an attempt to provide brief data on the topic!

1. By: anjali p. parab HAIRY ROOT CELL CULTURE

2. HAIRY ROOT CELL CULTURE

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5. This processes take advantage of the naturally occurring hairy root disease in Dicotyledons.3/30/2011 4

7. Autotrophy in plant hormones

8. Fast growth

9. High levels of secondary metabolite production3/30/2011 5

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11. Production of hairy roots in vivo: Agrobacterium recognizes some signal molecules exuded by wounded plant cells and becomes attached to it. The bacteria contain the Root inducing plasmid (Ri-plasmid) The bacteria genetically transfer part of the Ri-plasmid called the transfer DNA (T-DNA) to the plant genome, where it gets expressed and make the plant cell to: 3/30/2011 7

12. Production of hairy roots in vivo: Proliferate by increasing the rate of cell division (cytokine expression) and cell elongation (auxin expression) to produce the hairy roots. Produce the opines which is a type of unusual amino acids (octopine, agropine,nopaline, mannopine, and cucumopine) which is used by the bacterium as a carbon, nitrogen and energy source. 3/30/2011 8

13. THE PROCESS………… Plantcell Agrobacterium cell Ri-plasmid 3/30/2011 9

14. Ri-Plasmid Ri-Plasmid 3/30/2011 10

15. Induction of hairy root cultures in vitro: Explants are wounded and then inoculated with Agrobacteriumrhizogenes. Usually two or three days later, the explant can be transferred into solid media with antibiotics, such as cefotaxime, vancomycin or ampicillin to kill or eliminate redundant bacteria. The hairy roots will be induced within a short period of time, which varies from one week to over a month depending on different plant species. The decontaminated hairy roots can be subcultured on phytohormone-free medium. 3/30/2011 11

17. Air lift bioreactor

18. Bubble column bioreactor

19. Turbine blade bioreactor

20. Mist (Trickle bed) bioreactor

21. Rotating drum bioreactor

22. Spin filter bioreactor3/30/2011 12

23. Stirred tank reactor with suspension culture of Helianthus annuus Bubble column with Hairy roots of B. vulgaris 3/30/2011 13

24. Bubble column with Hairy roots of Harpagophytumprocumbens 3/30/2011 14

25. Agar plate with Hairy roots of Beta vulgaris (beetroot) 3/30/2011 15

26. Advantages of hairy root cultures: The hairy root system is genetically and biosynthetically stable High production of secondary metabolites. The culture can grow under phyto-hormone-free conditions. The culture shows fast growth which reduces the culture time and easy the handling 3/30/2011 16

27. Application of hairy root cultures: Functional analysis of genes. Expressing foreign proteins. Production of secondary metabolites. The culture may produce compounds which is not found in untransformed roots. The culture may change the composition of metabolites. The culture can be used to regenerate a whole plant. 3/30/2011 17

29. Shikonin: used as an anti-bacterial and anti-ulcer agent

30. Anthraquinone: used for dyes and medicinal purpose

31. Opiate alkaloids: particularly codeine and morphine for medical purposes

32. Berberine: an alkaloid with medicinal uses for cholera and bacterial dysenterry

33. Valepotriates: used as a sedative

34. Ginsenosides: for medicinal purposes

35. Rosmarinic acid: for antiviral, suppression of endotoxin shock and other medicinal purposes

36. Quinine: for malaria

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40. 2)Plant tissue culture by Bhojwani & Razdan

41. 3)www.springerlink.com

42. 4)www.420magazine.com3/30/2011 21

43. THANK YOU! 3/30/2011 22