Cleanflight is an open source flight controller software used on 32-bit flight controllers for multirotor aircraft and fixed-wing aircraft. It provides tuning and configuration options through tabs like setup, ports, configuration, PID tuning, receiver, modes, adjustments and more. Basic setup involves flashing a cleanflight-enabled flight controller board like the Naze32 via USB, then configuring options in the tabs like PID settings, receiver type, and motor directions. The command line interface (CLI) allows changing settings like looptime and filters to optimize performance.