This document discusses aseptic measures and techniques used to prevent infection. It defines asepsis as practices that decrease or eliminate infectious agents and transmission. The goal of aseptic technique is to protect patients from infection by maintaining absence of pathogens. Key aspects of asepsis include proper hand hygiene, use of personal protective equipment, cleaning and disinfection of surfaces and equipment, and separation of contaminated and clean areas/items. Various sterilization methods like steam, boiling water and radiation are used to destroy all microorganisms.