Prepared by:
Dr. Krishna Prasad Acharya
Veterinary Officer
RVL, Pokhara
Presented by:
Dr Kedar Raj Pande
Senior Veterinary Officer
RVL, Pokhara
First Trimester Progress Report 2074/75
Regional Veterinary Laboratory, Pokhara
 First Quarter (Shrawan-Kartik) Progress
Report 2074/75
 Major laboratory services of RVL
 Progress on Good Governance
Program
Budget
head
ANNUAL
Budget
(000)
First quarter
progress
Weightage
Progress(%)
Financial
Progress(%)
Regular
(Working) 327013 14353 94.65% 75.58%
Regular
(Capital) 327014 5925
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Progress Summary
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S.
N
District Animal
Species
Sample
Received
Date Test
methods
Remarks
1 Animal
Quarantine
Office
Rupandehi
Goat 63 Ashoj ELISA,
Brucella
All Negative
2 Tanahu Cattle 28 Shrawan Serum
Biochemistry
Normal
3 Kapilvastu Goat 28 Kartik ELISA 24+ve 4-ve
4 Kapilvastu Goat 9 sharwan Penside test 6+ve 3-ve
5 Myagdi Buffalo 1 Kartik Giemsa
Staining
NAD
 PPR Vaccine
No. of vaccines Stock 2074/4/1 : 100000
No. distributed till Date 074/7/30: 7300
No. distributed till 1st quarter of 074/075: 7300
Remaining Stock: 92700
 Anti Rabies vaccine
No. of vaccines received till date: 3000
No. distributed till Date 074/7/30: 860
No. distributed till 1st quarter of 074/075: 860
Remaining Stock: 2140
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-
Positives: 150 for Coccidia (21.64%)
 Skin scrapings examination : 6 samples from dog
◦ 6 samples found Negative
 Total Sample Tested = 693 intestinal scrapings of poultry
Parasitological Examinations
Total EPG 90
Positive: 53 Negative : 37
• Site of sample collection: Thasang Rural Municipality
Mustang.
 Strongyloides 62.22% (56/90)
 Monezia 45.55% (41/90)
Parameters Mean ±SE
Total EPG 883.87±656.41
Strongyloides spp 614.63±468.80
Moniezia expansa 528.57±442.19
• Samples for testing Clinical Mastitis brought by
clients, and technicians for clinical
◦ SLST test Positive milk samples = 181 (67.03%)
◦ SLST test Negative Milk Samples = 89 (32.96%)
Total Sample Received : 270 animals
Microbiological Examinations...
* 33 Plastic bottled samples were not
cultured
43
16
12
3 3
9
0
5
10
15
20
25
30
35
40
45
50
E. Coli Streptococcus Staphylococcus Bacillus Fungus No growth
0
25
12
1
60
6
2
13
1 2
0 00
24
21
8
10
49
24
10
0 0 1 0
49
7 6
24
1
16
24
1 0 0 0 1
0
10
20
30
40
50
60
70
HS
MS
R
Diseases Total Percentage positive
Colibacillosis
129 15.63
CRD 117 14.18
Coccidiosis 114 13.81
Infectious Bursal Disease 98 11.87
Salmonellosis 37 4.48
Mycotoxicosis 36 4.36
Immunsupression 33 4.00
New Castle Disease 6 0.72
Omphalitis 34 4.12
Ascites 93 11.27
Avian Influenza 2 0.24
Others 126 15.27
Total cases (n) = 825
Post mortem and lab diagnosed poultry diseases
Species
Number of
Sample
Tested For
Test Method
Applied
Results
Goats 18 PPR
Pen side
test
12 +ve
Pig/Goat/ Dog 11 Rabies Rapid test All Negative
Poultry 69 IBD
IBDV rapid
test
28+ve
Poultry 38 Newcastle d/s
NDV rapid
test
1 +ve
Poultry 49 Avian Influenza
AIV rapid
test
2 +ve
2 H9
Virological Examinations
Species
Number of
Serum
Tested For
Test Method
Applied
Results
Cattle 51 Brucellosis RBPT All Negative
Buffalo 27 Brucellosis RBPT All Negative
Dog 4 Brucellosis RBPT All Negative
Poultry 80 Salmonellosis PAT 42 +ve
Poultry 80 Mycoplasmosis PAT 21 +ve
Serological Examinations
39
15
38
43
1
70
35
28
48
11
2 1 0 1 0 1
5
0
3
1
14
7
2
8
11
9
11
13
5
8
53
23
40
52
12
80
51
41
56
20
0
10
20
30
40
50
60
70
80
90
Noofsamples
Positive
Doubtful
Negative
Total
S.N. Calcium Phosphorus Magnesium Total Protein Glucose
1 1.283573 6.554622 1.4913 7.816513761 30.6122
2 7.969231 4.705882 0.9562 7.908256881 24.4897
3 6.553846 7.247899 1.5953 6.623853211 2.91545
4 6.307692 4.957983 1.5722 7.52293578 24.8554
5 7.446154 7.331933 1.526 7.779816514 30.6122
6 11.6 5.357143 1.7687 9.651376147 14.2857
7 8.215385 6.911765 2.3583 9.834862385 45.6647
8 8.553846 10.94538 1.919 11.1559633 41.6184
9 10.15385 5.420168 1.9421 9.871559633 22.1574
10 9.969231 8.004202 1.8497 9.266055046 18.0758
11 9.138462 6.281513 1.6994 9.211009174 40.8163
12 8.123077 10.2521 3.3872 12.34862385 13.1195
13 10.49231 8.193277 1.7572 9.431192661 8.45481
14 8.769231 5.882353 1.8034 7.926605505 8.45481
15 8.676923 8.55042 1.5953 7.963302752 30.3206
16 9.107692 7.710084 1.6531 10.03669725 25.3644
17 7.046154 4.747899 1.7687 7.394495413 19.2419
19 8.953846 4.852941 1.526 7.28440367 23.6151
20 9.6 5.39916 2.289 9.963302752 32.9446
21 8.307692 8.109244 2.3583 8.330275229 22.4489
22 10.64615 4.348739 1.2369 8.348623853 6.70553
23 7.753846 12.2479 2.0693 7.908256881 51.0204
24 8.307692 4.88 1.3872 13.35779817 13.7026
26 7.169231 5.798319 1.1907 7.724770642 20.6997
27 7.507692 6.134454 1.4219 8.752293578 30.3206
28 7.384615 6.386555 1.6184 8.568807339 18.9504
29 9.2 6.869748 1.1791 8.275229358 17.7842
30 8.984615 6.233894 1.7456 8.623853211 30.6122
SE 1.869029 1.951369 0.468861 1.52693373 11.817846
Mean 8.329358 6.796985 1.738054 8.888597641 23.923696
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First trimester report regional veterinary laboratory pokhara

  • 1.
    Prepared by: Dr. KrishnaPrasad Acharya Veterinary Officer RVL, Pokhara Presented by: Dr Kedar Raj Pande Senior Veterinary Officer RVL, Pokhara First Trimester Progress Report 2074/75 Regional Veterinary Laboratory, Pokhara
  • 2.
     First Quarter(Shrawan-Kartik) Progress Report 2074/75  Major laboratory services of RVL  Progress on Good Governance
  • 3.
    Program Budget head ANNUAL Budget (000) First quarter progress Weightage Progress(%) Financial Progress(%) Regular (Working) 32701314353 94.65% 75.58% Regular (Capital) 327014 5925 sfd e}/x]sf] e'Qmflg lbg af+ls Progress Summary
  • 4.
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  • 5.
    S. N District Animal Species Sample Received Date Test methods Remarks 1Animal Quarantine Office Rupandehi Goat 63 Ashoj ELISA, Brucella All Negative 2 Tanahu Cattle 28 Shrawan Serum Biochemistry Normal 3 Kapilvastu Goat 28 Kartik ELISA 24+ve 4-ve 4 Kapilvastu Goat 9 sharwan Penside test 6+ve 3-ve 5 Myagdi Buffalo 1 Kartik Giemsa Staining NAD
  • 6.
     PPR Vaccine No.of vaccines Stock 2074/4/1 : 100000 No. distributed till Date 074/7/30: 7300 No. distributed till 1st quarter of 074/075: 7300 Remaining Stock: 92700  Anti Rabies vaccine No. of vaccines received till date: 3000 No. distributed till Date 074/7/30: 860 No. distributed till 1st quarter of 074/075: 860 Remaining Stock: 2140
  • 7.
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  • 8.
    - Positives: 150 forCoccidia (21.64%)  Skin scrapings examination : 6 samples from dog ◦ 6 samples found Negative  Total Sample Tested = 693 intestinal scrapings of poultry Parasitological Examinations
  • 9.
    Total EPG 90 Positive:53 Negative : 37 • Site of sample collection: Thasang Rural Municipality Mustang.  Strongyloides 62.22% (56/90)  Monezia 45.55% (41/90) Parameters Mean ±SE Total EPG 883.87±656.41 Strongyloides spp 614.63±468.80 Moniezia expansa 528.57±442.19
  • 10.
    • Samples fortesting Clinical Mastitis brought by clients, and technicians for clinical ◦ SLST test Positive milk samples = 181 (67.03%) ◦ SLST test Negative Milk Samples = 89 (32.96%) Total Sample Received : 270 animals
  • 11.
    Microbiological Examinations... * 33Plastic bottled samples were not cultured 43 16 12 3 3 9 0 5 10 15 20 25 30 35 40 45 50 E. Coli Streptococcus Staphylococcus Bacillus Fungus No growth
  • 12.
    0 25 12 1 60 6 2 13 1 2 0 00 24 21 8 10 49 24 10 00 1 0 49 7 6 24 1 16 24 1 0 0 0 1 0 10 20 30 40 50 60 70 HS MS R
  • 13.
    Diseases Total Percentagepositive Colibacillosis 129 15.63 CRD 117 14.18 Coccidiosis 114 13.81 Infectious Bursal Disease 98 11.87 Salmonellosis 37 4.48 Mycotoxicosis 36 4.36 Immunsupression 33 4.00 New Castle Disease 6 0.72 Omphalitis 34 4.12 Ascites 93 11.27 Avian Influenza 2 0.24 Others 126 15.27 Total cases (n) = 825 Post mortem and lab diagnosed poultry diseases
  • 14.
    Species Number of Sample Tested For TestMethod Applied Results Goats 18 PPR Pen side test 12 +ve Pig/Goat/ Dog 11 Rabies Rapid test All Negative Poultry 69 IBD IBDV rapid test 28+ve Poultry 38 Newcastle d/s NDV rapid test 1 +ve Poultry 49 Avian Influenza AIV rapid test 2 +ve 2 H9 Virological Examinations
  • 15.
    Species Number of Serum Tested For TestMethod Applied Results Cattle 51 Brucellosis RBPT All Negative Buffalo 27 Brucellosis RBPT All Negative Dog 4 Brucellosis RBPT All Negative Poultry 80 Salmonellosis PAT 42 +ve Poultry 80 Mycoplasmosis PAT 21 +ve Serological Examinations
  • 16.
    39 15 38 43 1 70 35 28 48 11 2 1 01 0 1 5 0 3 1 14 7 2 8 11 9 11 13 5 8 53 23 40 52 12 80 51 41 56 20 0 10 20 30 40 50 60 70 80 90 Noofsamples Positive Doubtful Negative Total
  • 17.
    S.N. Calcium PhosphorusMagnesium Total Protein Glucose 1 1.283573 6.554622 1.4913 7.816513761 30.6122 2 7.969231 4.705882 0.9562 7.908256881 24.4897 3 6.553846 7.247899 1.5953 6.623853211 2.91545 4 6.307692 4.957983 1.5722 7.52293578 24.8554 5 7.446154 7.331933 1.526 7.779816514 30.6122 6 11.6 5.357143 1.7687 9.651376147 14.2857 7 8.215385 6.911765 2.3583 9.834862385 45.6647 8 8.553846 10.94538 1.919 11.1559633 41.6184 9 10.15385 5.420168 1.9421 9.871559633 22.1574 10 9.969231 8.004202 1.8497 9.266055046 18.0758 11 9.138462 6.281513 1.6994 9.211009174 40.8163 12 8.123077 10.2521 3.3872 12.34862385 13.1195 13 10.49231 8.193277 1.7572 9.431192661 8.45481 14 8.769231 5.882353 1.8034 7.926605505 8.45481 15 8.676923 8.55042 1.5953 7.963302752 30.3206 16 9.107692 7.710084 1.6531 10.03669725 25.3644 17 7.046154 4.747899 1.7687 7.394495413 19.2419 19 8.953846 4.852941 1.526 7.28440367 23.6151 20 9.6 5.39916 2.289 9.963302752 32.9446 21 8.307692 8.109244 2.3583 8.330275229 22.4489 22 10.64615 4.348739 1.2369 8.348623853 6.70553 23 7.753846 12.2479 2.0693 7.908256881 51.0204 24 8.307692 4.88 1.3872 13.35779817 13.7026 26 7.169231 5.798319 1.1907 7.724770642 20.6997 27 7.507692 6.134454 1.4219 8.752293578 30.3206 28 7.384615 6.386555 1.6184 8.568807339 18.9504 29 9.2 6.869748 1.1791 8.275229358 17.7842 30 8.984615 6.233894 1.7456 8.623853211 30.6122 SE 1.869029 1.951369 0.468861 1.52693373 11.817846 Mean 8.329358 6.796985 1.738054 8.888597641 23.923696
  • 18.
     ufO{ e}l;sf]af+emf]kg lgjf/0f lzlj/df gd’gf ;+sng tyf kl/If0f M NLBC ;+u ;xsfo{ ug{’kg]{ ePsf]n]  cw{aflif{s O{lkl8ldof]nf]lhsn a’n]l6g k|sfzg MVEC af6 gcfPsf]  /fli6o lk=lk=cf/=vf]/]t,:jfO{g lkme/ tyf /flgv]t /f]u lgoGq0f sfo{qmd cGtu{t l;/f]dlg6l/8sf] nflu gd’gf ;+sng tyf k|]if0f M EoflS;g gnufPsf]  cfwf/e’t k|of]uzfnfx?sf] :t/f]Ggltsf nflu k|lalwx:tfGt/0f,cg’udg tyf lgl/If0f M lhNnf :t/ k|of]uzfnf lgdf{0f ;DkGg e} g;s]sf]n]  cGt/ k|of]uzfnf cjnf]sg e|d0f M r’gfjn] ubf{
  • 19.
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  • 20.
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  • 21.

Editor's Notes

  • #7 Neja rab single dose vaccines: expired
  • #12 E.coli and Streptoococous are the major bacteria isolated in clinical mastitis milk samples