Chromatography is a method used to separate mixtures into individual components. It involves passing a mixture through two phases - a stationary phase and a mobile phase. The different components in the mixture interact with the phases at different rates, allowing separation. Chromatography is used in various fields like chemistry, environmental science, and forensics to analyze samples and isolate specific components. Common techniques include column chromatography, thin layer chromatography, and size exclusion chromatography.

1. By
Lubna Ahmed
Shantel Hernandez
Keisha Napper
Dayimwende Ouedraogo
Christine Pennington
Sunny Schemery

2.  Chromatography is a method used for the
separation of a mixture of compounds into its
individual components
 The purpose is to detect or quantitate a particular
component to be assayed in a pure form.
 Chromatography has 2 phase:
 Stationary Phase: the material in the column for which the
components to be separated having varying affinities.
 Mobile Phase: comprised of solvents into which the
sample is injected. Referred to as “carrier fluid” due to
the solvent and the sample flow through the column
together.

3.  The stationary phase stays fixed in place while the
mobile phase moves the components of the
mixture through medium or column being used.
 Stationary phase acts as a constraint, slowing
down the movement of some components in the
mobile phase.
 Different factors of a component affect the
affinities for the stationary phase, which makes
components move faster or slower facilitating the
separation of the components in the mixture.

4.  The Column: used in most
application of chromatography.
It is were the actual separation
takes place.
 Usually made of glass or metal
tube of with enough strength to
withstand the pressure that may
be applied across it.
 The column contains the
stationary phase
 The mobile phase runs through
the column and is absorbed
onto the stationary phase.
 The column is either a packed
bed or open tubular

5.  Feed injection into the mobile phase flows through the
system by the action of a pump.
 Separation in the Column- sample flows through and the
different components will absorb to the stationary phase to
different degrees. A strong attraction will move slower than
components with a weak attraction. This is how the
separation occurs.
 Elution from Column- when sample is flushed/displaced
from stationary phase the different components will elute at
different times. Weak attraction will come out first while
strongly attracted components will be last.
 Detection- the different components are collected as they
come out of the column. A detector analyzes the stream by
measuring a property related to concentration and
characteristics of chemical composition.

7.  Used to separate
different particles in a
solution for analyzing.
 Used in chemistry as a
method of isolating
certain particles for
testing
 Size exclusion/ Gel
Chromatography
 Used in environmental
science, food, cosmetics,
forensic etc.

8.  Used to identify drugs
present in blood or
Thin Layer
Chromatography
 urine.
 Used with:
 Athletes
 Job applicants
 People on probation

9.  Click/Copy link below
 http://www.separations.us.tosohbioscience.com/Servi
ceSupport/TechSupport/ResourceCenter/Principlesof
Chromatography/SizeExclusion

10.  Carrier, R. (n.d.). Chromatography. Retrieved from http://www.rpi.edu/dept/chem-eng/Biotech-
Environ/CHROMO/chromintro.html

Chromatography [Web Graphic]. Retrieved from http://www.m2c3.com/chemistry/VLI/M4_Topic2/la_16_07.jpg
Chromatography. (2013). Retrieved from http://science.howstuffworks.com/chromatography-info.htm

Column Chromatography [Web Graphic]. Retrieved from wdict.net/word/Column chromatography

High Performance Liquid Chromatography (HPLC). (2008). In S. Bell, Facts on File Science Library. Encyclopedia of
Forensic Science, Rev. ed. (pp. 188-189). New York: Facts on File. Retrieved from
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
Size Exclusion Chromatography [Web Graphic]. Retrieved from
http://bricker.tcnj.edu/tech/BIOL311chromatography.htm

Thin Layer Chromatography [Web Graphic]. Retrieved from http://withfriendship.com/user/svaruna/thin-layer-
chromatography.php

Testing athletes for drug uses. (2013). Retrieved from http://entertainment.howstuffworks.com/athletic-drug-test8.htm
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(2013). World Book, Inc. Retrieved from http://www.worldbookonline.com/pl/infofinder/article?id=ar113900
 Thin Layer Chromatography [Web Graphic]. Retrieved from http://withfriendship.com/user/svaruna/thin-layer-
chromatography.php