Two mycobacteriophages, Incognito and Serotinus, were isolated from tropical soil samples in Puerto Rico. Incognito was isolated from a soil sample taken 10 cm deep under a plantain tree in Hatillo, Puerto Rico on March 8, 2014. After purification, Incognito was determined to likely be a temperate phage. Serotinus was isolated from a soil sample taken 2.3 cm deep underneath a decaying fruit in Cayey, Puerto Rico on March 20, 2014. Serotinus was purified and determined to likely be a lytic phage. Both phages were purified to obtain medium titer phage lysates.