and plasma genes.
• First reported by Jones & Esweller (1937) in onion.
• 1944 – Jones first found ‘S’ type from USDA stock
and Roger in Texas found ‘T’ type
• The fertility restorer gene Rf, is dominant.
A-line or Male Sterile line
B-line or maintainer line
R-line or restorer line
12. Male Sterility Maintenance
Fertility Restoration
msms S
rr
msms S
Rr
msms S
RR
msms S
rr
msms F
rr
msms S
rr
msms F
RR
Male sterile
(A – line)
All fertile
progenies
A – line
(sterile)
B – line
(Fertile)
All male
sterile
progenies
or
Male fertile (R – line)
X
X
13. Application of Cytoplasmic-Genetic Male Sterility
in Plant Breeding
• CGMS is most popular MS system for commercial
utilization due to inherent disadvantages of GMS and
CMS.
• It is used in commercial production of hybrid seeds
maize, sorghum, bajra, sunflower, rice and wheat.
14. 4. Chemically induced male sterility (CIMS)
Many chemicals affect the function of male reproductive organs in plants,
these compounds called chemical hybridizing agents (CHA) or male
gametocide.
15. Crop Cytoplasm Restore genes Remarks
1. Maize (Z.m.) Cms – C
Cms – S
Cms – T
Rf4
Rf3
Rf1, Rf2
Spontaneous reversion
Commercially used
• Most commercially used
• Reduce yield (2-4%)
• Susc. To southern corn blight
& sheath blight
Sorghum
(S.bicolor)
Milo
(S.bicolor)
MSc (one) Commercially used
Bajra
(Penmisteum
americanum)
Tifton (P. amc.)
Ludhiana (P. anc)
A1
A2
Commercially used
Little used
Most well studied CMS system in crop plants
A number of cytoplasms known in maize :
- Texas (T)
- USDA (S)
- Charrua (C)
- Normal (N)
- NA (North America)