Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.

1. Hygiene and RNAse-free working

2. Andreas Jefimowicz (*1984, Karlsruhe)
Training/employment industrial office clerk
Hv-Engineering
Training biotechnological laboratory assistant
Johannes-Gutenberg-School
Employment biotechnician, Celonic GmbH
Study of biotechnology,
Mannheim University of Applied Sciences
Employment Bio.Kitchen UnternehmerTUM

3. Clean room, Celonic GmbH
Production of antibodies for
immune cancer therapy
Antibodies are injected into
patients
Must be free of contaminants

7. Introduction
● handling personal protective equipment
● cleaning and disinfection
● prevent contaminations
● dispose dangerous waste

8. Some definitions…
Hygeia was the Greek goddess of
health, cleanliness and sanitation
the degree of purity correlates with health

9. Disinfection: Is the process of using a chemical reagent to significantly
reduce the concentration of a pathogenic agent (bacteria, viruses,
fungi, parasites)
Sterility: Is the state of being free of disease-causing
microorganisms
Contamination: is the present of a constituent,
impurity or some other undesirable element that spoils,
corrupts, infects, makes unfit or makes inferior a material,
physical body, natural environment, workplace etc.

10. Why do we need all this?

11. You need to protect yourself...
It is assumed that every person you are testing could
be infected with SARS-CoV-2
It is better to assume someone is infected and he or
she is not than the other way around
SARS-CoV-2 stands for “severe acute respiratory
syndrome coronavirus type 2”

12. Infection with the new coronavirus can
lead to symptoms such as cough,
runny nose, sore throat and fever,
sometimes also diarrhoea, breathing
problems and pneumonia
The main route of transmission is
droplet infection. This can occur
directly via the mucous membranes of
the respiratory tract or indirectly via
hands, which then come into contact
with the oral or nasal mucosa and the
conjunctiva.

13. You also need to protect the samples you are
working with from yourself…
Micrococcus is a airborne microorganism. It occurs ubiquitous
(everywhere)
Also when you breathe, you spread many microorganisms like
bacteria, fungi, slime molds and viruses

14. When detecting SARS-CoV-2 we have to
amplify the RNA of the virus
RNA is single stranded nucleic acids
RNAses are enzymes that lead to degradation of RNA

15. Sampling
room
Hygiene concept regarding a test centre
Sample
preparation
room
Reagent
preparation
room Amplification
room
The whole process g
high virus concentrat
high levels of possibl
contamination by am

16. Implementation of the mobile laboratory in premises such as a hospital
Ideally entry to the laboratory rooms is
via a seal / lock room
Equipment is passed through
material locks

17. What do we need?
● personal protective equipment (PPE): overall, overshoes,
goggles, ffp2 mask, tape, gloves, lab coat
● dismozon/khorsolin/bleach
● rag
● sprayer
● wash bottle
● RNAse-Erase
● water
● autoclave
● wastebags
● Ethanol 70 %
● soap
● disinfectant

18. moisten hands
Hand Hygiene - Washing Hands (40-60 sec)
apply sufficient soap rub palms in circular motion rub palms and back of hands
Palms on top of each other
and fingers interlocked for
clean finger spaces
cleaning the back of
the fingers
Hold your thumb
and clean with a
twisting motion
Rub the fingertips on the
palm of each hand
Wash off soap with
water
Carefully dry with
disposable towels
Turn off the tap using
a disposable towel
Your hands are clean now

19. Gloves are important
- as a additional barrier
- to protect your skin
- to protect you from the virus
- to protect samples from you
wear them all the time, change or disinfect them
between steps in the protocol

20. Bleaches...
● have broad spectrum bactericidal properties
making them useful for disinfecting and sterilizing
● are able to inactivate bacteria, viruses, and algae
by oxidizing them
● chlorine bleach is a strong oxidizing agent,
so you need to protect you skin and eyes

21. RNase ERASE® ...
is a precisely formulated
reagent, ready-for-use,
ribonuclease
decontaminant
We need to use it when
working with RNA
to prevent degradation

22. What are the do´s and dont´s in a lab ?

23. Do´s
● When using the lab the first time clean with Dismozon, water and ethanol
● wash and disinfect your hands before and after working, tie back long hair
● change gloves and disinfect gloves with ethanol
● disinfect patient sampling room with ethanol between every patient
● treat working surfaces with RNAse-Erase when working with RNA
● wear an overall in the patient sampling room and the sample preparation room
● wear a labcoat in reagent preparation room and amplification room
● only touch what you have to touch while working
● physical distancing of at least 1.5 meter when working together

24. Dont´s
● do not touch your face or hair while working,
● do not touch the lower part of your pipette
● avoid coughing or sneezing, and don´t talk too much
● absolutely avoid touching non-sterile items and surfaces

25. ● every morning wash hands and put on PPE, prepare Khorsolin, clean
handhelds, door knobs, surfaces, - wait 15 minutes, repeat with water
● wipe surfaces and fronts with ethanol and let them dry
● prepare your work area before you start
● in the patient treatment room use ethanol between patients to disinfect
● in the RNA Extraction area wipe surfaces/materials with RNAse-Erase
and repeat with ethanol
● same in RT-LAMP and qPCR area
● while handling patient samples and positive control wipe working area and
change gloves afterwards
● in assay run area clean with RNAse-Erase and ethanol, clean lids
● every evening clean floor with Khorsolin, wait 15 minutes, again clean
floor with water
Time Schedule

26. Sources:
Clean room: https://www.indiamart.com/proddetail/clean-room-for-pharmaceuticals-8688864812.html
Hygeia: https://www.flickr.com/photos/oar/16103293053
Bath house: https://www.pinterest.de/pin/72972456435506089/
Disinfectant agent: https://www.praxisdienst.de/Hygiene/Desinfektionsmittel/
Virus: https://pink.pharmaintelligence.informa.com/PS124172/USP-Sparks-Debate-Over-Rapid-Sterility-
Testing-For-Cell-And-Gene-Therapies
Biohazard: https://de.freepik.com/fotos-vektoren-kostenlos/biohazard-symbol
discussion round:
https://thenestline.com/staff_name/mahee-bhatt/
droplet infection: https://de.wikipedia.org/wiki/Tr%C3%B6pfcheninfektion
pneumonia: https://news.northwestern.edu/stories/2021/01/why-covid-19-pneumonia-worse-than-typical-
pneumonia/
micrococcus: https://www.istockphoto.com/de/foto/kolonien-von-micrococcus-luteus-bakterien-auf-agar-
platte-gm1148144414-309962622
airborne collector: https://www.holbach.biz/de/seite_LKS-30-amp-LKS100_15_mikrobiologie.html
RNA-virus: https://www.scinexx.de/dossierartikel/was-sind-viren/
RNA-DNA: https://www.drze.de/im-blickpunkt/abbildungen/DNA_RNA_2-de%20copyrighted.jpg/view