This document is a laboratory report summarizing a test to determine cholesterol levels in a serum sample using the CHOD-POD enzymatic method. The student introduces cholesterol and its importance, describes the test methodology which uses cholesterol oxidase and peroxidase enzymes to produce a colored complex proportional to cholesterol concentration. The student then provides their test results showing the sample cholesterol level was within the normal range, discusses clinical significance of cholesterol levels and limitations of the test.
Estimation of Blood Urea Nitrogen by Dr. TehmasTehmas Ahmad
Lecture/Demonstration of Biochemistry Practical of Blood Urea Nitrogen estimation in serum Delivered on 11-04-2018 to 2nd year MBBS students of Bannu Medical College, Bannu.
Estimation of serum triglycerides by Dr. TehmasTehmas Ahmad
Estimation of Serum Triglycerides, Practical demonstration lecture for 2nd year MBBS students of Bannu Medical College, Bannu. Lecture delivered on 13/03/2018
Estimation of Blood Urea Nitrogen by Dr. TehmasTehmas Ahmad
Lecture/Demonstration of Biochemistry Practical of Blood Urea Nitrogen estimation in serum Delivered on 11-04-2018 to 2nd year MBBS students of Bannu Medical College, Bannu.
Estimation of serum triglycerides by Dr. TehmasTehmas Ahmad
Estimation of Serum Triglycerides, Practical demonstration lecture for 2nd year MBBS students of Bannu Medical College, Bannu. Lecture delivered on 13/03/2018
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
This slide briefly imparts the knowledge of Amylase and Lipase enzymes. The clinical importance, calculation, concentration, sources and principle of amylase estimation are the major components of uploaded slide.
CLINICAL CONSIDERATIONSNoncommunicating (obstructive) hydrocephalus occurs more frequentlyCSF of ventricles unable to reach subarachnoid spaceProduction of CSF continuesGyri are flattened against inside of skullIf skull is still pliable head may enlarge
Analysis of CSF, Gucose, Protien, Albumin, Globulin, Function of CSF, Secretion of CSF, Absorption of CSF, Bacterial Infection, Clinical Significance, Normal Composition of CSF, Microscopic examination of CSF, COOMASSIE BRILLIANT BLUE(CBB), REVERSE BIURET METHOD
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
This slide briefly imparts the knowledge of Amylase and Lipase enzymes. The clinical importance, calculation, concentration, sources and principle of amylase estimation are the major components of uploaded slide.
CLINICAL CONSIDERATIONSNoncommunicating (obstructive) hydrocephalus occurs more frequentlyCSF of ventricles unable to reach subarachnoid spaceProduction of CSF continuesGyri are flattened against inside of skullIf skull is still pliable head may enlarge
Analysis of CSF, Gucose, Protien, Albumin, Globulin, Function of CSF, Secretion of CSF, Absorption of CSF, Bacterial Infection, Clinical Significance, Normal Composition of CSF, Microscopic examination of CSF, COOMASSIE BRILLIANT BLUE(CBB), REVERSE BIURET METHOD
CholesLo shows clinical significance in
helping reduce plasma cholesterol and
homocysteine levels and therefore affects
favourably the risk of subsequent development
of cardiovascular disease. Furthermore, our
findings suggest that the dose required to cause
such improvements in plasma lipid profile is
safe enough to be considered for use in general
population.
B. Pharm. (Honours) Part-IV Practical, Pharmacology-III, MANIKImran Nur Manik
3. Pharmacology-III: (Marks-30)
Estimation of glucose in blood in normal condition and after administration of insulin; biological assay of digitalis, histamine and insulin; microbiological assay of antibiotics and vitamins; spectrophotometric estimation of blood pigments; toxicity test of the drugs like, phenobarbitone, nikethamide, some antineoplastic drugs, pilocarpine, etc.
Estimation of glucose in blood in normal condition and after administration of insulin; biological assay of digitalis, histamine and insulin; microbiological assay of antibiotics and vitamins; spectrophotometric estimation of blood pigments; toxicity test of the drugs like, phenobarbitone, nikethamide, some antineoplastic drugs, pilocarpine, etc.
Dyslipidemia is a medical condition that refers to an abnormal level of blood lipids.
The most common type of dyslipidemia is hyperlipidemia or high lipid levels.
less common form of dyslipidemia: hypolipidemia, abnormally low lipid levels.
Dyslipidemias can affect any lipid parameters including LDL cholesterol levels, HDL cholesterol levels, triglycerides, or a combination of these lipids.
Two categories:
Primary dyslipidemia
Secondary dyslipidemia
Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids.
Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions.
Biochemistry deals with body substance like enzymes, carbohydrates, amino acids, fats, proteins, hormones, DNA, RNA, pigments etc.
The major objective of biochemistry is the complete understanding of all chemical processes associated with living cells at the molecular level. Some of the objectives can be listed as follows:
1. Isolation, structural elucidation and the determination of mode of action of biomolecules.
2. Identification of disease mechanisms.
3. Study of in born errors of metabolism.
4. Study of oncogenes in cancer cells.
5. The relationship of biochemistry with the genetics, physiology, immunology, pharmacology, toxicology etc.
Biochemistry is related to almost all the life sciences and without biochemistry background and knowledge, a through understanding of health and well-being is not possible.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
2. Introduction:
Cholesterol is a member of a large group of substances called steroid, which include vitamin D.
Cholesterol is an essential component of cell membrane, brain and nerve cells, and bile, which
helps the body absorb fats and fat soluble vitamins. The body uses cholesterol to make vit.D
and various hormones, such as estrogen, testosterone, and cortisol. The body can produce all
the cholesterol that it is needs, but it also obtains cholesterol from food. The recommended
daily dietary intake is 300mg.
Cholesterol is found in all cells of the body . The adrenal gland contain 6% cholesterol (by
weight). It is a precursor of the adrenal and sex hormones . The brain and spinal cord contain
2% cholesterol :in these tissues cholesterol forms part of the lipid "insulation "which separate
individual nerve fibers . The precise function of cholesterol in all other cells is unknown , but it
is may be related to the structure and permeability of the cell membrance . Cholesterol may be
synthesized from two - carbon units (acetyl - CoA) in many body tissues , particularly liver ,
intestine , and skin . Most cholesterol is excreted from the body via the bile , liver cells oxidize
the molecule by adding hydroxyl and carboxyl groups (the ring structure remain intact )to from
cholic acid. These are excreted in the bile where they are instrumental in the absorption of fasts
, including cholesterol it self, from the diet . Cholesterol serves as a precursor of other steroids ,
bile acids , vitamin D3 biosynthesis . Measurement of total cholesterol includes both the
esterified and free forms of the steroid . In plasma or serum, two - thirds of the total
cholesterol exists in the esterified form , with the rest in the free forms . The measurement of
serum cholesterol is one of the most frequently performed tests in the clinical chemistry .
It is important to do a quantitative analysis of the cholesterol levels in the blood for evaluation
of the coronary arterial occlusion, artherosclerosis, liver function, intestinal absorption and
adrenal disease. Total serum cholesterol is the measurement routinely taken. Doctors
sometimes order a complete lipid profile to better evaluate the risks for atherosclerosis
(coronary artery disease, or CAD).
Method:
CHOD – POD Enzymatic method, End Point. The cholesterol present in the sample originates a
colored complex, the intensity of the color formed is proportional to the cholesterol
concentration in the sample.
3. Principle Theory:
Cholesterol esterases (CHE) hydrolyze the cholesterol esters into free cholesterol. Cholesterol
oxidase (CHOD) oxidizes the cholesterol into cholest-4-en-3-one and hydrogen peroxide.
Hydrogen peroxide reacts with a mix. of 4- aminoantipyrene and phenol in the presence of
peroxidase enzyme (POD) & converts the reactants into a red quinone dye.
-Cholesterol Ester + H2O—cholesterol esterase—→ Cholesterol + Fatty Acids
-Cholesterol +O2—cholesterol oxidase—→ Cholest-4-en-3-one + H2O2
-H2 O2 + 4 aminoantipyrene + Phenol →peroxidase—→ Quinoneimine dye + 4H2O
Absorbance of quinoneimine is directly proportional to the cholesterol concentration, when
measured at 505 nm.
Preparation:
Working reagent; dissolve the contents of one vial R 2 Enzymes in one bottle of R1 Buffer. Cap
and mix gently to dissolve contents. This reagent is stable for 4months at 2 – 8°c or 40days at
15 – 25°c.avoid direct sunlight.
Samples; serum or plasma.
Procedure:
1.Assay conditions,
Wavelength 505nm(500 – 550)
Cuvette 1cmlight path
Temperature 37°c/15-25°c
2.Adjust the instrument to zero with distilled water.
4. 3.pipette into a cuvette:
sample Standard Blank
1.0 1.0 1.0 WR (mL)
-- 10 -- Standard(µL)
10 -- -- Sample(µL)
4.mix and incubate for 5min at 37°c or 10min at room temperature.
5.Read the absorbance of samples and standard, against the blank. The color is stable for
60minutes.
Calculation:
Cholesterol concentration in the sample can be calculated using the following formula:
A(sample)/A(standard) x 200(standard conc:)=mg/dlcholesterol in sample.
Example: If the absorbance of sample is 0.200 and the absorbance of standard is 0.18. The
calculation shall be: 0.200 / 0.180 x 200 = 222.22 mg/dl
Reference value Reference range varies from population to population; therefore, each
laboratory establishes its own normal range.
Normal – less than 200mg/dl
Borderline- 200-239mg/dl
High – 240mg/dl and above.
5. Observations and results:
Conc: of standard 200mg/dl or 5.1mmol/l
Optical density(OD) of standard 0.485
Conc: of sample ?
OD of sample 0.262
OD of blank 0.137
Calculating concentration of sample:
Conc: of sample= 200mg x 0.262/0.485 =108.041mg/dl. Or (108.041mg/dl x
0.259) = 2.798mmol/L.
-Therefore the serum sample cholesterol level is within the normal range
Discusions and clinical importanse:
Hypercholesterolemia : Increases of total serum cholesterol levels are found most
characteristically in the primary hyper lipoproteinaemas type I-V, and in the nephrotic
syndrome, myxoedema , obstructive jaundice and diabetes mellitus , but less consistently and
markedly in some other conditions . In the nephrotic syndrome when oedema is present values
up to (600 - 700 mg/100 ml) are common and may occasionally reach and exceed (1000 mg/100
ml) . Values up to between(400 - 500 mg/100 ml) are found in diabetes mellitus while in
jaundice increases occur most commonly when there is obstruction in the large bile ducts. The
increase roughly parallels the rise in serum bilirubin . Very high values are found in primary
biliary cirrhosis rising to (1000-2000 mg/100 ml) and somewhat smaller rises are associated
6. with drug - induced cholestasis . Values up to (500-700 mg/100 ml) are seen in myxoedema and
the determination may give , useful help in diagnosis . Smaller increases may be found in
hypopituitarism , usually in the range (250- 350 mg/100 ml) . Values between (300-400
mg/100ml) are rather frequent findings in coronary thrombosis and angina pectoris.
Atherosclerosis which refers to the deposition of fatty substances , largely but not entirely
cholesterol , in the walls of the arteries . Statistical studies have shown that the tendency to
develop atherosclerosis and coronary heart diseases is directly related to serum cholesterol
concentration which was increased in all heart diseases.
In pregnancy there is an increase in total cholesterol which may reach to 20-25 percent above
the normal at the 30th week.
Hypocholesterolemia :- Cholesterol is the plasma tend to fall during starvation and as the result
of prolonged debilitating illness. Hyperthyroidism (excess thyroid gland activity) also reduce
serum cholesterol, so that below (100 mg/l00ml) may be found in the severest cases, but this
change is not dose of diagnostic significance . Very low values occurs in abetalipoproteinemia
and to lesser extent in familial hypoabetalipoproteinemia in which the cholesterol level in
extremely low and can be regarded by neurological abnormalities and abnormalities in the
absorption of fats from the intestines. Free cholesterol normally forms about 30% of the total
(ranging from 20 - 40%). While changes in this in diabetes, the nephrotic syndrome and
myxoedema, the percentage of free cholesterol rise in liver disease The tendency when liver
cell damage is present , as in infective hepatitis, is that during the development of jaundice, the
total cholesterol either remains within normal limits or falls a little while the proportion of ester
7. falls appreciably and may reach very low levels . As the condition improves , the total
cholesterol are somewhat raised for a time after the jaundice has disappeared. In severe acute
hepatic necrosis , total cholesterol is usually low and may fall below (100 mg/100 ml) with
concomitant marked reduction in the ester proportion . In extra hepatic obstruction jaundice
although total cholesterol is increased, the ratio of free to ester change little . In primary biliary
cirrhosis the increase , however , is almost entirely in the free cholesterol which may as high as
90% .
Limitations
1.The reagent and sample volumes can be altered proportionately so that the sample:reagent,
ratio remains same.
2. Hemolytic and lipemic samples may result in false elevated results. To avoid false results
sample blank may be used as mentioned above: Add 10 l of serum sample to 1000 l of DI water
and read absorbance at 505nm. Subtract the absorbance obtained as above, from the
absorbance of test. this corrected absorbance is used for calculation. Reagents are sensitive to
light and temperature. Reagents may develop a slight pink color on ageing. This does not
interfere in the results. Quality Control The patient results obtained for each batch can be
validated by using normal and abnormal control sera with assayed values for cholesterol.
8. References:
1. Tietz NW. Clinical guide to laboratory tets, 2nd ed. Saunders Co., 1991.
2. Allain CC, Poon LS, Chan CSG, Richmond W and Fu PC. Enzymatic determination of total
serum cholesterol.Clin Chem 1978; 20: 470 – 475.
3.Daniel Steinberg (2007). The Cholesterol Wars: The Cholesterol Skeptics vs the
Preponderance of Evidence. Boston: Academic Press. ISBN 0- 12 373979-9
4- Varley . H. ; Gowenlock , A.M. and Bell, M. (1980) In "Practic Clinical Biochemistry " 5th ed.
Vol. I, WilliamHeinemann Medic Books LTD . , London .
5- Tietz , W.N. (1982) In "Fund, of Clinical Chemmistry " 2nd . ed| W.B. Saunders Co.,
Philadelphia .
6- Kaplan , L.A. and pesce , A.J. (1984) In " Clinical Chemistry " 1st , C.V. Mosby Co., St. Louis
7.Kenyatta university-laboratory-practical-manual- for-MBCHB-Year 1-semester1.