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The goals of the human genome project included sequencing the entire human genome, identifying the complete set of human genes, creating databases to store the genomic data, and addressing ethical issues. The project used two main methods: expressed sequence tags to identify genes and RNAs, and sequence annotation to sequence the entire genome and later assign functional tags. The process involved isolating DNA from cells, fragmenting it, amplifying fragments using vectors, sequencing the fragments, assembling sequences based on overlaps, storing the data in databases, and genome mapping using microsatellites.
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- 1. Goals of the human genome project Goals of the human genome project include: •Optimization of the data analysis. •Sequencing the entire genome. •Identification of the complete human genome. •Creating genome sequence databases to store the data. •Taking care of the legal, ethical and social issues that the project may pose.
- 2. Methods of the human genome project In this project, two different and significant methods are typically used. 1.Expressed sequence tags wherein the genes were differentiated into the ones forming a part of the genome and the others which expressed RNAs. 2.Sequence Annotation wherein the entire genome was first sequenced and the functional tags were assigned later.
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