This data sheet contains absorbance readings from a protein purification experiment conducted by students Paola Caballero, Kenny Colón, Alejandra De Jesús, and Anthony Hernández from Group 1. Absorbance values were recorded at 280nm for samples including a blank, load, spent, and multiple washes and eluates. The data will be analyzed to assess the purification and concentration of the protein of interest across the purification steps.