Bacteriocins are the extracellular proteins produced by the bacteria which inhibit the growth of similar or closely related bacterial strains. Lactic Acid Bacteria (LAB) are predominantly present in the fermented foods produce bacteriocins. In the present study, six strains (three strains each) were isolated from the fermented Bengal gram samples containing with husk and without husk.The organisms isolated were identified as Pediococcussp and Yeast sp.by the biochemical characterization. The bacteriocins produced by these organisms effectively inhibited the growth of E.coli, Klebsiellasp, Staphylococcus aureus, and Pseudomonas aerugenosabut couldn’t inhibit the growth of Proteus vulgaris and Bacillus sp. Strains c2 and c3 showed greater activity at low pH. All the strains showed antibacterial activity against Bacillus sp,E.coli, Klebsiellasp, Staphylococcus aureusand Pseudomonas aerugenosabut Proteus vulgaris showed high resistance when bacteriocin was exposed to temperatures 37°C and 80°C.
Isolation studies of Bacteriocin producing Lactic Acid Bacteria from raw goat...Premier Publishers
Probiotics are live microorganisms which when administered in appropriate amount gives health benefit on the host. In the current study raw goat milk obtained from Belapur village (Belapur, Navi Mumbai, Pin-400614, Maharashtra, India) was the source of isolated lactic acid bacteria. Out of 118 screened isolates, only one isolate i.e. RL 76 showed antimicrobial activity upon primary screening. Optimization of the isolate was carried out to check its bacteriocin producing potential. Isolate grew well at 370C and pH 7. Isolate RL 76 tolerated 2% of bile salt and showed no haemolytic activity. Upon morphological and biochemical tests, the isolate RL76 was identified as Lactobacillus casei. The extracted cell free supernatant was partially purified by ammonium sulphate precipitation. Partially purified cell free supernatant showed antibacterial activity against Staphylococcus aureus, Streptococcus fecalis, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli. The study indicates potential of the isolate as a good probiotic species.
Optimizing The Bacteriocin Production In Strain Of Lactobacillus pentosus Iso...Innspub Net
MRS agar was used for the isolation of Lactobacillus strain. This strain was recognized through biochemical tests and 16S rRNA ribotyping, as Lactobacillus pentosus. Its antibacterial effects were detected by utilizing cell free supernatant (CFS). Escherichia coli, Bacillus subtilis and Staphylococcus aureus were used as test strains. CFS showed antimicrobial activity against the test strains. CFS was treated with proteinases for the confirmation of loss of antimicrobial activity. Loss of antimicrobial activity on exposure to proteinases indicated the presence of bacteriocin in CFS. CFS was also studied for its antimicrobial effect at different temperatures and pH. Optimum antimicrobial effect was recorded at pH 7 and at temperature 45°C. The current study indicates the antimicrobial activity of strain of L. pentosus against E. coli, B. subtilis and S. aureus.
International Journal of Engineering and Science Invention (IJESI)inventionjournals
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Preservative potentials of crude bacteriocins produced by Lactobacillus tucce...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Isolation studies of Bacteriocin producing Lactic Acid Bacteria from raw goat...Premier Publishers
Probiotics are live microorganisms which when administered in appropriate amount gives health benefit on the host. In the current study raw goat milk obtained from Belapur village (Belapur, Navi Mumbai, Pin-400614, Maharashtra, India) was the source of isolated lactic acid bacteria. Out of 118 screened isolates, only one isolate i.e. RL 76 showed antimicrobial activity upon primary screening. Optimization of the isolate was carried out to check its bacteriocin producing potential. Isolate grew well at 370C and pH 7. Isolate RL 76 tolerated 2% of bile salt and showed no haemolytic activity. Upon morphological and biochemical tests, the isolate RL76 was identified as Lactobacillus casei. The extracted cell free supernatant was partially purified by ammonium sulphate precipitation. Partially purified cell free supernatant showed antibacterial activity against Staphylococcus aureus, Streptococcus fecalis, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli. The study indicates potential of the isolate as a good probiotic species.
Optimizing The Bacteriocin Production In Strain Of Lactobacillus pentosus Iso...Innspub Net
MRS agar was used for the isolation of Lactobacillus strain. This strain was recognized through biochemical tests and 16S rRNA ribotyping, as Lactobacillus pentosus. Its antibacterial effects were detected by utilizing cell free supernatant (CFS). Escherichia coli, Bacillus subtilis and Staphylococcus aureus were used as test strains. CFS showed antimicrobial activity against the test strains. CFS was treated with proteinases for the confirmation of loss of antimicrobial activity. Loss of antimicrobial activity on exposure to proteinases indicated the presence of bacteriocin in CFS. CFS was also studied for its antimicrobial effect at different temperatures and pH. Optimum antimicrobial effect was recorded at pH 7 and at temperature 45°C. The current study indicates the antimicrobial activity of strain of L. pentosus against E. coli, B. subtilis and S. aureus.
International Journal of Engineering and Science Invention (IJESI)inventionjournals
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Preservative potentials of crude bacteriocins produced by Lactobacillus tucce...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
An Investigation Into The Mechanisms Underlying Enhanced Biosulphidogenesis I...iosrjce
Anthropogenic activities like mining, processes of metallurgy and other chemical industries lead to
the discharge of a high amount of sulphate into the environment that causes serious problems to human health.
This paper illustrates the employment of thermophilic sulphate reducing bacteria for biosulphidogenesis. Two
different species have been isolated from hot water spring of Vajreshwari and Ganeshpuri,Thane, Maharashtra,
INDIA.The mechanism involved in biosulphidogenesis includes production of specific protein as well as
liberation of some extracellular polymeric compound (EPS) e.g. proteins, carbohydrate, acids etc. that are
produced during the microbial cell metabolism. These compounds plays an important role in the faster
reduction of sulphate and decrease in production rate of sulphide.The isolate was found to be of genus
Bacillusand type strain was found to be subtilis Zankar and licheniformis Sonali. The strain sequence were
deposited in NCBI database with accession number KJ939324 and KJ939325 respectively. The result highlights
the potential use of these organism in biosulphidogenesis.
Isolation and Identification High-Biological Activity Bacteria in Yogurt Qua...IJMER
Six strains of lactic bacteria and seven strains of acetic bacteria which were isolated from
fermented food. The screening secured one strain of lactic acid bacterium LB4 which carried strong
probiotic activity and one strain of acetic acid bacterium AB7 which was capable to generate high
glucuronic acid. Activities of probiotic and glucuronic acid were useful biological activities for health
protection and enhancing. The results of DNA sequencing showed LB4 was similar to Lactobacillus
acidophilus and AB7 was similar to Gluconacetobacter nataicola with 100% of similar proportion. Both
of these bacteria were safe that could be used in food fermentation.Six strains of lactic bacteria and seven strains of acetic bacteria which were isolated from
fermented food. The screening secured one strain of lactic acid bacterium LB4 which carried strong
probiotic activity and one strain of acetic acid bacterium AB7 which was capable to generate high
glucuronic acid. Activities of probiotic and glucuronic acid were useful biological activities for health
protection and enhancing. The results of DNA sequencing showed LB4 was similar to Lactobacillus
acidophilus and AB7 was similar to Gluconacetobacter nataicola with 100% of similar proportion. Both
of these bacteria were safe that could be used in food fermentation.
Anti-Microbial Activity of probiotic Lactobacilli and Optimization of Bacteri...ijsrd.com
The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.
Bioprocess development for enhanced spore production in shake flask and pilot...iosrjce
Bacillus thuringiensis subsp. Israelensis (Bti), has proven to be a safe and effective larvicide for controlling
mosquito and black fly larvae. The effect of cultivation and bioprocess development on Bti growth and sporulation was
investigated in shake flask level and batch cultivation in the semi-industrial scale 16-L stirred tank bioreactor. For
industrial production of biocontrol microorganism, it is necessary to obtain high cell mass and spore production in a short
time with low cost cultivation media. In this study, the new composition of production media was optimized which composed
of (g L
-1
): glucose, 10; yeast extract, 30; KH2PO4, 5; K2HPO4, 5; MgSO4. 7H20, 0.005; MnSO4.H2O, 0.03; FeSO4, 0.01;
CaCl2.7 H2O, 0.05; NaH2PO4, 1.5; NH4H2PO4, 1.5. The maximal cell dry mass and spore production, Sporemax for shake
flask study were 4.26 gL-1
at 36 h and 3.29106
spore mL-1
, respectively. Furthermore, studies of the cultivation conditions
under controlled and uncontrolled pH in the 16L-bioreactor was performed. The growth of Bti under uncontrolled pH
cultivation showing decreased of glucose and total protein concentration in the media was correlated with the vegetative cell
growth and sporulation. The maximal cell dry mass and Sporemax for uncontrolled pH bioreactor were 4.14 gL-1
at 36h and
3.7106
spore mL-1
, respectively. The maximal cell dry mass and spore production, Sporemax for controlled pH bioreactor
were 3.36 gL-1
at 26 h and 3.23 106
spore mL-1
, respectively. In conclusion, batch cultivation in 16-L bioreactor with the
new optimized production medium under uncontrolled pH condition increased of the cell dry mass and number of spores up to 23 % and 47 % , respectively
Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for various applications like dairy, pharmaceutical, and food etc. In this study, three types of pigments were isolated i.e. yellow from Xanthomonas sp., pinkish Red from Rhodotorula sp., and orange from Sarcina sp. Pigmented bacterial isolates were obtained from the soil samples and used for the pigment extraction study. We studied that the pigment producing bacteria and identified the color producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments were done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacterial sp. were used for the antimicrobial activity, antioxidant, and anticancer & transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and dying of cloth.
Key-words: - Soil samples, GC/MS analysis, UV-Visible spectrophotometry, Carotenoid, Pigment extraction
“Microbiological Analysis Of Soy Milk Produced From Soybean”iosrjce
The soy milk was prepared using the soaked soybean seeds then soy milk was treated with Na2CO3
and NaHCO3 for the preservation. By spread plate method and streak plate method two bacteria Escherichia
spp and Streptococcus spp were isolated respectively .These bacteria were subjected to biochemical and
microbiological analysis. In Biochemical analysis IMVIC test was performed. Both the bacteria Escherichia spp
and Streptococcus spp are positive for Methyl red test and citrate utilization whereas both the bacteria are
negative for Voges-Proskauer test and for Indole production test Escherichia spp is positive and Streptococcus
spp is negative. In case of microbiological test Escherichia spp is gram negative and Streptococcus spp is gram
positive. Both these bacteria have catalase production ability
Cell-free DNA Levels Serum Patients with Benign and Malignant Epithelial Ovar...inventionjournals
An elevated level of cell-free DNA (cfDNA) in the blood circulation has detected in cancer patients in comparison with healthy controls. CfDNA circulation in plasma and serum extensively studied and the results are highly variable due to many factors influence the test results that was preanalytic factors as well as analytic factors. Objectives: Is there any difference in the concentration of serum DNA among patients with benign epithelial ovarian tumors and malignant epithelial ovarian tumors? What is the clinicopathological variable that influences the cfDNA circulation? Method: Venous blood drawn with plain vacutainer, centrifuged at 1,000 rpm for 30 minutes, serum kept in -800 C freezer. The cfDNA extracted used NaI method.Results: Collected 30 cases of the benign ovarian tumor and 54 cases of malignant ovarian tumors. The average level serum cfDNA of benign epithelial ovarian tumors and malignant epithelial ovarian tumors were 24.6 ng/mL and 22:29 ng/mL respectively and statistically was not significantly different (p = 0.64). In multivariable analysis with linear regression, there were no clinicopathological variables that statistically significant influence the cfDNA levels in patients with epithelial ovarian tumors where p > 0.05. Conclusion: Concentration of cfDNA circulation of benign epithelial ovarian tumors a little bit higher than malignant epithelial ovarian tumors, but statistically was not significantly different. There was no clinicopathological variable influence the concentration of cfDNA circulation of ovarian tumors.
“Desquamative Gingivitis Treated By An Antioxidant Therapy- A Case Report”inventionjournals
Desquamative gingivitis is described as an erythematous, desquamated or eroded gingival lesion. Various etiologic factors are present for the appearance of such lesions. Despite of considering etiology, treatment is oftenly provided by systemic or topical corticosteroids. Apart from steroid application, another optionable treatment is antioxidant therapy which provides rapid healing of the tissue. As antioxidants posses various advantageous properties, it can be considered as a first treatment option for desquamative gingivitis. The presented case report of desquamative gingivitis is successfully treated using systemic antioxidants in the form of commercially available „oxitard capsule‟.
Analytical Studies for the Determination of Some Aromatic Amines with N-Bromo...inventionjournals
An accurate method has been described for determination of aromatic amines at micro scale using N-bromosaccharin as brominating agent in acetic acid medium. A known volume of sample solution of aromatic amines was treated with excess of N-bromosaccharin. After the reaction was completed the unreacted Nbromosaccharin was determined by titrating against standard sodium thiosulphate solution using starch as indicator. A blank Experiment was also run under identical condition without the sample. The method is simple quick, convenient and accurate and performed in an ordinary laboratory conditions without using any sophisticated instruments. The precision and accuracy are within + 2% .
Clinical and Laboratory Prognostic Factors in Malignant form of Mediterranean...inventionjournals
Mediterranean spotted fever (MSF) caused by Rickettsia conorii has become a significant health risk for suffering people and international travelers. In the past, overlooked as a serious disease, at present it is known that MSF was wrongly considered a benign condition. In this report, we present a set of clinical features and laboratory parameters in 55 patients (19 fatalities and 36 survivors) with malignant forms of the disease. The purpose of the study was to outline the prognostic factors of the fatal outcome in patients with malignant MSF. Based on our data, the main prediction factors for mortality in malignant MSF patients were: advanced age, delayed hospital admission, severe concomitant diseases, and failure to start or to complete appropriate antibiotic treatment. Laboratory prognostic factors in fatalities were: leukocytosis with a marked shift to the left; extremely high serum urea and creatinine levels; low levels of fibrinogen and prolongation of thrombin time. The most frequently involved organ systems of malignant cases were: central nervous system 100%, liver 92.72%, kidneys 60%, lungs 58.18%, myocardium 30.9%, and gastrointestinal tract 23.63%. The conducted histopathological investigations revealed lethal complications: encephalitis, brain edema, acute respiratory distress syndrome, non-cardiogenic lung swelling, acute myocarditis, gastrointestinal bleeding, hemorrhagicnecrotizing pancreatitis and acute renal failure.
ASSESSMENT OF NANOPARTICULATED RESVERATROL AND LOSARTAN IN THE PROPHYLAXIS OF...inventionjournals
The study demonstrated that eight weeks after bilateral ovariectomy in female white Wistar rats, develops endothelial dysfunction of bone microvasculature and deterioration of regional blood flow in the bone, leading to the emergence of generalized osteoporosis. Nanoparticulated forms of Losartan and Nanoparticulated forms of Resveratrol, possessing endothelial protective action, effectively prevent the reduction of regional microcirculation in bone tissue, keeping it at the level of intact rats. It is allowed to maintain an adequate level of bone remodeling processes, which manifested by slowing the thinning of bone trabeculae.
Assessment of Microbial Contamination of the Tooth Brush Head Used On Orthodo...inventionjournals
Introduction: Oral diseases can be greatly controlled by reducing the microbial load in the oral cavity and this can be achieved by maintaining proper oral hygiene.Tooth brushes are the most commonly used oral hygiene aid to promote oral health and prevent dental diseases. The insertion of fixed appliances alters the oral microbiological profile, thus increasing the risk for caries and gingivitis considerably. Aim: To assess the microbial growth of S.Mutans and Lactobacillus between and among the brushes. Setting and Study Design: A Hospital setting and Randomized Control study design Methods:A total of 56 (MB) patients aged 16-26 years received a toothbrush [Regular soft bristle design (group-A) and Orthodontic bristle design (group B)],A sterile gamma radiated pouch and checklist was distributed to each participant. After 2 weeks period the brushes were collected and placed in 5ml saline solution (0.05g Sodium Chloride). The suspension was incubated on selective agar plates and the amount of Streptococcus mutans and lactobacilli for each brush head was assessed. Results:The retention of S.Mutanswas found to be higher in group A, as compared to group B and was found to be statistically more significant between the two groups (P<0.001). The retention of Lactobacillus was also found to be higher in group A, as compared to group B and was found to be statistically significant between the groups (P= 0.001). However, there was no significant difference (P= 0.101) observedamong the microbial growth of S.Mutans and Lactobacillus in two bristle designs. Conclusions: Regular soft bristle design had a higher microbial load than those of subjects using orthodontic bristle design, a more frequent replacement of toothbrushes during t treatment may be advisable. Due to significant differences between the two bristle designs, the orthodontic toothbrush is recommended for patients undergoing orthodontic t appliances
An Investigation Into The Mechanisms Underlying Enhanced Biosulphidogenesis I...iosrjce
Anthropogenic activities like mining, processes of metallurgy and other chemical industries lead to
the discharge of a high amount of sulphate into the environment that causes serious problems to human health.
This paper illustrates the employment of thermophilic sulphate reducing bacteria for biosulphidogenesis. Two
different species have been isolated from hot water spring of Vajreshwari and Ganeshpuri,Thane, Maharashtra,
INDIA.The mechanism involved in biosulphidogenesis includes production of specific protein as well as
liberation of some extracellular polymeric compound (EPS) e.g. proteins, carbohydrate, acids etc. that are
produced during the microbial cell metabolism. These compounds plays an important role in the faster
reduction of sulphate and decrease in production rate of sulphide.The isolate was found to be of genus
Bacillusand type strain was found to be subtilis Zankar and licheniformis Sonali. The strain sequence were
deposited in NCBI database with accession number KJ939324 and KJ939325 respectively. The result highlights
the potential use of these organism in biosulphidogenesis.
Isolation and Identification High-Biological Activity Bacteria in Yogurt Qua...IJMER
Six strains of lactic bacteria and seven strains of acetic bacteria which were isolated from
fermented food. The screening secured one strain of lactic acid bacterium LB4 which carried strong
probiotic activity and one strain of acetic acid bacterium AB7 which was capable to generate high
glucuronic acid. Activities of probiotic and glucuronic acid were useful biological activities for health
protection and enhancing. The results of DNA sequencing showed LB4 was similar to Lactobacillus
acidophilus and AB7 was similar to Gluconacetobacter nataicola with 100% of similar proportion. Both
of these bacteria were safe that could be used in food fermentation.Six strains of lactic bacteria and seven strains of acetic bacteria which were isolated from
fermented food. The screening secured one strain of lactic acid bacterium LB4 which carried strong
probiotic activity and one strain of acetic acid bacterium AB7 which was capable to generate high
glucuronic acid. Activities of probiotic and glucuronic acid were useful biological activities for health
protection and enhancing. The results of DNA sequencing showed LB4 was similar to Lactobacillus
acidophilus and AB7 was similar to Gluconacetobacter nataicola with 100% of similar proportion. Both
of these bacteria were safe that could be used in food fermentation.
Anti-Microbial Activity of probiotic Lactobacilli and Optimization of Bacteri...ijsrd.com
The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.The present study is about the anti-microbial activity of the bacteriocin producing lactobacilli and optimization of bacteriocin production. Bacteriocin was extracted by solvent extraction with chloroform and the antimicrobial activity was tested against 5 different pathogens by agar spotting method. Optimization of bacteriocin production was done for 4 different parameters such as pH, Temperature, Carbon source and Nitrogen source and the anti-microbial activity was tested against the following 5 different pathogens and the results were observed and diameter of the zone of inhibition was measured and tabulated. From the results of the study it was found that bacteriocin produced from lactobacilli has good antimicrobial activity.
Bioprocess development for enhanced spore production in shake flask and pilot...iosrjce
Bacillus thuringiensis subsp. Israelensis (Bti), has proven to be a safe and effective larvicide for controlling
mosquito and black fly larvae. The effect of cultivation and bioprocess development on Bti growth and sporulation was
investigated in shake flask level and batch cultivation in the semi-industrial scale 16-L stirred tank bioreactor. For
industrial production of biocontrol microorganism, it is necessary to obtain high cell mass and spore production in a short
time with low cost cultivation media. In this study, the new composition of production media was optimized which composed
of (g L
-1
): glucose, 10; yeast extract, 30; KH2PO4, 5; K2HPO4, 5; MgSO4. 7H20, 0.005; MnSO4.H2O, 0.03; FeSO4, 0.01;
CaCl2.7 H2O, 0.05; NaH2PO4, 1.5; NH4H2PO4, 1.5. The maximal cell dry mass and spore production, Sporemax for shake
flask study were 4.26 gL-1
at 36 h and 3.29106
spore mL-1
, respectively. Furthermore, studies of the cultivation conditions
under controlled and uncontrolled pH in the 16L-bioreactor was performed. The growth of Bti under uncontrolled pH
cultivation showing decreased of glucose and total protein concentration in the media was correlated with the vegetative cell
growth and sporulation. The maximal cell dry mass and Sporemax for uncontrolled pH bioreactor were 4.14 gL-1
at 36h and
3.7106
spore mL-1
, respectively. The maximal cell dry mass and spore production, Sporemax for controlled pH bioreactor
were 3.36 gL-1
at 26 h and 3.23 106
spore mL-1
, respectively. In conclusion, batch cultivation in 16-L bioreactor with the
new optimized production medium under uncontrolled pH condition increased of the cell dry mass and number of spores up to 23 % and 47 % , respectively
Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for various applications like dairy, pharmaceutical, and food etc. In this study, three types of pigments were isolated i.e. yellow from Xanthomonas sp., pinkish Red from Rhodotorula sp., and orange from Sarcina sp. Pigmented bacterial isolates were obtained from the soil samples and used for the pigment extraction study. We studied that the pigment producing bacteria and identified the color producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments were done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacterial sp. were used for the antimicrobial activity, antioxidant, and anticancer & transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and dying of cloth.
Key-words: - Soil samples, GC/MS analysis, UV-Visible spectrophotometry, Carotenoid, Pigment extraction
“Microbiological Analysis Of Soy Milk Produced From Soybean”iosrjce
The soy milk was prepared using the soaked soybean seeds then soy milk was treated with Na2CO3
and NaHCO3 for the preservation. By spread plate method and streak plate method two bacteria Escherichia
spp and Streptococcus spp were isolated respectively .These bacteria were subjected to biochemical and
microbiological analysis. In Biochemical analysis IMVIC test was performed. Both the bacteria Escherichia spp
and Streptococcus spp are positive for Methyl red test and citrate utilization whereas both the bacteria are
negative for Voges-Proskauer test and for Indole production test Escherichia spp is positive and Streptococcus
spp is negative. In case of microbiological test Escherichia spp is gram negative and Streptococcus spp is gram
positive. Both these bacteria have catalase production ability
Cell-free DNA Levels Serum Patients with Benign and Malignant Epithelial Ovar...inventionjournals
An elevated level of cell-free DNA (cfDNA) in the blood circulation has detected in cancer patients in comparison with healthy controls. CfDNA circulation in plasma and serum extensively studied and the results are highly variable due to many factors influence the test results that was preanalytic factors as well as analytic factors. Objectives: Is there any difference in the concentration of serum DNA among patients with benign epithelial ovarian tumors and malignant epithelial ovarian tumors? What is the clinicopathological variable that influences the cfDNA circulation? Method: Venous blood drawn with plain vacutainer, centrifuged at 1,000 rpm for 30 minutes, serum kept in -800 C freezer. The cfDNA extracted used NaI method.Results: Collected 30 cases of the benign ovarian tumor and 54 cases of malignant ovarian tumors. The average level serum cfDNA of benign epithelial ovarian tumors and malignant epithelial ovarian tumors were 24.6 ng/mL and 22:29 ng/mL respectively and statistically was not significantly different (p = 0.64). In multivariable analysis with linear regression, there were no clinicopathological variables that statistically significant influence the cfDNA levels in patients with epithelial ovarian tumors where p > 0.05. Conclusion: Concentration of cfDNA circulation of benign epithelial ovarian tumors a little bit higher than malignant epithelial ovarian tumors, but statistically was not significantly different. There was no clinicopathological variable influence the concentration of cfDNA circulation of ovarian tumors.
“Desquamative Gingivitis Treated By An Antioxidant Therapy- A Case Report”inventionjournals
Desquamative gingivitis is described as an erythematous, desquamated or eroded gingival lesion. Various etiologic factors are present for the appearance of such lesions. Despite of considering etiology, treatment is oftenly provided by systemic or topical corticosteroids. Apart from steroid application, another optionable treatment is antioxidant therapy which provides rapid healing of the tissue. As antioxidants posses various advantageous properties, it can be considered as a first treatment option for desquamative gingivitis. The presented case report of desquamative gingivitis is successfully treated using systemic antioxidants in the form of commercially available „oxitard capsule‟.
Analytical Studies for the Determination of Some Aromatic Amines with N-Bromo...inventionjournals
An accurate method has been described for determination of aromatic amines at micro scale using N-bromosaccharin as brominating agent in acetic acid medium. A known volume of sample solution of aromatic amines was treated with excess of N-bromosaccharin. After the reaction was completed the unreacted Nbromosaccharin was determined by titrating against standard sodium thiosulphate solution using starch as indicator. A blank Experiment was also run under identical condition without the sample. The method is simple quick, convenient and accurate and performed in an ordinary laboratory conditions without using any sophisticated instruments. The precision and accuracy are within + 2% .
Clinical and Laboratory Prognostic Factors in Malignant form of Mediterranean...inventionjournals
Mediterranean spotted fever (MSF) caused by Rickettsia conorii has become a significant health risk for suffering people and international travelers. In the past, overlooked as a serious disease, at present it is known that MSF was wrongly considered a benign condition. In this report, we present a set of clinical features and laboratory parameters in 55 patients (19 fatalities and 36 survivors) with malignant forms of the disease. The purpose of the study was to outline the prognostic factors of the fatal outcome in patients with malignant MSF. Based on our data, the main prediction factors for mortality in malignant MSF patients were: advanced age, delayed hospital admission, severe concomitant diseases, and failure to start or to complete appropriate antibiotic treatment. Laboratory prognostic factors in fatalities were: leukocytosis with a marked shift to the left; extremely high serum urea and creatinine levels; low levels of fibrinogen and prolongation of thrombin time. The most frequently involved organ systems of malignant cases were: central nervous system 100%, liver 92.72%, kidneys 60%, lungs 58.18%, myocardium 30.9%, and gastrointestinal tract 23.63%. The conducted histopathological investigations revealed lethal complications: encephalitis, brain edema, acute respiratory distress syndrome, non-cardiogenic lung swelling, acute myocarditis, gastrointestinal bleeding, hemorrhagicnecrotizing pancreatitis and acute renal failure.
ASSESSMENT OF NANOPARTICULATED RESVERATROL AND LOSARTAN IN THE PROPHYLAXIS OF...inventionjournals
The study demonstrated that eight weeks after bilateral ovariectomy in female white Wistar rats, develops endothelial dysfunction of bone microvasculature and deterioration of regional blood flow in the bone, leading to the emergence of generalized osteoporosis. Nanoparticulated forms of Losartan and Nanoparticulated forms of Resveratrol, possessing endothelial protective action, effectively prevent the reduction of regional microcirculation in bone tissue, keeping it at the level of intact rats. It is allowed to maintain an adequate level of bone remodeling processes, which manifested by slowing the thinning of bone trabeculae.
Assessment of Microbial Contamination of the Tooth Brush Head Used On Orthodo...inventionjournals
Introduction: Oral diseases can be greatly controlled by reducing the microbial load in the oral cavity and this can be achieved by maintaining proper oral hygiene.Tooth brushes are the most commonly used oral hygiene aid to promote oral health and prevent dental diseases. The insertion of fixed appliances alters the oral microbiological profile, thus increasing the risk for caries and gingivitis considerably. Aim: To assess the microbial growth of S.Mutans and Lactobacillus between and among the brushes. Setting and Study Design: A Hospital setting and Randomized Control study design Methods:A total of 56 (MB) patients aged 16-26 years received a toothbrush [Regular soft bristle design (group-A) and Orthodontic bristle design (group B)],A sterile gamma radiated pouch and checklist was distributed to each participant. After 2 weeks period the brushes were collected and placed in 5ml saline solution (0.05g Sodium Chloride). The suspension was incubated on selective agar plates and the amount of Streptococcus mutans and lactobacilli for each brush head was assessed. Results:The retention of S.Mutanswas found to be higher in group A, as compared to group B and was found to be statistically more significant between the two groups (P<0.001). The retention of Lactobacillus was also found to be higher in group A, as compared to group B and was found to be statistically significant between the groups (P= 0.001). However, there was no significant difference (P= 0.101) observedamong the microbial growth of S.Mutans and Lactobacillus in two bristle designs. Conclusions: Regular soft bristle design had a higher microbial load than those of subjects using orthodontic bristle design, a more frequent replacement of toothbrushes during t treatment may be advisable. Due to significant differences between the two bristle designs, the orthodontic toothbrush is recommended for patients undergoing orthodontic t appliances
Study of the Thermal Behavior of the Plaster Reinforced Vegetable Fiberinventionjournals
This study focused on the characterization of a heat insulating material, aims to determine the thermal characteristics of the plaster reinforced with vegetable fibers. It intends to replace the fibers of sisal generally used in practice to strengthen the plaster is replaced by those of the RheckthophyllumCamerunense (RC), a fiber of humid equatorial forests, in applications such as the manufacture of the staff, separating walls of dwellings. The objective of this work is to make a comparative assessment of the thermal diffusivity using a material composed of high porous plaster, non-reinforced and strengthened with the new plant fiber. It also aims to study the influence of the architecture of the RC fibers. The testing of thermal transfer by infrared thermography without contact are conducted on the composite plates. The resolution of the heat equation by finite difference with Matlab program permit the identification of the thermal diffusivity by fitting the theoretical thermogramme representation on the measures curve. The results reveal that the vegetable fibers increase the thermal performance of the plaster; that the RC fibers favor the isolation function better than the fibers of sisal and that their random distribution in the material proved to be more favorable.
The Combined Effects of Omega3 Fatty Acids and NanoCurcumin Supplementation o...inventionjournals
Migraine is a chronic nerves system disease leads to considerable disabilities and affected quality of life. Neuro-inflammation play a key role in progression of migraine which mainly caused by release of inflammatory mediators including COX-2 and iNOs enzymes, TNF-α،IL-1β،IL-6 and adhesion molecule such as ICAM and VCAM.Curcumin and omega 3 fatty acids with anti-inflammatory and neuro-protective effects can reduce gene expression and production of inflammatory mediators. The combination of omega-3 and curcumin have also synergistic effects which a lower dose can induce a positive effect. The aim of present study is to determine whether omega 3 fatty acids, curcumin or combined of them as a complement treatment in migraine is helpful and the reduction gene expression and serum levels of inflammatory markers.The study will be conducted involving 80 episodic migraine patients that withstratified randomization method based on sex, gender and body mass index (BMI) are classified into 4 groups: 1) receiving omega 3 fatty acid supplement (1.8 gr/ day) and nano-curcumin supplement (80 mg/ day) 2) receiving omega 3 fatty acid supplement and curcumin placebo 3) receiving curcumin supplement and omega 3 fatty acid placebo 4) receiving omega 3 fatty acid placebo and curcumin placebo for 2 mounts. Blood samples will be collected after anthropometric parameter measuring then target biochemical parameters, gene expression and serum levels oftarget inflammatory mediators including COX-2 and iNOs enzymes, TNF-α،IL-1β،IL-6 and adhesion molecule such as ICAM and VCAM will be measured before and after the trial. The data will be statistically evaluated using the most appropriate tests.The results of current study will determine the efficacy of therapeutic effects of curcumin, omega 3 fatty acids and combined of them as a new insight to control and treatment of migraine.
Different Approaches of Software Requirement Prioritizationinventionjournals
In software system development, it can be a challenge for people to select the ‘right’ requirement among several or many options if it is not obvious which requirement is desirable. Requirements prioritization helps people to discover the most desirable requirements. It seems that most requirements prioritization techniques work well on a small number of requirements, but many of them have constraints on medium to large numbers of requirements. Requirement prioritization process is used to determine which candidate requirement of a software project should be included in a certain release, for this purpose different techniques are used. These techniques use different approaches and consider different factors for prioritization e.g. cost, value, risk, benefit etc.
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
Penicillin is drug of Choice for Syphilis- Still it holds good?inventionjournals
Syphilis resistant to Tetracyclins and macrolids had well documented. Penicillin is still considered as drug of choice in the treatment of Syphilis. Treponemes resistant to Penicillin are yet to be documented. In a private STD clinic of Tirunelveli, South India, during the period of January 2010 to June 2015, 68 cases reactive for syphilis were noted. 13.2 % of false positive were identified. Remaining 59 cases, 31 lost for follow up after treatment. Among whom 12 responded well to treatment following anti-syphilitic treatment with Penicillin whereas 16 (57.14%) remained reactive for more than a year or more. The titers either persist as same or even go up. Nobody in this group are reactive for HIV. All these patients remain in latent state in this series. Nobody progressed to neuro or cardiovascular syphilis. This warrants that it is high time to look for some other better alternative to treat Syphilis or otherwise increase in the dosage may have to be considered. Even though this study comprises of small number of patients, it has to be studied in detail for a longer period at multiple centers.
The Combined Effects of Omega3 Fatty Acids and NanoCurcumin Supplementation o...inventionjournals
Migraine is a chronic nerves system disease leads to considerable disabilities and affected quality of life. Neuro-inflammation play a key role in progression of migraine which mainly caused by release of inflammatory mediators including COX-2 and iNOs enzymes, TNF-α،IL-1β،IL-6 and adhesion molecule such as ICAM and VCAM.Curcumin and omega 3 fatty acids with anti-inflammatory and neuro-protective effects can reduce gene expression and production of inflammatory mediators. The combination of omega-3 and curcumin have also synergistic effects which a lower dose can induce a positive effect. The aim of present study is to determine whether omega 3 fatty acids, curcumin or combined of them as a complement treatment in migraine is helpful and the reduction gene expression and serum levels of inflammatory markers.The study will be conducted involving 80 episodic migraine patients that withstratified randomization method based on sex, gender and body mass index (BMI) are classified into 4 groups: 1) receiving omega 3 fatty acid supplement (1.8 gr/ day) and nano-curcumin supplement (80 mg/ day) 2) receiving omega 3 fatty acid supplement and curcumin placebo 3) receiving curcumin supplement and omega 3 fatty acid placebo 4) receiving omega 3 fatty acid placebo and curcumin placebo for 2 mounts. Blood samples will be collected after anthropometric parameter measuring then target biochemical parameters, gene expression and serum levels oftarget inflammatory mediators including COX-2 and iNOs enzymes, TNF-α،IL-1β،IL-6 and adhesion molecule such as ICAM and VCAM will be measured before and after the trial. The data will be statistically evaluated using the most appropriate tests.The results of current study will determine the efficacy of therapeutic effects of curcumin, omega 3 fatty acids and combined of them as a new insight to control and treatment of migraine.
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
Practical Guidelines to Improve Defect Prediction Model – A Reviewinventionjournals
Defect prediction models are used to pinpoint risky software modules and understand past pitfalls that lead to defective modules. The predictions and insights that are derived from defect prediction models may not be accurate and reliable if researchers do not consider the impact of experimental components (e.g., datasets, metrics, and classifiers) of defect prediction modeling. Therefore, a lack of awareness and practical guidelines from previous research can lead to invalid predictions and unreliable insights. Through case studies of systems that span both proprietary and open-source domains, find that (1) noise in defect datasets; (2) parameter settings of classification techniques; and (3) model validation techniques have a large impact on the predictions and insights of defect prediction models, suggesting that researchers should carefully select experimental components in order to produce more accurate and reliable defect prediction models.
Optimization of Bacillus Subtilis Natto Immobilization Process on Alginate – ...inventionjournals
Nattokinase is a potent fibrinolytic enzyme with the potential for fighting cardiovascular diseases. In this study, Bacillus subtilis natto were immobilized in the alginate – chitosan complex for fermentation of nattokinase enzyme. Six factors affecting the efficiency of immobilization cells were screened by Plackett – Burman design including: concentration of alginate, concentration of chitosan, pH of chitosan, concentration of CaCl2, added cells density, shaking time after supplementing chitosan. Results of optimization have identified two factors affecting the efficiency of cell immobilization. They are concentration of alginate (2.5%) and added cells density (approximately 5.86 million colonies per milliliter). With these two factors optimized and others kept at the normal level, immobilization efficiency reached 90.73%. After Bacillus subtilis natto had been immobilized by optimization of parameters, we conducted application for fermenting nattokinase. For 24 hours of fermentation, nattokinase enzyme activity reached 71.80 ± 0.19 FU/ml. Immobilized Bacillus subtilis natto cells were reused 6 times and on the 6 th time of reuse, nattokinase enzyme activity only decreased 2.7% in compared with the 1st reuse.
Correlations between Some Anthropometric Parameters, the Lipid Profile and Gl...inventionjournals
The study aimed to investigate the correlations between some anthropometric and lipid profile parameters, as well the glycated hemoglobin (HbA1c) values of Bulgarian females with type 2 Diabetes mellitus (T2DM). 212 women of Bulgarian ethnicity with T2DM of the age groups 40-60 and 61-80 years were included in the research. The anthropometric parameters: waist and hip circumferences were measured, and body mass index (BMI) and waist/hip ratio (WHR) were calculated. We also studied the lipid profile parameters: total cholesterol, triglycerides, high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL). A comparative study of the lipid profile criteria revealed significant differences in the levels of triglycerides, HDL-cholesterol and VLDL between the two age groups. Differences were found with regard to the values of glycated hemoglobin too. Correlations between the above anthropometric parameters and the lipid profile of patients with T2DM were examined. In patients of the age group 40-60 years a moderate strength correlation in opposite direction (negative or inverse correlation) was found between HbA1c and BMI (Pearson correlation coefficient 0.30-0.50, p<0.05). In the age group 61-80 years a low strength negative correlation was found between BMI and total cholesterol, as well as between BMI and LDL.
Cytogenetic, Hematological and Enzymes Levels Parameters in the Biomonitoring...inventionjournals
Studies have demonstrated genotoxic effects by the presence of micronucleus in exfoliated cells from the buccal mucosa of agricultural workers exposed to pesticides. This study has assessed the genotoxic effects of pesticides on 61 agricultural workers from the state of Piauí, Brazil. 31 individuals were exposed to pesticides and 30 are from the same area, but were not involved in pesticides application. Cytogenetic damage were evaluated through micronucleus test in cells from the buccal mucosa and some parameters such as hematological and levels of enzymes. Exposed individuals exhibited cytogenetic damage with increased number of micronuclei in cells from the buccal mucosa in comparison with subjects from the control group with significant statistical difference (P < 0.01). We perceive that there is a statistically no significant (P > 0.05) increase in levels of plasmatic and eritrocytaireacetylcholinesterase and no statistically significant increase of phosphatase alkaline were detected in exposed workers in relation to the control group. No association was found in relation to smoking habits, alcohol consumption, protection utensils and the biomarkers analyzed or the biochemical analysis. Analysis of variance revealed a correlation between occupational exposure to pesticides of workers in Piauí and the presence of micronuclei (P < 0.05).
Clinical and Laboratory Prognostic Factors in Malignant form of Mediterranean...inventionjournals
Mediterranean spotted fever (MSF) caused by Rickettsia conorii has become a significant health risk for suffering people and international travelers. In the past, overlooked as a serious disease, at present it is known that MSF was wrongly considered a benign condition. In this report, we present a set of clinical features and laboratory parameters in 55 patients (19 fatalities and 36 survivors) with malignant forms of the disease. The purpose of the study was to outline the prognostic factors of the fatal outcome in patients with malignant MSF. Based on our data, the main prediction factors for mortality in malignant MSF patients were: advanced age, delayed hospital admission, severe concomitant diseases, and failure to start or to complete appropriate antibiotic treatment. Laboratory prognostic factors in fatalities were: leukocytosis with a marked shift to the left; extremely high serum urea and creatinine levels; low levels of fibrinogen and prolongation of thrombin time. The most frequently involved organ systems of malignant cases were: central nervous system 100%, liver 92.72%, kidneys 60%, lungs 58.18%, myocardium 30.9%, and gastrointestinal tract 23.63%. The conducted histopathological investigations revealed lethal complications: encephalitis, brain edema, acute respiratory distress syndrome, non-cardiogenic lung swelling, acute myocarditis, gastrointestinal bleeding, hemorrhagicnecrotizing pancreatitis and acute renal failure.
Cytoprotective and DNA Protective Activity of Carica Papaya Leaf Extractsinventionjournals
Papaya (Carica papaya Linn) is commonly called as paw-paw and it belongs to the family Caricaceae. The properties of papaya fruit and other parts of the plant are also well known in traditional system of medicine. Papaya possess excellent medicinal properties for treatment of different ailments. These curative properties are based on the presence of phytochemical nutrients with antioxidant effect in different parts of the plant. It is considered as valuable nutraceutical fruit plant due to its biological activity and medicinal application.The present study was designed to determine the Cytoprotective and DNA protective activities of different fractions (Aqueous, Chloroform, Ethanol and Ethyl acetate extracts) of Carica papaya leaves. Cytoprotective capacity was assessed using erythrocytes, where ferrous sulphate was used to induce stress and the ability of the extracts to combat the induced stress was evaluated. The DNA protective potential against free radical-mediated oxidative stress was evaluated by a DNA damage inhibition assay involving agarose gel electrophoresis and UV spectrophotometric analysis. All the four fractions displayed significant cytoprotective effect on erythrocytes and prevented oxidative damage to DNA in presence of DNA damaging agent. Altogether, the results of our study lend pharmacological credence to the anti-cancerous and ethno medical use of this plant in traditional system of medicine and these resultscould be used to develop antimutagenic compounds for cancer therapy.
Existance Theory for First Order Nonlinear Random Dfferential Equartioninventionjournals
In this paper, the existence of a solution of nonlinear random differential equation of first order is proved under Caratheodory condition by using suitable fixed point theorem. 2000 Mathematics Subject Classification: 34F05, 47H10, 47H4
Revealing Biocontrol prospective of a few Bacterial Isolates segregated from ...IOSR Journals
In this paper an inquiry was conceded away for segregation of some microorganisms from rhizosphere zone of tea from tea growing regions of Barak valley, Assam; India. The isolates were secluded from tea rhizosphere of Toklai varieties (TV17, 20, 23) from some tea estates of Barak valley. Five isolates were recognized up to their genre stage on the foundation of their morphological and biochemical distinctiveness and finally the appraisal of disorganize possibility against fungi Fusarium oxysporum was ascertained and spelled out.
Isolation, Identification of Probiotic Bacteria Present in Milkijtsrd
The aim ofThe aim of this study was to present some data on isolation, growth, and antimicrobial activity, effect of pH, heat, and sensitivity to proteolytic enzymes of lactobacillus as probiotic bacteria. A large amount of probiotic bacteria is present in milk or milk products which are mainly lactic acid bacteria (LAB). Lactic acid bacteria (LAB) including Lactobacillus spp. Isolation, identification of lactic acid bacterial (LAB) was done by Gram' staining and catalase test and further confirmation was based on morphological, cultural, physiological and different biochemical tests. A total four isolates viz. Lactobacillus fermentum, L. casei, L. acidophilus and bifid bacterium longum was identified after different biochemical analysist which were also showed reliable probiotic properties. The antibacterial activity of lactic acid bacteria (LAB) isolated from raw milk against common enteric pathogens. The antagonistic properties of these isolates against Escherichia coli, Staphylococcus aureus, were examined using agar well diffusion method. this study was to present some data on isolation, growth, and antimicrobial activity, effect of pH, heat, and sensitivity to proteolytic enzymes of lactobacillus as probiotic bacteria. A large amount of probiotic bacteria is present in milk or milk products which are mainly lactic acid bacteria (LAB). Lactic acid bacteria (LAB) including Lactobacillus spp. Isolation, identification of lactic acid bacterial (LAB) was done by Gram' staining and catalase test and further confirmation was based on morphological, cultural, physiological and different biochemical tests. A total four isolates viz. Lactobacillus fermentum, L. casei, L. acidophilus and Bifidobacterium longum was identified after different biochemical analysist which were also showed reliable probiotic properties. The antibacterial activity of lactic acid bacteria (LAB) isolated from raw milk against common enteric pathogens. The antagonistic properties of these isolates against Escherichia coli, Staphylococcus aureus, were examined using agar well diffusion method. Shobha Mehra | Vimla Mehra | Dinesh Bhauryal"Isolation, Identification of Probiotic Bacteria Present in Milk" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd16979.pdf http://www.ijtsrd.com/biological-science/biotechnology/16979/isolation-identification-of-probiotic-bacteria-present-in-milk/shobha-mehra
Antimicrobial activity of Lactobacillus spp. especially (L. planetarium and L. acidophilus) against S. aureus were tested using agar-plug, agar well diffusion methods to select the best isolate that could inhibit the growth of multidrug resistance isolates. Further identification for the presence of bacteriocin was done using ELISA kit. Results showed that Lactobacillus spp isolates were bacteriocin producers with different degrees and that L. planetarium (L7) was the most efficient in bacteriocin production. Therefore, L. planetarium (L7) was selected for purification using 70% saturated ammonium sulfate and gel chromatography. The effect of purified bacteriocin was tested on 16 bacterial isolates using micro-titer plate method and well diffusion method. The results showed the ability of the bacteriocin to inhibit bacteria only at concentrations 1866U/ml (50%), 3732U/ml (100%) with a diameter of inhibition zones ranges between (11-23 mm) respectively. The anti-biofilm activity of purified bacteriocin at concentration 100% was investigated and the results showed that biofilm formation was reduced by 100% in the presence of bacteriocin.
Isolation and characterization of lipolytic Pseudomonas spp. from oil contami...iosrjce
Oil contaminated water samples collected from different areas in Pune were screened for the
selective isolation of lipolytic Pseudomonas spp. Screening medium containing Tributyrin (1% v/v) and
Rhodamine B was used for the specific isolation of lipolytic Pseudomonas spp. from oil contaminated water
samples. Lipolytic bacteria showing zone of clearance around the colonies were selected for the present study.
Isolated bacteria were identified upto genus level with the help of morphological and biochemical testing by
following Bergey’s manual. Spectrophotometric Lipase assay showed that out of 49 lipolytic Pseudomonas spp.,
WP23 exhibits the maximum Lipase activity of about 45.4733 U/ml.
Testing the ability against Bacillus cereus of actinobacteria strains isolate...Agriculture Journal IJOEAR
This study aimed to test the antibacterial activity of Bacillus cereus of actinobacterial isolates isolated from marine sponges in the Kien Giang Sea, Vietnam. That can select the strains with high resistance to identify them. There were 198 actinobacterial isolates tested. Based on the ability of antimicrobial activity to B. cereus, 82/198 had the against B. cereus, in which there were six isolates with high (7.3%), 52 medium (25.6%), and 21 weak resistance (67.1%). Selection of six isolates with the best resistance to B. cereus (ND1.7a, ND2.7c, HD1-3e, HD1-6a, HD2.3b, and H6b) identified by PCR and 16S rRNA gene sequencing. The results identified five strains of Streptomyces (Streptomyces tateyamensis ND1.7a, Streptomyces althioticus HD1.3e, Streptomyces flaveolus HD1.6a, Streptomyces olivaceus HD2.3d, and Streptomyces albidoflavus H6b) and one strain of genus Microbacterium (Microbacterium tumbae ND2.7c).
Studies on cellulose degrading microorganisms associated with rumen of rumina...Premier Publishers
Studies on cellulose degrading microorganisms associated with rumen of ruminants was carried out from ruminants (ram, cow, and goat), through culture, microscopic identification, Biochemical test and cellulose degrading methods. In the rumen content of ram four bacteria were isolated Bacteriodes and Staphylococcus had the highest percentage (33.3%) each while Veillonella and Bacillus had 16.6% each. Seven bacteria were isolated from cow with Streptococcus having (22.2%) Staphylococcus (22.2%), while Bacteroides, Yersinia, Peptococcus, Nesseria and Bacillus had equal distribution. Goat had eight bacteria including Bacteroides, Clostridium, Yersinia, Staphylococcus, Homofermentative Lactobacillus Alcaligens and Bacillus all of which had equal distribution. Bacteroides and Bacillus are common in all rumens, with Bacteroides, being more prevalent in the ram. study revealed that ruminants harbors various organisms that are active cellulose degraders, out of which Bacteroides specie grow best on cellulose agar. For fungi, Aspergillus flavus and Aspergillus fumigatus highly degrades cellulose, Scopulariopsis candida degrades minimally. The study revealed that ruminants harbors various organisms that are active cellulose degraders, out of which Bacteroides specie grow best on cellulose agar. Therefore, Rumen should be used as a site for isolation of micro organisms capable of cellulose hydrolysis in order to reduce the coast of purchasing commercial enzymes
Optimum Conditions for Alginaseby Bacilllus Circulans R Isolateiosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
ABSTRACT- Live microorganisms, have beneficial effects on their host’s health, are called as probiotics. There are various possible sources to isolate
these bacteria. In this studyp harmaceutical probiotic sachet is used as isolation source. The purpose of this study is to search the potentiality of
probiotic bacteria and investigate the probiotic properties of isolates.9 different samples of 3 brands of sachet were used for isolation of bacteria.
Isolates were examined according to their probiotic properties. The probiotic characteristics like pH and Bile tolerance, Antagonistic activity and
Antibiotic susceptibility of isolated bacteria Such as Lactobacillus acidophilus, Lactobacillus rhamnosus and Bifidobacterium bifidum was done. Bile
Tolerance and pH tolerance was determined with the help of the help of coefficient of growth inhibition if their coefficient of growth inhibition is less
than 0.5 the organism was considered as the pH and Bile tolerance. The Strains of Lactobacillus acidophilus and Lactobacillus rhamnosus and Bifidobacterium
bifidum show best result at the pH Acidic to Neutral (5 to 7) and show a bile tolerance from 1-4 % bile. All the isolated bacteria show
the maximum inhibition against Staphyloccocus aureus and minimum against Salmonella typhi by Lactobacillus Strains but Bifidobacterium show
minimum against Escheria coli. Most isolates show resistance toward antibiotics. From this study it can be concluded that pharmaceutical probiotic
products used in the study were showing satisfactory quality and potential probiotic strain.
Key words- Probiotic, Lactobacillus, Bifidobacterium, Sachet
Similar to Isolation and Characterization of Bacteriocin Producing Lactic Acid Bacteria from Fermented Bengal Gram (20)
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
CFD Simulation of By-pass Flow in a HRSG module by R&R Consult.pptxR&R Consult
CFD analysis is incredibly effective at solving mysteries and improving the performance of complex systems!
Here's a great example: At a large natural gas-fired power plant, where they use waste heat to generate steam and energy, they were puzzled that their boiler wasn't producing as much steam as expected.
R&R and Tetra Engineering Group Inc. were asked to solve the issue with reduced steam production.
An inspection had shown that a significant amount of hot flue gas was bypassing the boiler tubes, where the heat was supposed to be transferred.
R&R Consult conducted a CFD analysis, which revealed that 6.3% of the flue gas was bypassing the boiler tubes without transferring heat. The analysis also showed that the flue gas was instead being directed along the sides of the boiler and between the modules that were supposed to capture the heat. This was the cause of the reduced performance.
Based on our results, Tetra Engineering installed covering plates to reduce the bypass flow. This improved the boiler's performance and increased electricity production.
It is always satisfying when we can help solve complex challenges like this. Do your systems also need a check-up or optimization? Give us a call!
Work done in cooperation with James Malloy and David Moelling from Tetra Engineering.
More examples of our work https://www.r-r-consult.dk/en/cases-en/
Water scarcity is the lack of fresh water resources to meet the standard water demand. There are two type of water scarcity. One is physical. The other is economic water scarcity.
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Isolation and Characterization of Bacteriocin Producing Lactic Acid Bacteria from Fermented Bengal Gram
1. International Journal of Pharmaceutical Science Invention
ISSN (Online): 2319 – 6718, ISSN (Print): 2319 – 670X
www.ijpsi.org Volume 5 Issue 4 ‖ July 2016 ‖ PP. 41-46
www.ijpsi.org 41 | P a g e
Isolation and Characterization of Bacteriocin Producing Lactic
Acid Bacteria from Fermented Bengal Gram
B.Aruna1
, D. Mounica Srivalli2
1, 2
(Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad-500016)
ABSTRACT
Bacteriocins are the extracellular proteins produced by the bacteria which inhibit the growth of similar or
closely related bacterial strains. Lactic Acid Bacteria (LAB) are predominantly present in the fermented foods
produce bacteriocins. In the present study, six strains (three strains each) were isolated from the fermented
Bengal gram samples containing with husk and without husk.The organisms isolated were identified as
Pediococcussp and Yeast sp.by the biochemical characterization. The bacteriocins produced by these organisms
effectively inhibited the growth of E.coli, Klebsiellasp, Staphylococcus aureus, and Pseudomonas
aerugenosabut couldn’t inhibit the growth of Proteus vulgaris and Bacillus sp. Strains c2 and c3 showed
greater activity at low pH. All the strains showed antibacterial activity against Bacillus sp,E.coli, Klebsiellasp,
Staphylococcus aureusand Pseudomonas aerugenosabut Proteus vulgaris showed high resistance when
bacteriocin was exposed to temperatures 37°C and 80°C.
Keywords: Antibacterial Activity,Bacteriocin, Lactic Acid Bacteria.
I. Introduction
The Lactic acid bacteria (LAB) refer to a large group of beneficial bacteria that have similar properties and all
produce lactic acid as an end product of the fermentation process. Additional characteristic flavours and aromas
are often the result of other products of lactic acid bacteria. LAB prevents the growth of pathogenic bacteria in
different eco-systems by production of antimicrobial substance such as lactic acid, acetic acid, diacetyl, and
hydrogen peroxide and bacteriocins[1]. Bacteriocin in fermented foods, originally defined asproteinaceous
compounds [2,3]. For the first time bacteriocin were discovered by Gratia [4]. Bacteriocins are extracellulary
released peptides or protein molecules produced by LAB, with a bactericidal and bacteriostatic mode of action
against closely related species.
The antimicrobial activity of LAB plays an important role in the food industry, agriculture and pharmaceutical
industry. They are used as bio preservative agent [5]. The inhibitory spectrum of some bacteriocins includes
food spoilage and/or food-borne pathogenic microorganisms [6]. However, some of the bacteriocins produced
by Gram positive bacteria, which exhibit a much broader spectrum of antagonism. These bacteriocins may act
not only against related species, but also against unrelated genera [7]. Bacteriocins are typically considered to be
narrow on antibiotics [8]. Bacteriocins may act on cells in different ways to lyse the cell [9]. These compounds
cause leakage of ions and other cellular components, and disrupt the proton motive force which results in cell
death [10].The competitive removal of essential substrates, the accumulation of D-amino acids, a lowering of
oxidation-reduction potential and coaggregation may further restrict undesirable microorganisms. The present
study includes, isolation of LAB from fermented Bengal gram, their effect of bactericidal and biopreservative
properties.
II. Materials And Methods
2.1. Sample collection and preparation
Bengal gram with husk and without husk were collected and cleaned separately. They were soaked in water for
8hrs so that the grains get soften. The soaked grains was made into batter using electric blender. The two batters
were allowed to ferment at room temperature overnight.
2.2. Isolation of organism from fermented bengal gram
One gram of Bengal gram with husk and 1g of Bengal gram without husk were weighed and added into 100 ml
of distilled water. After homogenization, serial dilutions were performed up to 10-10
and plated on de Man
Rogosa and Sharpe (MRS) agar. Three samples of each with husk and without husk are taken. The plates were
incubated at 37°C for 24 h. The colonies were then sub cultured in MRS broth twice and plated on MRS agar for
its purity.
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2.3. Identification of organisms
The cultures were identified according to their morphological, cultural, physiological and biochemical
characteristics [11, 12]. The used tests were: Gram reaction; production of catalase, cytochrome oxidase and
hydrogen peroxide; acid production from carbohydrates. The HIMEDIA carbohydrate fermentation discs of
Fructose, Galactose, Lactose, Maltose, Mannitol and Sucrose were used. The tubes were filled with peptone
water, Durham tubes and Methylene Blue (indicator) was added to each tube. The tubes are inoculated with
isolated organisms and the respective carbohydrate discs were added, incubated at 37º C for 24 h and observed
for acid and gas production. Durham tubes are used to detect the production of gas by isolated organisms. The
colour change from blue to yellow indicates the production of acid by isolated organism.
2.4. Preparation of culture supernatants
As bacteriocins are extracellular released proteins, culture supernatants were tested for antibacterial activity. The
pure culture isolated from fermented Bengal gram were propagated in MRS broth and incubated at 37°C for
24h.Bacterial cells were separated by centrifugation at 10,000 rpm for 20 min. The supernatant of each sample
was collected in to different test tubes separately and is used for further tests.
2.5. Antibacterial activity of culture supernatants
The antibacterial activity of culture supernatants were tested against both gram positive and gram negative
organisms such as Bacillus sp, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli,
Klebsiellasp, Proteus vulgaris by agar well diffusion method.
2.6. Antibiotic sensitivity of isolated strains
The responses of isolated organism to commercially available antibiotic discs were observed on MRS agar at
37°C for 24h. The zone of inhibition (sensitivity) around the discs and absence (resistance) of zone around the
discs were noted down.
2.7. Characterization of partially purified bacteriocin
The bacteriocin produced by different strains wasstudied with respect to stability at different temperature and
pH.
2.7.1. Effect of heat treatment
The culture supernatant of 1 ml each was taken in eppendorf tube. The water bath was set according to the
required temperature using thermometer. The culture supernatant samples were exposed for 1 h, at 37°C and
80°C. The bacteriocin activity was tested after 20 min by agar well diffusion.
2.7.2. Effect of pH sensitivity
The sterile cell free supernatants were adjusted with sterile NaOH or HCl to pH values of 2 and 7, incubated at
room temperature for 4 hand analyzed for bacteriocin activity.
2.7.3. Effect of bacteriocin as biopreservative agent
The biopreservative property of bacteriocin of only one Isolate c2 was observed. The bacteriocin 200µl was
applied on the surface of fresh Tomato, Capsicum and Brinjal with the help of sterile cotton then left at room
temperature and observed for spoilage after seven days.
III. Result And Discussion
3.1. Isolated strains
The five isolated colonies from the fermented bengal gram on MRS media appeared circular, 2 mm in size,
creamish white in colour. The isolates c1, c2, c3 (with husk) and c/o1, c/o3 (without out husk) were Gram
positive cocci. The isolate c/o2 was spindle shaped and identified as Yeast species. The Isolates showed no
hemolysis on blood agar.
Table I: General properties of isolated strains
Sample General properties Identified organism
c1(Bengal Gram with husk) Gram positive cocci, Catalase -ve, Oxidase
-ve, No hemolysis
Pediococcus sp.
c2 (Bengal Gram with husk) Gram Positive Cocci, Catalase -ve, Oxidase
-ve, No hemolysis.
Pediococcus sp.
c3(Bengal Gram with husk) Gram Positive Cocci, Catalase -ve, Oxidase
-ve, No hemolysis.
Pediococcus sp.
c/o1(Bengal Gram without husk) Gram Positive Cocci, Catalase -ve, Oxidase
-ve, No hemolysis.
Pediococcus sp.
c/o2 (Bengal Gram without husk) Gram Positive, buddingtypecells Yeast sp.
c/o3 (Bengal Gram without husk) Gram Positive Cocci, Catalase -ve, Oxidase
-ve, No hemolysis.
Pediococcus sp.
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All the isolates showed negative results for catalase and oxidase tests. The isolates c1, c2, c3, c/o1 and c/o3
showed no growth on Mannitol salt agar (MSA) Based on Bergey’s Manual of Systematic Bacteriology the
strains isolated on the MRS plates were identifies as non-pathpgenicPediococcussp (Table I). The
characterization studies of the bacteriocin produced by the six isolated was carried out under aerobic conditions.
Screened and characterized 100 strains of bacteriocin producing lactic acid bacteria from traditional fermented
foods [19] [13]and Identified 16 lactic acid bacteria from raw and fermented products[14].
3.2. Carbohydrate fermentation
The Table II shows the variation in the sugar fermentation by isolated species. The fermentation of fructose,
maltose, mannitol and sucrose were observed by four isolates (c1, c2, c3 and c/o1). The Isolate c/o2 fermented
fructose, galactose and sucrose. Maltose and mannitol were fermented by four isolates (c2, c3, c/o1 and c/o2)
fermented sucrose. The mannitol was fermented by c3 and maltose was fermented by c/o1. The c1, c2, c3 (with
husk) fermented lactose whereas the c/o1, c/o2, c/o3 (without husk) were negative for lactose fermentation. The
isolate c/o 3 didn’t ferment any of the carbohydrates that were included in the experiment. The lactose was the
only carbohydrate that was not fermented by isolates of fermented bengalgram.
Table II: Carbohydrate fermentation test of isolates
Isolates Fructose Galactose Lactose Maltose Mannitol Sucrose
c1 +ve +ve -ve +ve +ve +ve
c2 +ve -ve -ve +ve +ve +ve
c3 +ve +ve -ve +ve +ve +ve
c/o1 +ve -ve -ve +ve +ve +ve
c/o3 -ve -ve -ve -ve -ve -ve
+ve: Positive (Presence of carbohydrate fermentation),
-ve: Negative (Absence of carbohydrate fermentation).
The bacteriocin producing LAB have attracted significant attention because of their GRAS status and potential
use as safe additives for food preservation [15].Bacteriocin producing LAB isolated from fermented foods are
one of the best substances for improving the microbiological safety of that particular food as that are adapted to
traditional conditions better than those isolated from other sources [16]. The isolates from the fermented Bengal
gram batter were tested for the ability to produce lactic acid (Table II). The LAB produces lactic acid as the
major end product of the fermentation of carbohydrates and they are the most important bacteria in desirable
food fermentations [17].
3.3. Antibacterial activity of bacteriocin against indicator organisms
Table III depicts the antibacterial activity of the isolated strains. The six indicator organisms used for testing
antagonistic property of bacteriocin were Bacillus subtilis, Escherichia coli, Klebsiella sp., Proteus vulgaris,
Pseudomonas aeruginosaand Staphylococcus aureus. In the present study, the highest inhibitory activity was
observed by bacteriocin of all the six isolates against Escherichia coli and no antagonistic activity against
Proteus vulgaris. The bacteriocin of isolate c/o1 isolate was not able to inhibit S.aureus at 37o
C but showed
antagonistic activity at 37o
C with all indicator strains. At temperature 80o
C bacteriocin of c/o1 isolate showed
antagonistic activity with all the strains. Inhibition of E.coli and Klebsiellasp. were observed at 37o
C whereas at
80o
C only Klebsiella sp. was inhibited not E.coli.
Table III: Effect of temperature on antibacterial activity of bacteriocins
The inhibitory effect demonstrated by isolated strains against indicator organisms indicates the property of
antibacterial activity. The MRS medium is better for the cell growth and bacteriocinproduction than any other
media [18]. Generally maximum production corresponds with maximum cell concentration. Bacteriocin
production by LAB occurs during active growth phase. The high cell yield does not necessarily result in
increased bacterioncin activity as several mechanisms can be responsible for the decrease of activity, such as
protein aggregation proteolytic degradation by specific and non-specific enzymes and bacteriocin adsorption to
producer cells [19].
Zone of inhibition (mm)
Organism B.subtilis E.coli Klebsiella sp. P.aeruginosa S.aureus P.vulgaris
Temperature (°C) 37 80 37 80 37 80 37 80 37 80 37 80
Isolates
c1 - 17 20 - 19 15 21 25 15 14 - -
c2 15 13 15 - 15 17 25 18 16 10 - -
c3 12 16 13 - 14 15 - 17 15 - - -
c/o1 10 18 20 20 16 13 21 25 - 16 - -
c/o3 - 15 16 - 16 18 20 22 15 - - -
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3.4. Antibacterial activity of bacteriocin of isolates and positive controls (Milk and Sporolac powder)
The antibacterial activity (Figure 1) could not be attributed to the production of organic acids and hydrogen
peroxide. By using MRS medium, which contains low glucose (0.2%) the carbon source was reduced so that the
LAB cannot produce organic acids to the level that affects the growth of the other bacteria.
Figure 1: Antibacterial activity of bacteriocinof isolates c1, c2, c3, c/o1, c/o3, M (Milk: Positive control), S
(Sporolac Powder: Positive control)
Under aerobic conditions LAB cannot produce hydrogen peroxide to inhibit the growth of indicator
organisms.The results indicate that all isolates showed antibacterial effect against Bacillus subtilis, Escherichia
coli, Klebsiella sp., Pseudomonas aeruginosaand Staphylococcus aureus(Picture not included), confirming the
inhibition was due to bacteriocin[20, 21]. The growth of Proteus vulgaris was not inhibited by bacteriocin of all
isolates, indicating its resistant nature towards bacteriocin (Picture not included).
3.5. Antagonistic activity of bacteriocin at pH 2 and pH 7
The Table IV depicts the antagonistic activity of the bacteriocin produced by the six isolates at pH 2 and 7. All
the bacteriocins were active and stable at pH 2 against B.subtilis, E. coli, Klebsiella sp., P. aeruginosa and
S.aureus and P.vulgaris. The maximum activity was observed in the bacteriocin produced by isolate c1 and the
least activity by isolate c/o1.
Table IV: Effect of pH on antibacterial activity of bacteriocin
Zone of inhibition (mm)
Organism B.subtilis E.coli Klebsiella sp. P.aeruginosa S.aureus P.vulgaris
pH 2 7 2 7 2 7 2 7 2 7 2 7
Isolates
c1 19 15 30 - 32 - 32 - 28 - 30 -
c2 20 20 20 25 29 30 29 25 20 - 20 -
c3 19 20 19 19 25 16 26 21 22 - 24 -
c/o1 15 30 25 - 24 - - - 18 - 19 -
c/o2 20 40 24 - 27 12 30 - 24 - 28 -
c/o3 15 14 25 - 24 - 34 - 30 - 29 -
The bacteriocin of isolates c2 and c3 inhibited the growth of all indicator organisms at pH 2 and pH 7, indicating
its stability at acidic and neutral pH.A study related to the pH dependent activity of bacteriocin produced by
LAB showed that the majority of strains have the highest microbial activity at pH range of 2.0 - 4.0 [22].
3.6. Antibiotic sensitivity of isolates
The isolated strains (Table V) were tested against commercially available antibiotic discs of Ampicillin,
Penicillin, Streptomycin and Tetracycline. The Isolates c1, c2, c3 and c/o1 were sensitive to against Ampicillin
and Tetracycline and resistant against Penicillin and Streptomycin. The isolates c/o2 and c/o3 were resistant
against to all four antibiotics.
Table V: Antibiotic sensitivity test of isolates against four antibiotics
Antibiotics
Isolate Ampicillin Penicillin Streptomycin Tetracycline
c1 +ve -ve -ve +ve
c2 +ve -ve -ve +ve
c3 +ve -ve -ve +ve
c/o1 +ve -ve -ve +ve
c/o2 -ve -ve -ve -ve
c/o3 -ve -ve -ve -ve
+ve: Positive (Sensitive); -ve: Negative (Resistant)
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The performance of antimicrobial susceptibility testing by the clinical microbiology laboratory is important to
confirm susceptibility to chosen empirical antimicrobial agents, or to detect resistance in individual bacterial
isolates. Susceptibility testing of individual isolates is important with species that may possess acquired
resistance mechanisms [23].
3.7. Bio preservative property of bacteriocin
The partially purified bacteriocin from only one isolate c2 was tested for preservative effect. The bacteriocin of
isolate c2 applied topically on the surface of Tomato, Capsicum, Brinjaland observed for a period of one week.
The samples without the application of bacteriocin got spoiled within a period of 2-3 days whereas the samples
with the bacteriocin application were still fresh for seven days. This experiment revealed that the bacteriocins
application prevented the growth of spoilage bacteria, indicating the biopreservative property of bacteriocin. A
potentially novel pediocin NV5 was found active against some species of Enterococcus, Leconostoc,
Staphylococcus, many of which are associated with food spoilage and food related health hazards [24].
Figure 2: Bio preservative property of bacteriocin of isolate c2
Brinjal 1, Capsicum 1, Tomato 1: No topical application of bacteriocin (Spoiled).
Brinjal2, Capsicum 2, Tomato 2: Topical application of bacteriocin (Healthy).
IV. Conclusion
Inconclusion Lacticacidbacteria isolates from fermented Bengal gram batter had good antimicrobial activity
against foodborne pathogens. The bacteriocin of two isolates was active at pH 2 and pH 7. The topical
application of bacteriocin of isolate c2 showed preservative effect. These studies highlight the possibility that
these bacteriocins and LAB will be notified of great interest in terms of antimicrobial compounds in further
research.
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