Descripton of HPLC, it's components, mechanism and uses.

1. PRESENTED
BY-
SAGARDEEP SINHA
M.Sc. STUDENT
UTTAR BANGA KRISHI VISWAVIDYALAYA

2. WHAT IS CHROMATOGRAPHY?
 Chromatography is a technique to separate
mixtures of substances into their components on the
basis of their molecular structure and molecular
composition. This involves a stationary phase (a
solid, or a liquid supported on a solid) and a mobile
phase (a liquid or a gas). Chromatographic
separations can be carried out using a variety of
stationary phases, including immobilized silica on
glass plates (thin-layer chromatography), volatile
gases (gas chromatography), paper (paper
chromatography) and liquids (liquid
chromatography).

3. HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY
 High-performance liquid chromatography (HPLC; formerly
referred to as high-pressure liquid chromatography) is a
technique in analytical chemistry used to separate, identify, and
quantify each component in a mixture. It relies on pumps to
pass a pressurized liquid solvent containing the sample mixture
through a column filled with a solid adsorbent material. Each
component in the sample interacts slightly differently with the
adsorbent material, causing different flow rates for the different
components and leading to the separation of the components as
they flow out of the column.
 HPLC is distinguished from traditional ("low pressure") liquid
chromatography because operational pressures are significantly
higher (50–350 bar), while ordinary liquid chromatography
typically relies on the force of gravity to pass the mobile phase
through the column.

4. COMPONENTS OF HPLC
 Mobile phase reservoirs
 Injector system
 Column
 Detector
 Pump
 Waste
 Recorder
The other three systems include
The four main systems include

7. Applications of HPLC:
• Pharmaceutical Applications
• Environmental Applications
• Applications in Forensics
• Food and Flavor
• Applications in Clinical Tests