This study evaluated the healing of mandibular ramus bone block grafts used for alveolar ridge augmentation before implant placement through clinical, histological, and histomorphometric analysis. Bone blocks were harvested from the mandibular ramus in 15 patients and grafted to maxillary defects. After 3-9 months of healing, implants were placed and bone samples were taken. Histological analysis found signs of active remodeling but also substantial amounts of non-vital bone and generally weak neo-vascularization, suggesting that most osteocytes in the grafted bone do not survive and neo-vascularization of non-vital grafted bone is difficult. The outcomes suggest grafted bone undergoes slow remodeling into new vital bone.
This journal club presents a case of prosthetic
rehabilitation of an amputated thumb. It emphasizes that
prosthetic replacement is a better option for aesthetic and
psychological improvement, particularly in cases where the
victim is unwilling to undergo complicated surgical procedures for reconstruction of thumb or where functioning
of thumb cannot be restored even by multiple surgeries. In
the present case, a 20 years old female patient, with
missing thumb of her right hand was rehabilitated aesthetically by a non-invasive and cost effective prosthetic
procedure by using heat temperature vulcanizing silicone
material. The prosthesis (the thumb) was attached using
medical adhesives. On 3 months recall appointment, no
complications were observed. The prosthesis was in good
shape and required no further intervention. The prosthetic
thumb lacks the sensation of a normal or reconstructed
thumb, although it does not require the multiple procedures
of surgical reconstruction and the accompanying loss of
time for rehabilitation and healing
This journal club presents a case of prosthetic
rehabilitation of an amputated thumb. It emphasizes that
prosthetic replacement is a better option for aesthetic and
psychological improvement, particularly in cases where the
victim is unwilling to undergo complicated surgical procedures for reconstruction of thumb or where functioning
of thumb cannot be restored even by multiple surgeries. In
the present case, a 20 years old female patient, with
missing thumb of her right hand was rehabilitated aesthetically by a non-invasive and cost effective prosthetic
procedure by using heat temperature vulcanizing silicone
material. The prosthesis (the thumb) was attached using
medical adhesives. On 3 months recall appointment, no
complications were observed. The prosthesis was in good
shape and required no further intervention. The prosthetic
thumb lacks the sensation of a normal or reconstructed
thumb, although it does not require the multiple procedures
of surgical reconstruction and the accompanying loss of
time for rehabilitation and healing
Immediate Implant Placement And Restoration With Natural Tooth In The Maxilla...Abu-Hussein Muhamad
Anterior tooth loss and restoration in the esthetic zone is a common challenge in dentistry today. The prominent visibility of the area can be especially distressing to the patient and requires a timely and esthetically pleasing solution. Immediate single-tooth implantation followed by immediate provisionalization is becoming an increasingly desirable treatment that offers numerous benefits over conventional delayed loading. Provisionalization for immediately-placed implants using the patient’s existing tooth can enhance the final aesthetic outcome if certain steps are
followed. If the natural tooth is intact and can be used as a provisional, the emergence profile can be very similar to the preoperative condition. This article outlines a technique to use the patient’s natural tooth after extraction to provisionalize an implant.
Periodontally Accelerated Osteogenic Orthodontics with Piezoelectric Surgery...Abu-Hussein Muhamad
Piezosurgery has been applied in dentistry for many years. This paper reviews specifically the treatment applications that have been used in surgically assisted orthodontic treatment since the last decade. Periodontally Accelerated Osteogenic Orthodontics (PAOO) is a surgical technique which results in an increase in alveolar bone width, shorter treatment time, increase post-treatment stability, and decrease amount of apical root resorption. The aim of this case report is to compare the use of micro-motor and piezoelectric surgery unit during decortication in Periodontally Accelerated Osteogenic Orthodontics technique.
Key words: Piezoelectric surgery, piezosurgery, Periodontal regeneration , accelerated tooth movement
Immediate Placement and Provisionalization of Maxillary Posterior Single Impl...Abu-Hussein Muhamad
Functional immediate load is the most recent concept. This describes a load, within physiological limits, applied to implants before the osseo-integration process is completed. One of the treatment options offered to patients requiring replacement of one or more teeth is the use of prostheses supported by implants. Patients nowadays demand greater aesthetic and functional restorations; therefore, the clinician tries to reduce implant load time. All this leads to the implementation of several modifications to the conventional surgical and prosthetic protocol leading to a reduction in the load time of the implant. This is a case report of the immediate loading of posterior implant.
jornal club on Tissue Engineering for Lateral Ridge Augmentation withRecombi...Shilpa Shiv
JC on Tissue Engineering for Lateral Ridge Augmentation withRecombinant Human Bone Morphogenetic Protein 2Combination Therapy: A Case Report. IJPRD 2015.
Extraction and Immediate Implant Placement, and Provisionalization with two Y...Abu-Hussein Muhamad
Extraction and Immediate Implant Placement, and Provisionalization with two Years Follow-up: A Case Report. PDF
Abu-Hussein Muhamad, Bajali Musa, Abdulgani Azzaldeen
Bone Grafts /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
00919248678078
bone graft /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian Dental Academy: will be one of the most relevant and exciting
training center with best faculty and flexible training programs
for dental professionals who wish to advance in their dental
practice,Offers certified courses in Dental
implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic
Dentistry, Periodontics and General Dentistry.
Immediate Implant Placement And Restoration With Natural Tooth In The Maxilla...Abu-Hussein Muhamad
Anterior tooth loss and restoration in the esthetic zone is a common challenge in dentistry today. The prominent visibility of the area can be especially distressing to the patient and requires a timely and esthetically pleasing solution. Immediate single-tooth implantation followed by immediate provisionalization is becoming an increasingly desirable treatment that offers numerous benefits over conventional delayed loading. Provisionalization for immediately-placed implants using the patient’s existing tooth can enhance the final aesthetic outcome if certain steps are
followed. If the natural tooth is intact and can be used as a provisional, the emergence profile can be very similar to the preoperative condition. This article outlines a technique to use the patient’s natural tooth after extraction to provisionalize an implant.
Periodontally Accelerated Osteogenic Orthodontics with Piezoelectric Surgery...Abu-Hussein Muhamad
Piezosurgery has been applied in dentistry for many years. This paper reviews specifically the treatment applications that have been used in surgically assisted orthodontic treatment since the last decade. Periodontally Accelerated Osteogenic Orthodontics (PAOO) is a surgical technique which results in an increase in alveolar bone width, shorter treatment time, increase post-treatment stability, and decrease amount of apical root resorption. The aim of this case report is to compare the use of micro-motor and piezoelectric surgery unit during decortication in Periodontally Accelerated Osteogenic Orthodontics technique.
Key words: Piezoelectric surgery, piezosurgery, Periodontal regeneration , accelerated tooth movement
Immediate Placement and Provisionalization of Maxillary Posterior Single Impl...Abu-Hussein Muhamad
Functional immediate load is the most recent concept. This describes a load, within physiological limits, applied to implants before the osseo-integration process is completed. One of the treatment options offered to patients requiring replacement of one or more teeth is the use of prostheses supported by implants. Patients nowadays demand greater aesthetic and functional restorations; therefore, the clinician tries to reduce implant load time. All this leads to the implementation of several modifications to the conventional surgical and prosthetic protocol leading to a reduction in the load time of the implant. This is a case report of the immediate loading of posterior implant.
jornal club on Tissue Engineering for Lateral Ridge Augmentation withRecombi...Shilpa Shiv
JC on Tissue Engineering for Lateral Ridge Augmentation withRecombinant Human Bone Morphogenetic Protein 2Combination Therapy: A Case Report. IJPRD 2015.
Extraction and Immediate Implant Placement, and Provisionalization with two Y...Abu-Hussein Muhamad
Extraction and Immediate Implant Placement, and Provisionalization with two Years Follow-up: A Case Report. PDF
Abu-Hussein Muhamad, Bajali Musa, Abdulgani Azzaldeen
Bone Grafts /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy provides dental crown & Bridge,rotary endodontics,fixed orthodontics,
Dental implants courses.for details pls visit www.indiandentalacademy.com ,or call
00919248678078
bone graft /certified fixed orthodontic courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian Dental Academy: will be one of the most relevant and exciting
training center with best faculty and flexible training programs
for dental professionals who wish to advance in their dental
practice,Offers certified courses in Dental
implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic
Dentistry, Periodontics and General Dentistry.
Dr. Ahmed M. Adawy
Professor Emeritus, Dep. Oral & Maxillofacial Surg.
Former Dean, Faculty of Dental Medicine
Al-Azhar University. Numerous etiologies lie behind mandibular defects including pathologic lesions, trauma related, infectious diseases and congenital defects. At present, the methods to restore mandibular defects can be classified into four basic categories:
1.Autogenous bone grafts in the form of nonvascularized free bone transfer, or vascularized tissue transfer, either pedicled or based on microvascular anastomosis
2. Distraction osteogenesis
3. Alloplastic materials (with or without bone)
4. Tissue engineered grafts
Craniomaxillofac trauma reconstruction bone graft in cranifacial surgery/oral...Indian dental academy
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Novas diretrizes da OMS para os cuidados perinatais de mais qualidade
Histomorfometria mandibular si
1. Clinical, histological and histomorphometric evaluation of the healing of
mandibular ramus bone block grafts for alveolar ridge augmentation before
implant placement*
Alessandro ACOCELLA, Oral Surgery Specialist1
, Roberto BERTOLAI, Oral and Maxillo-Facial specialist1
,
Maurizio COLAFRANCESCHI, Professor of Human Anatomy and Pathology2
, Roberto SACCO, Oral Surgery
Programme Resident3
1
Department of Maxillo-Facial Surgery, Faculty of Medicine-University of Florence, Italy; 2
Department of Human
Anatomy and Pathology, Faculty of Medicine-University of Florence, Italy; 3
Department of Odontostomatology, Faculty
of Medicine-University of Sassari, Italy
SUMMARY. Background: Localized bone defects in the maxilla are commonly reconstructed with autologous
mono-cortical bone blocks prior to the placement of dental implants. This study presents a clinical, histological
and histomorphometric analysis on the use of mandibular ramus block grafts for ridge augmentation. Materials
and Methods: mono-cortical bone blocks from the mandibular ramus were grafted in 15 patients. The bone
grafts were left to heal for period varying from 3 to 9 months. Afterwards, 30 implants were inserted and
bone samples were removed for subsequent histological analysis. Results: All the bone grafts were successful
and resorption was minimal. There were no implant failures. At graft placement, mean lateral augmentation was
4.6 ^ 0.73 mm, which, later, at the time of implant insertion, reduced to 4 ^ 0.77 mm. Histological evaluation
indicated signs of active remodelling in all specimens. However, the grafted bone contained substantial
amounts of non-vital bone (NVB) and generally weak neo-vascularization regardless of the time of
biopsies. Conclusions: The outcome of the study suggests that the larger part of osteocytes in mono-cortical
bone do not survive grafting and neo-vascularization of non-vital grafted bone is difficult because of the slow
remodelling process into new vital. Ó 2009 European Association for Cranio-Maxillo-Facial Surgery
Keywords: bone histology, bone histomorphometry, mandibular ramus, maxillary defects, mono-cortical bone
block graft
INTRODUCTION
Bone defects in the human maxilla are common. They
are mostly determined by premature loss of teeth due
to periodontal disease or traumas. They usually cause
reduction of alveolar bone volume, which becomes, in
consequence, inadequate for standard treatments with
osseointegrated implants (Nevins and Mellonig, 1994;
Oikarinen et al., 2003).
Pathological resorption of the maxillary bone may be
the main cause of both functional and aesthetic dissatis-
faction to the patient (Collins and Nunn, 1994).
Moreover, insufficient bone height and width of the al-
veolar ridge make the maxillary anatomy particularly un-
favourable for the conventional placement of dental
implants.
In order to create favourable conditions for implant
placement, the bone must undergo augmentation. Nowa-
days, reconstruction and augmentation of severely
resorbed maxillary alveolar ridges, with the use of differ-
ent grafting materials and techniques, represent a predict-
able procedure for endoosseous implant placement.
(Buser et al., 1995; Oikarinen et al., 2003; Chiapasco
et al., 2007).
There have been experimental in vitro and in vivo
studies in all kinds of animal models in order to assess
the quality of bone defects filled with different test mate-
rials. The majority of these studies evaluated the osteoin-
ductive activities in critical size defect models using
radiography or histology (Bosch et al., 1998; Nidoli
et al., 1999).
Recently, there has been considerable interest in bone
regeneration augmented with stem cells, periosteum-de-
rived cells, or osteoinductive proteins to aid the healing
of large skeletal defects (Boo et al., 2002; Yamada
et al., 2003; Lendeckel et al., 2004; Sakata et al., 2006).
It has been widely accepted that autologous bone is the
most appropriate grafting material. (Listrom and Syming-
ton, 1988; Jensen and Sindet-Pedersen, 1991).
The harvesting sites may be intraoral or extraoral: in
the first case, bone can be harvested from retromolar,
*
The work should be attributed: Department of Maxillo-Facial Sur-
gery, Faculty of Medicine e University of Florence, Italy; Director:
Dr. Roberto Bertolai, address: Largo Palagi,1 Firenze, Italy.
222
Journal of Cranio-Maxillo-Facial Surgery (2010) 38, 222e230
Ó 2009 European Association for Cranio-Maxillo-Facial Surgery
doi:10.1016/j.jcms.2009.07.004, available online at http://www.sciencedirect.com
2. ramus or symphysis areas (Jensen and Sindet-Pedersen,
1991; Misch, 1997; Widmark et al., 1997; Hunt and
Jovanovic, 1999) while in the second case, bone can be
collected from ilium, calvarium or tibia. (Block and
Kent, 1997).
Both mandibular body and ramus area represent an
ideal donor site as they provide adequate, dense bone
with sufficient volume for implant placement and short
healing times. (Garg et al., 1998).
The advantages of the ascending ramus donor site over
the chin include minimal patient concern for altered fa-
cial contour and low levels of post-operative sensory dis-
turbances and discomfort.
Although mandibular ramus mono-cortical block
grafts for alveolar ridge augmentation have been widely
used with great clinical success, it has not been clearly
explained how they incorporate into the surrounding
bone. The general lack of histological evidence on the
healing of mandibular autogenous bone block grafts
leaves a great number of unanswered questions about
the biological process.
The vitality of osteocytes is believed to be important in
the process of bone remodelling as it seems that these
cells are involved in bone turnover.
This study’s objective was to gain insight into the vi-
tality of bone grafts in the human jaw with emphasis on
the survival of osteocytes used as sign of bone vitality.
Our study has been conducted with an integrated mix
of tests and, among them, histomorphometry which has
only been used in a few studies for the evaluation of os-
teocyte survival. (Blomqvist et al., 1998).
MATERIALS AND METHODS
Patients
Fifteen patients (7 men and 8 women) of ages ranging
from 21 to 53 years were considered for the study
(Table 1). The selected patients had severe bone defects
in the maxillary alveolar ridge, originating from peri-
odontal disease or traumas.
All 15 patients were healthy and had no general con-
traindications for bone block graft surgical treatment;
only four of them were smokers.
First of all, a clinical and radiographic investigation
was carried out and the treatment fully explained to the
patients. The treatment programme included a first oper-
ation for bone augmentation followed by a healing period
varying from 3 to 9 months (Table 1); finally, a second
surgical operation was scheduled for biopsy and implant
placement. All patients gave their informed consent to
donate the bone tissue removed during implant surgery
for histological examination.
First surgical step
The bone augmentation was carried out under local an-
aesthesia in 14 patients and under general anaesthesia
with rhino-tracheal intubation in only one case. The lat-
ter, in fact, was affected by a larger pre-maxilla defect,
which was restored with bilateral ascending ramus grafts.
Full-thickness flaps were reflected in order to allow
a satisfactory exposure of the recipient site (Figs. 1a, 2a).
The remaining crest width was measured with a pair of cal-
lipers to the nearest half-millimeter (pre-augmentation
width) (Table 2).
After administering block anaesthesia to the inferior
alveolar nerve, harvesting of bone from ascending ramus
was performed. An obliqueesagittal incision was made
distal to the third molar following the direction of the ra-
mus and a vertical releasing incision was placed distally
in the ramus area. After reflection of bucco-lingual flaps,
osteotomies were executed with a small fissure bur to
outline the dimensions of the bone block (Figs. 1b, 2b).
Careful techniques were performed to make sure penetra-
tion was only of the cortical layer and to avoid any in-
juries to the alveolar nerve. Once the osteotomy was
completed, a straight elevator was placed along the sag-
ittal cut and the lateral block of bone was green-stick
fractured off (Fig. 2c). The recipient site was prepared
by scoring or perforating through and through (with
a small, straight fissure bur) in order to create bleeding
channels. The block autograft was then fixated to recipi-
ent site with 1 or 2 lag-screws (Osteomed, Addison, TX)
(Figs. 1c, 2d). Sharp edges of the bone blocks were
rounded off (with large diamond burs) and the aug-
mented width of the bony crest was measured again
with a pair of callipers (post-augmentation width).
Gaps around the block grafts were filled with bone chips
harvested from the donor site (Fig. 2e). Once the graft
was adapted to the site, an incision through the perios-
teum at the base of the flap allowed the tissue cover
the graft without tension. The recipient and the donor
site areas were then sutured with vertical mattress sutures
(Vicryl 3-0, Ethicon, Johnson & Johnson Roma, Italy)
without covering the bone graft with a barrier membrane
(Fig. 2f).
Post-operative antibiotic (Zimox, Pfizer, Latina, Italy)
and analgesic (Brufen; Abbott SpA, Campoverde (Lt),
Italy) therapy was routinely prescribed for 7 days. Patients
were also given chlorhexidine digluconate 0.2% mouth-
wash (Curasept; Curaden Health Care Srl, Milano, Italy)
for ten days and sutures were removed after 10e12 days.
Second surgical step
After a healing period of from 3 to 9 months, before the
implant insertion, CT examination was performed in
order to plan implant surgery correctly. The flap was
re-opened with an outline similar to that of the first oper-
ation (Fig. 1d). Once the flap was elevated, the healed
crest width was measured again with a pair of callipers
(re-entry width) (Table 2). Bone block fixation screws
were removed; implant site preparation and insertion
were accomplished according to standard surgical proto-
cols. A total of 30 implants were placed (Table 1) and
primary stability was achieved during this step (maxi-
mum insertion torque was 35 Ncm).
Biopsy procedure
Biopsy was performed at the same time of implant place-
ment including the grafted bone/recipient site interface.
Clinical, histological and histomorphometric evaluation of the healing of mandibular ramus bone block grafts 223
3. Table 1 e Patient data and results of histomorphometry means
Patient Sex Age No. of implants Healing time (months) Non Vital Bone
(histological %)
1 M 28 6 4 60.7
2 M 34 2 5 34.3
3 F 32 1 4 73.2
4 M 53 2 7 52.7
5 F 37 3 9 38.4
6 F 22 2 6 42.3
7 F 52 2 6 68.2
8 M 43 1 4 73.4
9 F 49 1 3 65.2
10 M 21 2 4 62.3
11 M 38 2 5 69.8
12 F 49 1 5 80.5
13 M 32 2 4 56.2
14 F 30 1 6 42.3
15 F 42 2 6 46.8
Fig. 1 e Incisions to the recipient site (a); osteotomies of the mandibular ramus for harvesting a mono-cortical block (b); fixation of the graft to the
maxillary defect (c); re-entry at 4 months that show no clinical resorption of the graft (d); connection of the implant inserted with a zirconium abutment
and finalization of the case with a zirconium crown (eef).
224 Journal of Cranio-Maxillo-Facial Surgery
4. Fig. 2 e View of a maxillary narrow ridge after flap elevation (a); bone osteotomies on mandibular buccal shelf (b); the mono-cortical is out-fractured
(c); adaptation and fixation of the graft to the maxillary recipient site (d); gaps around the block grafts were filled with bone chips harvested from the
donor site (e); primary flap closure without tension (f).
Table 2 e Analytical description of the amount of bone augmentation obtained and grafts’ resorption
Case Residual Ridge
Width
Lateral
augmentation at
bone grafting
Post augmentation
width
Re-entry
width
Re-entry lateral
augmentation
Resorption mm Resorption %
1 3.5 mm 5 mm 8.5 mm 8 mm 4.5 mm 0.5 mm 10
2 2.5 mm 6 mm 8.5 mm 8 mm 5.5 mm 0.5 mm 8.3
3 3 mm 5 mm 8 mm 7 mm 4 mm 1 mm 20
4 3 mm 4 mm 7 mm 6.5 mm 3.5 mm 0.5 mm 12.5
5 3.5 mm 4 mm 7.5 mm 7.5 mm 4 mm 0 mm 0
6 4 mm 5 mm 9 mm 7.5 mm 3.5 mm 1.5 mm 30
7 3 mm 5 mm 8 mm 8 mm 5 mm 0 mm 0
8 3.5 mm 4 mm 7.5 mm 7 mm 3.5 mm 0.5 mm 12.5
9 3 mm 5 mm 8 mm 7.5 mm 4.5 mm 0.5 mm 10
10 2.5 mm 5 mm 7.5 mm 7 mm 4.5 mm 0.5 mm 10
11 2 mm 4 mm 6 mm 5.5 mm 3.5 mm 0.5 mm 12.5
12 3 mm 4 mm 7 mm 6 mm 3 mm 1 mm 25
13 4 mm 5 mm 9 mm 8 mm 4 mm 1 mm 20
14 3.5 mm 3 mm 6.5 mm 6 mm 2.5 mm 0.5 mm 16.6
15 3 mm 5 mm 8 mm 7.5 mm 4.5 mm 0.5 mm 10
Clinical, histological and histomorphometric evaluation of the healing of mandibular ramus bone block grafts 225
5. A bone specimen measuring approximately 2.5 mm di-
ameter and 8e10 mm long was removed with a hollow
trephine burr of 3 mm outer diameter, accompanied by
a copious irrigation. The specimen was carefully re-
moved from the trephine burr and the part of the biopsy
corresponding to the buccal graft’s cortical plate was la-
belled by black Indian ink, for better orientation during
histology examination.
Histology and histomorphometry
All specimens were immediately fixed in 4% formalde-
hyde solution in 0.1 M phosphate buffer (pH 7.3) at
4
C for 24 h. They were then rinsed three times with
0.1 M phosphate buffer and, finally, stored at 70% etha-
nol at 4
C, until ready to be embedded. All the biopsies
were cold embedded in methylmethacrylate with 20%
N-plastoid (resin solution). Nondecalcified, 5-mm thick
sections were made along the axis of the biopsy using
a Jung K microtome. All specimens were, then, stained
with haematoxylin and eosin. A Leica DM RA micro-
scope connected to a computer using an electronic stage
table and a Leica DC 200 digital camera were used for
histomorphometrical measurements. Leica QWinÓ
soft-
ware (Leica Microsystems Image Solutions, Rijswijk,
the Netherlands) was used to process and measure the
digitised images. This software allows a selection of parts
of an image by setting a threshold to the colour of the
pixels, which can then, be measured. All measurements
were carried out at Â200 magnification in order to allow
clear distinction between empty and full osteocyte lacu-
nae. The sections stained with haematoxylin and eosin
were used for the measurements of total bone volume
and NVB volume. The mineralized bone tissue containing
areas of empty osteocyte lacunae was defined as NVB.
NVB was expressed as a percentage of the total bone
volume and its measurement was made semi-automati-
cally by outlining the area of empty osteocytes lacunae.
Statistical analysis
All data were analysed using SPSS for Windows 12.0. A
simple regression model was applied to the data in order
to examine the correlation between healing time and
NVB (Model: NVBi ¼ b0 + b1 moni + 3). The data were
presented as means and standard deviations. Significance
was accepted at the p0.05 level.
RESULTS
Clinical
All grafts integrated successfully, guaranteeing sufficient
bone volume area for implant installation. No significant
resorption around the head of the fixation screws was evi-
dent. Only one patient exhibited a small graft exposure but
the soft tissue dehiscence resolved spontaneously showing
no further complications graft infections. Another patient,
who underwent a large bilateral graft from the mandibular
body, experienced a transient hypoesthesia of the lower
lip, which recovered completely within three weeks.
Table 2 analyses the analytical data regarding the
grafting together with rise of bone volume at the time
of grafting and implant placement. The mean bone
resorption occurring during healing time was calculated
according to the measurements of lateral augmentation
at bone grafting and after 3e9 months, at implant place-
ment. Considering all the sites together, mean lateral
augmentation, at grafting, was 4.6 ^ 0.73 mm which re-
duced to a mean of 4 ^ 0.77 mm at implant insertion;
this is equivalent to a mean reduction in the lateral aug-
mentation of 13.1 ^ 8.9% (min 10%, max 30%) during
healing (Table 3).
The number of fixtures placed and the timing of inser-
tion for each patient are indicated in Table 1. After six
months all 30 implants placed were loaded and all fix-
tures were successful, according to Albrektsson criteria
(Albrektsson et al., 1986) (Fig. 1eef). In the subsequent
follow-up (average 12-month follow-up), no major com-
plications were recorded at donor or recipient sites. Soft
tissue healing was uneventful, while pain and swelling
were comparable to conventional dento-alveolar proce-
dures. Wound dehiscence with graft infection and defin-
itive neurosensory deficits at the donor site wase not
detected.
HISTOLOGICAL AND HISTOMORPHOMETRIC
FINDINGS
Histologically, all specimens showed signs of active re-
modelling and all tissues were free of inflammatory cells.
Contemporarily, all sections contained both vital and
NVB tissue, compact osteonic and trabecular bone. The
representation of vital and NVB bone varied consider-
ably according to each individual. Bone classed as non-
vital (with fields of empty osteocyte lacunae) was pre-
dominantly lamellar in type (Fig. 3), while the vital
bone was composed of both lamellar and woven bone.
The vital and NVB were in contact with each other
and, sometimes, the latter was completely surrounded
by vital bone the former first. The margin between the vi-
tal and the NVB coincided with an abrupt change in the
orientation of the lamellae (Fig. 3).
As vital bone was in tight contact with the NVB (os-
teoconductor), the first replaced the second thanks to
creeping substitution process (Fig. 4). Osteoclasts were
rarely detected. Vital bone contained osteocytes in the in-
ner core of an osteon, surrounded by NVB, suggesting
that this was recolonized by blood vessels (BV) and os-
teogenic cells via the Haversian canal (Fig. 3).
Neovasculature was poorly represented in all speci-
mens. At the interface between grafted bone and the re-
cipient site, some specimens showed varying amounts
of fibrous tissue (FT) mixed with new bone formation
(Fig. 4). With the histological analysis alone, very little
qualitative differences could be found among biopsies
at different healing times. The volume of vital and
NVB of all patients and the time of healing is presented
in Table 1. The relative NVB volume (NVB as % of total
bone volume) varied from 80.5% (in patient no. 12) to
34.3% (in patient no. 2) with an overall average of
57.75% of total tissue volume.
226 Journal of Cranio-Maxillo-Facial Surgery
6. Statistical analysis
The relationship between variables ‘‘NVB’’ (response
variable) and ‘‘Months’’ (explanatory variable, length
of time) looked linear (Fig. 5). A straight line was extrap-
olated from the data by the least squares method. The
estimated slope ^b 1 was negative, indicating that the
estimated decrease in NVB per month is around 5.2%.
The associated R-squared was around 31%, and its
p-value (as well, of course, as the p-value of the wald
test on the slope coefficient) was moderately significant,
showing that the linear relation is good enough. While
most of the data points were clustered towards the left
side of the plot, there is just one an observation point,
which lies far away from the main cluster of the data
(Fig. 5). As this point probably has a major impact on
the regression line, another model omitting this observa-
tion was tried. These data suggest that the amount of
NVB decreased slowly as time of healing increased;
grafted bone resulted completely revascularized and
remodelled after 16.2 months (Fig. 5).
DISCUSSION
Preprosthetic reconstructive surgery of the resorbed max-
illa has to create sufficient, good quality bone for implant
placement. Several procedures have been proposed to
achieve alveolar ridge augmentation in partially edentu-
lous patients. (Buser et al., 1995; de Wijs, 1997).
Bone block graft is the preferred method for many types
of augmentation procedures, since it secures both a source
of osteogenic cells and a rigid structure for mechanical
support. In addition, bone block graft conserves its volume
better than particulate grafting (Lew et al., 1994).
Autologus bone block grafts have a greater flexibility, as
they can be used in all clinical situations (also in cases of
complex), while on the other hand, they show a greater
potential for post-operative morbidity (risk of graft infec-
tion and neurologic sequelae related to bone harvesting
in intra-oral site). In fact, harvesting of grafts from the
mandibular region may cause neurological complications,
due to the risk of damage to the inferior alveolar nerve.
Recently, a sensory deficit in lower lip and mental areas
Table 3 e Mean and standard deviation in mm of lateral bone augmentation measured at the time of bone grafting and surgical re-entry for implant
placement. Mean percentage reduction in lateral augmentation after grafts healing
Lateral augmentation at bone grafting Re-entry lateral augmentation Resorption %
4.6 ^ 0.73 4 ^ 0.77 13.1 ^ 8.19
Fig. 3 e Histology of the external cortical layer of the ascending ramus graft after 4 (a), 5(b), 6(c; d) months. The pictures show the typical compact
osteonic structure of the mandibular ramus but with predominantly empty osteocyte lacunae (NVB) at different healing times. Signs of active
remodelling and new growing vessels are not evident; at a higher magnification vital bone containing osteocytes in the inner core of osteons, surrounded
lamellar non-vital bone suggesting that non-vital bone was recolonized by BV and osteogenic cells via the Haversian canals (d). o ¼ osteocyte,
bv ¼ blood vessel, NVB ¼ non-vital bone; original magnification Â100 (a,b) and Â200 (c,d); specimens stained with haematoxylin and eosin.
Clinical, histological and histomorphometric evaluation of the healing of mandibular ramus bone block grafts 227
7. Fig. 4 e Grafted bone/recipient site interface after 4 (A) and 6 months (B). (a1) After 4 months a substantial amount of FT, little new vital bone; (a2) at
a higher magnification new vital (VB) bone surrounded by NVB with empty osteocyte lacunae, way of resorption (creeping substitution). (b1) After 6
months: active bone remodelling with presence of larger amount of vital bone; FT is still present and NVB has not been completely resorbed. (b2) At
a higher magnification, a large amount of new vital bone containing osteocytes; original magnification Â100 (a1,b1) and Â200 (a2,b2); specimens
stained with haematoxylin and eosin.
Fig. 5 e Non-vital bone as percentage of total bone volume relative to time.
228 Journal of Cranio-Maxillo-Facial Surgery
8. of 8.3% in mandibular ramus harvesting was reported,
compared with 16% for the chin as the donor site.
Our clinical findings showed that 9 months post-graft-
ing is a sufficient period of time for the grafted bone to
integrate successfully with the original maxillary bone
and reach the proper stability for an ideal implant
placement. Moreover, in accordance with literature, the
resorption rates reported confirmed the excellent volu-
metric stability of the bone block grafts harvested from
ascending ramus (Braun and Sotereanos, 1984; Misch
et al., 1992; Raghoebar et al., 1996; Cordaro et al.,
2002; Schwartz-Arad and Levin, 2005).
Histologically, this work shows that the grafted bone
contained extended parts of NVB (with empty osteocyte
lacunae) at the time of implant placement. In fact, osteo-
cytes require a 0.1-mm proximity to nutrient vessels
(Ham, 1952) to survive and interruption of the blood sup-
ply results in avascular necrosis (Cruess, 1986). In this
sense, our findings correspond with that of Ellegaard
et al., 1975 who state that with disruption of BVs,
many osteocytes are completely entombed by mineral-
ised bone and do not survive the relocation.
Therefore, the presence of deficient neovasculature at
different healing times suggests that the dense structure
of cortical bone represents a physical obstacle the new
vessel growth both from the soft and hard tissues.
From a clinical point of view, it seems important to
wait for NVB to be replaced by vital bone prior to im-
plant placement, since vital bone has better mechanical
characteristics (Goldberg and Stevenson, 1987). In this
respect, the rate by which NVB is resorbed by osteoclast
activity followed by new bone formation is an important
factor for the osseointegration of implants. There is in-
creasing evidence that osteocytes are mechanosensitive
(Klein-Nulend et al., 1995) and, for this, they play a cru-
cial role in adaptive bone remodelling. Osteocytes may
be involved in the recruitment of osteoclasts or in the
modulation of their activity by secreting signalling factors,
such as nitrogen oxide, prostaglandins (Klein-Nulend
et al., 1995), osteoprotegerin, macrophage colony-stimu-
lating factor (M-CSF) and receptor activator of nuclear
factor kappa B ligand (RANKL) (Zhao et al., 2002) or fac-
tors related to the apoptosis pathway (Bronckers et al.,
1996) induced by ischaemia (Kikuyama et al., 2002).
These factors communicate with the bone surface and con-
trol the activity of bone surface cells such as osteoblasts,
osteoclasts and bone lining cells. (Smit and Burger,
2002; Zhao et al., 2002; Zerbo et al., 2003).
As the sample of patients considered by this work is
not relevant, this study does not contribute extensively
to set new guidelines for the clinical management of
the correct timing of implant placement after grafting.
However, the slow revascularization and remodelling
processes of mandibular bone blocks suggest waiting at
least 4 months after grafts healing before implant inser-
tion. (Aalam and Nowzari, 2007; Felice et al., 2009).
CONCLUSIONS
From a clinical point of view, as supported by literature,
this procedure appears to be simple, safe and effective for
the treatment of localized alveolar ridge defects in par-
tially edentulous patients. Nonetheless, more studies are
needed to explain the internal micro architectural
changes occurring during the incorporation and remodel-
ling processes in order to draw more definitive conclu-
sions about the correct timing of implant insertion and
loading.
ACKNOWLEDGMENTS
The authors would like to acknowledge Paolo Nardi MD, DDS,
Carlo Catelani MD, DDS, Pasquale Paglianiti, DDS for their
clinical contribution and overall assistance and Miss Ilaria Pas-
quinelli for English review.
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a histological and histomorphometric study. Clin Oral Impl Res 14:
759e766, 2003
Zhao S, Zhang YK, Harris S, Ahuja SS, Bonewald LF: MLO-Y4
osteocyte-like cells support osteoclast formation and activation.
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Dr. Roberto SACCO
Via Alcide De Gasperi
113 Prato (59100)
Italy
Tel: +39 349 5858220
E-mail: saccoroberto@yahoo.it
Paper received 7 January 2009
Accepted 3 July 2009
230 Journal of Cranio-Maxillo-Facial Surgery