This document outlines procedures for synthesizing new organic compounds using boronic acids and characterizing the compounds. It describes calculating and measuring reactants, combining the reactants in solution, isolating precipitated products, and analyzing products using techniques like NMR spectroscopy, melting point determination, TLC, and FT-IR spectroscopy to determine structures and properties. Examples are given of three projects where new compounds were synthesized, isolated in good yields, and characterized using these methods.
HPLC is Analytical technique that is used for separating the mixture of substances,so there is a number of promising application of HPLC-UV here uv detector is used which record the absorbance
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Dr.S.Karthikumar
Asst. Prof., Dept. of Biotechnology
Kamaraj College of Engineering and Technology
S.P.G.C.Nagar, Virudhunagar, Tamilnadu, India
skarthikumar@gmail.com
HPLC is Analytical technique that is used for separating the mixture of substances,so there is a number of promising application of HPLC-UV here uv detector is used which record the absorbance
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Dr.S.Karthikumar
Asst. Prof., Dept. of Biotechnology
Kamaraj College of Engineering and Technology
S.P.G.C.Nagar, Virudhunagar, Tamilnadu, India
skarthikumar@gmail.com
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
Chromatography is an analytical method in which compounds are physically separated and measured.
The main purpose of chromatography is to separate and quantify the target sample.
The Chromatography technique used to separate a mixture of compounds in pharmaceutical sciences , analytical analytical Chemistry with the purpose of identifying, quantifying and purifying the individual components of the mixture.
Metabolomic Profiling of Spent Biomass Of Marine Microalgae, Chlorella vulgarispriyanka raviraj
OBJECTIVE:
To evaluate the presence of any high value added compounds in the spent biomass of C. vulgaris
To identify the biological activity of the extracted compounds
To evaluate the structure and nature of the compounds using Nuclear Magnetic Resonance Spectroscopy and other analytical techniques.
Development of economically viable methodologies for the simultaneous extraction of by-products from a single set of biomass.
biological activities performed -Total antioxidant capacity, Anti bacterial activity, Anti-tuberculosis activity, Anti proliferative assay
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC)ShreyaBhatt23
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY workflow, types of hplc, normal phase HPLC, reverse phase HPLC,types of column, advantages pf HPLC over other liquid chromatography, parameters of HPLC. SOURCE: ARPAN YOUTUBE
• It is the combination of liquid chromatography and the mass spectrometry.
• Liquid chromatography-mass spectrometry (LC-MS) is an analytical chemistry
technique that combines the physical separation capabilities of liquid
chromatography with the mass analysis capabilities of mass spectrometry.
• The combination of these two powerful techniques gives the chemical analyst the
ability to analyze virtually any molecular species; including, thermally labile, non
volatile, and high molecular weight species.
This presentation was presented when I was MVSc 1st year student, hence this ppt will provide a piece of basic information about the use of Chromatography.in toxicology. Principle of its work and related terminology are explained in this ppt.
Presented By :- Raghav Sharma
Class :- M. Pharm, 1st sem.
Department :- Pharmaceutics
Institute :- Parul Institute of Pharmacy
Content :-
Instrumentation and working of flame photometry
Flame atomizer
Nebulizer
Atomizer burner
Monochromator
Detector
Amplifier
Advantages
Disadvantages
Reference
Mass Spectrometry Applications and spectral interpretation: Basics
Hannah poster
1. Introduction
Organic chemists synthesize new
chemical compounds in order to gain a
better understanding of the structures
of different molecules. This
understanding can help chemists
develop new molecules with qualities
that are structure-specific.
Synthesizing new organic
compounds using boronic acids
Analyzing and determining
structures of new synthetic
organic compounds to determine
if they have any unique properties
using melting point, 1
H NMR
spectra, 13
C NMR spectra , TLC,
FT-IR spectra, and UV-VIS spectra
Characterizing the new
compounds based on the
structure and the unique qualities
of the compound
Objectives
Calculate and measure out 2 mmol
of each of the reactants based on
the molecular weight from the
bottle or from the chemical catalog
Collect 2 clean 50-ml beakers and
add each of the reactants to its own
respective beaker under the flow
hood
Dissolve each reactant in 10 mL
50% aqueous ethanol, using heat if
necessary to drive all of the solvent
into solution.
Simultaneously, Pasteur pipet equal
amounts of both solutions into a
third beaker, ensuring that equal
amounts of reactant are delivered
to the solution at relatively the
same time
Let solution sit in the flow hood
overnight until almost completely
evaporated and the product has
completely fallen out of solution
The next day, vacuum filter out
precipitate from solution using ice-
cold deionized water to suspend
the product
Measure product in order to
calculate a percent yield of product
based on the predicted molecular
weight
Prepare NMR tubes with samples of
the product and analyze under
proton and carbon NMR to help
predict the structure of the product
obtained.
Take TLC to test whether the
substance is fluorescent under UV
light
General Procedures
Projects
M.P. (O
C) Yield 1
H NMR 13
C NMR Appearnce
50 195-196 80% 9/9 H peaks 13/14 C peaks White, fluffy, crystals
51 >260 84% 10/10 H
peaks
Not soluble
enough
Fine, yellow powder
54 >260 88% 11/11 H
peaks
16/18 C peaks Fine, light yellow
powder
Methods of Characterization and Lab Techniques
Thin Layer Chromatography (TLC) A plate coated with silica gel separates out molecules
in a sample. TLC served as an early indicator of purity in a compound. It also provides an
instrument through which fluorescence can be demonstrated by placing the plate under
long-wave UV light.
1
H Nuclear Magnetic Resonance (NMR) Spectra A spectrum used to measure the amount
of hydrogen atoms present in a molecule. The NMR machine records in parts per million
the locations of each of the different hydrogen atoms in the molecules using magnetic
fields aimed in different directions.
13
C NMR Spectra A spectrum used to measure and locate the different carbon atoms
within the molecule. The NMR instrument can be used to confirm possible molecular
structures of new compounds.
Melting Point Apparatus The machine that measures melting points helps characterize
compounds and can be an indication of purity in a compound. A smaller melting point
range supports a more pure compound compared to a larger melting point range which
leads to a less pure compound. The highest temperature that can be measured was 260O
C.
Rotary Evaporator The rotary evaporator uses heat, rotation, and a vacuum to quickly dry
out and isolate a sample from a solution.
Fourier Transfer Infrared Spectroscopy (FT-IR) This machine measures infrared
absorbencies within the bonds of the molecules in the compound. This method can help
researchers identify certain specific chemical bonds in a compound.
UV-VIS The UV-VIS machine measures absorbencies of the compound in the UV and visual
wavelength range. The lambda max of the spectrum helps to characterize a new
compound.
Column Chromatography One of the methods of purifying a mixture of compounds is
column chromatography. In this filtration system, the mixture travels through silica gel,
separating out larger molecules from faster-travelling smaller molecules.
Recrystallization Another method of purification of a compound, recrystallization is a
process where a mixture of compounds is driven into a solution with heat until completely
dissolved and then allowed to cool until the desired product falls out of solution and
recrystallizes.
Special Thanks to:
Dr. Groziak
Aurora, Brian, Dung, Elena, Eric, Tesfay
Dr. Michele Parker, Dr. Katy Korsmeyer, and Mary Lou Stuart
SV-STEM program
California State University East Bay
Highlights
Learning how to use the NMR
machine for carbon and proton
spectra and being able to analyze
the data
Working in a real-world lab setting