This document discusses various inline sample preparation techniques for ion chromatography using Metrohm equipment, including ultrafiltration, stop flow dialysis, preconcentration, matrix elimination, and matrix neutralization. It provides examples of applying these techniques to samples such as vegetable juice, milk, cutting oil emulsions, water, and caustic soda to remove particles, concentrate analytes, and eliminate or neutralize matrix interferences for improved chromatographic analysis.
ChemFET fabrication, device physics and sensing mechanismRichard Yang
Ph.D. thesis work at UCSD. Developed ChemFET from Si substrate to functioning device, bluetooth enabled handheld package. The integrated nanosensor concept 10yrs could come to live on wearable electronics train.
The slickwater stimulation of unconventional gas and oil shale plays creates flowback water with a composition that is unique to particular shale formations. Characteristically, these fluids contain high concentrations of salts (e.g., chloride, bromide) which are routinely determined using ion chromatography. This analysis typically requires sample preparation, including manual dilution, which can significantly increase the cost of analysis. Results presented will show highly reproducible determination of anions and cations from Marcellus Shale flowback water using inline conductivity to identify high salt samples and then automatically diluting them prior to injection, saving time and column life.
Determination of Common Counterions and Impurity Anions in Pharmaceuticals Using a Capillary HPIC System with Suppressed Conductivity and Charge Detection
Recently, identification and quantification of ions in early stage drug development has gained increasing attention, because the APIs maybe contaminated with different counter ions from synthesis steps, and because selecting the counter ion to enhance APIs’ solubility and stability is becoming a key step in formulation development. This presentation demonstrates the identification and quantification of 22 commonly found anions in pharmaceuticals in a single run using a high-pressure capillary IC system (HPIC) with 4-μm particle ion –exchange column, and CD-QD dual detectors.
Liquiline system ca80 no endress+hauser datasheet-colorimetric analyzer for n...ENVIMART
Liquiline system ca80 no endress+hauser datasheet-colorimetric analyzer for nitrite - Endress+Hauser - Envimart JSC - www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường.
ChemFET fabrication, device physics and sensing mechanismRichard Yang
Ph.D. thesis work at UCSD. Developed ChemFET from Si substrate to functioning device, bluetooth enabled handheld package. The integrated nanosensor concept 10yrs could come to live on wearable electronics train.
The slickwater stimulation of unconventional gas and oil shale plays creates flowback water with a composition that is unique to particular shale formations. Characteristically, these fluids contain high concentrations of salts (e.g., chloride, bromide) which are routinely determined using ion chromatography. This analysis typically requires sample preparation, including manual dilution, which can significantly increase the cost of analysis. Results presented will show highly reproducible determination of anions and cations from Marcellus Shale flowback water using inline conductivity to identify high salt samples and then automatically diluting them prior to injection, saving time and column life.
Determination of Common Counterions and Impurity Anions in Pharmaceuticals Using a Capillary HPIC System with Suppressed Conductivity and Charge Detection
Recently, identification and quantification of ions in early stage drug development has gained increasing attention, because the APIs maybe contaminated with different counter ions from synthesis steps, and because selecting the counter ion to enhance APIs’ solubility and stability is becoming a key step in formulation development. This presentation demonstrates the identification and quantification of 22 commonly found anions in pharmaceuticals in a single run using a high-pressure capillary IC system (HPIC) with 4-μm particle ion –exchange column, and CD-QD dual detectors.
Liquiline system ca80 no endress+hauser datasheet-colorimetric analyzer for n...ENVIMART
Liquiline system ca80 no endress+hauser datasheet-colorimetric analyzer for nitrite - Endress+Hauser - Envimart JSC - www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường.
Analysis of Cations in Hydraulic Fracturing Flowback Water from the Marcellus Shale Using Ion Chromatography
This presentation describes the determination of cations in hydraulic fracturing flowback water using ion chromatography. In this work, sodium was most abundant, followed by calcium, strontium, magnesium, potassium, barium, ammonium, and then lithium, respectively. The quantity of scale-forming ions, such as calcium, strontium, and barium, is particularly informative because it can be used to determine the amount of anti-scaling agent in fracturing fluid mix that will maximize hydrocarbon recovery.
Design of a Subsequent Water Detection System Controlled by the MicrocontrollerNooria Sukmaningtyas
This article instructs the subsequent water detection system design and implementation of sanitary
ware. This system used C8051f040 Microcontroller as the main control module and communication
module which controlled the operation of the entire system, touch screen as a position machine,and
made use of metal probing technique and weighing sensor technology to realize data test, collection,
display, storage and export. At last, the experimental results showed that this system meets the expected
requirement and can measure the subsequent water more accurately.
Biodegradation of CAHs in River SedimentsGeoHydChem
SEDBARCAH - SEDiment BioBARriers for Chlorinated Aliphatic Hydrocarbons in ground water reaching surface water, European Project No 511254 (2005-2007).
Task D13 - Numerical Simulation of Batch and Column Tests from Zenne and Bela Sites (CAH-Biodegradation Model).
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
Successful detection of 40 COSMIC hotspot mutations at allelic frequency belo...Thermo Fisher Scientific
Research has shown that circulating tumor DNA (ctDNA) is
informative of tumor load and tumor evolution in both solid and
hematological cancer. The ability to detect mutations in ctDNA
holds the promise for an accurate and non-invasive approach to
assess minimum residual disease as well as treatment
response in the future. However, as ctDNA often makes up only
a small fraction of cell-free DNA recovered from the plasma,
traditional methods of targeted sequencing research often face
a poor signal-to-noise ratio that cannot be overcome with deep
coverage.
Here we present a novel research method that is capable of
detecting ultra-rare mutations at allelic frequency below 0.5%.
This approach leverages target multiplexing capabilities of the
Ion AmpliSeq™ technology with some important modifications
to the sample preparation procedures. The new protocol
requires as little as 20 ng of input DNA and offers a sample-toanswer
turn-around time in under 24 hours. To support the
analysis of this new approach, we have further developed a
novel Bayesian statistics that models the propagation of
potential artifacts introduced during amplification and sampling
effects during sequencing to differentiate false positives
(variants observed in sequencing data that were not present in
input DNA) from true mutations that were present at very low
levels in the original research sample.
We successfully applied this new method to detect spike-in
mutant DNA in both cell line (Coriell GM24385) and cfDNA
samples. Specifically, we demonstrated the detection of 140
COSMIC genomic aberrations found in 23 frequently mutated
genes. In preliminary study, the method achieved greater 90%
sensitivity and specificity.
Gas Chromatography Thermo Fisher ScientificYuniarHasani
What is Chromatography?
Separation process that is achieved by distributing the analytes to be separated
between a mobile phase and a stationary phase.
Sample transported by mobile phase
Some components in sample interact more strongly with stationary phase
and are more strongly retained
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
Analysis of Cations in Hydraulic Fracturing Flowback Water from the Marcellus Shale Using Ion Chromatography
This presentation describes the determination of cations in hydraulic fracturing flowback water using ion chromatography. In this work, sodium was most abundant, followed by calcium, strontium, magnesium, potassium, barium, ammonium, and then lithium, respectively. The quantity of scale-forming ions, such as calcium, strontium, and barium, is particularly informative because it can be used to determine the amount of anti-scaling agent in fracturing fluid mix that will maximize hydrocarbon recovery.
Design of a Subsequent Water Detection System Controlled by the MicrocontrollerNooria Sukmaningtyas
This article instructs the subsequent water detection system design and implementation of sanitary
ware. This system used C8051f040 Microcontroller as the main control module and communication
module which controlled the operation of the entire system, touch screen as a position machine,and
made use of metal probing technique and weighing sensor technology to realize data test, collection,
display, storage and export. At last, the experimental results showed that this system meets the expected
requirement and can measure the subsequent water more accurately.
Biodegradation of CAHs in River SedimentsGeoHydChem
SEDBARCAH - SEDiment BioBARriers for Chlorinated Aliphatic Hydrocarbons in ground water reaching surface water, European Project No 511254 (2005-2007).
Task D13 - Numerical Simulation of Batch and Column Tests from Zenne and Bela Sites (CAH-Biodegradation Model).
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
Successful detection of 40 COSMIC hotspot mutations at allelic frequency belo...Thermo Fisher Scientific
Research has shown that circulating tumor DNA (ctDNA) is
informative of tumor load and tumor evolution in both solid and
hematological cancer. The ability to detect mutations in ctDNA
holds the promise for an accurate and non-invasive approach to
assess minimum residual disease as well as treatment
response in the future. However, as ctDNA often makes up only
a small fraction of cell-free DNA recovered from the plasma,
traditional methods of targeted sequencing research often face
a poor signal-to-noise ratio that cannot be overcome with deep
coverage.
Here we present a novel research method that is capable of
detecting ultra-rare mutations at allelic frequency below 0.5%.
This approach leverages target multiplexing capabilities of the
Ion AmpliSeq™ technology with some important modifications
to the sample preparation procedures. The new protocol
requires as little as 20 ng of input DNA and offers a sample-toanswer
turn-around time in under 24 hours. To support the
analysis of this new approach, we have further developed a
novel Bayesian statistics that models the propagation of
potential artifacts introduced during amplification and sampling
effects during sequencing to differentiate false positives
(variants observed in sequencing data that were not present in
input DNA) from true mutations that were present at very low
levels in the original research sample.
We successfully applied this new method to detect spike-in
mutant DNA in both cell line (Coriell GM24385) and cfDNA
samples. Specifically, we demonstrated the detection of 140
COSMIC genomic aberrations found in 23 frequently mutated
genes. In preliminary study, the method achieved greater 90%
sensitivity and specificity.
Gas Chromatography Thermo Fisher ScientificYuniarHasani
What is Chromatography?
Separation process that is achieved by distributing the analytes to be separated
between a mobile phase and a stationary phase.
Sample transported by mobile phase
Some components in sample interact more strongly with stationary phase
and are more strongly retained
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
www.envimart.vn - ĐT: 028 77727979 - sales@envimart.vn - Nền tảng cung cấp thiết bị, vật tư ngành nước và môi trường. Chuyên cung cấp vật tư cho dự án xử lý nước sạch, nước thải và môi trường. Envimart luôn đồng hành, tin cậy với đối tác nhà thầu, nhà tích hợp và người sử dụng.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
1. ic_sampl_prep_26062003_e.ppt 1
IC IC
IC IC
IC
Metrohm inline sample preparation
Techniques for analytical success
Metrohm Ion Chromatography
Bart Cleeren, Metrohm België
2. ic_sampl_prep_26062003_e.ppt 2
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
3. ic_sampl_prep_26062003_e.ppt 3
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
4. ic_sampl_prep_26062003_e.ppt 4
IC IC
IC IC
IC
Metrohm Ion Chromatography
Ultrafiltration
Inline sample preparation
Ultrafiltration cell
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
S Sample
P2 Peristaltic pump
A position fill
C
P1
E D
2
5. ic_sampl_prep_26062003_e.ppt 5
IC IC
IC IC
IC
Metrohm Ion Chromatography
Ultrafiltration Cell
Inline sample preparation
No particles on the acceptor side
Big particles are flushed away
and do not clog the membrane
High flow on the sample side
Low flow on the acceptor side
6. ic_sampl_prep_26062003_e.ppt 6
IC IC
IC IC
IC
Metrohm Ion Chromatography
Ultrafiltration
Inline sample preparation
# 1 Sample filtration
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
S Sample
P2 Peristaltic pump
A position fill
C
P1
E D
2
7. ic_sampl_prep_26062003_e.ppt 7
IC IC
IC IC
IC
Metrohm Ion Chromatography
Ultrafiltration
Inline sample preparation
# 2 Sample injection
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
S Sample
P2 Peristaltic pump
A position inject
C
P1
E D
2
8. ic_sampl_prep_26062003_e.ppt 8
IC IC
IC IC
IC
Metrohm Ion Chromatography
Ultrafiltration: vegetable juice
Inline sample preparation
A Supp 4 – 250
1 Organics n.q.
2 Organics n.q.
3 Cl– 4141
4 NO3
– 102
5 HPO4
2– 577
6 SO4
2– 842
7 Oxalate n.q.
ppt
NaHCO3/Na2CO3; 1.7/1.8 mmol/L;
dilution 1:100; 0.15 µm rec. cellul.
9. ic_sampl_prep_26062003_e.ppt 9
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
10. ic_sampl_prep_26062003_e.ppt 10
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Dialysis cell, acceptor solution
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
P3 Peristaltic pump (2 Ch.)
Dialysis cell
A Acceptor solution
Dialysis
C
P1
E D
2
A E
P1
P2
P3
A D
C
X
X
11. ic_sampl_prep_26062003_e.ppt 11
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 2 Stopped flow dialysis
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
Injection Valve «B»
Dialysis cell
A Acceptor solution
Dialysis
C
P1
E D
2
A
P3
P2
A
P1
C
D
E
12. ic_sampl_prep_26062003_e.ppt 12
IC IC
IC IC
IC
Metrohm Ion Chromatography
Dialysis cell
Inline sample preparation
Continuous flow dialysis
Exclusion diameter 0.2 µm
Ions can pass
Oil droplets, fat micelles, particles
are excluded
Strong concentration gradient
No equilibrium concentration
Cellulose acetate membrane
13. ic_sampl_prep_26062003_e.ppt 13
IC IC
IC IC
IC
Metrohm Ion Chromatography
Dialysis cell
Inline sample preparation
Stopped flow dialysis
Dialysis time 2 ... 8 min
Sample volume 10 mL
No loss of concentration on the
sample side
Equilibrium concentration
Dialysis rate > 99 %
No filtration, no pre concentration
Unique technique, that had
significantly improves the field of
application for IC
14. ic_sampl_prep_26062003_e.ppt 14
IC IC
IC IC
IC
Metrohm Ion Chromatography
Dialysis: milk
Inline sample preparation
Metrosep Dual 1 – 70
1 Chloride 938
2 Nitrate n.q.
3 Phosphate n.q
4 Sufate 103
ppm
NaHCO3/Na2CO3; 2.4/2.0 mmol/L;
dilution 1:5; 0.2 µm cellul. acetat
15. ic_sampl_prep_26062003_e.ppt 15
IC IC
IC IC
IC
Metrohm Ion Chromatography
Dialysis: cutting oil emulsion
Inline sample preparation
Metrosep Dual 2
1 Chloride 74.7
2 Nitrite 20.5.
3 Nitrate 23.9
4 n. det. -
5 Phosphate 25.2
6 Sulfate 192.7
7 n. det. -
NaHCO3/Na2CO3; 2.0/1.3 mmol/L;
dilution 1:5; 0.2 µm cellul. acetat
16. ic_sampl_prep_26062003_e.ppt 16
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
17. ic_sampl_prep_26062003_e.ppt 17
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 1 Sample preconcentration
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
C
P1
D
P3 2
S
Inline pre concentration
E
C
P1
P 2
D
18. ic_sampl_prep_26062003_e.ppt 18
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 2 Sample analysis
E Mobile phase: eluent
P 1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P 2 Peristaltic pump
C
P1
E D
P3 2
S
Inline preconcentration
E
C
D
P1
P 2
19. ic_sampl_prep_26062003_e.ppt 19
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline preconcentration: ultra pure water
Inline sample preparation
Metrosep A Supp 5 – 150
1 Acetate 2.3
2 Formate 22.0
3 Chloride 13.0
4 Carbonate n.q.
5 Nitrate 4.5
6 Phosphate 16.0
7 Sulfate 8.5
8 Oxalate 12.0
ppt
NaHCO3/Na2CO3; 1.0/3.2 mmol/L;
20 mL preconc,
20. ic_sampl_prep_26062003_e.ppt 20
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline preconcentration: ultra pure water
Inline sample preparation
Metrosep Dual 2
1 Fluoride 45 – 150
2 Chloride 100 – 210
3 Bromide 0 – 100
4 Nitrate 10 – 112
5 Sulfate 40 – 145
ppt
NaHCO3/Na2CO3; 2.0/3.2 mmol/L;
10 mL preconc, spiked 100 ppt
21. ic_sampl_prep_26062003_e.ppt 21
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
22. ic_sampl_prep_26062003_e.ppt 22
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
Transfer solution «T»
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
Injection Valve «B»
P3 Peristaltic pump
T Transfer solution
A position fill, B position fill
C
P1
E D
2
T
Matrix elimination
23. ic_sampl_prep_26062003_e.ppt 23
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 1 Preconcentrator column inline
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
Injection Valve «B»
P3 Peristaltic pump
T Transfer solution
A position fill, B position fill
C
P1
E D
2
T
Matrix elimination
24. ic_sampl_prep_26062003_e.ppt 24
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 2 Sample transfer, matrix elim.
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
Injection Valve «B»
P3 Peristaltic pump
T Transfer solution
A position fill, B position inject
C
P1
E D
2
T
Matrix elimination
25. ic_sampl_prep_26062003_e.ppt 25
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 3 Sample inject
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2 Peristaltic pump
Injection Valve «B»
P3 Peristaltic pump
T Transfer solution
A position inject, B position inject
C
P1
E D
2
T
Matrix elimination
26. ic_sampl_prep_26062003_e.ppt 26
IC IC
IC IC
IC
Metrohm Ion Chromatography
Matrix elimination: organic solvents
Inline sample preparation
50 µL iPrOH
B with matrix elimination
A without matrix elimination
ppt
F–
Ac–
443 Cl–
30 NO3
– 28 SO4
2–
425 Cl–
24 NO3
– 17 SO4
2–
27. ic_sampl_prep_26062003_e.ppt 27
IC IC
IC IC
IC
Metrohm Ion Chromatography
Matrix elimination: 35 % H2O2
Inline sample preparation
left:
without m.e.
Loop 20 µL
right:
with m.e.
Loop 20 µL
transfer vol . 2 mL
28. ic_sampl_prep_26062003_e.ppt 28
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
29. ic_sampl_prep_26062003_e.ppt 29
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
833.0030 IC Sample Prep. Unit
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2, 3 Peristaltic pumps
Injection Valve «B»
T Transfer solution
833 IC Sample Prep
A position inject, B position fill
C
P1
E D
2
T
Matrix neutralization
30. ic_sampl_prep_26062003_e.ppt 30
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 1 Preconcentrator column inline
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2, 3 Peristaltic pumps
Injection Valve «B»
T Transfer solution
833 IC Sample Prep
A position fill, B position fill
C
P1
E D
2
T
Matrix neutralization
31. ic_sampl_prep_26062003_e.ppt 31
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 2 Sample transfer/neutralization
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2, 3 Peristaltic pumps
Injection Valve «B»
T Transfer solution
833 IC Sample Prep
A position fill, B position inject
C
P1
E D
2
T
Matrix neutralization
32. ic_sampl_prep_26062003_e.ppt 32
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
# 4 Sample inject
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2, 3 Peristaltic pumps
Injection Valve «B»
T Transfer solution
833 IC Sample Prep
A position inject, B position inject
C
P1
E D
2
T
Matrix neutralization
33. ic_sampl_prep_26062003_e.ppt 33
IC IC
IC IC
IC
0.74 F–
3.6 Cl–
0.97 NO3
–
0.98 HPO4
2–
1.1 SO4
2–
Metrohm Ion Chromatography
Matrix neutralization: caustic soda
Inline sample preparation
30 % NaOH, 20 µL loop
B with matrix elimination
A without matrix elimination
ppm
34. ic_sampl_prep_26062003_e.ppt 34
IC IC
IC IC
IC
Metrohm Ion Chromatography
Matrix neutralization: caustic soda
Inline sample preparation
Metrosep Dual 2
1 Chloride 25.08
2 Carbonate n.q.
3 Chlorate 4.76
4 Sulfate 10.43
ppm
NaHCO3/Na2CO3; 1.7/1.8 mmol/L;
50 % NaOH, 20 µL loop
35. ic_sampl_prep_26062003_e.ppt 35
IC IC
IC IC
IC
1
Metrohm Ion Chromatography
Matrix neutralization: ammonia
Inline sample preparation
Metrosep A Supp 4 – 250
1 Formate n.q.
2 Chloride 0.03
3 Nitrite n.q.
4 Bromide 0.95
5 Nitrat 0.04
6 Carbonate n.q.
7 Sulfate 0.13
ppm
NaHCO3/Na2CO3; 1.7/1.8 mmol/L;
26 % NH3, 20 µL loop
2
3
4
5 6
7
36. ic_sampl_prep_26062003_e.ppt 36
IC IC
IC IC
IC
P3
Metrohm Ion Chromatography
Inline sample preparation
OH cartridge
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P2, 3 Peristaltic pumps
Injection Valve «B»
T Transfer solution
Cartridge
A position fill, B position fill
C
P1
E D
2
T
Matrix neutralization
Ctr
37. ic_sampl_prep_26062003_e.ppt 37
IC IC
IC IC
IC
Metrohm Ion Chromatography
Matrix neutralization: HCl
Inline sample preparation
10 µL
HCl direct injection
ppm
Metrosep C2 – 250
38. ic_sampl_prep_26062003_e.ppt 38
IC IC
IC IC
IC
Metrohm Ion Chromatography
Matrix neutralization: HCl
Inline sample preparation
Metrosep C2 – 250
1 Nickel n.q.
2 Sodium 5.4
3 Ammonium 1.3
4 Potassium 0.8
5 Magnesium 1.5
6 Calcium 0.8
ppm
Tartaric-/dipicolinic acid; 4.0/0.75
mmol/L; 7 mol HCl, 10 µL loop
1
2
3
4 5 6
39. ic_sampl_prep_26062003_e.ppt 39
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Ultra filtration
Stop flow dialysis
Pre concentration technique
Matrix elimination
Matrix neutralization
In- line calibration
40. ic_sampl_prep_26062003_e.ppt 40
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
Standard S, flow path
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P1,2,3 Peristaltic pumps
Injection Valve «B»
S Standard
A position inject, B position fill
Inline calibration
C
P1
E D
P3 2
S
41. ic_sampl_prep_26062003_e.ppt 41
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 1 Measurement of standard
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P1,2,3 Peristaltic pumps
Injection Valve «B»
S Standard
A position inject, B position fill
C
P1
E D
P3 2
S
Inline calibration
42. ic_sampl_prep_26062003_e.ppt 42
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 2 Preconcentrator inline
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P1,2,3 Peristaltic pumps
Injection Valve «B»
S Standard
A position fill, B position fill
C
P1
E D
P3 2
S
Inline calibration
43. ic_sampl_prep_26062003_e.ppt 43
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 3 Transfer of standard
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P1,2,3 Peristaltic pumps
Injection Valve «B»
S Standard
A position fill, B position inject
C
P1
E D
P3 2
S
Inline calibration
44. ic_sampl_prep_26062003_e.ppt 44
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 4 Inject of standard
E Mobile phase: eluent
P1 High pressure pump
Injection valve «A»
C Analytical column
»MSM» Suppressor
D Conductivity detector
P1,2,3 Peristaltic pumps
Injection Valve «B»
S Standard
A position inject, B position inject
C
P1
E D
P3 2
S
Inline calibration
45. ic_sampl_prep_26062003_e.ppt 45
IC IC
IC IC
IC
Metrohm Ion Chromatography
Inline sample preparation
# 1: Transfer of 2 mL ultrapure
water to the preconcentrator
Blank value
# 2: Transfer of 2 mL ultrapure
water to the preconcentrator.
Valve B is thrown from fill to inject
and back once
Calibration level 1
# 3: Transfer of 2 mL ultrapure
water to the preconcentrator.
Valve B is thrown from fill to inject
and back twice
Calibration level 2
# 4: Transfer of 2 mL ultrapure
water to the preconcentrator.
Valve B is thrown from fill to inject
and back three times
Calibration level 3, etc
Inline calibration