Enable the tracking of contamination of in Vitro culture media in the whole process of plant propagation and helps to decrease the spread of plant parasites. This link between inventory and bulk media culture allow detect the origin of contamination (which bulk media culture was used, when was prepared, technician in charge,…) and localize other inventories contaminated.

1. L i m a - P e r u
15 March, 2021
Use Case: Linking in Vitro inventories with bulk
media culture to enable tracking of contamination.
Matija Obreza (Crop TRUST)
Juan Carlos Alarcon (CIMMYT)
Juan Carlos Guerrero (CIAT)
Edwin Rojas (CIP)

2. Agenda
• Use Case Description
• Steps with use case images
• Attributes used to register the Bulk Medium Culture
• Current GRIN-Global Schema with Inventory & related tables
• Tentative proposal in the GG schema

3. CIP Use Case Description
Title: Linking in Vitro GRIN-Global inventories with bulk media culture.
Purpose of linking: Enable the tracking of contamination of in Vitro culture
media in the whole process of plant propagation and helps to decrease the
spread of plant parasites. This link between inventory and bulk media culture
allow detect the origin of contamination (which bulk media culture was used,
when was prepared, technician in charge,…) and localize other inventories
contaminated.
Gap: When migrating in Vitro inventories from in-house system to the GRIN-
Global database schema no way to register and link the numeric ID of the bulk
media with theirs related attributes.
Vocabulary:
- In Vitro inventory: Is a clonal form of germplasm conservation for each inventory of the collection.
- Bulk media culture: Is an in Vitro media culture normally prepared by liters.

4. Steps with use case images
01
Initiate bulk media preparation in
the laboratory using for example
macro, micronutrients, vitamins, gel
agent, mineral salts and water

5. Dispense the bulk media culture into the
tubes
02
03
Register and print one label for
the rack of tubes of step 02, the
label only show the ID of the
bulk media culture
Barcode
label with ID
bulk media
culture and
tubes only
with
medium

6. 04
05 06
Scanning previous
inventories and
link the bulk of
media culture in the
database
Printing new
barcode
inventories
Attaching the new
Pack of labels
to the rack

7. New rack with new media culture
Old rack 07 Initiate In Vitro propagation to refresh
germplasm inventories

8. 08
Visual inspection to evaluate
contamination for later if it is
necessary linking it with the
origin of media bulk
preparation

9. Table: Bulk Media Culture Attributes
In Liters
Attributes used to register the Bulk Medium Culture

10. Current GRIN-Global
Schema with Inventory &
related tables

11. Tentative proposal in the GG schema - 1 How to resolve ?
Is needed a module to handle laboratory supplies and chemicals and inside there link bulk
media culture with inventories.
Treatments:
In the field  fertilizer or pesticides/insecticides applied, or pollinators, etc. (plots)
Screen house  fertilizer or pesticides/insecticides applied, or pollinators, etc. (pots)
In Vitro  media culture (glass tubes)
Cryo  media culture (cryo vials)

12. Tentative proposal in the GG schema - 2 How to resolve ?

13. Tentative proposal in the GG schema - 3 How to resolve ?

14. The International Potato Center (known by its Spanish acronym CIP) is
a research-for-development organization with a focus on potato, sweetpotato,
and Andean roots and tubers. CIP is dedicated to delivering sustainable
science-based solutions to the pressing world issues of hunger, poverty,
gender equity, climate change and the preservation of our Earth’s fragile
biodiversity and natural resources.
www.cipotato.org
CIP is a member of CGIAR
CGIAR is a global agriculture research partnership for a food secure future. Its
science is carried out by the 15 research centers who are members of the
CGIAR Consortium in collaboration with hundreds of partner organizations.
www.cgiar.org