- Monoclonal antibodies are monospecific antibodies produced by identical immune cells that are clones of a single parent cell. They bind to the same epitope.
- George Kohler and Cesar Milstein first developed the hybridoma technique in 1975 to produce monoclonal antibodies in large quantities.
- There are four main types of monoclonal antibodies: murine, chimeric, humanized, and human. Therapeutic monoclonal antibodies are used to treat conditions like cancer, autoimmune diseases, infections, and asthma. They have revolutionized medicine and many new applications are still being developed.
Immunological Basis of Graft Rejection
Transplantation in immunology, refers to the act of transferring cells, tissues, or organs from one site to another.
A healthy organ, tissue, cells which is provided by a donor is termed as a GRAFT.
The immune system has evolved elaborate and effective mechanisms to protect the organism from attack by foreign agents, and these same mechanisms cause rejection of grafts from anyone who is not genetically identical to the recipient.
Immunological Basis of Graft Rejection
Transplantation in immunology, refers to the act of transferring cells, tissues, or organs from one site to another.
A healthy organ, tissue, cells which is provided by a donor is termed as a GRAFT.
The immune system has evolved elaborate and effective mechanisms to protect the organism from attack by foreign agents, and these same mechanisms cause rejection of grafts from anyone who is not genetically identical to the recipient.
This presentation clearly describes what are immunoglobulins, their types, structure and how they get diversified into different isotopes to fight with foreign antigens.
Antibody purification – what you need to know to use antibodies effectivelyExpedeon
In this webinar Dr Andy Lane discusses the various methods available for purifying antibodies from different sources, and explains why it is vitally important to understand how your antibodies have been purified to know what you can do with them, either within assays or for further processing such as conjugation to dyes and enzymes.
This presentation clearly describes what are immunoglobulins, their types, structure and how they get diversified into different isotopes to fight with foreign antigens.
Antibody purification – what you need to know to use antibodies effectivelyExpedeon
In this webinar Dr Andy Lane discusses the various methods available for purifying antibodies from different sources, and explains why it is vitally important to understand how your antibodies have been purified to know what you can do with them, either within assays or for further processing such as conjugation to dyes and enzymes.
MAINLY FOCUSING ON MONONCLONAL ANTIBODIES,TYPES OF IT, METHOD OF PRODUCTION, FDA APPROVED MABs, & HOSPITAL COMMONLY USED MONOCLONAL ANTIBODIES, DISSCUING THE ECONOMICS AS WELL.
Monoclonal antibody
# Definition
# A general representation of the method used to produce monoclonal antibodies
#Applications
# Monoclonal antibodies for cancer.
#MAbs approved by the FDA for cancer include:
# Factor affecting monoclonal antibody immunogenicity
#Monoclonal Antibodies : 4 Types
# Monoclonal Antibodies : 4 Types images
Monoclonal Antibody-Preparation & Application - MPH201T.pptxRAHUL PAL
Monoclonal antibodies (mAbs) are proteins produced by a single type of B cell. They are identical to each other and recognize a specific antigen. Antigens are molecules that the body's immune system recognizes as foreign. When an antigen binds to a monoclonal antibody, it triggers a series of reactions that can lead to the destruction of the antigen.
Monoclonal antibodies can be used to treat a variety of diseases, including cancer, autoimmune diseases, and infections. They are also used in research and diagnostics.
Topics included :- Introduction to monoclonal antibody; Principle for creation of hybridoma cells and steps involved in it; Second generation monoclonal antibodies; Advantages, disadvantages and applications (Diagnostic and therapeutic) of MAbs.
Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This process starts by injecting a mouse (or other mammal) with an antigen that provokes an immune response.
Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This process starts by injecting a mouse (or other mammal) with an antigen that provokes an immune response.
Hybridoma
Hybridomas are cells that have been engineered to produce a desired antibody in large amounts, to produce monoclonal antibodies.
Monoclonal antibodies can be produced in specialized cells through a technique now popularly known as hybridoma technology.
Hybridoma technology was discovered in 1975 by two scientists, G. Kohler and C. Milstein, were awarded Noble prize for physiology and medicine in 1984.
The Gram stain is a fundamental technique in microbiology used to classify bacteria based on their cell wall structure. It provides a quick and simple method to distinguish between Gram-positive and Gram-negative bacteria, which have different susceptibilities to antibiotics
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
1. - Aarati K.C
- Chhabi Acharya
B.Pharmacy
HOPE Int’l College
Monoclonal Antibodies'
Popular Drugs
2. We will discuss about:
• Introduction
• History
• Types (with example)
• Pharmacokinetic profile
• Therapeutic uses (with specific drug)
• Future Aspect
• Conclusion
3. Introduction:
• Monoclonal antibodies (mAb or moAb)
are monospecific antibodies that are the same
because they are made by identical immune cells
that are all clones of a unique parent cell.
• Monoclonal antibodies have monovalent affinity, in
that they bind to the same epitope.
• Monoclonal antibodies that specifically bind to
substance; they can then serve to detect or purify
that substance.
• This has become an important tool
in biochemistry, molecular biology and medicine.
4. History:
• 1975: Hybridoma
Technology
George Kohler and Cesar
Milstein devised a method
to obtain large amounts of a
mAb
• 1984: Noble prize for
medicine
• 1988: Greg winter et. al
pioneered the techniques to
humanize monoclonal
antibodies
Cesar Milstein & George Kohler
5. Types of mAb:
• Murine
• Chimeric mAb
• Humanized mAb
• Human mAb
6. 1.Murine:
• Derived from mice murinae.
• Patients treated with murine mAbs develops human
antimouse antibodies(HAMA)
• Rapid clearance of the mAbs.
• Poor tumor penetration.
• Hypersentivity reaction.
7. 2.Chimeric mAbs:
• Antigen binding parts(variable region) of mouse Ab
with effector parts (constant region)of human
• E.g.:
- Infliximab
- Abciximab
- Rituximab
8. 3.Humanized mAb:
• Human antibody with complimentary determining
region(CDR) or hyper variable region from non
human sources
• E.g.:
- Daclizumab
- Trastuzumab
9. 4. Human mAb:
• Human monoclonal antibodies are
produced using transgenic mice.
• Human monoclonal antibodies are
produced by transferring human
immunoglobulin genes into the murine
genome, after which the transgenic mouse
is vaccinated against the desired antigen,
leading to the production of monoclonal
antibodies, allowing the transformation of
murine antibodies in vitro into fully human
antibodies.
10. Pharmacokinetics:
• Routes of administration:
- Subcutaneously: Rituximab, trastuzumab, adalimumab
- Intramuscularly: Palirizumad
• Half-life:
- Chimeric: 4-15 days
- Humanized: 3-24 days
- Recombinant human:11-24days
- Human antimouse antibody (HAMA) response
develops 7-10 days following exposure to murine
antibody.
12. 1.Immuno suppression:
• Muromonab: CD-3-murine mAbs
• 1st
mAb approved for clinical use in humans.
• Act on CD3 receptors.
• Apoptosis of the T-cell.
• Prevents graft rejection in renal transplant.
• ADRs: Skin reaction, fever, chills, headaches,
nausea, and diarrhea etc.
13. Autoimmune disease
• Epratuzumab:
- Humanized antibodies that binds to the glycoprotein
CD22 of mature and malignant B-cells.
• Efalizumab:
- Humanized antibody that binds with CD11 a subunit of
lymphocytes function associated antigen 1.
- Therapeutic uses: Plague psoriasis.
- ADR: Bacterial sepsis, viral meningitis, invasive fungal
disease
14. 2.Magligencies:
• Rituximab: First chimeric IgG-I mAb Mechanism of
action: Directed against CD 20 on B cells.
• Cetuximab: Chimeric Ab Directed against EGFR
Inhibits tumor growth
- Therapeutic use: Metastatic colorectal cancer, head
and neck cancer.
• Bevacizumab: Humanized mAb
- MoA: Directed against VEGF Blocks VEGF in
neovascularisation.
15. - Therapeutic uses: Metastatic colorectal ca, Pancreatic
ca, Breast cancer Prostate cancer.
• Trastuzumab: Humanized Ab.
- Therapeutic uses: Metastatic breast cancer.
17. 4.Asthma
• Omalizumab: Humanized Ab
• MOA: - Binds IgE
- Interaction with
basophils and mast cells.
• Therapeutic uses: Asthma in
adults and adolescents
refractory to inhaled
corticosteroid therapy.
18. 5.Osteoporosis:
• Denosumab: Human mAb
• Act against rank ligand
• Approved by FAD for use in
postmenopausal women with
risk of osteoporosis.
• ADR: Urinary tract infection,
constipation, rashes and joints
pain.
19. Future aspect:
• Lpilimumab: Human monoclonal antibodies.
• Undergoing clinical trials treatment of melanoma
• Zanolimumab: CD4 specific mAb
• Cutaneous T-cell lymphoma
• Phase iii clinical trial
• Others uses: Diagnostics:
• HIV diagnostics kit
• A mAb can be used to detect pregnancy only 14
days after conception.
• Other mAb allow rapid diagnosis of hepatitis.
20. Conclusion:
• MAbs are highly specific Abs produced by a clone
of single hybrid cells formed by fusion of B cell
with the tumor cell.
• The hybridoma formed yields higher amount of
MAbs. MAbs can be produced in vitro and in vivo .
• Recombinant DNA technology, genetic
engineering and transgenic animals are used to
produce humanized MAbs or pure human MAbs,
with fewer ADRs Used for treatment of cancer,
autoimmune disorders, graft rejections, infections,
asthma etc.
21. References:
• Dr. Haramjit Singh, Monoclonal antibodies and
their role in pharmacology, 19th
July 2012
• http://www.imgt.org/IMGTrepertoire/GenesClinical/mon
• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC287422/