Status of disease in the state number 4 on the basis of epidemiological study and laboratory cases

1. Prepared by:
Dr. Krishna Prasad Acharya
Veterinary Officer
Regional Veterinary Laboratory, Pokhara
Presented by
Dr. Kedar Raj Pande
Senior Veterinary Officer
Regional Veterinary Laboratory, Pokhara

2. 1810 4 2
291828
6 17
425
5
154
6
31
286
41
0
50
100
150
200
250
300
350
400
450
No. of Outbreak
No. of Outbreak

3. Disease outbreak situation in
western region-2017
0
50
100
150
200
250
300
350
400
450
FMD
PPR
ClassicalswineFever
SheepandGoatPox
ND
Rabies
Babesiosis
HS
FowlCholera
FowlPox
FowlTyphoid
IBD
Mycoplasmosis
Pullorum
Coccidiosis
Enterotoxemia
FY 2016
FY 2017

4. -
Poultry : 1966 negative for Coccidia
793 positives for Coccidia
(28.74%)
– 2 sample was found positives for the
Demodex canis


5. Total Sample Tested: 300
Positives: 238 Negative : 62
: Kaski-Lwanghelal VDC
(Ward 9), Shiding (Positive 90%), Ghana
Pokhara-Lamjung (Positive 80%), Mustang
District Jomsom,Thini (Positive 68%)
Strongyloides, Strongylus and Monezia sps

6. Clinical Mastitis in Cattle and
Buffaloes at RVL, Pokhara
In Clinical Mastitis cases clients,
technicians brings samples to the RVL
for the cultural examination.
 SLST test Positive milk samples = 414 (69.93%)
 SLST test Negative Milk Samples = 182 (30.74%
Total Sample Received : 592
Animals

7. Samples Received From
Districts
S.
N
District Animal
Species
Sample
Received
Date Test methods Remarks
1 Animal
Quarantine
Office
Rupandehi
Goat 63 Ashoj ELISA,
Brucella
All
Negative
2 Tanahu Cattle 28 Shrawan Serum
Biochemistry
Normal
3 Kapilvastu Goat 28 Kartik ELISA 24+ve
4 Kapilvastu Goat 9 sharwan Penside test 6+ve
5 Myagdi Buffalo 1 Kartik Giemsa
Staining
NAD

8. Samples Received From
Districts
S.
N
District Animal
Species
Sample
Received
Date Test
methods
Remark
s
6 Kaski Goat 6 Chaitra Penside
Test
All
Negative
7 Palpa Goat 3 Magha Penside
Test
1 +ve
8 Tanahun Goat 3 Baishkha Penside
Test
All
Positive
9 Nawalparasi Goat 9 Ashad Penside
Test
All
Positive
10 Mustang Goat 20 Ashad Penside
Test
6 +ve

9. Antibiogram of Mastitis
Milk Samples
99
23
37
17 5
87
0
5
10
15
20
25
30
35
40
45
50
146 Plastic bottled samples were not cultured

10. Anti-biogram of milk
sample tested
0
25
12
1
60
6
2
13
1 2
0 00
24
21
8
10
49
24
10
0 0 1 0
49
7 6
24
1
16
24
1 0 0 0 1
0
10
20
30
40
50
60
70
HS
MS
R

11. Post Mortem and Lab Diagnosed
Poultry Diseases
Diseases Total Percentage
Colibacillosis 714
19.41
CRD 276 7.50
Coccidiosis 367 9.98
Infectious Bursal
Disease
420 11.42
Salmonellosis 296 8.05
Mycotoxicosis 235 6.39
Immunsupression 112 3.04
New Castle Disease 144 3.91
Omphalitis 213 5.79
Ascites 486 13.21
Avian Influenza 31 0.84
Others 383 10.41
Total cases (n) = 3677

12. Virological Examinations
Species
Number
of
Sample
Tested
For
Test
Method
Applied
Results
Goats 54 PPR
Pen side
test
24 +ve
Pig/Goat/
Dog
33 Rabies Rapid test
1+ve
Goat
Poultry 260 IBD
IBDV rapid
test
134+ve
Poultry 32
Newcastle
d/s
NDV rapid
test
7 +ve
Poultry 327
Avian
Influenza
AIV rapid
test
31 +ve
(18 H9)
Pig 42 CSF AB ELISA 41 +ve

13. Serological Examinations
Species
Number
of Serum
Tested
For
Test
Method
Applied
Results
Cattle 55 Brucellosis RBPT
All
Negative
Buffalo 32 Brucellosis RBPT
All
Negative
Dog 6 Brucellosis RBPT
All
Negative
Goat 115 Brucellosis RBPT
All
Negative
Poultry 80
Salmonellos
is
PAT 42 +ve
Poultry 80
Mycoplasm
osis
PAT 21 +ve

14. PPR ELISA Report of Serum
samples from Goat and Sheep
(074/075)
S.N District Total
Sample
Positive Negative Doubtful
1 Rupendehi 81 75 3 3
2 Kapilbastu 80 57 18 5
3 Nawalparasi 12 10 2 0
4 Palpa 81 76 5 0
5 Parvat 22 19 3 0
6 Lamjung 81 48 21 12
7 Tanahun 46 30 4 12
8 Baglung 43 32 11 0
9 Gorkha 46 34 7 5
10 Myagdi 49 44 3 2
Total 541 425 77 39

15. Serum Biochemistry ( n=28;
Tanahu)
S.N. Calcium Phosphorus
Magnesiu
m Total Protein Glucose
1 1.283573 6.554622 1.4913 7.816513761 30.6122
2 7.969231 4.705882 0.9562 7.908256881 24.4897
3 6.553846 7.247899 1.5953 6.623853211 2.91545
4 6.307692 4.957983 1.5722 7.52293578 24.8554
5 7.446154 7.331933 1.526 7.779816514 30.6122
6 11.6 5.357143 1.7687 9.651376147 14.2857
7 8.215385 6.911765 2.3583 9.834862385 45.6647
8 8.553846 10.94538 1.919 11.1559633 41.6184
9 10.15385 5.420168 1.9421 9.871559633 22.1574
10 9.969231 8.004202 1.8497 9.266055046 18.0758
11 9.138462 6.281513 1.6994 9.211009174 40.8163
12 8.123077 10.2521 3.3872 12.34862385 13.1195
13 10.49231 8.193277 1.7572 9.431192661 8.45481
14 8.769231 5.882353 1.8034 7.926605505 8.45481
15 8.676923 8.55042 1.5953 7.963302752 30.3206
16 9.107692 7.710084 1.6531 10.03669725 25.3644
17 7.046154 4.747899 1.7687 7.394495413 19.2419
19 8.953846 4.852941 1.526 7.28440367 23.6151
20 9.6 5.39916 2.289 9.963302752 32.9446
21 8.307692 8.109244 2.3583 8.330275229 22.4489
22 10.64615 4.348739 1.2369 8.348623853 6.70553
23 7.753846 12.2479 2.0693 7.908256881 51.0204
24 8.307692 4.88 1.3872 13.35779817 13.7026
26 7.169231 5.798319 1.1907 7.724770642 20.6997
27 7.507692 6.134454 1.4219 8.752293578 30.3206
28 7.384615 6.386555 1.6184 8.568807339 18.9504
29 9.2 6.869748 1.1791 8.275229358 17.7842
30 8.984615 6.233894 1.7456 8.623853211 30.6122
SE 1.869029 1.951369 0.468861 1.52693373 11.817846
Mean 8.329358 6.796985 1.738054 8.888597641 23.923696

16. RVL as a Vaccine Bank
PPR Vaccine
No. of vaccines Stock till Date :
100000
No. distributed till Date 075/3/32:
22700
Remaining Stock: 77300
Anti Rabies vaccine
No. of vaccines received till date:
3000
No. distributed till Date 075/3/32:
2920
Remaining Stock: 80

17. Facilities needed for further
improvement of Animal
Disease Diagnosis !!!
• Availability of adequate funds to purchase
reagents for microbiological testing
• Availability of adequate fund to purchase
chemical reagents, gel and PCR
primers to run PCR
• Provision of Air conditioning systems to
run lab smoothly
• Regular refresher training to personnel to
upgrade their capabilities especially on
molecular testing and microbiological
culture and test.

18. Thank you !!!!!