Gene$c varia$on in resistance to Pacific oyster mortality syndrome was studied through trials of oyster families. There was large varia$on in survival rates both between families and within families. Selec$ng the best families and individuals for breeding could improve average survival rates from 47% to 70-80%, depending on disease severity. Further gene$c studies are needed to beZer understand inheritance of resistance.

1. Gene$c
varia$on
in
resistance
to
Pacific
oyster
mortality
syndrome
Peter
Kube
(CSIRO)
Ma?hew
Cunningham
(ASI)
Mike
Dove
(NSW
DPI)
Wayne
O’Connor
(NSW
DPI)
Paul
Hick
(NSW
DPI)
Presenta$on
to
Shellfish
Futures
2012
17-­‐19
August
2012
Port
Arthur
Tasmania

3. Tray
1
Tray
9

4. Woolooware Bay, Georges River (24 May 2012)
ASI
gene$cs
trial
University
of
Sydney
trial

5. Large
varia$on
in
survival
along
rack
34%
32%
33%
20%
17%
14%
33%
32%
20%
43%
43%
43%
71%
37%
54%
69%
58%
53%
49%
62%
74%
58%
82%
78%
51%
56%
61%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9
AVERAGE
SURVIVAL
=
47%
Survival
aZer
14
days
(26
April
2012)

6. Tray
1
Tray
9

7. Some
varia$on
in
survival
across
rack
AVERAGE
SURVIVAL
=
47%
Survival
aZer
14
days
(26
April
2012)
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9
4 4 %
4 4 %
4 6 % 4 6 %
4 6 %
4 4 %
4 2 % 4 2 %
4 2 %
4 2 %
42% 4 2 %
5 3 % 5 3 %
5 3 %
4 9 % 4 9 %
4 9 %
4 4 %
44% 4 4 %
4 6 %
46% 4 6 %
5 3 %
53% 5 3 %
4 9 %
49% 4 9 %
5 3 %
4 9 %
4 4 %
4 6 %
4 2 %
5 3 %
4 9 %
4 4 %
4 6 %
4 2 %
4 4 %
5 3 %
4 9 %
4 6 %
4 2 %

8. 0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
3
4
2
8
4
2 2
1
0
2
1
4
5 8
1
5
3
2
5
1
2
9
2
5
1
2
4
7
1
3
4
3
2
6
1
8 3
1
9
1
6
3
0
2
0
2
3
2
4
3
6 7 9 5
1
1
1
7
3
5
4
9
5
0
4
1
4
8 1 4
2
2 6
2
7
3
3
S
U
R
V
IV
A
L
EB
V
FAMILY
NUMBER
Estimated
Breeding
Value
for
Survival:
2011
Year
Class
Families
Heritability
=
0.38
þ

9. A
group
of
cousins
þ
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
34
28
42
2
10
21
45
8
15
32
51
29
25
12
47
13
43
26
18
3
19
16
30
20
23
24
36
7
9
5
11
17
35
49
50
41
48
1
4
22
6
27
33
SURVIVAL
EBV
FAMILY
NUMBER
Estimated
Breeding
Value
for
Survival:
2011
Year
Class
Families

10. A
group
of
cousins
ý
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
3
4
2
8
4
2 2
1
0
2
1
4
5 8
1
5
3
2
5
1
2
9
2
5
1
2
4
7
1
3
4
3
2
6
1
8 3
1
9
1
6
3
0
2
0
2
3
2
4
3
6 7 9 5
1
1
1
7
3
5
4
9
5
0
4
1
4
8 1 4
2
2 6
2
7
3
3
S
U
R
V
IV
A
L
EB
V
FAMILY
NUMBER
Estimated
Breeding
Value
for
Survival:
2011
Year
Class
Families

11. FAMILY
2009003 FAMILY
2009025 FAMILY
2009027
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
IN
D
IV
ID
U
A
L
S
U
R
V
IV
A
L
E
B
V
Within
Family
Variation
(3
selected
families
only)

12. Survival:
present
popula$on
34%
32%
33%
20%
17%
14%
33%
32%
20%
43%
43%
43%
71%
37%
54%
69%
58%
53%
49%
62%
74%
58%
82%
78%
51%
56%
61%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9
AVERAGE
SURVIVAL
=
47%
Survival
in
2012

13. Survival:
best
families
SURVIVAL
IMPROVEMENT
=
47%
to
~70%
Predicted
survival
in
2014
60%
59%
60%
52%
50%
48%
60%
59%
52%
66%
66%
66%
83%
62%
72%
81%
75%
72%
69%
77%
84%
75%
89%
87%
71%
73%
77%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

14. Survival:
best
individuals
and
best
families
SURVIVAL
IMPROVEMENT
=
47%
to
~80%
Predicted
survival
in
2014
73%
73%
73%
68%
67%
65%
73%
73%
68%
77%
77%
77%
88%
75%
82%
88%
83%
81%
79%
85%
90%
83%
93%
91%
81%
82%
84%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

15. Survival:
higher
disease
severity
ASSUMED
AVERAGE
SURVIVAL
=
25%
18%
17%
17%
11%
9%
7%
17%
17%
10%
23%
23%
23%
38%
19%
29%
37%
31%
28%
26%
33%
39%
31%
43%
42%
27%
30%
32%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

16. Survival:
best
families
and
higher
disease
severity
SURVIVAL
IMPROVEMENT
=
25%
to
40%
Predicted
survival
in
2014
28%
27%
28%
17%
14%
12%
28%
27%
17%
36%
36%
37%
60%
31%
46%
58%
49%
45%
41%
53%
63%
50%
69%
67%
44%
47%
52%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

17. Survival:
best
individuals
from
best
families
and
higher
disease
severity
SURVIVAL
IMPROVEMENT
=
25%
to
50%
Predicted
survival
in
2014
42%
40%
41%
33%
31%
29%
41%
41%
33%
48%
48%
48%
66%
44%
55%
65%
57%
54%
52%
60%
68%
58%
73%
71%
53%
56%
59%
Replicate 1 Replicate 2 Replicate 3
Tray 1 Tray 2 Tray 3 Tray 4 Tray 5 Tray 6 Tray 7 Tray 8 Tray 9

18. The
main
messages
• Gene$c
selec$on
will
improve
resistance
• But
it
is
most
likely
a
medium
term
solu$on
• S$ll
lots
to
learn
about
the
gene$c
inheritance
of
this
disease

19. Acknowledgements
• The
trial
site
was
provided
by
Drakes
Oysters
and
this
work
would
not
have
been
possible
without
the
assistance
of
Bob
Drake,
Ted
Drake
and
Len
Drake
• This
study
was
done
as
part
of
the
Seafood
CRC
and
was
made
possible
with
funding
assistance
by
the
Seafood
CRC
• Richard
Whiengton
and
Ika
Paul-­‐Pont
(University
of
Sydney)
have
helped
out
by
generously
sharing
their
knowledge
and
experience