A group of senior veterinary officers visited farms in Gulmi and Arghakhanchi districts of Nepal to investigate complaints from farmers. They collected 20 total samples from animals, including blood, serum, feces, and nasal swabs. Laboratory testing was performed on the samples at the Regional Veterinary Laboratory in Pokhara. Most test results were normal, except blood glucose was very low, calcium was slightly low, and magnesium was higher than normal ranges. Only one fecal sample tested positive for parasites. The report recommends restoring normal calcium and glucose levels and vaccinating for PPR virus.

1. Prepared by:
Dr. Krishna Prasad Acharya
Regional Veterinary Laboratory, Pokhara, Nepal
Presenter:
Dr. Kedar Raj Pande
Senior Veterinary Officer,
Regional Veterinary Laboratory, Pokhara

2.  A group of senior vet officer visited the site based on the call and
complain by the farmers. The group of senior vets suspected and
recommended the testing for herd health. Then, staffs from RVL,
Pokhara headed to Gulmi and Arghakhanchi and took the samples
following standard protocol.
 A total of 20 samples (Blood, serum, Fecal and nasal swabs) were
taken for the examination.
 Temperature from each animal was also recorded; the temperature
was within normal range.

3. A total of 20 fecal samples were collected per rectum following the standard
protocol and examined at Regional Veterinary Laboratory, Pokhara, Nepal.
Location ID EPG OPG
Gulmi FL 19 Neg 800
Gulmi FL 03 Neg 300
Gulmi 102 500 15000
Gulmi DPK 5113 2800 14000
Gulmi DPK 5143 200 3000
Gulmi FL 22 800 20500
Gulmi 23 200 Neg
Gulmi FL 98 700 Neg
Gulmi FL 46 200 Neg
Gulmi FL 50 300 Neg
712.50±875.76
8993.33±8627.32

4. Location ID EPG OPG
Arghakhanchi FL 2 Neg 2300
Arghakhanchi FL 36 Neg 1800
Arghakhanchi DPK 5110 200 16000
Arghakhanchi FL 62 100 1100
Arghakhanchi FL 101 300 9900
Arghakhanchi DPK 5328 Neg 20000
Arghakhanchi 26 Neg 2100
Arghakhanchi FL 33 Neg 7000
Arghakhanchi FL 54 Neg Neg
Arghakhanchi F 100 Neg 2000
Mean 200.00±100.00 6911.10±6993.64

5.  Out of 20 samples tested, only one sample (
FL-54 from Arghakhanchi) was found positive.

6. Code
Calcium Phosphorus Magnesium Glucose Total Protein
FL 19 7.86408 7.327272727 2.140350877 2.906977 7.652968
FL 03 8.55987 7.472727273 8.070175439 7.267442 7.214612
102 9.23948 8.181818182 5.578947368 6.395349 8.146119
DPK 5113 8.51133 8.709090909 3.403508772 18.31395 10.41096
DPK 5143 8.57605 10.05454545 3.754385965 7.267442 7.269406
FL 22 9.36893 8.127272727 3.122807018 22.67442 6.630137
23 9.35275 15.94545455 2.912280702 1.453488 7.872146
FL 98 8.98058 7.381818182 2.701754386 13.0814 7.872146
FL 46 10.1294 7.109090909 3.122807018 8.72093 8.365297
FL 50 6.84466 8.672727273 11.75438596 9.011628 7.780822
Mean
8.74±0.91 8.90±2.63 4.66±3.04 9.71±6.60 7.92±1.00
Reference value
8.9-10.6 3.2-9.8 2.1-2.9 60-100 6.4-7.8

7. Code
Calcium Phosphorus Magnesium Glucose Total Protein
FL 2 8.38188 13.38181818 5.859649123 12.7907 8.054795
FL 36 7.57282 9.145454545 7.087719298 9.302326 7.634703
DPK 5110 5.61489 9.727272727 13.54385965 4.651163 7.105023
FL 62 8.72168 5.563636364 4.280701754 7.55814 7.525114
FL 101 7.83172 10.32727273 5.192982456 3.488372 7.050228
DPK 5328 8.41424 7.581818182 3.964912281 7.55814 7.269406
26 9.96764 8.254545455 4.842105263 12.2093 8.694064
FL 33 9.4822 8.618181818 4.49122807 12.7907 8.547945
FL 54 8.3657 6.163636364 3.368421053 18.31395 7.652968
F 100 8.22006 10.43636364 5.719298246 5.523256 6.757991
Mean 8.26±1.17 8.92±2.26 5.83±2.91 9.41±4.59 7.62±0.63
Reference value 8.9-10.6 3.2-9.8 2.1-2.9 60-100 6.4-7.8

8. Code
Hb PCV Neut % Lymp % Eosin % Mono % Baso %
FL 19 9 27 30 52 16 2 0
FL 03 6 19 30 54 14 2 0
102 10 30 24 62 14 0 0
DPK 5113 6 18 34 62 4 0 0
DPK 5143 10 30 30 56 12 2 0
FL 22 9.2 30 31 60 6 3 0
23 9 29 22 50 18 10 0
FL 98 8.6 27 26 60 14 0 0
FL 46 10 26 28 52 14 6 0
FL 50 11 30 30 53 12 7 0
Mean
8.88±1.67 26.60±4.52 28.50±3.57 56.10±4.53 12.40±4.29 3.20±3.39 0
Reference
value
10.02±0.61 31.00±2.54 28.30±4.06 55.60±2.50 12.20±3.08 4.00±1.41 0-1

9. Code
Hb PCV Neut % Lymp % Eosin % Mono % Baso %
FL 2 10 34 25 56 14 5 0
FL 36 10 30 26 55 13 6 0
DPK 5110 11 34 30 52 12 6 0
FL 62 9 27 26 56 14 4 0
FL 101 10.2 32 26 54 16 4 0
DPK 5328 10.4 33 30 58 11 2 0
26 10.2 31 30 54 14 2 0
FL 33 9 27 24 58 14 4 0
FL 54 10 30 38 53 6 3 0
F 100 10.4 32 28 60 8 4 0
Mean
10.02±0.61 31.00±2.54 28.30±4.06 55.60±2.50 12.20±3.08 4.00±1.41 0
Reference
value 8.0-12.0 22.00-38.00 30.00-48.00 50.00-70.00 1.00-8.00 0.00-4.00 0-1
*Feldman et al. (2002), from Schalm’s Veterinary Hematology.
Philadelphia. Baltimore.

10.  All the blood parameters were normal except
blood glucose, calcium and magnesium
 Blood sugar level was very low while blood
calcium was slightly low
 Magnesium was higher that could be due to the
feeding of young shoots and young foliage.
 All the tested samples were negative for blood
protozoa

11.  Immediate measures to restore the normal blood
calcium and glucose level, and PPR
vaccination is suggested.

12. Thank you!!