This document discusses a study that assessed the effects of tannery effluent on seed germination and growth performance of two varieties of maize (BHQPY545 and Malkesa-2). The tannery effluent was found to be dark bluish green in color with a high biological oxygen demand and concentrations of total suspended solids and total dissolved solids above recommended standards. Laboratory experiments found that tannery effluent reduced seed germination percentage and seedling growth of both maize varieties in a concentration-dependent manner. Greenhouse experiments also observed reductions in vegetative growth and yield parameters with increasing effluent concentrations. The study concluded that the tannery effluent was not suitable for
Assessment of Canal Sediments for Agricultural Uses - JBESInnspub Net
The study was conducted to analyze the extent of heavy metals contamination and bacteriological content in the sediments of Lahore Branch Canal. The main purpose of digging this canal was that the areas of Lahore and Kasur could be irrigated for the means of agriculture. Due to improper drainage systems of industrial sectors and residential areas the level of pollution is increasing in the canal. Sediment samples were collected from three sites located at the canal intersections namely Thokar Niaz Baig, Punjab University New Campus and Dharampura during the canal closure period in 2014 and 2015. The samples were analyzed for heavy metals (Cadmium, Chromium, Copper, Cobalt, Zinc and Iron) by using Atomic Absorption Spectroscopy and the bacteriological content (Total coliform) was analyzed by pour plate method. The metals Zinc, Chromium, Copper and Cobalt were detected in minute concentration. Only the concentration of iron was higher than the recommended limit. According to the Codex Alimentarius standards, all the sediments samples were in safe limits. The Total coliform concentrations for the areas Thokar Niaz Baig, Punjab University and Dharampura were 2500, 5000 and 6000 colonies in year 2014 respectively. In 2015 the colonies of total coliform were found to be 1100, 4130 and 6400 in the same areas respectively. When these sediments are used for agricultural purposes the deposited metals present in them pose harm to the crops. When crops are eaten by human, the metals ultimately get transferred to human. Get the full articles at: http://www.innspub.net/jbes/assessment-of-canal-sediments-for-agricultural-uses/
Water quality analysis of bhishma lake at gadag cityeSAT Journals
Abstract The water bodies are facing a severe threat of pollution all over the world. Eutrophication in lakes is widespread world and the severity is increasing especially in the developing countries like India. The main objective is to study t haell eoffveecrt st hoef pwoelrleu ticoonll eocf tweda tefrro qmu aJlaitnyu ianr yB htios hAmpar illa kaen dd ubei -tmo ornatphildy utrebsatsn iwzaetrieo nc aanrrdi etdo ioduetn. tiTfyh eth pe hsyosuicrcoe-cs hoefm pioclalul tciohna rianc ltaekries.t iTchs eo sfa mwaptleers sdaismsopllvees dl iokxey gpeHn,, tBuOrbDid iwtye,r ea lakanlailnyiztye,d tiont aol rdhearr dtnoe sdse,t etromtailn ed itshseo llveevde ls oolfi dcso,n ntaitmraintea,n tpsh porsepsheantte ,i nc hlalokrei dwe,a tpeor taasnsdiu imts, psoosdsiiubmle, smoidtiiguamti,o nni tmraetaes, uprheso.s pWhaatteer, pqoutaalsitsiieusm s,u Dchi ssaos lvpeHd, OTxoytagle nD i(sDsoOlv) eadn sdo lBidios c(hTeDmSic),a lt uOrbxiydgietyn, Dalekmalainnidty ,( BtoOtDal) ,h aCrhdenmesicsa, lc hOloxyrigdeen, sDheomwaendd t h(CatO tDhe) wtoetrael dheatredrnmeisnse, dt ufrobri dwitayt,e rto staaml dpilsesso clvoellde cstoeldid fsr oamnd f iavelk astlaintiiotyn sv ainlu leask ee.x cTeheed iannga ltyhsei sd oesf irlaakbele w laimteirt paanrda mdueete rtos pCrheesmeniccael oOf xoyrggeann icD aenmda nindo r(gCaOnDic) phoallsu tiannctrse aDsiesdso lwvehdic hO xsyhgoewns (DthOe ) phoaosr reqduuaclietdy , oBfi owchaetemr icaanl dO ixsy gneont Dsueimtaabnlde (fBoOr Dh)u manadn consumption these values have been graphically plotted. Keywords: Lake Water Quality analysis, BOD, COD.
Productivity and quality of sugar beet as affecting by sowing and irrigation ...Innspub Net
Two field experiments were carried out at Kalabsho Experimental Farm, Dakahlia Governorate, Sugar Crops Research Institute, Agricultural Research Center, Egypt, during 2014/2015 and 2015/2016 seasons to study the effect of sowing methods (mechanical and manual methods), irrigation methods (surface and drip irrigation system) and hill spacings (10, 15 and 20cm between hills) on yield and its components, as well as quality of sugar beet cv. Hossam as a multigerm variety under sandy soil conditions. The obtained results could be summarized as follows; the optimum sowing method that produced the highest values of yield and its components as well as root quality parameters was mechanical sowing method (planter machine) in both seasons. Irrigation sugar beet plants by using drip irrigation system yielded the highest values of yield and its components as well as root quality parameters and followed by using surface flooding irrigation system in both seasons. Planting sugar beet seeds on one side of the ridge, 60cm width, and 20cm between hills, resulting plant population density 35000 plants/fed produced, the highest values of yield and its components and root quality parameters and followed by planting on 15cm between hills, resulting plant population density 46666 plants/fed and finally planting on 10cm between hills, resulting plant population density 70000 plants/fed in the two seasons. From the obtained data in this study, it can be concluded that sowing sugar beet using mechanical sowing method (planter machine), irrigation by using drip irrigation system and planting on one side of the ridge, 60cm width, and 20cm between hills, resulting plant population density 35000 plants/fed in order to maximizing its productivity and quality under the environmental conditions of sandy soil in Kalabsho region, Dakahlia Governorate, Egypt.
Water consumption
Water is one of the most important substances on earth. All plants and animals must have water to survive. If there was no water there would be no life on earth. There are two ways in which we can classify our water use. One type is in-stream use; this includes hydroelectric power, boating and swimming, for example. While in-stream activities do not use up the water, they can degrade the water quality through pollution. The other type of water use is the withdrawal of water, and this classification includes household use, industry use, irrigation, livestock watering and thermal and nuclear power. Most withdrawals are consumptions, meaning that the activity uses the water and does not return it to the source.
The amount of water that is taken (or withdrawn) from the source is called the water intake, and the amount that is returned is called the water discharge. The difference between the water intake and the water discharge is the amount consumed.
Water intake – Water discharge = Consumption
The total amount of water that is used is called the gross water use. The difference between the gross water use and the water intake is equal to the amount of water that is recirculated. The recirculated amount is expressed as a recycling rate and is a good indicator of water efficiency.
Gross water use – Water intake = Amount recirculated (or recycling rate)
Sources of water:
Ground Water:
Ground water refers to any source of water that lies beneath the soil layer. Ground water can exist in the soil itself or between rocks and other materials. Most communities obtain their water from underground aquifers, or rock formations capable of holding large amounts of freshwater. Only 3 percent of the water on earth is considered freshwater, with a mere 30 percent of that small amount being found as groundwater. Pollution, seawater contamination and overuse threaten this valuable resource.
Surface Water:
Sources of surface water can include any above-ground collection of water such as rivers, lakes, ponds and oceans. Some sources of surface water are also fed by underground aquifers. Surface water accounts for 80 percent of the water humans use.
Ocean Water:
Although ocean water makes up nearly 97 percent of all water on earth, it is not a viable source of potable water unless salt and other impurities are removed. Desalination, the process by which salt is removed from water, is a rapidly growing practice. While salt and other microscopic particles can be removed from water in a variety of ways, the most promising method is through reverse osmosis. This process forces saltwater through filters with microscopic pores that remove salt and other microbes. Reverse osmosis requires large amounts of energy, making it a very expensive process.
Assessment of Canal Sediments for Agricultural Uses - JBESInnspub Net
The study was conducted to analyze the extent of heavy metals contamination and bacteriological content in the sediments of Lahore Branch Canal. The main purpose of digging this canal was that the areas of Lahore and Kasur could be irrigated for the means of agriculture. Due to improper drainage systems of industrial sectors and residential areas the level of pollution is increasing in the canal. Sediment samples were collected from three sites located at the canal intersections namely Thokar Niaz Baig, Punjab University New Campus and Dharampura during the canal closure period in 2014 and 2015. The samples were analyzed for heavy metals (Cadmium, Chromium, Copper, Cobalt, Zinc and Iron) by using Atomic Absorption Spectroscopy and the bacteriological content (Total coliform) was analyzed by pour plate method. The metals Zinc, Chromium, Copper and Cobalt were detected in minute concentration. Only the concentration of iron was higher than the recommended limit. According to the Codex Alimentarius standards, all the sediments samples were in safe limits. The Total coliform concentrations for the areas Thokar Niaz Baig, Punjab University and Dharampura were 2500, 5000 and 6000 colonies in year 2014 respectively. In 2015 the colonies of total coliform were found to be 1100, 4130 and 6400 in the same areas respectively. When these sediments are used for agricultural purposes the deposited metals present in them pose harm to the crops. When crops are eaten by human, the metals ultimately get transferred to human. Get the full articles at: http://www.innspub.net/jbes/assessment-of-canal-sediments-for-agricultural-uses/
Water quality analysis of bhishma lake at gadag cityeSAT Journals
Abstract The water bodies are facing a severe threat of pollution all over the world. Eutrophication in lakes is widespread world and the severity is increasing especially in the developing countries like India. The main objective is to study t haell eoffveecrt st hoef pwoelrleu ticoonll eocf tweda tefrro qmu aJlaitnyu ianr yB htios hAmpar illa kaen dd ubei -tmo ornatphildy utrebsatsn iwzaetrieo nc aanrrdi etdo ioduetn. tiTfyh eth pe hsyosuicrcoe-cs hoefm pioclalul tciohna rianc ltaekries.t iTchs eo sfa mwaptleers sdaismsopllvees dl iokxey gpeHn,, tBuOrbDid iwtye,r ea lakanlailnyiztye,d tiont aol rdhearr dtnoe sdse,t etromtailn ed itshseo llveevde ls oolfi dcso,n ntaitmraintea,n tpsh porsepsheantte ,i nc hlalokrei dwe,a tpeor taasnsdiu imts, psoosdsiiubmle, smoidtiiguamti,o nni tmraetaes, uprheso.s pWhaatteer, pqoutaalsitsiieusm s,u Dchi ssaos lvpeHd, OTxoytagle nD i(sDsoOlv) eadn sdo lBidios c(hTeDmSic),a lt uOrbxiydgietyn, Dalekmalainnidty ,( BtoOtDal) ,h aCrhdenmesicsa, lc hOloxyrigdeen, sDheomwaendd t h(CatO tDhe) wtoetrael dheatredrnmeisnse, dt ufrobri dwitayt,e rto staaml dpilsesso clvoellde cstoeldid fsr oamnd f iavelk astlaintiiotyn sv ainlu leask ee.x cTeheed iannga ltyhsei sd oesf irlaakbele w laimteirt paanrda mdueete rtos pCrheesmeniccael oOf xoyrggeann icD aenmda nindo r(gCaOnDic) phoallsu tiannctrse aDsiesdso lwvehdic hO xsyhgoewns (DthOe ) phoaosr reqduuaclietdy , oBfi owchaetemr icaanl dO ixsy gneont Dsueimtaabnlde (fBoOr Dh)u manadn consumption these values have been graphically plotted. Keywords: Lake Water Quality analysis, BOD, COD.
Productivity and quality of sugar beet as affecting by sowing and irrigation ...Innspub Net
Two field experiments were carried out at Kalabsho Experimental Farm, Dakahlia Governorate, Sugar Crops Research Institute, Agricultural Research Center, Egypt, during 2014/2015 and 2015/2016 seasons to study the effect of sowing methods (mechanical and manual methods), irrigation methods (surface and drip irrigation system) and hill spacings (10, 15 and 20cm between hills) on yield and its components, as well as quality of sugar beet cv. Hossam as a multigerm variety under sandy soil conditions. The obtained results could be summarized as follows; the optimum sowing method that produced the highest values of yield and its components as well as root quality parameters was mechanical sowing method (planter machine) in both seasons. Irrigation sugar beet plants by using drip irrigation system yielded the highest values of yield and its components as well as root quality parameters and followed by using surface flooding irrigation system in both seasons. Planting sugar beet seeds on one side of the ridge, 60cm width, and 20cm between hills, resulting plant population density 35000 plants/fed produced, the highest values of yield and its components and root quality parameters and followed by planting on 15cm between hills, resulting plant population density 46666 plants/fed and finally planting on 10cm between hills, resulting plant population density 70000 plants/fed in the two seasons. From the obtained data in this study, it can be concluded that sowing sugar beet using mechanical sowing method (planter machine), irrigation by using drip irrigation system and planting on one side of the ridge, 60cm width, and 20cm between hills, resulting plant population density 35000 plants/fed in order to maximizing its productivity and quality under the environmental conditions of sandy soil in Kalabsho region, Dakahlia Governorate, Egypt.
Water consumption
Water is one of the most important substances on earth. All plants and animals must have water to survive. If there was no water there would be no life on earth. There are two ways in which we can classify our water use. One type is in-stream use; this includes hydroelectric power, boating and swimming, for example. While in-stream activities do not use up the water, they can degrade the water quality through pollution. The other type of water use is the withdrawal of water, and this classification includes household use, industry use, irrigation, livestock watering and thermal and nuclear power. Most withdrawals are consumptions, meaning that the activity uses the water and does not return it to the source.
The amount of water that is taken (or withdrawn) from the source is called the water intake, and the amount that is returned is called the water discharge. The difference between the water intake and the water discharge is the amount consumed.
Water intake – Water discharge = Consumption
The total amount of water that is used is called the gross water use. The difference between the gross water use and the water intake is equal to the amount of water that is recirculated. The recirculated amount is expressed as a recycling rate and is a good indicator of water efficiency.
Gross water use – Water intake = Amount recirculated (or recycling rate)
Sources of water:
Ground Water:
Ground water refers to any source of water that lies beneath the soil layer. Ground water can exist in the soil itself or between rocks and other materials. Most communities obtain their water from underground aquifers, or rock formations capable of holding large amounts of freshwater. Only 3 percent of the water on earth is considered freshwater, with a mere 30 percent of that small amount being found as groundwater. Pollution, seawater contamination and overuse threaten this valuable resource.
Surface Water:
Sources of surface water can include any above-ground collection of water such as rivers, lakes, ponds and oceans. Some sources of surface water are also fed by underground aquifers. Surface water accounts for 80 percent of the water humans use.
Ocean Water:
Although ocean water makes up nearly 97 percent of all water on earth, it is not a viable source of potable water unless salt and other impurities are removed. Desalination, the process by which salt is removed from water, is a rapidly growing practice. While salt and other microscopic particles can be removed from water in a variety of ways, the most promising method is through reverse osmosis. This process forces saltwater through filters with microscopic pores that remove salt and other microbes. Reverse osmosis requires large amounts of energy, making it a very expensive process.
Comparative Study of Test Driven Development TDD , Behavior Driven Developmen...ijtsrd
TDD, BDD and ATDD were introduced by XP Extreme Programming is an agile software development framework. They are unit testing approaches. TDD, BDD and ATDD are a software development technique which uses unit tests to incrementally deliver small pieces of functionality. TDD is a developer focused process. In Test Driven Development TDD , first come tests and then the code. The minimal piece of code is written in order to pass the designed test. In other words, it is the process of testing the code before its accrual writing. If the code passes the test, then developers can proceed to its refactoring. Behavior Driven Development BDD is a customer focused process. It is based on the full and clear understanding of the system or module behavior but in the terms of business client. The tests for TDD are created by developers for developers. The test for BDD can be written by testers or technical managers. Acceptance Test Driven Development ATDD is towards the developer focused side of things. ATDD is a technique where the entire team collaborates to define the acceptance criteria of a story before the implementation actually begins. These acceptance tests are supported necessary information. Using the Given When Then format, ATDD approach can implement. Myint Myint Moe ""Comparative Study of Test-Driven Development (TDD), Behavior-Driven Development (BDD) and Acceptance Test–Driven Development (ATDD)"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23698.pdf
Paper URL: https://www.ijtsrd.com/computer-science/other/23698/comparative-study-of-test-driven-development-tdd-behavior-driven-development-bdd-and--acceptance-test%C3%A2%E2%82%AC%E2%80%9Cdriven-development-atdd/myint-myint-moe
Investigation of Toxic Substance in Basra WatersIJCMESJOURNAL
There was a need to investigate the presence of toxic elements and compounds in drinking and desalination water for the population of the Basra city, especially after increasing cases of poisoning and diarrhea recently to more than 70,000 cases in the province of Basra according to the Human Rights Commission in the province . Six Samples (A, B, C, D, E, F) of drinking water and human uses of (RO) waters packaged in bottles produced by a private company (sample A), (RO) waters for direct sale (sample B) , waters desalination for the areas of Qibla, Jubaila and Brathaia (samples C,D,E) in addition to the waters of Shatt Al-Arab (sample F) for the purpose of conducting analysis and knowing the percentages of toxic elements and compounds in the compositions of these samples. The results of the analysis of the samples under the study confirmed that the ratio of total soluble solids (TDS) of the samples ( D, E , F ) to the other samples (A, B, C) ). The results of the analysis also confirmed the high percentage of the lead element (Pb) of the two samples (E, F) from the other samples. The results showed an increase in sulphate (SO4) for sample (E) than the other samples. The results showed an increase in the ratio of phosphate (PO4) of sample (F) to the rest of the samples in comparison with the standards of the World Health Organization (WHO). These results indicate that pollution in the Shatt Al-Arab waters increased with toxic elements and compounds. These results correspond to the statistics of the Directorate of Basra Heaith, where it showed the increase in the proportion of casualties among the population of the province of Basra to areas adjacent to the Shatt Al-Arab river, such as the city center , Abu- Khasseb and Al-Tanuma from the rest of the areas causing water pollution and the spread of E. coli causing diarrhea and intestinal colic with 50% of cases which was admitted to Basra hospitals.
Spearmint (Mentha spicata L.) Response to Deficit Irrigationpaperpublications3
Abstract: An experiment was conducted with the objective to identify the level of deficit irrigation which allows achieving optimal yield and investigate the effect of deficit irrigation practice on spearmint (Mentha Spicata L.) yield and yield components. The experiment was conducted at Koka research station of Wondo Genet Agricultural Research Center, Ethiopia latitude 8O26’, longitude 39O2’ and altitude 1602masl for two years (2011/2012 and 2012/2013 dry season). Nine treatments three level of irrigation water amount percentage based on evapo-transpiration of the crop (ETc) (100%ETc, 75%ETc and 50%ETc) and three types of furrow irrigation water application method (alternate furrow, fixed furrow and conventional furrow) were used in randomized completely block design (RCBD) with three replications. The pooled mean of two year data showed different level of deficit irrigation had a significant effect (p<0.05)><0.01) variation among treatments due to deficit irrigation on dry biomass yield per hectare, dry leaf yield per hectare, essential oil yield per hectare and water use efficiency. However, different deficit irrigation level had no significant influence on wet harvesting index. The highest fresh biomass, dry biomass, fresh leaf, dry leaf and essential oil yield per harvesting cycle of 12093kg/ha, 3746kg/ha, 8133kg/ha, 2441.1kg/ha and 37.0kg/ha respectively was obtained due to 100%ETc with conventional furrow application method. Moreover, the highest water use efficiency of 16.3x10-3kg/m3 was achieved due to deficit irrigation to 50%ETc with alternate furrow irrigation water application method. The study showed that the best treatment is deficit irrigation to 50%ETc with conventional furrow application method which had no significant variation with treatments that showed higher yields of spearmint including water use efficiency.
Keywords: Alternate furrow, conventional furrow, deficit irrigation, fixed furrow, spearmint.
Water Pollution is an undesirable change in physical, chemical or biological characteristics of water. Human population explosion, rapid industrialization, deforestation, unplanned urbanization, scientific and technological advancement etc. are mainly responsible for the pollution crisis on the earth.
Wastewater from manufacturing or chemical processes in industries contributes to water pollution. Industrial wastewater usually contains specific and readily identifiable chemical compounds.
During the last fifty years, the number of industries in India has grown rapidly. But water pollution is concentrated within a few sub sectors, mainly in the form of toxic wastes and organic pollutants. Out of this a large portion can be traced to the processing of industrial chemicals and to the food products industry.
The effects of water pollution are not only devastating to people but also to animals, fish, and birds. Polluted water is unsuitable for drinking, recreation, agriculture, and industry. It diminishes the aesthetic quality of lakes and rivers. More seriously, contaminated water destroys aquatic life and reduces its reproductive ability. Eventually, it is a hazard to human health. Nobody can escape the effects of water pollution.
Most major industries have treatment facilities for industrial effluents. But this is not the case with small-scale industries, which cannot afford enormous investments in pollution control equipment, as their profit margin is very slender. So the present study may be helpful for finding out methods for the treatment of effluents in batch scale that are cost effective so that small-scale industries can treat their wastes before releasing them into the environment.
The Effects of Using Magnetic Treatment Brackish Water in Irrigation on the Y...Agriculture Journal IJOEAR
Abstract— Over abstraction of groundwater and limitation of natural recharge in the Lower Jordan Valley are causing an increase in groundwater salinity. The high demand for medicine herbs at international market attracted Palestinian farmers to grow medicine herbs including Oregano. Pilot project was carried out under greenhouse condition where magnetize technology introduced for the first time in irrigation of Oregano to overcome salinity problem. Using of magnetized treated water caused an increase of about 26% in the yield. Positive significant different for the favorite of Oregano irrigated with magnetized water compared with controlled in term of major branches, water content, number of surviving seedling and chlorophyll content. A decrease in the number of clogged dripper was found under treated magnetized water compared with controlled condition.
Some Physiochemical and Microbiological Characteristics of Wastewater and Pos...IIJSRJournal
This study focuses on treating wastewater with natural materials (Charcoal and lime), identifying the physiochemical properties, identifying microorganisms in treated and untreated wastewater, and the possibility of using treated wastewater in agriculture by studying two different types of wastewater collected from two areas in Benghazi (Al-Kish and Sarti sites) in order to find out their suitability for agriculture and some other uses. The Microbiological analysis results were as follows; the growth of bacterial colonies represented in Kleibseilla pnewmonia in wastewater collected from the Sirty sites and Pseudomonas aeuroginosa & Escherichia coli from wastewater collected from the El-Kish sites, where a biological analysis of the wastewater after treating by using two types of economic and available materials in nature Calcium carbonate “lime” and Charcoal after replanting the treated wastewater with the same previous method, we obtained bacterial growth, Pseudomonas aeuroginosa in wastewater treatment by Calcium carbonate "Lime" and Escherichia coli growth in wastewater treatment by Charcoal. Wastewater treatment by Calcium carbonate "Lime" and Charcoal did not show any microbial growth, this study was illustrated there was not growth from any kind of fungi from the studied wastewater treatment and un-treatment.
Wastewater treatment was found that it is possible to benefit from the treated wastewater to irrigate green areas and insensitive crops without soil degradation, and criteria for assessing the suitability of water for irrigation according to the United States Salinity Laboratory (USSL) wastewater treatment samples are located in class C3-S1, it cannot be used to irrigate sensitive crops, especially citrus fruits, and it should only be used in lands that do not have impervious layers that prevent leaching because they need washing.
Study of Microbial Pollution in River Beehar Water District Rewa Madhya Prade...IJERA Editor
Water is one of the best gifts to all living creature, given by nature. It is compulsory for the growth and maintenance of human body and also for many biological activities. It plays a vital role for the survival of all forms of life of earth and works as a universal solvent. Pollution is caused when a change in the physical, chemical or biological condition in the environment harmfully affect quality of human life including other animal’s life and plant The quality of water is typically determined by monitoring microbial presence, especially total coliforms, fecal coliforms and fecal streptococci. The total coliform count varied in the range of 836-1987.43 MPN/100 ml, 743-981 MPN/100ml, 1115.4 to 2010 MPN/100 ml; fecal coliform varied in the range of 763-1947.61 MPN/100 ml, 547-979 MPN/100 ml and 1057 to 1378 MPN/100 ml and fecal streptococci varied in the range of 881-1969.53 and 832.63-1098.86 MPN/100 ml, 1155 to 1512 MPN/100 ml during winter, summer and rainy season, respectively.
Effect of Untreated Brewery Wastewater on the Physicochemical Properties of A...ijtsrd
The increasing rate of environmental pollution especially of water bodies due to discharges from industries has become a serious challenge. Wastewater reuse is a useful tool in minimizing the amount of effluent discharge into the environment. This research was conducted to evaluate the effect of untreated brewery effluent on the physicochemical of soil and crop yield. The physicochemical properties of untreated effluent from a brewery and its effects on soil physiochemical properties and maize crop yield, were studied using standard analytical methods. The potential of brewery effluent as a nutrient source for crop production was assessed through pot culture experiments. The effluent was found to be slightly acidic in nature, and had high BOD and COD due to the presence of large amounts of solids. The effluent was rich in nitrate, phosphate and potassium, so that its application to the soil increased the values of available nutrients in the soil. The pH of the soil decreased gradually with increasing concentration of the effluent. The brewery effluent increased the moisture content and plant nutrients of the irrigated soil. In the pot culture studies, the growth parameters such as plant height, number of leaves, root length, fresh and dry weight, number of seeds per cob and the total chlorophyll content of the maize plants were measured. The growth of the maize plant was highest with 100 untreated effluent but low in productivity, while its productivity was highest with 50 effluent. The heavy metals content of the harvested maize analysed were all within permissible limit. This research revealed that brewery wastewater reuse in agriculture at 50 is an efficient tool for pollution control as well as improved soil properties and crop yield. Mbonu, C. F. | Onuorah, S. C. | Anaukwu, C. G. "Effect of Untreated Brewery Wastewater on the Physicochemical Properties of Agricultural Soil and Crop Yield" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-4 , June 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50203.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/50203/effect-of-untreated-brewery-wastewater-on-the-physicochemical-properties-of-agricultural-soil-and-crop-yield/mbonu-c-f
Impact of wastewater irrigation on major nutrient status in soil near Bhaluka...AbdullaAlAsif1
The population increase has not only increased the fresh water demand but also increased the volume of wastewater generated. Treated or recycled wastewater (RWW) appears to be the only water resource that is increasing as other sources are dwindling. Increasing need for water has resulted in the emergence of domestic wastewater application for agriculture and its relative use. The present study was conducted at the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh during 2013 to evaluate the contribution of wastewater to major soil nutrients (N, P, K, S, Ca, Mg, B and Na) and fluctuation in physicochemical properties of soil (soil pH and Ec) from waste carrying canal at 10 selected sites of Bhaluka Upazila. Three (3) soil samples were collected at 0, 30 and 60 m distances from the waste discharging canals. The pH, EC, N, P, K, S, Ca, Mg, B and Na in soil samples decreased gradually with the increase of distance from waste discharging canal. Maximum concentrations of N at 60 and 0m distance varied from 8400 to 9700, P from 1850 to 5000, K from 4600 to 6000, S from 2000 to 4000, Ca from 7500 to 28800, Mg from 7500 to 7800, B from 90 to 2800 and Na from 2300 to 3100 μg g-1 in test soil.The results showed better nutrient status of the soil along waste discharge canals. The findings give applicable advice to commercial farmers and agricultural researchers for proper management and use of treated industrial wastewater for agricultural purpose.
Comparative Study of Test Driven Development TDD , Behavior Driven Developmen...ijtsrd
TDD, BDD and ATDD were introduced by XP Extreme Programming is an agile software development framework. They are unit testing approaches. TDD, BDD and ATDD are a software development technique which uses unit tests to incrementally deliver small pieces of functionality. TDD is a developer focused process. In Test Driven Development TDD , first come tests and then the code. The minimal piece of code is written in order to pass the designed test. In other words, it is the process of testing the code before its accrual writing. If the code passes the test, then developers can proceed to its refactoring. Behavior Driven Development BDD is a customer focused process. It is based on the full and clear understanding of the system or module behavior but in the terms of business client. The tests for TDD are created by developers for developers. The test for BDD can be written by testers or technical managers. Acceptance Test Driven Development ATDD is towards the developer focused side of things. ATDD is a technique where the entire team collaborates to define the acceptance criteria of a story before the implementation actually begins. These acceptance tests are supported necessary information. Using the Given When Then format, ATDD approach can implement. Myint Myint Moe ""Comparative Study of Test-Driven Development (TDD), Behavior-Driven Development (BDD) and Acceptance Test–Driven Development (ATDD)"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23698.pdf
Paper URL: https://www.ijtsrd.com/computer-science/other/23698/comparative-study-of-test-driven-development-tdd-behavior-driven-development-bdd-and--acceptance-test%C3%A2%E2%82%AC%E2%80%9Cdriven-development-atdd/myint-myint-moe
Investigation of Toxic Substance in Basra WatersIJCMESJOURNAL
There was a need to investigate the presence of toxic elements and compounds in drinking and desalination water for the population of the Basra city, especially after increasing cases of poisoning and diarrhea recently to more than 70,000 cases in the province of Basra according to the Human Rights Commission in the province . Six Samples (A, B, C, D, E, F) of drinking water and human uses of (RO) waters packaged in bottles produced by a private company (sample A), (RO) waters for direct sale (sample B) , waters desalination for the areas of Qibla, Jubaila and Brathaia (samples C,D,E) in addition to the waters of Shatt Al-Arab (sample F) for the purpose of conducting analysis and knowing the percentages of toxic elements and compounds in the compositions of these samples. The results of the analysis of the samples under the study confirmed that the ratio of total soluble solids (TDS) of the samples ( D, E , F ) to the other samples (A, B, C) ). The results of the analysis also confirmed the high percentage of the lead element (Pb) of the two samples (E, F) from the other samples. The results showed an increase in sulphate (SO4) for sample (E) than the other samples. The results showed an increase in the ratio of phosphate (PO4) of sample (F) to the rest of the samples in comparison with the standards of the World Health Organization (WHO). These results indicate that pollution in the Shatt Al-Arab waters increased with toxic elements and compounds. These results correspond to the statistics of the Directorate of Basra Heaith, where it showed the increase in the proportion of casualties among the population of the province of Basra to areas adjacent to the Shatt Al-Arab river, such as the city center , Abu- Khasseb and Al-Tanuma from the rest of the areas causing water pollution and the spread of E. coli causing diarrhea and intestinal colic with 50% of cases which was admitted to Basra hospitals.
Spearmint (Mentha spicata L.) Response to Deficit Irrigationpaperpublications3
Abstract: An experiment was conducted with the objective to identify the level of deficit irrigation which allows achieving optimal yield and investigate the effect of deficit irrigation practice on spearmint (Mentha Spicata L.) yield and yield components. The experiment was conducted at Koka research station of Wondo Genet Agricultural Research Center, Ethiopia latitude 8O26’, longitude 39O2’ and altitude 1602masl for two years (2011/2012 and 2012/2013 dry season). Nine treatments three level of irrigation water amount percentage based on evapo-transpiration of the crop (ETc) (100%ETc, 75%ETc and 50%ETc) and three types of furrow irrigation water application method (alternate furrow, fixed furrow and conventional furrow) were used in randomized completely block design (RCBD) with three replications. The pooled mean of two year data showed different level of deficit irrigation had a significant effect (p<0.05)><0.01) variation among treatments due to deficit irrigation on dry biomass yield per hectare, dry leaf yield per hectare, essential oil yield per hectare and water use efficiency. However, different deficit irrigation level had no significant influence on wet harvesting index. The highest fresh biomass, dry biomass, fresh leaf, dry leaf and essential oil yield per harvesting cycle of 12093kg/ha, 3746kg/ha, 8133kg/ha, 2441.1kg/ha and 37.0kg/ha respectively was obtained due to 100%ETc with conventional furrow application method. Moreover, the highest water use efficiency of 16.3x10-3kg/m3 was achieved due to deficit irrigation to 50%ETc with alternate furrow irrigation water application method. The study showed that the best treatment is deficit irrigation to 50%ETc with conventional furrow application method which had no significant variation with treatments that showed higher yields of spearmint including water use efficiency.
Keywords: Alternate furrow, conventional furrow, deficit irrigation, fixed furrow, spearmint.
Water Pollution is an undesirable change in physical, chemical or biological characteristics of water. Human population explosion, rapid industrialization, deforestation, unplanned urbanization, scientific and technological advancement etc. are mainly responsible for the pollution crisis on the earth.
Wastewater from manufacturing or chemical processes in industries contributes to water pollution. Industrial wastewater usually contains specific and readily identifiable chemical compounds.
During the last fifty years, the number of industries in India has grown rapidly. But water pollution is concentrated within a few sub sectors, mainly in the form of toxic wastes and organic pollutants. Out of this a large portion can be traced to the processing of industrial chemicals and to the food products industry.
The effects of water pollution are not only devastating to people but also to animals, fish, and birds. Polluted water is unsuitable for drinking, recreation, agriculture, and industry. It diminishes the aesthetic quality of lakes and rivers. More seriously, contaminated water destroys aquatic life and reduces its reproductive ability. Eventually, it is a hazard to human health. Nobody can escape the effects of water pollution.
Most major industries have treatment facilities for industrial effluents. But this is not the case with small-scale industries, which cannot afford enormous investments in pollution control equipment, as their profit margin is very slender. So the present study may be helpful for finding out methods for the treatment of effluents in batch scale that are cost effective so that small-scale industries can treat their wastes before releasing them into the environment.
The Effects of Using Magnetic Treatment Brackish Water in Irrigation on the Y...Agriculture Journal IJOEAR
Abstract— Over abstraction of groundwater and limitation of natural recharge in the Lower Jordan Valley are causing an increase in groundwater salinity. The high demand for medicine herbs at international market attracted Palestinian farmers to grow medicine herbs including Oregano. Pilot project was carried out under greenhouse condition where magnetize technology introduced for the first time in irrigation of Oregano to overcome salinity problem. Using of magnetized treated water caused an increase of about 26% in the yield. Positive significant different for the favorite of Oregano irrigated with magnetized water compared with controlled in term of major branches, water content, number of surviving seedling and chlorophyll content. A decrease in the number of clogged dripper was found under treated magnetized water compared with controlled condition.
Some Physiochemical and Microbiological Characteristics of Wastewater and Pos...IIJSRJournal
This study focuses on treating wastewater with natural materials (Charcoal and lime), identifying the physiochemical properties, identifying microorganisms in treated and untreated wastewater, and the possibility of using treated wastewater in agriculture by studying two different types of wastewater collected from two areas in Benghazi (Al-Kish and Sarti sites) in order to find out their suitability for agriculture and some other uses. The Microbiological analysis results were as follows; the growth of bacterial colonies represented in Kleibseilla pnewmonia in wastewater collected from the Sirty sites and Pseudomonas aeuroginosa & Escherichia coli from wastewater collected from the El-Kish sites, where a biological analysis of the wastewater after treating by using two types of economic and available materials in nature Calcium carbonate “lime” and Charcoal after replanting the treated wastewater with the same previous method, we obtained bacterial growth, Pseudomonas aeuroginosa in wastewater treatment by Calcium carbonate "Lime" and Escherichia coli growth in wastewater treatment by Charcoal. Wastewater treatment by Calcium carbonate "Lime" and Charcoal did not show any microbial growth, this study was illustrated there was not growth from any kind of fungi from the studied wastewater treatment and un-treatment.
Wastewater treatment was found that it is possible to benefit from the treated wastewater to irrigate green areas and insensitive crops without soil degradation, and criteria for assessing the suitability of water for irrigation according to the United States Salinity Laboratory (USSL) wastewater treatment samples are located in class C3-S1, it cannot be used to irrigate sensitive crops, especially citrus fruits, and it should only be used in lands that do not have impervious layers that prevent leaching because they need washing.
Study of Microbial Pollution in River Beehar Water District Rewa Madhya Prade...IJERA Editor
Water is one of the best gifts to all living creature, given by nature. It is compulsory for the growth and maintenance of human body and also for many biological activities. It plays a vital role for the survival of all forms of life of earth and works as a universal solvent. Pollution is caused when a change in the physical, chemical or biological condition in the environment harmfully affect quality of human life including other animal’s life and plant The quality of water is typically determined by monitoring microbial presence, especially total coliforms, fecal coliforms and fecal streptococci. The total coliform count varied in the range of 836-1987.43 MPN/100 ml, 743-981 MPN/100ml, 1115.4 to 2010 MPN/100 ml; fecal coliform varied in the range of 763-1947.61 MPN/100 ml, 547-979 MPN/100 ml and 1057 to 1378 MPN/100 ml and fecal streptococci varied in the range of 881-1969.53 and 832.63-1098.86 MPN/100 ml, 1155 to 1512 MPN/100 ml during winter, summer and rainy season, respectively.
Effect of Untreated Brewery Wastewater on the Physicochemical Properties of A...ijtsrd
The increasing rate of environmental pollution especially of water bodies due to discharges from industries has become a serious challenge. Wastewater reuse is a useful tool in minimizing the amount of effluent discharge into the environment. This research was conducted to evaluate the effect of untreated brewery effluent on the physicochemical of soil and crop yield. The physicochemical properties of untreated effluent from a brewery and its effects on soil physiochemical properties and maize crop yield, were studied using standard analytical methods. The potential of brewery effluent as a nutrient source for crop production was assessed through pot culture experiments. The effluent was found to be slightly acidic in nature, and had high BOD and COD due to the presence of large amounts of solids. The effluent was rich in nitrate, phosphate and potassium, so that its application to the soil increased the values of available nutrients in the soil. The pH of the soil decreased gradually with increasing concentration of the effluent. The brewery effluent increased the moisture content and plant nutrients of the irrigated soil. In the pot culture studies, the growth parameters such as plant height, number of leaves, root length, fresh and dry weight, number of seeds per cob and the total chlorophyll content of the maize plants were measured. The growth of the maize plant was highest with 100 untreated effluent but low in productivity, while its productivity was highest with 50 effluent. The heavy metals content of the harvested maize analysed were all within permissible limit. This research revealed that brewery wastewater reuse in agriculture at 50 is an efficient tool for pollution control as well as improved soil properties and crop yield. Mbonu, C. F. | Onuorah, S. C. | Anaukwu, C. G. "Effect of Untreated Brewery Wastewater on the Physicochemical Properties of Agricultural Soil and Crop Yield" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-4 , June 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50203.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/50203/effect-of-untreated-brewery-wastewater-on-the-physicochemical-properties-of-agricultural-soil-and-crop-yield/mbonu-c-f
Impact of wastewater irrigation on major nutrient status in soil near Bhaluka...AbdullaAlAsif1
The population increase has not only increased the fresh water demand but also increased the volume of wastewater generated. Treated or recycled wastewater (RWW) appears to be the only water resource that is increasing as other sources are dwindling. Increasing need for water has resulted in the emergence of domestic wastewater application for agriculture and its relative use. The present study was conducted at the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh during 2013 to evaluate the contribution of wastewater to major soil nutrients (N, P, K, S, Ca, Mg, B and Na) and fluctuation in physicochemical properties of soil (soil pH and Ec) from waste carrying canal at 10 selected sites of Bhaluka Upazila. Three (3) soil samples were collected at 0, 30 and 60 m distances from the waste discharging canals. The pH, EC, N, P, K, S, Ca, Mg, B and Na in soil samples decreased gradually with the increase of distance from waste discharging canal. Maximum concentrations of N at 60 and 0m distance varied from 8400 to 9700, P from 1850 to 5000, K from 4600 to 6000, S from 2000 to 4000, Ca from 7500 to 28800, Mg from 7500 to 7800, B from 90 to 2800 and Na from 2300 to 3100 μg g-1 in test soil.The results showed better nutrient status of the soil along waste discharge canals. The findings give applicable advice to commercial farmers and agricultural researchers for proper management and use of treated industrial wastewater for agricultural purpose.
Spearmint (Mentha spicata L.) Response to Deficit Irrigationpaperpublications3
Abstract: An experiment was conducted with the objective to identify the level of deficit irrigation which allows achieving optimal yield and investigate the effect of deficit irrigation practice on spearmint (Mentha Spicata L.) yield and yield components. The experiment was conducted at Koka research station of Wondo Genet Agricultural Research Center, Ethiopia latitude 8O26’, longitude 39O2’ and altitude 1602masl for two years (2011/2012 and 2012/2013 dry season). Nine treatments three level of irrigation water amount percentage based on evapo-transpiration of the crop (ETc) (100%ETc, 75%ETc and 50%ETc) and three types of furrow irrigation water application method (alternate furrow, fixed furrow and conventional furrow) were used in randomized completely block design (RCBD) with three replications. The pooled mean of two year data showed different level of deficit irrigation had a significant effect (p<0.05)><0.01) variation among treatments due to deficit irrigation on dry biomass yield per hectare, dry leaf yield per hectare, essential oil yield per hectare and water use efficiency. However, different deficit irrigation level had no significant influence on wet harvesting index. The highest fresh biomass, dry biomass, fresh leaf, dry leaf and essential oil yield per harvesting cycle of 12093kg/ha, 3746kg/ha, 8133kg/ha, 2441.1kg/ha and 37.0kg/ha respectively was obtained due to 100%ETc with conventional furrow application method. Moreover, the highest water use efficiency of 16.3x10-3kg/m3 was achieved due to deficit irrigation to 50%ETc with alternate furrow irrigation water application method. The study showed that the best treatment is deficit irrigation to 50%ETc with conventional furrow application method which had no significant variation with treatments that showed higher yields of spearmint including water use efficiency.
Characterization of Physicochemical Water Quality Parameters of River Gudar (...IOSRJAC
Due to various natural and anthropogenic activities, quality of water was deteriorated in most towns of the country. These changes make or forced the community to depend on unsafe and poor water consumption. River Gudar is one of the rivers found in towns of the country, and exposed to many anthropogenic activities done around the town. Importance of river Gudar is strongly linked to Domestic consumption, Irrigation, Industrial (HAEF) and other public health. Under the investigation physicochemical parameters and selected heavy metals for the quality of river Gudar for drinking purpose were characterized. The laboratory results for both physicochemical parameters and selected heavy metals were: Alkalinity (154 ± 15.556 mg/L), pH (8.44), Electrical Conductivity (316.47 ± 72.802 Scm-1 ), TDS (149.37 ± 20.64 mg/L), Ammonia (41.00 ± 1.19 mg/L), Phosphate (3.50 ± 0.32mg/L), Total Hardness (156.87 ± 8.46 mg/L CaCO3), Sodium (17.44 ± 2.87mg/L), Potassium (8.51 ± 0.33 mg/L) and some selected heavy metals such as Cu (0.29 ± 0.04 mg/L ), Pb (2.5 ± 0.25 mg/L), Zn (0.63 ± 0.24 mg/L), Cd (0.79 ± 0.19 mg/L) and Ag (0.35 ± 0.17 mg/L) for water sample.
Abstract— The aim of the present investigation was to assess the suitability of textile mill effluent (TME) (untreated) at different concentrations (Control, 2.5, 5, 15, 25, 50 75, and 100%) for irrigation purposes. Effect of textile mill effluent on seed germination, shoot and root length, fresh and dry weight, number of leaves, total leaf area, number of root nodules and pigments of chlorophyll ‘a’, chlorophyll ‘b’ and total chlorophyll of cow pea was studied at 15th DAS of seedlings. All morphological growth parameters, pigment contents, were found to increase at 5% textile mill effluent concentration and it decreased from 10% effluent concentration onwards. So these results reflect that the textile mill effluent is toxic to crop and it can be used for irrigation purpose after a proper treatment with appropriate dilution.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
1. International Journal of Scientific and Research Publications, Volume 9, Issue 3, March 2019 242
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http://dx.doi.org/10.29322/IJSRP.9.03.2019.p8739 www.ijsrp.org
Effects of Tannery Effluent on Seed Germination and
Growth Performance of Selected Varieties of
Maize (Zea mays L.)
Minde Hailu*, Manikandan Muthuswamy **, Yohannes Petros**
*Department of Biology, Debark University, Gondar, Ethiopia
**School of Biological Sciences and Biotechnology, Haramaya University, P.O.Box.138, Dire Dawa, Ethiopia
Correspondence Author – Minde Hailu, Email- mindhailu@gmail.com, Cell phone- +25124611304
DOI: 10.29322/IJSRP.9.03.2019.p8739
http://dx.doi.org/10.29322/IJSRP.9.03.2019.p8739
Abstract: Industrial effluents with heavy metals adversely affect the growth and development of crops when it is used for irrigation.
So this study was carried out to assess the effect of tannery effluent on seed germination and growth performance of selected varieties
(BHQPY545 and Malkesa-2) of Maize using different effluent treatments. The treatments were made by mixing measured amount of
tannery effluent in distilled water i.e. 0, 25, 50, 75 and 100%. The experiment was carried out in Completely Randomized Design
with five effluent treatments, each replicated three times. The effluent is dark bluish green in colour with high biological oxygen
demand(182mg/kg) along with much higher concentrations of total suspended solids (13,100 mg/kg) and total dissolved solids (3000
mg/kg) but concentration of some metals were found under normal range recommended by Food and Agriculture Organization and
United Nations Environmental Protection Agency. Tannery effluent was tested for its effect on maize in laboratory and greenhouse
conditions. The result showed that a maximum reduction 26.67% and 33.33% in germination percentage, 81.69% and 75.69% in
radicle length and 64.79% and 75% in plumule length was observed in BHQPY545 and Malkesa-2 respectively at 100% tannery
effluent treatments. Vegetative growth parameters like shoot length, root length and number of leaves per plant were reduced with
increasing levels of effluent treatments. Yield parameters of plant were also reduced due to increase in level of effluent treatments.
Generally the effects of tannery effluent on germination and growth performance showed that effluent was not fit for irrigation due to
higher concentration of organic and in organic matter and heavy metal contents. It is concluded that tannery effluent can’t be used for
irrigation purpose unless properly treated and diluted.
Index Terms- Germination percentage, Plumule, Radicle, Vegetative growth, Yield
I. INTRODUCTION
ater pollution is one of the most serious problems due to
the addition of large amounts of waste materials to the
water bodies. Especially, most of the industries dump their
toxic effluents and pollute different water bodies such as
lakes, rivers, oceans and ground water. These effluents contain
toxic organic and inorganic suspended or dissolved solids,
which have adverse effects on environment and human health
(Begum et al., 2010). Industries discharge a variety of
pollutants in their wastewater including heavy metals, resin
pellets, organic toxins, oils, nutrient and solids. These
pollutants cause the contamination of soil, water and air,
which are associated with many diseases (Huma et al., 2012).
The effluent discharging industries are distilleries, sugar mills,
pulp and paper mills, detergent, chemical factories, textile
dyeing industries, tanneries, electroplating, pharmaceuticals
and dairy industries. Among these, tanneries play a major role
in creating serious pollution problems than other industries.
The release of effluents without proper treatment into the
nearby rivers, irrigation canals and streams adjacent to
agricultural fields cause serious hazards intensifying the
adverse effects on ecosystems like water, soils and plants
(Shukry, 2001 )
Tannery is one of the major foreign currencies earning
industry in Ethiopia. Consequently, during the past 20 years
the industries had significant government support and increase
the number throughout the country. Moreover, tanning
industry involves chemical reactions and mechanical changes
which use a lot of water. It generates waste most of the time
which are, in developing countries discharged to rivers or
other water areas or to open field land. It could have adverse
effect on the environment and human health if it is not
properly managed due to the presence of dangerous chemical
elements such as chromium, sulfur, etc. (Favazzi, 2003).
Moreover, the river nearby the tanneries will be polluted since
they are serving as recipient of effluent from the factories. In
Ethiopia, Akaki Kaliti and Modjo rivers that are tributaries of
Awash River are best examples of polluted water bodies by
tanneries. The residents around the river and /or the tannery
reported the death of their cattle, drying up of green plants,
waterborne diseases and bad smell that resulted due to the
W
2. International Journal of Scientific and Research Publications, Volume 9, Issue 3, March 2019 243
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death of organisms and action of microorganisms that in turn
caused by depletion of dissolved oxygen are due to tannery
effluent. With the ever increasing demand for irrigation water
supply, farmlands close to tanneries are frequently irrigated
with poor quality water (Eskindir, 2002). In several arid and
semiarid regions, water shortage is limiting factor, the farmers
nearby tannery industries are forced to use the tannery
effluents for irrigation purpose. In this scenario, the alternative
technique is the use of diluted effluent for irrigation. This not
only solves its disposal problem, but also will serve as natural
fertilizer for several crops if it is used at proper concentration
(Rajendra et al., 2010). The effect of various industrial
effluent and metal elements on seed germination and growth
and yield of crop plants have gained the attention of many
researchers (Rahman, et al., 2002). However, the effect of
tannery effluent on Maize crop plants has not been reported in
Ethiopia.
II. MATERIALS AND METHODS
2.1. Description of the Study Area
The laboratory experiment was conducted in the Botanical
Science Laboratory, School of Biological Sciences and
Biotechnology, Haramaya University, Ethiopia. The
greenhouse experiment was conducted at Haramaya
University greenhouse stations Rarre. The station at Rarre is
1980m above sea level and located at latitude of 9° 26 ' N and
longitude 42° 3 ' E. It is situated in the semi-arid tropical belt
of eastern Ethiopia. The mean annual rainfall, as recorded
from meteorology station located in Haramaya University,
received ranges from 600 to 1260 mm and is bimodal. The
short rainy season usually starts in March and extends to May,
while the main/long rainy season stretches from end of June to
September. Minimum and maximum annual temperatures
range from 6 to 12 and 17 to 25 0
C, respectively. The soil of
the experimental site is classified as fluvisol. The major food
crops grown are cereals mainly maize and sorghum (Kibebew,
2014).
2.2. Description of effluent Sampling Site
The sample was collected from Modjo tanning industry, which
is found in Modjo town and located 80 km south of Addis
Ababa. It is a medium-sized leather industry in Ethiopia with
installed capacity of processing 844,000 and 1, 656,000 sheep
and goat skins, respectively, per annum. The volume of
wastewater discharged into the Modjo River varies between
3500-5500 cubic meters per day. Apart from its unfortunate
use as open waste disposal site the river is also used as a
source of irrigation for the rural population living around the
Modjo River (Amanial, 2015).
Figure 1: Map of sample site (Lume Woreda, Oromia region)
2.3. Plant Materials
The selection of the crop plant was based on its economic
importance as well as availability in Haramaya University,
Ethiopia. Accordingly, two available varieties of Maize
BHQPY545 and Malkesa-2 were used in this study.
2.4. Experimental Design
The study involved laboratory as well as greenhouse
experiments. The laboratory study was based on bioassay in
order to assess effect of tannery effluent on seed germination
percentage and seedling growth. The greenhouse experiment
was mainly focused on identifying the effect of tannery
effluent on growth performance of selected varieties of Maize
BHQPY545 and Malkesa-2. The experimental design is based
on completely randomized design (CRD) with three
replications for each treatment and control.
2.5. Collection and Preparation of Effluent for Treatments
The effluents were collected in plastic containers directly from
the outlet of Modjo tannery in East Showa, Oromia region,
Ethiopia and prepared in different treatments. Different
treatments of tannery effluent were made by mixing with
distilled water with following percentage.
T0 = 100% distilled water (control)
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T1 = 25% effluent + 75% distilled water
T2 = 50% effluent + 50% distilled water
T3 = 75% effluent + 25% distilled water
T4 = 100% effluent
Additionally, two liters of effluent were collected and brought
to central laboratory, Haramaya University, Ethiopia for
different chemical analyses. The effluent was filtered through
whtaman No. 1 filter paper to remove solids and stored at 4°C.
2.6. Laboratory Examination
2.6.1. Determination of Physicochemical Parameters
The raw or untreated tannery effluent was analyzed for various
physicochemical properties by standard method of analysis as
per the guidelines of American Public Health Association
(APHA, 1998). PH was determined by pH meter, EC
(Electrical conductivity) by EC meter, TDS (Total dissolved
solids) by gravimetric method, BOD (Biological oxygen
demand) by dissolved oxygen meter (incubator), and AAS
(atomic absorption spectrophotometer) was used to determine
heavy metals such as Cd, Cr, Cu, Fe, Pb, and Zn.
To determine the presence of heavy metals in the collected
effluent samples 100ml of each of the effluent samples were
filtered using Whatman No. 1 filter paper then transferred into
beakers containing 10ml of concentrated HNO3. The samples
were boiled slowly and then evaporated on a hot plate to the
lowest possible volume (about 20ml). The beakers were
allowed to cool and another 5ml of concentrated HNO3 was
added. Heating was continuous with the addition of
concentrated HNO3 as necessary until digestion was complete.
The samples were evaporated again to dryness (but not baked)
and the beakers were cooled, followed by the addition of 5ml
of HCl solution. The solutions were then warmed and 5ml of
NaOH was added, then filtered. The filtrates were transferred
to 100ml volumetric flasks and diluted to the mark with
distilled water. The solutions were then used for elemental
analysis. A total of six metallic elements Cd, Cr, Cu, Fe, Pb
and Zn were determined.
2.6.2. Seed germination test
The bioassay studies were carried out following the method of
Heisey (1990). Seeds were surface sterilized with antifungal
15% sodium hypochlorite for two minutes and then washed
with distilled water for several times to remove the sodium
hypochlorite. Five surface sterilized seeds were placed on
whatman No. 1 filter paper 9cm diameter petridishes and the
petriplate were arranged on the table following CRD with
three replications. Each petriplate was moistened with 2ml
tannery effluent with different concentration (25%, 50%, 75%
and 100%) and the control was treated with distilled water and
incubated at room temperature. The moisture of petriplate was
maintained by adding 2ml of respective tannery effluent in
each consecutive day.
Germination percentage, plumule and radical length were
measured after 6 days of incubation. The germination
percentage was calculated by using the following formula.
Germination (%) =
2.7. Green House Experiments
Seeds were surface sterilized with antifungal 15% sodium
hypochlorite for two minutes and then washed with distilled
water for several times to remove the sodium hypochlorite.
Three surface sterilized seeds were sown in plastic pots that
filled with equal amount of soil (1:1:1 garden soil, cow dung
manure and sand); in green house using CRD design with
three replications along with their respective controls. The
experimental plants were treated with 1000 ml of respective
tannery effluent (25%, 50%, 75% and 100%) every 3 days
after 5th leaf emergence (17th day after sowing) and the
control was treated with normal water. All the plants were
grown under the greenhouse condition in Haramaya
University.
Observations on morphological parameters like shoot length,
root length, number of leaves, fresh weight and dry weight of
shoot and root and yield parameters like ear per plant, seed
number per plant, seed weight per ear and 100seed weight per
ear were recorded at the end of the experiment after five
months of sowing.
Root and shoot length: - Three plants were taken from each
pots and their shoot and root lengths in cm were measured by
using a measuring ruler and the values were recorded. Since
the maize root was fibrous, the largest root was used for
measurement.
Fresh and dry weight: - Three plants were collected from
each pot and their fresh weights in mg were measured with the
help of an electrical single pan balance. Their dry weights in
mg were taken after keeping them in a hot air oven at 80 0
C
for 24 hours by using an electrical single pan balance.
III. RESULTS AND DISCUSSION
3.1 Physicochemical Characteristics of Tannery Effluent
The physicochemical parameters such as color, odor, pH,
BOD, EC, total dissolved solids, total suspended solid and
total solid were analyzed and the results are indicated in table
1 with maximum concentration recommended for crop
production (FAO, 1985 and USEPA, 2012).
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Table 1: Physicochemical characteristics of tannery effluent
Parameters Unit Values Standard set for crop production
Colour - Dark bluish green -
Odour - Pungent (offensive) -
pH - 5.88 6-9
BOD mg/L 182 <30
EC μS/cm 13,090 <3,000
TDS mg/L 13,100 <2000
TSS mg/L 3,000 <30
TS mg/L 16,100 -
The tannery effluent used in this study was dark bluish green
in colour. This is may be due to presence of high TSS and
TDS. Kambole (2003) reported that high colour detected could
be attributed due to the high TSS and TDS. The dark bluish
green color of the effluent is the major problem to the natural
environment. It reduces light penetration in water bodies
which in turn affect the aquatic life (Pazouki et al., 2008). The
odour of the effluent was pungent. The offensive odour of
effluent is due to reduction of sulphate compounds to
hydrogen sulphide by sulphate-reducing bacteria (Mahimaraja
and Bolan, 2004). The effluent was acidic in nature with pH
recorded as 5.88. Kohli and Malaviya (2013) reported that raw
tannery effluent was acidic with pH of 3.61. However, a report
from different investigation has shown the basic nature of
tannery effluents (Aklilu et al., 2012; Mandakini and Khillare,
2016). The acidic pH of the tannery effluent may be due to
the presence of high concentration of chrome, which is in line
with Reddy et al. (2014) who reported that chrome effluent is
highly acidic in nature. The pH obtained was lower than that
of the permissible level for crop production (FAO, 1985;
USEPA, 2012).
The effluent contains much higher amount of total dissolved
solid, suspended solid, electrical conductivity and BOD than
that of the limit prescribed by FAO (1985) and USEPA
(2012). The electrical conductivity value of the tannery
effluent recorded was 13090μS/cm. Similarly, higher electrical
conductivity of tannery effluent was reported by Aklilu et al.
(2012). The higher electrical conductivity of the effluent is
due to presence of high concentration of dissolved salts in the
effluent (Bhasin et al., 2007).
Determination of BOD is one of the important parameters
used in water pollution to evaluate the impact of wastewaters
on receiving water bodies. The value obtained for BOD was
182 mg/L. Aklilu et al. (2012) also reported higher amount of
BOD (147.29 mg/L) which is lower than the present report. In
contrast to this study, Zereen et al. (2013) reported much
higher amount BOD which was 987 mg/L. Increase in BOD
which is a reflection of microbial oxygen demand leads to
depletion of DO which may cause hypoxia conditions with
consequent adverse effects on aquatic biota (An et al., 2006).
High values of BOD might be due to presence of higher
amounts of organic compounds in the effluents (Bhasin et al.,
2007).
The result obtained for TDS and TSS of effluent were 13100
and 3000 mg/L respectively. Kohli and Malaviya (2013) also
reported higher amount of TSS (1580 mg/L) which was lower
than the recent study. However, Zereen et al. (2013) reported
much higher amount of TDS and TSS which was 85500mg/L
and 2150 mg/L respectively. The presence of high level of
TSS and TDS may be due to the soluble and insoluble organic
and inorganics present in the effluent (Nagarajan et al., 2005).
The result of effluent analysis for those parameters shows
conformity with the other studies (Aklilu et al., 2012 and
Mandakini and khillare, 2016).
3.2 Heavy Metal Analysis in Tannery Effluent
The heavy metal concentration of 100% (untreated) tannery
effluent was presented in Table 2 and the results were
compared to the standard prescribed for crop production
(FAO, 1985 and USEPA, 2012). The result of study indicates
that chromium had the highest concentrations in the tannery
effluent, while Zn had the least concentrations.
The metal concentrations in effluent samples were in the order
of Cr > Pb > Fe > Cd > Cu > Zn. The concentrations of heavy
metals such as Cd, Cu and Cr in the tannery effluent were
above the maximum concentration recommended for crop
production, while the concentration Fe, Pb and Zn are within
normal range recommended by FAO (1985) and USEPA
(2012). Moreover, the heavy metals such as copper, zinc, iron
and chromium are essential for biochemical and physiological
function of plants and animals at trace amount (Nagajyoti et
al., 2010). Higher concentration of heavy metals poses an
adverse effect to plant as well as animals. Several researchers
reported the effect of heavy metals on plant. It has been
revealed that high concentration of heavy metals affects the
plant by inhibit the seed germination, seedling growth, root
growth, shoot growth and plant biomass (Aklilu et al., 2012;
Zereen et al., 2013; Mandakini and Khillare, 2016)
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Table 2: Heavy metal concentrations in tannery effluent
Heavy metals Unit Concentration in effluent Standard set for crop production
Cadmium, Cd mg/L 0.33 0.01
Copper, Cu mg/L 0.22 0.20
Chromium, Cr mg/L 2.74 0.10
Iron, Fe mg/L 1.38 5
Lead, Pb mg/L 1.50 5
Zinc, Zn mg/L 0.12 2
3.3. Effects of Tannery Effluent on Seed Germination
Percentage
Impacts of different concentration of tannery effluents on seed
germination of two selected varieties of Maize (Zea mays)
were indicated in table 3. The seed germination percentage
was decreased with increasing concentration of effluent. A
maximum of 26.67% and 33.33% reduction in germination
percentage was observed in BHQPY545 and Malkesa-2
varieties respectively at 100% tannery effluent treatments.
Among the selected varieties, BHQPY545 showed better
performance in seed germination percentage as compared to
Malkesa-2. Inhibition of seed germination at higher
concentrations of the effluent may be due to high levels of
dissolved solids which enhance the salinity and conductivity
of absorbed solute by the seeds (Sundaramoorthy and
Kunjithapatham, 2000).
Moreover, similar observations were reported by Mandakini
and Khillare (2016) for seed germination in tomato, Aklilu et
al. (2012) for seed germination in selected vegetables, and
Kohli and Malaviya (2013) for seed germination in wheat
treated with concentration of tannery effluents.
Table 3: Effects of tannery effluent on the germination potential of maize
Maize Varieties Treatments Germination %
3 Day* 4 Day* 5 Day* 6 Day*
BHQPY545
T0(Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
53.33±6.67a
33.33± 6.67b
13.33 ±6.67c
6.67±6.67c
0.00±00
93.33a±6.67a
60.00±11.55b
46.67±6.67bc
26.67±6.67cd
6.67±6.67d
100.00±0.00a
73.33± 6.67b
66.67±6.67bc
53.33±6.67cd
46.67±6.67d
100.00±0.00a
93.33±6.67ab
86.67±6.67ab
80.00±11.55ab
73.33±6.67b
Malkes2
T0(Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
46.67± 6.67a
33.33 ± 6.67a
13.33 ±6.67b
0.00±0.00
0.00±0.00
73.33±13.3a
60.00±0.00b
33.33±6.67b
20.00±0.00bc
6.67±6.67c
100.00 ±0.00a
80.00±0.00ab
60.00±11.55bc
46.67± 6.67c
40.00±11.55c
100.00 ±0.00a
86.67±6.67ab
80.00±11.55ab
73.33 ±6.67b
66.67 ±6.67b
Mean followed by different letters in the same column are significantly different at p=0.05 according to Duncan’s new multiple range test, ± = Standard error, * Days
after sowing. T0 = 100% distilled water (control), T1 = 25% effluent + 75% distilled water, T2 = 50% effluent + 50% distilled water, T3 = 75% effluent + 25% distilled
water, T4 = 100% effluent
3.4. Effects of Tannery Effluent on Radicle and Plumule
Length
The data presented in Table 4 shows, the effects of tannery
effluents on radicle and plumule length of selected varieties of
Maize (Zea mays). The result showed that radicle and plumule
length was decreased with increasing concentrations of
effluent treatment. A maximum of 81.69% and 75.69%
reduction in radicle length and 64.79% and 75% reduction in
plumule length was observed in BHQPY545 and Malkesa-2
respectively at 100% tannery effluent treatments.
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Table 4: Effects of tannery effluent on plumule and radicle length of maize
Maize Varieties Treatments Plumule Length Radicle Length
BHQPY545
T0 ( Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
1.42± 0.3a
1.37± 0.23a
0.72± 0.03b
0.69 ± 0.07b
0.50 ± 0.09b
5.79 ± 0.48a
2.29 ± 0.27b
1.71 ± 0.36bc
1.63 ± 0.36bc
1.06 ±0.19c
Malkesa-2
T0 ( Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
2.04 ±0.2139a
1.03 ±0.1507b
0.97 ±0.1122bc
0.73 ± 0.18bc
0.51 ±0.06c
4.69 ±0.10a
2.49 ±0.16b
2.12 ±0.26bc
1.72 ±0.28cd
1.14 ±0.10d
Mean followed by different letters in the same column are significantly different at p=0.05 according to Duncan’s new multiple range test. ± Standard error, T0 = 100%
distilled water (control), T1 = 25% effluent + 75% distilled water, T2 = 50% effluent + 50% distilled water, T3 = 75% effluent + 25% distilled water, T4 = 100%
effluent
Among these two varieties, at the lowest concentration radicle
and plumule growth was also inhibited as compared with
control groups. But the inhibitory effect was less as compared
with highest concentration of effluent treatment.
3.5. Effects of Tannery Effluent on Vegetative Growth and
Biomass
The effects of tannery effluents on vegetative growth
performance and biomass were observed and the results were
presented in table 5. Increasing levels of effluents treatments
caused reduction in all the selected parameters of vegetative
growth and biomass in the selected varieties of maize
(BHQPY-545 and Malkesa-2) as compared to control groups.
The morphological appearance of plants from the control and
T1 treatments of all the two selected varieties were healthier,
lush green with well-developed and expanded leaves as
compared to T2, T3 and T4 treatments. The comparison of
leaf number in the treatments and control groups, showed that
mean values significantly (P = 0.05) decreased with increasing
the concentration as compared with control groups in both
varieties. The inhibitory effect on leaf number was highly
significant in T3 and T4 concentration when compared with
control. However, in the lowest concentration (T1) inhibitory
effect on leaf number was not significantly (p = 0.05) different
as compared with control groups. The maximum (165.42) and
minimum (138.62cm) shoot length was observed in T1 and T4
treated plants in BHQPY-545 varieties as compared to control
groups. The inhibitory effect was highly significant at highest
levels of concentration as compared to control and lowest
concentration. Moreover, shoot length range was observed
from 163.71 to 93.69 cm in malkesa-2 varieties. But the
inhibitory effect on shoot length was higher as compared to
BHQPY-545 varieties.
Root growth was also observed; it showed the ranges from
37.11 to 31.06 cm and 37.74 to 26.56cm for BHQPY-545 and
Malkesa-2 varieties respectively. However, a maximum of
25.84% and 45.81% reduction in shoot length and 43.53% and
39.99% reduction in root length was observed in BHQPY545
and Malkesa-2 respectively at 100% tannery effluent
treatments. The results are in agreement with the finding of
Zereen et al. (2013) who reported that tannery effluent
adversely affected the root and shoot development of all the
cultivars of sunflower in higher treatment concentration as
compared to controls groups.
Finally, shoot and root biomass fresh and dry weight were also
recorded and indicated in table 5. It indicates both root and
shoots biomass weight decreased with increasing the
concentrations of effluent treatment. The drastic changes in
the shoot and root biomass weight were observed at highest
concentration (T4) and the effect was significant (P = 0.05)
compared to control and T1.
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Table 5: Effects of tannery effluent on vegetative growth and biomass of maize
Mean followed by different letters in the same column are significantly different at p=0.05 according to Duncan’s new multiple range test, ± = Standard error, Tr = treatments, T0 = 100% tap water (control), T1 =
25% effluent + 75% tap water, T2 = 50% effluent + 50% tap water, T3 = 75% effluent + 25% tap water, T4 = 100% effluent
Maize
variety
Tr. No. of leaves Shoot length Root length Fresh weight Dry weight
Shoot Root Shoot Root
BHQPY-
545
T0
T1
T2
T3
T4
14.32±0.19a
13.66± 0.20a
13.10±0.10a
11.76±0.79b
10.53±0.23b
186.93±17.06a
165.42±10.03ab
151.31±2.70b
139.28±1.60b
138.62±4.37b
55.00±0.51a
37.11±0.40b
32.55±0.40c
31.56±0.99c
31.06±0.53c
498.48±46.40a
311.78±28.87b
181.75± 6.98c
150.6± 3.80c
120.49± 4.21c
214.85±13.19a
120.09 ±2.83b
92.15± 2.39c
54.72± 5.63d
33.70 ±1.81d
290.09±27.67a
183.78±36.47b
109.93±5.72c
102.90±2.37c
69.59±3.21c
147.12±19.37a
92.45±4.64b
62.21±4.37c
27.65±5.72d
23.61±2.98d
Malkesa-2
T0
T1
T2
T3
T4
17.00±0.57a
15.32±0.50ab
13.43±0.72bc
12.66±0.50cd
10.66±0.84d
172.89±4.52a
163.71±0.63a
134.11±1.28b
117.15±1.85b
93.69±12.66c
44.26±0.49a
37.74±0.26b
33.67±0.38c
27.94±0.45d
26.56±0.58d
443.40±36.95a
297.62±5.71b
178.47± 5.04c
142.96 ±6.00c
113.92 ±5.81c
202.27±17.72a
116.72±2.35b
93.39±3.23b
53.99± 6.23c
31.10 ±1.89c
254.99±9.62a
199.66±10.13b
164.96±10.93c
78.97± 3.06d
61.76±3.48d
161.17±18.14a
71.84±3.73b
46.34±1.46bc
38.25±4.09c
31.86±1.67c
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The results are in accordance with the findings of Khan et al.
(2000) who reported that fresh and dry weight of shoot
showed significant decrease with increase in concentration of
tannery effluent. This may be due to the presence of heavy
metals such as Cu and Cr.
3.6. Impacts of Tannery Effluent on Yield of Selected Varieties of Maize
Table 6: Effect of tannery effluents on yield parameters of maize
Maize varieties Treatments Ear/ plant Seed no./ear Seed wt/ear 100seed wt/ear
BHQPY545
T0(Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
2.66±0.20a
1.32±0.19b
1.00±00 b
0.78±0.11bc
0.45±0.22c
309.00±5.51a
272.32±2.42a
197.55±3.31b
123.32±16.39c
71.67±35.86c
84.37±4.71a
74.84±3.78ab
61.46±0.81b
45.24±5.31c
16.05±8.04d
28.60±0.15a
27.52±0.75a
25.93±0.34a
19.94±2.85ab
13.98±7.51b
Malkesa2
T0(Control)
T1 (25%)
T2 (50%)
T3 (75%)
T4 (100%)
2.66±0.20a
1.55±0.12b
1.10±0.10bc
0.78±0.11c
0.66±0.38c
303.34±5.70a
273.78±7.12a
175.56±5.84b
122.67±15.70bc
70.89±35.48c
83.58±2.17a
67.57±0.94ab
62.33±1.61b
37.67±9.79c
18.61±9.33c
27.68±0.23a
27.35±0.54a
25.83±0.45a
19.96±2.56ab
11.57±5.79b
Mean followed by different letters in the same column are significantly different at p=0.05 according to Duncan’s new multiple range test. ± Standard error, Tr =
treatments, T0 = 100% tap water (control), T1 = 25% effluent + 75% tap water, T2 = 50% effluent + 50% tap water, T3 = 75% effluent + 25% tap water, T4 = 100%
effluent
All the selected yield parameters decreased from T1 to T4
effluent concentrations in both varieties of maize. Highest
inhibitory effect was observed on all the yield parameters at
higher effluent treatment. Decreasing in ear of plant was found
with the increase of tannery treatment compared to T0
(control). A maximum of 83.08% and 75.19% reduction in ear
of plant was observed in BHQPY545 and Malkesa-2
respectively at 100% tannery effluent treatments. Seed number
was higher in control (T0) plants while gradual reduction in
seeds number was observed in all the effluent treated plants.
However, drastic change in seed number was observed in
higher concentration (T3 and T4) and effect is significant (P =
0.05). The reduction in seed number of T4 treated plants in
BHQPY545 was 76.80% while it was 76.63% in Malkesa-2.
Plants treated with T3 and T4 were extremely stunted.
Therefore, it produced fewer number of light weight seeds.
Seed weight was also higher in T0 and T1 treatments as
compared to seeds produced in T3and T4 treatments. A
maximum of 80.98% and 77.73% reduction in seed weight
was observed in BHQPY545 and Malkesa-2 respectively at
100% tannery effluent treatments. 100 Seed weight was also
gradually reduced in consecutive effluent treatment. The
magnitude of reduction in 100 seed weight per plant in T4
compared to control was 51.10%, but it was a higher value in
Malkesa-2 (58.20%). The results are in line with Turner
(2002) who reported that effluent caused severe growth
reduction in crops, particularly in cereals with marked
depression in biomass (50 - 70%). In another study, Davies
and Linsey (2001) reported grain yield reduction of 69 and
78% in two wheat cultivars due to effluent irrigation.
Similarly, Sundaramurthi (et al., 2010) reported that plant
yield is dependent on leaf growth, leaf area, and number. As
Cr affects most of the biochemical and physiological process
in plants, productivity and yield are also affected. Cr (VI) in
irrigation water decreased significantly grain weight and yield
of paddy (Oriza sativa) up to 80% under 200 mg/L of Cr
(Sundaramurthi et al., 2010).
IV. Conclusions
From the present study it can be concluded that tannery
effluent are characterized by high load of pollutants. The
effluent was dark bluish green in colour with unpleasant odour
and also characterized with high value of total dissolved
solids, electrical conductivity, BOD and acidic PH. Both
physical and chemical parameters are higher than that of the
permissible limits set for irrigation except some heavy metal.
Consequently, it shows that the effluent is not suitable for
irrigation purpose.
In both maize varieties, each tannery effluent treatments
showed inhibitory effects on vegetative and reproductive
growth. Seed germination, plumule length, Radicle length;
shoot length, root length, fresh and dry weight of shoot and
root, leaf number and all components of yield showed
inhibitory effect for each effluent treatment. The inhibitory
effect of effluent treatment is directly proportional to the
increasing percentage of effluent treatments (25%, 50%, 75%,
and 100%). The lower effluent treatment shows a bit
inhibitory effect on each vegetative and reproductive growth
parameters compared to the higher effluent treatments. This
indicates tannery effluent can be used for crop growth if
properly treated and diluted effluents.
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APPENDIX
Appendix Figure1: Germination test
Appendix Figure 2: Growth characteristics of Maize in
green house before starting treatment after 14 days of
cultivation
Appendix Figure3: During reproductive growth
ACKNOWLEDGMENT
The author thanks Ministry of Education and Haramaya
University for providing financial and institutional support.
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