.DEFINITION OF FISH PHARMACOLOGY:
“Fish pharmacology is essential for undertaking treatment of fishes using any therapeutic chemicals or drugs.”
“Pharmacology is the study of the interaction of chemicals with living system.”
“Pharmacology” is morden science which correlated other biological sciences, eg., Biochemistry , Physiology Microbiology , Medicine , and Genetics.
“Pharmacology is the branch of biology concerned with the study of drug action.”
“Pharmacology is the study of drugs including their origins, history, uses, and properties. It mainly focuses on the actions of drugs on the body.”
“Pharmacology is the study of drugs and theire actions on the body”.
“Pharmacology is the study of substance that interact with living systems through chemical process, especially by binding to regulatory molecules &activating or inhibiting normal body process.
Fig. 1
3. HISTORY OF FISH PHARMACOLGY:
Pharmacology emerged as its own discipline in the 19th Century, branching off from research done in fields of science such as organic chemistry and physiology. Oswald Schmiedeberg, who was born in what is now Latvia in 1838, is considered the father of pharmacology. His doctoral thesis was on the measurement of chloroform levels in blood, and he went on to become a professor of pharmacology at the University of Strasburg, where he ran an institute of pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mushroom Amanita muscaria that stimulates the parasympathetic nervous system and has been used to treat various diseases such as glaucoma.
In 1890, John Jacob Abel became the first pharmacology chair in the United States, at the University of Michigan. He later went to Johns Hopkins University in Baltimore. Abel was the first to isolate the hormone epinephrine from the adrenal gland, isolate histamine from the pituitary gland, and make pure crystalline insulin. Animals such as dogs, cats, pigeons, and frogs were used to test pharmacological substances. Humans were even used as test subjects. Sometimes they suffered through severe adverse effects from these substances, such as when the German pharmacist Friedrich Serturner and three of his friends had poisoning for several days from an alkaloid that Serturner had isolated from opium. This alkaloid was later named morphine, after the Ancient Greek god of sleep, Morpheus.
Oswald Schmiedeberg was a brilliant scientist. He studied the pharmacology of various compounds, including chloroform, and published an important text called the Outline of Pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mush to the field, Schmiedeberg is now known as 'the father of pharmacology'.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
.DEFINITION OF FISH PHARMACOLOGY:
“Fish pharmacology is essential for undertaking treatment of fishes using any therapeutic chemicals or drugs.”
“Pharmacology is the study of the interaction of chemicals with living system.”
“Pharmacology” is morden science which correlated other biological sciences, eg., Biochemistry , Physiology Microbiology , Medicine , and Genetics.
“Pharmacology is the branch of biology concerned with the study of drug action.”
“Pharmacology is the study of drugs including their origins, history, uses, and properties. It mainly focuses on the actions of drugs on the body.”
“Pharmacology is the study of drugs and theire actions on the body”.
“Pharmacology is the study of substance that interact with living systems through chemical process, especially by binding to regulatory molecules &activating or inhibiting normal body process.
Fig. 1
3. HISTORY OF FISH PHARMACOLGY:
Pharmacology emerged as its own discipline in the 19th Century, branching off from research done in fields of science such as organic chemistry and physiology. Oswald Schmiedeberg, who was born in what is now Latvia in 1838, is considered the father of pharmacology. His doctoral thesis was on the measurement of chloroform levels in blood, and he went on to become a professor of pharmacology at the University of Strasburg, where he ran an institute of pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mushroom Amanita muscaria that stimulates the parasympathetic nervous system and has been used to treat various diseases such as glaucoma.
In 1890, John Jacob Abel became the first pharmacology chair in the United States, at the University of Michigan. He later went to Johns Hopkins University in Baltimore. Abel was the first to isolate the hormone epinephrine from the adrenal gland, isolate histamine from the pituitary gland, and make pure crystalline insulin. Animals such as dogs, cats, pigeons, and frogs were used to test pharmacological substances. Humans were even used as test subjects. Sometimes they suffered through severe adverse effects from these substances, such as when the German pharmacist Friedrich Serturner and three of his friends had poisoning for several days from an alkaloid that Serturner had isolated from opium. This alkaloid was later named morphine, after the Ancient Greek god of sleep, Morpheus.
Oswald Schmiedeberg was a brilliant scientist. He studied the pharmacology of various compounds, including chloroform, and published an important text called the Outline of Pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mush to the field, Schmiedeberg is now known as 'the father of pharmacology'.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
A brief presentation on fish vaccination and its application particularly in Bangladesh. The overall process is described in a nutshell here. The types, procedure of formation, regulation, licensing and use are among them.
Finfish breeding and hatchery management pdfKartik Mondal
Rivers were the major source of freshwater fish seed in India during 1950’s and 1960’s.
Over the years, the riverine contribution has declined and at present forms only a supplementary source, constituting less than 5% of the country’s total fish seed production.
The Ganga, the Brahmaputra and the Indus river systems in the North and the Peninsular East coast and the West coast river systems in the South are the important natural sources of fish seed.
Antiseptics and disinfectants in aquaculture can be accepted alternative in minimizing use of antibiotics, if they meet maximum efficiency and minimum impact on fish health and the environment.
Defence mechanism in finfish and shellfish jassi 2Jaspreet Singh
Farming of fish and shellfish has gained significant grounds in several parts of the world .
Now a days disease is main problem in aquafarming.
In recent years ,lot of attention is being given to health management using various forms of immunoprophylactic techniques such as vaccination and immunostimulation .
To reduce immunoprophylactic application ,it is vital to have insight into specific and non specific defense mechanism of farmed animal .
Through disease process studies ,it is very well known that a pathogen can cause disease only if it can overcome the non specific and specific defense barriers of the host and successfully establish and proliferate
A brief info on the immunostimulants and probiotics in aquaculture. hope it will help whoever visits and go through the seminar.
Please comment if any mistakes found for my rectification as well as for others.
thank you
A brief presentation on fish vaccination and its application particularly in Bangladesh. The overall process is described in a nutshell here. The types, procedure of formation, regulation, licensing and use are among them.
Finfish breeding and hatchery management pdfKartik Mondal
Rivers were the major source of freshwater fish seed in India during 1950’s and 1960’s.
Over the years, the riverine contribution has declined and at present forms only a supplementary source, constituting less than 5% of the country’s total fish seed production.
The Ganga, the Brahmaputra and the Indus river systems in the North and the Peninsular East coast and the West coast river systems in the South are the important natural sources of fish seed.
Antiseptics and disinfectants in aquaculture can be accepted alternative in minimizing use of antibiotics, if they meet maximum efficiency and minimum impact on fish health and the environment.
Defence mechanism in finfish and shellfish jassi 2Jaspreet Singh
Farming of fish and shellfish has gained significant grounds in several parts of the world .
Now a days disease is main problem in aquafarming.
In recent years ,lot of attention is being given to health management using various forms of immunoprophylactic techniques such as vaccination and immunostimulation .
To reduce immunoprophylactic application ,it is vital to have insight into specific and non specific defense mechanism of farmed animal .
Through disease process studies ,it is very well known that a pathogen can cause disease only if it can overcome the non specific and specific defense barriers of the host and successfully establish and proliferate
A brief info on the immunostimulants and probiotics in aquaculture. hope it will help whoever visits and go through the seminar.
Please comment if any mistakes found for my rectification as well as for others.
thank you
A survey made at the end of an aqua industry forum meeting in Vietnam last year has shown that for 63 percent of the participants, the most limiting challenge for developing aquaculture was health and disease management. Indeed, in recent years, we have seen numerous diseases appearing and impacting aquaculture production, such as WSSV and EMS in shrimp, or Infectious Salmon Anemia (ISA) in salmonids. Working around the classic Host-Pathogen-Environment triad, new technologies and management techniques have been developed to better control diseases in aquatic animals: vaccination, which has led to the decrease of antibiotic use in salmonids; biosecurity procedures in hatcheries and in farms; biofloc technology. All of these technologies have proven successful. Their further development and expanded use will certainly improve the way aquatic animals are farmed.
Presentation during the Bureau of Agricultural Research (BAR) Seminar Series on June 22, 2017 at RDMIC Bldg., cor. Visayas Ave., Elliptical Rd., Diliman, Quezon City
We investigated the effects of fish protein hydrolysate (FPH) on zootechnical performance and immune response of the Asian Seabass Lates calcarifer Bloch. Experimental fish were fed with 3 diets: a local commercial diet (control), coated or not, with 2 and 3% FPH (w/w). Twelve thousand Asian Seabass juveniles (5.88±0.56 g) were divided into three groups and two replicates reared in nursery tanks (2000 L). The remaining fish were then used for grow-out experiment in floating net cages (1m x 1 m x 3 m). Zootechnical performances were assessed at both stages with following indicators: total weight gain (TWG), % relative weight gain (% RWG), % specific growth rate (% SGR), final weight (g) and final length (cm). At the end of each trial period, fish immune status was assessed through blood sampling and the measurement of Neutrophile (%), Monocyte (%), Lymphocyte (%), Macrophage (105 cell/mL), Leukocyte (103 cell/mL) and Phagocytes activity (%). At the end of the nursery trial, an immersion bacterial challenge with Vibrio parahaemolyticus (105 cells mL-1) was implemented. The results showed that dietary FPH supplementation significantly influenced the growth and immune status of Asian Seabass when compared to the control group. Fish fed FPH supplemented diet yielded higher growth rates and survival rates than non supplemented group. Fish phagocytic activity and resistance to a bacterial challenge were also improved by dietary FPH supplementation. These results may be related to the significant changes observed in fish leukocyte profiles, when fed FPH supplemented diets. Altogether, these results show the positive contribution of FPH to the sustainability of Asian seabass farming.
Effects of chemotherapeutics against experimentally injured stinging catfish ...AbdullaAlAsif1
p>Effects of different chemotherapeutics were examined against experimentally infected stinging catfish Heteropneustes fossilis . Fish were collected from a fish market in Mymensingh, acclimatized for 7 days in laboratory condition from January to February, 2016 in aquaria at Fish Clinic of Bangladesh Agricultural University, Mymensingh, experimentally injured by using forceps and knife and waited seven days for the establishment of infection. Before starting chemotherapeutic trial, it was confirmed that the fish were infected with microorganisms by clinical diagnosis in laboratory condition. They showed hemorrhages and ulcerative lesions over the infected area. A total of 80 such experimentally infected stinging catfish having average body weight of 18 g were used for the experiment. Two chemotherapeutics: antibiotic, Eryvet (erythromycin thiocyanate INN, sulphadiazine (NaUSP) & trimithoprim BP) and antifungal, methylene blue were used in separate and combined treatment with three different doses of antibiotic. The same dose of methylene blue, 0.2 mg/l, was used for separate and combined treatment by antibiotic. Doses of antibiotic (Eryvet) were 0.8 g/10 kg body weight of fish, 1g/10 kg body weight of fish and 1.2 g/10 kg body weight of fish as lower dose, recommended dose and higher dose respectively. For combined treatment the above different doses of antibiotic and the same dose of antifungal were used. Water was exchanged regularly. The chemotherapeutic trial was conducted for 7 days and observation was continued for another 8 days to observe the effect of treatment. Combined treatment with the recommended dose of the antibiotic and methylene blue showed the best result where 90% fish were recovered. By the treatment with the higher dose of the antibiotic 70% fish were recovered. Antifungal treatment showed that 20% fish were recovered. All the fish in negative control aquarium died.
The effect of stress caused by environmental pollution and farming conditions on the health and yield of fish in intensive aquacultures is becoming increasingly important.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...
Immunostimulants role in aquaculture
1. Dr. Mandeep Kaur
Role Of Immunostimulants in
Combating Disease Outbreak in
Aquaculture
PGGCG, Sec- 42, Chandigarh, India
Mandeep.kaur15@hotmail.com
2. Introduction
• Aquaculture: represents fast growing food producing
sectors.
• Intensive farming under high population density give a way
to infectious diseases which pose a constant and high cost
threat to aquaculture.
• The best way to overcome the disease problems in a system
is through effective management practices.
• As proper management is not always delivered, pathogens
become established in animals and produce disease.
3. Therefore, instead of antibiotics and
chemotherapeutic agents, increasing attention is being
paid to the use of immunostimulants for disease
control measures in aquaculture.
Use of expensive chemotherapeutants and antibiotics bring:
• Accumulation in the tissue as residues,
• Development of the drug resistance,
• Immuno-suppression and
• Reduced consumer preference for food fish treated with
antibiotics (Anderson, 1992).
4. Immunostimulants
Immunostimulants and immuno-modulators comprise a
group of biological and synthetic compounds that enhance
the cellular and humoral defense mechanism in animals
*Enhance defense mechanism
*Widely used for impaired immune function
*To stabilize the improved immune status.
The use of immunostimulants for the prevention of disease in fishes is
considered as an attractive and promising area in the field of aquaculture
(Secombes, 1994).
5. facilitate
the function of
phagocytic cells
increase their
bactericidal
activities
stimulate the
natural killer cells
stimulate
complement
system,
stimulate
lysozyme
activity
Stimulate
antibody
responses
Functions of
Immunostimulants
6. Innate Immunity
Initiation of specific
immune response
Success
No disease and
survival
Disease and death
Pathogen
Humoral response
(extracellular pathogens and toxin)
Cell mediated response(Intracellular
pathogen and viruses)
Acquired immunity, memory
and protection
7. FAO (UN) defined:
The development of affordable yet efficient vaccines, the use
of immunostimulants and non-specific immune enhancers,
and the use of probiotics and bio-augmentation methods for the
improvement of aquatic
environmental quality as major areas for further research in
disease control in Aquaculture (Mehana et al., 2014).
8. Types of Immunostimulants
Natural Synthetic
• Microbial derivative: β glucan,
LPS, bacterins
• Natural factors: Vit- C and Vit –
E
• Animal and Plant extracts
• Polysaccharides (Chitin ,
Chitosan, Lentinan)
• Hormones: (GH, Thyroid
hormone, prolactin), Cytokines
and others (ds RNA, recombinant
proteins)
•Macrogard
•Immersion grade
•Aquasalor
•Ergason (rich in
polysaccharides)
•Lomal
9. Most commonly used immunostimulants in
aquaculture
Most popular immunostimulants.
Derived from yeast cell wall and from certain higher plants.
It has excellent immuno-stimulatory properties and work well
when injected or fed to the fish.
Most common brands are MacroGard, Vetregard and EcoActiva
Glucan
ß-1,6, branched ß-1,3 Glucans were effective in stimulating the non-specific
immune response in carp (Yano et al., 1991)
10. Administration of glucan in carp enhanced survival, most likely
via stimulation of both non-specific and specific immune
reactions (superoxide anion, IL-1 secretion and antibody
formation), regardless of how it was administered (intraperitoneal
injection, bathing and oral administration) (Selvaraj et al., 2005). A
stimulation of complement and C reactive protein responses were
found in carp (Pionnier et al., 2013). Studies of glucan-activated
macrophages in trout revealed an increased ability to kill
salmonid pathogen Aeromonas salmonicida (Jorgensen et al.,
1993).
11. Levamisole
A synthetic Imidazothiazole, extensively used in both humans and
veterinary medicine as an anti-helminthic agent .
Levamisole is an effective treatment for Camallanus roundworm
infestations in freshwater tropical fish.
In fish, levamisole has been used in a few studies with the aim of
enhancing the non-specific immune response (Baba et al. 1993) or
as an adjuvant with a vaccine (Jeney and Anderson 1993).
12. Chitin
Linear Beta-1,4-linked polymer of N-acetyl-D-glucosamine,
A common constituent of insect and crustacean exoskeleton and fungal
cell wall.
Commercially manufactured from the shrimp and crab shells.
As chitin is a non-toxic biodegradable and biocompatible
substance, its derivatives are used in medical practice (Shibata et
al. 1997).
Increased protection against Aeromonas salmonicida has been
observed in brook trout when injected with chitin. Injection of
abalone extract and chitin increase phagocytic response and
natural killer cell activity in fish (Stickney, 2000).
13. Chitosan
Chitosan is a linear homopolymer of ß-(1,4)-2-amino-deoxy-D-
glucose and is prepared by the alkaline deacetylation of chitin
obtained from shrimp and crab shell.
Chitosan is used as an immunostimulant in aquaculture to
protect salmonids and carps against bacterial diseases (Anderson
and Siwicki 1994; Siwicki et al. 1994)
14. Natural plant products have been reported as antistress,
growth promotion, appetite stimulation, tonic
and immunostimulation, and to have aphrodisiac and
antimicrobial properties in finfish and shrimp larviculture
due
to the presence of active principle components such as
alkaloids, flavanoids, pigments, phenolics, terpenoids,
steroids, and essential oils (Citarasu et al. 2002; Sivaram
et al. 2004).
Use of herbal and medicinal plants as
immunostimulants in fish
15. Plant Disease/microbe
Ocimum sanctum Aeromonas hydrophila infection
Achyranthes aspera, Curcuma
longa and Allium sativum
Bacterial infections
seaweed extracts Vibrio parahaemolyticus
Withania somnifera Vibrio parahaemolyticus
Clinacanthus nutans protect shrimp from yellow head virus
(YHV) infection
Olea europaea leaf Salmonid rhabdovirus and viral
haemorrhagic septicaemia virus
(VHSV)
16. References
• Anderson DP. 1992. Immunostimulants, adjuvants and vaccine carrier in fish; application to Aquaculture. An Rev Fish
Dis 2: 281-307.
• Secombes CJ. 1994. Enhancement of fish phagocyte activity. Fish Shellfish Immunol 4: 421-436.
• Citarasu T. 2010. Herbal biomedicines: a new opportunity for aquaculture industry. Aqua Int 18: 403-414.
• Subasinghe R. 1997. Fish health and quarantine. In: Review of the state of the world aquaculture. FAO Fisheries
Circular No.
• 886. Food and Agriculture Organization of the United Nations, Rome, Italy: 45-49.
• Yano T, Matsuyama H, Mangindann REP. 1991. Polysaccharide- induced protection of carp, Cyprinus carpio against
bacterial infection. J Fish Dis 14: 557-582.
• Selvaraj V, Sampath K, Sekar V. Administration of yeast glucan enhances survival and some non-specific and specific
immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila. Fish Shellfish Immunol.
2005;9:293–306.
• Pionnier N, Falco A, Miest J, Frost P, Imazarow I, Shrive A, et al. Dietary β-glucan stimulates complement and C-
reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonidica infection.
Fish Shellfish Immunol. 2013;34:819–31
• Jorgensen JB, Sharp GJ, Secombes CJ, Robertsen B. Effects of a yeast-cell-wall glucan on the bactericidal activity of
rainbow trout macropages. Fish Shellfish Immunol. 1993;3:267–77.
• Baba T, Watase Y, Yoshinaga Y. 1993. Activation of mononuclear phagocytes function by levamisole immersion in carp.
Nippon Suisan Gakkaishi 59: 301-307
• Anderson DP, Jeney G. 1992. Immunostimulants added to injected Aeromonas salmonicida bacterin enhance the
defense
mechanisms and protection in rainbow trout (Oncorhynchus mykiss). Vet Immunol Immunopathol 34(3-4): 379-389
• Shibata Y, Metzger WJ, Myrvik Q. 1997b. Chitin particle-induced cell mediated immunity is inhibited by soluble
mannan. J
Immunol 159: 2462-2467.
• Stickney RR. 2000. Encyclopedia of aquaculture. Wiley-Interscience Press. pp. 676-679.
• Anderson DP, Swicki AK. 1994. Duration of protection against Aeromonas salmonicida in brook trout
immunostimulated with
glucan or chitosan by injection or immersion. Progres Fish Cul 56: 258-261.