This document discusses the need to link treatment IDs to inventory IDs in genebank inventory systems. It notes that the current systems do not properly track treatments applied to samples during conservation processes like in vitro culture or cryopreservation. The document proposes linking treatment and inventory IDs in barcodes, registering container and treatment details, and implementing a tracking and traceability system to integrate software for treatments with the genebank management platform. This would improve tracking samples through different conservation treatments and regenerating inventories.

1. L i m a - P e r u
26 October, 2022
Genebank Inventory vs GRIN-Global inventory
Edwin Rojas (CIP)
IT Specialist

2. Seed inventories In Vitro inventories Cryo inventories
Genebank inventory vs GRIN-Global inventory

3. Seed inventory In Vitro inventory
Cryo inventory
• Nutritive
substance
• Protective substance
• Aluminum paper
• Nitrogen liquid
• Container
• Plant genetic
resource
• Identification
Genebank inventory vs GRIN-Global inventory

4. Genebank inventory vs GRIN-Global inventory
.
Seed inventory in processes
collection
In Vitro inventory in
processes collection
Cryo inventory in
processes collection
Container
Aluminum envelope (6x8,
8x12, 21.5x17 cm), pot
(6,8,10 inch), paper envelope
(18.2x10.2, 8.6x5.8 cm), petri
dish, jar
Glass tube (13x100,
16x125, 18x150, 25x150
mm), magenta, petri dish
Cryo tube, Petri dish
(standard, deep),
Erlenmeyer flasks
(50 and 25 mL),
Glass tube (25x150
mm)
Plant genetic resource
Seed, sprout, plant, mini-
tuber, lyophilized tissue Plantlet, plant shoot tip
Plant shoot tip,
plantlet
Identification Barcode label Barcode label Barcode label
Nutritive substance -
Culture medium in
which plantlets are
grown (>30 types)
Culture medium in
which plantlets are
grown (>8 types)
Protective substance +
nitrogen
Tissue culture
protection of
nitrogen (2 types)
Aluminum paper
Piece of aluminum
in which material are
hold

5. Genebank In Vitro Laboratories/Areas
Stock of
chemicals and
supplies
Media Preparation Laboratory Conservation Chambers
Cold chamber
Active chamber
Propagation Area
Rack of tubes
with old genetic resources
Preparation
Area
Rack of tubes
with media
Rack of tubes
with new genetic resources
Printer
Laminar Flow
Cabinet
1
2
3
4
Points of IDs registration
and relations

6. Steps with use case images (Media Preparation Laboratory)
01
Initiate bulk media preparation in
the laboratory using for example
macro, micronutrients, vitamins, gel
agent, mineral salts and water

7. Dispense the bulk media culture into the
tubes
02
03
Register and print one label for
the rack of tubes of step 02, the
label only show the ID of the
bulk media culture
Barcode
label with ID
bulk media
culture and
tubes only
with
medium

8. 04
05 06
Scanning previous
inventories and
link the bulk of
media culture in the
database
Printing new
barcode
inventories
Attaching the new
Pack of labels
to the rack

9. New rack with new media culture
Old rack 07 Initiate In Vitro propagation to refresh
germplasm inventories

10. 08
Visual inspection to evaluate
contamination for later if it is
necessary linking it with the
origin of media bulk
preparation

11. Different treatments are applied during
storage for conservation and regeneration
Treatments:
• In the field  fertilizer or pesticides/insecticides applied, or pollinators, etc. (plots)
• Screen house  fertilizer or pesticides/insecticides applied, or pollinators, etc. (pots)
• In Vitro  media culture (glass tubes)
• Cryo  media culture, protective substance (glass tubes and cryo vials)
Plant Collection
in Bench
Plant Collection
in Field

12. Modeling treatments and inventories
Treatment
Base
Inheritance
Treatment
Sample
Chemicals
&
Supplies
Inventory
Treatment
Attributes
Inventory
Action
Composition
Composition
1..*
Multiplicity Association
Reflexive Association

13. Bulk Medium Attributes
In Liters
Attributes used to register Bulk Medium for in Vitro

14. Conclusions
• The treatment ID should be linked to the Inventory ID.
• Is needed a software module to handle laboratory supplies and chemicals.
• The new regenerated inventory ID should be linked to the old conserved inventory ID.
• The barcode label should print the inventory ID, Treatment ID and not only
the treatment name.
• The container type for inventory storage or inventory for regeneration should be
registered and not only for distribution.
• One innovative approach to responding to these challenges is the implementation
of Tracking and Traceability Systems (also named workflow systems) to
integrate the software module for treatments with GG platform.

15. The International Potato Center (known by its Spanish acronym CIP) is
a research-for-development organization with a focus on potato, sweetpotato,
and Andean roots and tubers. CIP is dedicated to delivering sustainable
science-based solutions to the pressing world issues of hunger, poverty,
gender equity, climate change and the preservation of our Earth’s fragile
biodiversity and natural resources.
www.cipotato.org
CIP is a member of CGIAR
CGIAR is a global agriculture research partnership for a food secure future. Its
science is carried out by the 15 research centers who are members of the
CGIAR Consortium in collaboration with hundreds of partner organizations.
www.cgiar.org