This document summarizes the biogenic synthesis of zinc oxide nanoparticles using papaya leaf extract. The objectives are to characterize the synthesized ZnO NPs using UV-Vis spectroscopy and XRD, and to synthesize them through a green pathway using papaya leaf extract. Zinc acetate and ammonium carbonate solutions were added dropwise to the extract to form a precipitate, which was filtered, dried, and calcined. XRD characterized the crystalline size as 19.22 nm, while UV-Vis determined the band gap energy to be 3.3406 eV.

1. BIOMEDIATED SYNTHESIS OF ZINC OXIDE
NANOPARTICLES USING CARICA PAPAYA AND ITS
CHARACTERIZATION
submitted by,
Christy Mary Joy

2. Application of ZnO NPs
• Rubber Industry.
• Pharmaceutical and cosmetic industry.
• Photo catalysis.
• Miscellaneous applications.
• Electronics and electro technology industries.
• Textile industry.

3. OBJECTIVES OF STUDY
1. To characterize synthesized ZnO NPs using ultraviolet-visible
(u-V) spectroscopy, X- ray Diffraction (XRD).
2. To synthesize ZnO NPs through a green synthesis pathway
using Carica papaya (papaya Leaf).

4. Methodology
Preparation of standard solution of zinc acetate:
Preparation of standard solution of Ammonium Carbonate:

5. PROCEDURE
• 100 ml of leaf extract was taken in a 500 ml clean beaker mounted on a magnetic
stirrer. 100 ml of 0.1 M ammonium carbonate solution and zinc acetate solutions
were simultaneously added to it from a burette drop by drop at the rate of one drop
in 2 seconds.
• During the addition, the solution was stirred vigorously about 2-3 hours till a
greenish precipitate was formed. The inert medium provided by papaya leaf extract
isolates the particles formed from aggregation.
• The precipitate is filtered off using funnel and filter paper and washed with distilled
water several times. It was then dried and transferred into a crucible and heated for
two hours at 400 ˚C in a muffle furnace . The sample was then transferred into a
mortar and grinded well .

6. • X-ray diffraction (XRD)
crystalline size of synthesized Zinc oxide particle using Scherer's
equation
D= 𝐾 λ /𝛽 𝑐𝑜𝑠Ө
where, β =𝐹𝑊𝐻𝑀ax 2*𝜋/180°
• UV-Visible spectroscopy
Band gap energy
Ebg = hc / λ
CHARACTERIZATION TECHNIQUES

7. RESULTS AND DISCUSION
 Characterization of Synthesized Zinc Oxide Nanoparticles
• In this work Zinc oxide nanoparticles are synthesized through biogenic green
method using Zinc acetate and ammonium carbonate as precursor and
papaya leaf as capping agent.
• The synthesized ZnO NPs was characterized accordingly using XRD, UV-V
Spectroscopy.

8. X-ray Diffraction (XRD)
XRD of synthesized ZnO nanoparticles

9. UV Visible Analysis
UV-Visible spectrum of synthesized ZnO NPs

10. CONCLUSION:
 The crystalline size of synthesized ZnO NP = 19.22 nm
 The energy band gap of as synthesized nanoparticles was
obtained to be equal to 3.3406 eV.