1) The CIAT germplasm bank holds over 67,000 bean accessions, 33 cassava accessions, and over 23,000 tropical forage accessions, which are distributed internationally to benefit food security.
2) CIAT works with ICA to ensure germplasm exports are free of quarantine pests through disease testing and indexing. Research is conducted to improve health, such as managing ergot disease in Brachiaria.
3) Molecular diagnostic methods have been standardized to detect viruses in cassava, allowing detection of multiple viruses from one sample and improving over use of indicator plants. This benefits distribution of virus-free material.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMilliporeSigma
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Dr. Stephanie Rossow - Applications of Next Generation SequencingJohn Blue
Applications of Next Generation Sequencing - Dr. Stephanie Rossow, College of Veterinary Medicine, University of Minnesota, from the 2016 Allen D. Leman Swine Conference, September 17-20, 2016, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
Emerging Viral Risks and Mitigation Strategies in Biologics ManufacturingMilliporeSigma
Emerging viruses represent a constant challenge to biopharmaceutical manufacturers, and therefore formal risk assessments and informed programs of safety testing are necessary to assure safety. Emerging viruses such as the Zika virus have the potential to contaminate raw materials of human origin, Schmallenberg virus is a contaminant of bovine serum, and the long-known, but often ignored, Hepatitis E virus represents further challenges to the safety of raw materials. Results of in vitro culture and molecular testing strategies of raw materials for viruses with diverse characteristics will be presented, and holistic approaches to mitigate the risk of novel viruses to the safety of raw materials will be outlined.
In this webinar, you will learn:
-The identity of emerging viruses and potential impact on the safety of raw materials and final products
-Testing strategies for specific viruses
-Holistic approaches to mitigate the risk of novel viruses in raw materials
Next generation sequencing for Identification and Characterization of plant v...Malyaj R Prajapati
Plant viruses have always been a challenge to plant growth and crop production in several parts of the world. Virus can be transmitted by vegetative propagation, fungi, nematodes, aphids, leaf hoppers, plant hoppers, beetles, white flies, and so forth. Viruses symptoms vary with the infecting virus and the infected part which includes leaf spots, leaf blights, root rots, fruit rots, fruit spots, wilt, dieback and decline. It is causing economic losses by reducing crop quality, quantity and nutritional value. Thus, their reliable detection is of a crucial importance for plant protection. While the adoption of molecular techniques such as RT-PCR has increased the speed and accuracy of virus diagnostics, such techniques only allow the detection of known viruses, i.e., each test is specific to one or a small number of related viruses. Therefore, unknown virus can be missed and testing can be slow and expensive if molecular tests are unavailable. NGS technology is one of the most popular tools for virus diagnostics. It is highly efficient, rapid diagnostics tools, and low-cost high-throughput and deep RNA sequencing. Due to the capacity to target multiple unique signature loci of virus in an infected plant metagenome and also useful for discovery of new virus and new hosts. It is including virus genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. By using deep RNA-seq requires fast and robust bioinformatics methods to enable host sequence removal and virus classification. Future developments in this area, including the use of bioinformatics tools for identification and characterization of multiple plant virus and analysis of diversity of plant viruses.
Viral Risk Mitigation Strategies: Key Considerations in the Prevention and De...MilliporeSigma
Regulatory guidelines have defined industry best practices around adventitious virus contamination and risk mitigation in terms of patient safety.
Today, the industry is taking a closer look at minimizing the business risk associated with viral contamination and is taking a more directed view of risk mitigation. This approach includes virus prevention and detection, in addition to removal.
From cell culture seed train to final fill vial, this presentation will describe:
-Potential risks associated with different areas of biotech processes
-What can be done to minimize adventitious virus risk in those areas.
The overarching strategy of risk mitigation will include evaluation of raw materials, modified expression systems, environmental controls, upstream and downstream processing, as well as testing and regulatory considerations.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMilliporeSigma
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Dr. Stephanie Rossow - Applications of Next Generation SequencingJohn Blue
Applications of Next Generation Sequencing - Dr. Stephanie Rossow, College of Veterinary Medicine, University of Minnesota, from the 2016 Allen D. Leman Swine Conference, September 17-20, 2016, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
Emerging Viral Risks and Mitigation Strategies in Biologics ManufacturingMilliporeSigma
Emerging viruses represent a constant challenge to biopharmaceutical manufacturers, and therefore formal risk assessments and informed programs of safety testing are necessary to assure safety. Emerging viruses such as the Zika virus have the potential to contaminate raw materials of human origin, Schmallenberg virus is a contaminant of bovine serum, and the long-known, but often ignored, Hepatitis E virus represents further challenges to the safety of raw materials. Results of in vitro culture and molecular testing strategies of raw materials for viruses with diverse characteristics will be presented, and holistic approaches to mitigate the risk of novel viruses to the safety of raw materials will be outlined.
In this webinar, you will learn:
-The identity of emerging viruses and potential impact on the safety of raw materials and final products
-Testing strategies for specific viruses
-Holistic approaches to mitigate the risk of novel viruses in raw materials
Next generation sequencing for Identification and Characterization of plant v...Malyaj R Prajapati
Plant viruses have always been a challenge to plant growth and crop production in several parts of the world. Virus can be transmitted by vegetative propagation, fungi, nematodes, aphids, leaf hoppers, plant hoppers, beetles, white flies, and so forth. Viruses symptoms vary with the infecting virus and the infected part which includes leaf spots, leaf blights, root rots, fruit rots, fruit spots, wilt, dieback and decline. It is causing economic losses by reducing crop quality, quantity and nutritional value. Thus, their reliable detection is of a crucial importance for plant protection. While the adoption of molecular techniques such as RT-PCR has increased the speed and accuracy of virus diagnostics, such techniques only allow the detection of known viruses, i.e., each test is specific to one or a small number of related viruses. Therefore, unknown virus can be missed and testing can be slow and expensive if molecular tests are unavailable. NGS technology is one of the most popular tools for virus diagnostics. It is highly efficient, rapid diagnostics tools, and low-cost high-throughput and deep RNA sequencing. Due to the capacity to target multiple unique signature loci of virus in an infected plant metagenome and also useful for discovery of new virus and new hosts. It is including virus genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. By using deep RNA-seq requires fast and robust bioinformatics methods to enable host sequence removal and virus classification. Future developments in this area, including the use of bioinformatics tools for identification and characterization of multiple plant virus and analysis of diversity of plant viruses.
Viral Risk Mitigation Strategies: Key Considerations in the Prevention and De...MilliporeSigma
Regulatory guidelines have defined industry best practices around adventitious virus contamination and risk mitigation in terms of patient safety.
Today, the industry is taking a closer look at minimizing the business risk associated with viral contamination and is taking a more directed view of risk mitigation. This approach includes virus prevention and detection, in addition to removal.
From cell culture seed train to final fill vial, this presentation will describe:
-Potential risks associated with different areas of biotech processes
-What can be done to minimize adventitious virus risk in those areas.
The overarching strategy of risk mitigation will include evaluation of raw materials, modified expression systems, environmental controls, upstream and downstream processing, as well as testing and regulatory considerations.
Genomic surveillance of the Rift Valley fever: From sequencing to Lineage ass...ILRI
Poster prepared John Juma, Vagner Fonseca, Samson Limbaso, Peter van Heusden, Kristina Roesel, Bernard Bett, Rosemary Sang, Alan Christoffels, Tulio de Oliveira and Samuel Oyola for the Kenya One Health Online Conference, 6-8 December 2021
Does your cell line have a secret? Avoid surprises with characterizationMerck Life Sciences
Watch the recording of this webinar here: https://bit.ly/2Y05bV4
The first step to avoiding an unpleasant and costly contamination event is characterization of your cell banks.
Regardless of the biotech product, careful characterization of the cell banks used in its production is the first step in mitigating the risk of a contamination event. In fact, cell line characterization is an important component of the overall viral safety strategy for the product. We will describe the testing necessary to ensure cell banks are free from infectious and other adverse agents and that meets current regulatory expectations. Different levels of testing are performed for master, working, and end of production cell banks, and the differences in testing for each of these types of banks will be discussed.
In this webinar, you will learn:
• The types of tests that are needed to fully characterize your cell banks
• The best tests to use for your particular cell line
• Reasons why a viral contaminant may be missed
Presentation 6: Vibrio parahaemolyticus: genome plasticity, mobile genetic el...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Presentation 18: Problems other than AHPND in EMS ponds, including the micros...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Setting up for successful lot release testing by Edmund AngMilliporeSigma
Is your lot release testing strategy ready for global commercialization?
In this webinar, you will learn:
• CMC testing requirements with CHO production platform for global commercialization
• Lot release testing of product intermediates and final product
• Product-specific qualification study
• Alternative rapid testing methods to advance lot release testing
CHO cells continue to serve as a key cell substrate for the manufacturing of recombinant proteins that span beyond therapeutic monoclonal antibodies and including subunit vaccines.
In this presentation, we will cover the CMC testing requirements with CHO production platform for global commercialization, Lot release testing of product intermediates and final product, product-specific qualification study and highlight the application of new testing methods and the benefits they bring to advance Lot Release Testing.
Platform Technologies to Accelerate Novel Vaccine Development and ManufacturingMerck Life Sciences
Watch the presentation of this webinar here: https://bit.ly/3jmLYHu
State-of-the-art vaccine technologies are transforming vaccine development, and solutions for fast and reliable production are needed.
The vaccine industry has undergone a revolution in technology resulting in a variety of novel therapeutic platforms that accelerate development and significantly reduce the duration for process optimization and scale-up. However, challenges in maintaining efficacy and improving process robustness remain. In this presentation, we present a comparison of these novel technologies, discuss key considerations for manufacturing and share selected case studies for platforms such as virus-like-particles, viral vectors, plasmid DNA, and mRNA platform.
In this webinar, you will learn:
• Benefits of platform technologies in vaccine development
• Key considerations when deciding between platforms
• Vaccine pipeline analysis and selected case studies
Presented by:
David Loong, Ph.D, Senior Consultant, Novel Modalities Asia Pacific, Bioprocessing Strategy
Josephine Cheng, Senior Consultant, Core Modalities Asia Pacific, Bioprocessing Strategy
Genomic surveillance of the Rift Valley fever: From sequencing to Lineage ass...ILRI
Poster prepared John Juma, Vagner Fonseca, Samson Limbaso, Peter van Heusden, Kristina Roesel, Bernard Bett, Rosemary Sang, Alan Christoffels, Tulio de Oliveira and Samuel Oyola for the Kenya One Health Online Conference, 6-8 December 2021
Does your cell line have a secret? Avoid surprises with characterizationMerck Life Sciences
Watch the recording of this webinar here: https://bit.ly/2Y05bV4
The first step to avoiding an unpleasant and costly contamination event is characterization of your cell banks.
Regardless of the biotech product, careful characterization of the cell banks used in its production is the first step in mitigating the risk of a contamination event. In fact, cell line characterization is an important component of the overall viral safety strategy for the product. We will describe the testing necessary to ensure cell banks are free from infectious and other adverse agents and that meets current regulatory expectations. Different levels of testing are performed for master, working, and end of production cell banks, and the differences in testing for each of these types of banks will be discussed.
In this webinar, you will learn:
• The types of tests that are needed to fully characterize your cell banks
• The best tests to use for your particular cell line
• Reasons why a viral contaminant may be missed
Presentation 6: Vibrio parahaemolyticus: genome plasticity, mobile genetic el...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Presentation 18: Problems other than AHPND in EMS ponds, including the micros...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Setting up for successful lot release testing by Edmund AngMilliporeSigma
Is your lot release testing strategy ready for global commercialization?
In this webinar, you will learn:
• CMC testing requirements with CHO production platform for global commercialization
• Lot release testing of product intermediates and final product
• Product-specific qualification study
• Alternative rapid testing methods to advance lot release testing
CHO cells continue to serve as a key cell substrate for the manufacturing of recombinant proteins that span beyond therapeutic monoclonal antibodies and including subunit vaccines.
In this presentation, we will cover the CMC testing requirements with CHO production platform for global commercialization, Lot release testing of product intermediates and final product, product-specific qualification study and highlight the application of new testing methods and the benefits they bring to advance Lot Release Testing.
Platform Technologies to Accelerate Novel Vaccine Development and ManufacturingMerck Life Sciences
Watch the presentation of this webinar here: https://bit.ly/3jmLYHu
State-of-the-art vaccine technologies are transforming vaccine development, and solutions for fast and reliable production are needed.
The vaccine industry has undergone a revolution in technology resulting in a variety of novel therapeutic platforms that accelerate development and significantly reduce the duration for process optimization and scale-up. However, challenges in maintaining efficacy and improving process robustness remain. In this presentation, we present a comparison of these novel technologies, discuss key considerations for manufacturing and share selected case studies for platforms such as virus-like-particles, viral vectors, plasmid DNA, and mRNA platform.
In this webinar, you will learn:
• Benefits of platform technologies in vaccine development
• Key considerations when deciding between platforms
• Vaccine pipeline analysis and selected case studies
Presented by:
David Loong, Ph.D, Senior Consultant, Novel Modalities Asia Pacific, Bioprocessing Strategy
Josephine Cheng, Senior Consultant, Core Modalities Asia Pacific, Bioprocessing Strategy
Strategies for controlling viral diseases in ornamental crops 1Henny Takhellambam
This ppt will give a brief idea about how to control viral diseases in ornamental crops. Viral diseases are difficult to control as there are no specific chemicals developed to control viral diseases to date. So, many scientists developed several strategies to decrease the disease spread; the strategies are mentioned in the ppt.
Helping Farmers to Grow Healthier Crops: Use of qPCR to Monitor Plant Resista...Jonathan Clarke
1. Importance of crop diseases
2. Disease control measures
3. Use of qPCR in selected pathosystems:
• Downy mildew of opium poppy
• Verticillium wilt of olives
Looking for insights into current global regulatory expectations for viral safety? Read the special report from BioProcess International, in collaboration with Martin Wisher, Senior Regulatory Consultant focusing on BioReliance biosafety® services.
Viral Risk Mitigation - A Global Regulatory PerspectiveMilliporeSigma
Looking for insights into current global regulatory expectations for viral safety? Read the special report from BioProcess International, in collaboration with Martin Wisher, Senior Regulatory Consultant focusing on BioReliance biosafety® services.
Durante la Semana de la Agricultura y la Alimentación, el Programa de Investigación del CGIAR en Cambio Climático, Agricultura y Seguridad Alimentaria – CCAFS, la Organización de las Naciones Unidas para la Alimentación y la Agricultura, FAO, y el Centro Internacional de Agricultura Tropical – CIAT, apoyaron la II Reunión Internacional de Ministros y altas autoridades de agricultura sobre agricultura sostenible y cambio climático con un documento base y su presentación sobre los retos que representa el cambio climático para la agricultura en Latino América y el Caribe.
Taller sobre intervenciones en nutrición, género y agricultura: situación actual y oportunidades futuras’, organizado por el CIAT y HarvestPlus en Ciudad de Guatemala. Leer más: http://ow.ly/XNIv30mGYBv
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...CIAT
Taller sobre intervenciones en nutrición, género y agricultura: situación actual y oportunidades futuras’, organizado por el CIAT y HarvestPlus en Ciudad de Guatemala. Leer más: http://ow.ly/XNIv30mGYBv.
Presentado por Byron Reyes, CIAT/ Harvestplus
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...CIAT
Taller sobre intervenciones en nutrición, género y agricultura: situación actual y oportunidades futuras’, organizado por el CIAT y HarvestPlus en Ciudad de Guatemala. Leer más: http://ow.ly/XNIv30mGYBv
El rol de los padres en la nutrición del hogarCIAT
Taller sobre intervenciones en nutrición, género y agricultura: situación actual y oportunidades futuras’, organizado por el CIAT y HarvestPlus en Ciudad de Guatemala. Leer más: http://ow.ly/XNIv30mGYBv
Jennifer Twyman, Líder de investigación de Género en el CIAT
Scaling up soil carbon enhancement contributing to mitigate climate changeCIAT
The 4 per 1000 Africa Symposium - Building synergies across Africa to advance on soils for food security and climate, Johannesburg, South Africa 24-26 October 2018
Rolf Sommer, Kristin Piikki, Mats Söderström, Sylvia Nyawira, Mayesse da Silva, Wuletawu Abera and
Job Kihara
Impacto del Cambio Climático en la Agricultura de República DominicanaCIAT
El Banco Interamericano de Desarrollo (BID) y el Centro Internacional de Agricultura Tropical (CIAT), con el apoyo de los Programas de Investigación de CGIAR sobre Políticas, Instituciones y Mercados (PIM) y sobre Cambio Climático, Agricultura y Seguridad Alimentaria (CCAFS), se han asociado para comprender, a través de la ciencia, el impacto del cambio climático en cultivos claves y el impacto económico en la productividad de la agricultura en países de ALC.
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...CIAT
BioTerra es un sistema innovador de monitoreo de la biodiversidad y sus amenazas desarrollado por el Programa Riqueza Natural de la Agencia de los Estados Unidos para el Desarrollo Internacional (USAID), y sus socios locales – el Centro Internacional de Agricultura Tropical (CIAT) y el Instituto Alexander von Humboldt (IAvH) – para apoyar al gobierno colombiano en el cumplimiento de las metas y compromisos de conservación de la biodiversidad. Este sistema busca complementar y aunar esfuerzos existentes de monitoreo de la biodiversidad y sus amenazas, a nivel nacional y regional.
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue in Colo...CIAT
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
Tackling cadmium in cacao and derived products – from farm to forkCIAT
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...CIAT
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
Geographical Information System Mapping for Optimized Cacao Production in Col...CIAT
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoterasCIAT
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
El taller ‘Cacao libre de cadmio’, organizado por el CIAT, CIRAD, y la AFD, se lleva a cabo del 12 al 14 de marzo en la sede del CIAT en Palmira,y tiene como objetivo integrar un consorcio de actores y disciplinas claves de la región, así como elaborar un proyecto de investigación aplicada que dé respuesta a este problema que afecta a los cacaoteros de Colombia, Perú y Ecuador. http://ow.ly/J43p30iU0UZ
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. Outline
1. The context: global collections
2. Key concepts around plant quarantine; cooperation CIAT-ICA
3. Health controls in the lab for CIAT Programs
4. Research to improve health in germplasm exports: 3 case studies
5. Perspectives: the lab towards certification
3. Holdings in Germplasm at CIAT
Registered into the International Treaty
(Agreement signed with the governing body on October 16, 2006)
Crops
Taxa
( No.)
Accessions
(No.)
Beans (Phaseolus)
Cassava (Manihot)
Tropical Forages
37,794
6,643
23,140
67.577
33
Number
(No.)
1
1
1
45
734
Origin Country
(No.)
112
28
75
Germplasm materials conserved/ distributed as International Public Goods
Source: GRP – CIAT, 2014
812
The International Treaty on Plant
Genetic Resources for Food and
Agriculture
4. What do we distribute?
Distribution of germplasm by the GRP in 1973-2013
Forage: 88,835 samples
109 countries
Bean: 431,203 samples
105 countries
Cassava: 37,230 samples
80 countries
Total samples distributed : 557,268 Source: GRP – Dec. 2014
5. Germplasm Selection and Process Order
Search
List of germplasm
Add to Cart
Display order registration
Select purpose
Select
Database
Method of acceptance
Accept the Material
Transfer Agreement
No accept
stop Decision
Accept
If select
on SMTA
Send request
Send email about request
to receptor and to
CIAT
Database
Save order
Display
SMTA
Information
A.Hernandez, 2014
6. O.Rivera, 2014
Material distributed over the last ten years
Countries receveing
materials from GRP
Countries no
distribution
7. Some concepts
What is Plant Health?
It is the science that deals with the prevention and cure of plant diseases.
Disease or quarantine pest
One that may have potential or true economic importance in the area where the germplasm
is introduced; therefore it is officially controlled.
Plant quarantine
Any activity aimed at preventing the introduction and / or spread of quarantine diseases to
ensure their official control.
Quarantine rules
They are considered as a technical-legal mechanism to prevent the entry, establishment and
dissemination of quarantine plant pests and plant products. International standards for
phytosanitary measures are subject to periodic review and amendment.
8. Office of the Division of Plant Health (Section of Inspection and Quarantine) of
the Colombian Agricultural Institute (ICA), organization assigned to the
Department of Agriculture of Colombia.
ICA-CIAT Cooperation agreement: Letter of Understanding No. 9
The objectives of the program are:
to prevent the spread of seed borne diseases and to minimize the risk of
accidentally introducing exotic pests and pathogens to Colombia.
To inspect screenhouses and glasshouses where imported germplasm is
increased.
To inspect field and greenhouses where the germplasm intended for
national/international export is increased.
To test the seed health status of germplasm for conservation/distribution.
9. Germplasm Health Laboratory (GHL)
Purpose: to ensure that
the germplasm
distributed by GRP and
other CIAT projects is
free of diseases of
quarantine importance.
10.
11. GHL approving accessions
Approved accesion beans
Approved tropical grasses and legumes
2010 2011 2012 2013 Until July
2014
80% 77% 76%
84%
92%
70% 72% 70% 74% 75%
Trying to avoid exogenous
contamination.
Periodic visits to the
multiplication fields.
Collecting samples for
phytosanitary diagnostic in
the GHL.
Training field workers on
visual detection of pathogens
and disease management.
Implementation of cultural
practices (clean bags, cleaning
tools, etc.)
Implementation of best
laboratory practices.
12. 2500
2000
1500
1000
500
0
Germplasm Health Laboratory tested
2005 2006 2007 2008 2009 2010 2011 2012 2013 Until July
2014
909
572
691
1190
1602 1544
1400
776 812
2306
Other projects of CIAT
13. Developing research to improve the
health of germplasm collections
Management of the fungus complex caused by Ergot
in Brachiaria sp.
Standardization of molecular diagnostic
methodologies for virus detection in cassava.
Use of tablets in GHL operations.
14. Ergot Disease
This disease is caused by certain species
of Claviceps; it has been registered in
Brachiaria in Africa - Ethiopia, Kenya,
Malawi and Zimbabwe - in Australia and
India. In South America, this fungus has
been reported in Brazil and Colombia.
Ergot disease in the inflorescences of
Brachiaria spp. Caused by Sphacelia sp.
(asexual state of Claviceps sp.),
producing a complex composed of
various fungal quarantine fungi.
15. Cleaning of tools
Handpicking
Removing
impurities
Transportation in
cloth bags
Labeling
and sealing
Collecting in
paper bags
J.C. Ramírez, A. Ciprián, M.Cuervo, 2014
16. Pruning plot
Incineration
vegetative growth
Harvest time
Soil fungicides
Foliar fungicides
J.C. Ramírez, A. Ciprián, M.Cuervo, 2014
17. Ergot disease
No. Colony Forming Units
J.C. Ramírez, A. Ciprián, M.Cuervo, 2014
0
Accepted
Rejected
21
42
21
45
40
35
30
25
20
15
10
5
0
Primera evaluación Segunda evaluación
Number of accessions
Chemical control and cultural management
in production of Brachiaria spp
First evaluation Second evaluation
1 Evaluation 2 Evaluation
18. BENEFITS (1)
Reduced symptoms of the disease caused by Ergot
generating a decrease in the presence of fungal complex
in Brachiaria accessions.
Decrease in the inoculum source of the causative agent of
Ergot in the fields of seed production of GRP.
Increased acceptance of the phytosanitary condition of
the accessions belonging to the genus Brachiaria for
distribution and subsequent storage in the genebank.
19. Developing research to improve the
health of germplasm collections
Management of the fungus complex caused by Ergot
in Brachiaria sp.
1 2 3 4 5 6
Standardization of molecular diagnostic
methodologies for virus detection in cassava.
Use of tablets in GHL operations.
20. As in other vegetative propagated crops, our research has
revealed the common occurrence of mixed virus infections
in diseased cassava plants therefore the methodologies of
diagnosis had to be changed accordingly.
Photo by Camilo Oliveros
22. The standardization and implementation of new
molecular diagnostic techniques for cassava virus
Modification in the type of extraction, was changed to make
dsRNA extractions of total RNA extractions
Amount of RNA used
(1.8-4 μg) RNA ng/μl
Total RNA
used for cDNA
≤ 100 a 300 18μl
301 a 500 10μl
500 ≤ 5μl
1 2 3 4 5 6
LINE
CONCENTRATION
ng/μ 260/280 260/230
1 319.2 2.07 2.32
2 425.5 2.08 2.37
3 576.9 2.08 2.39
4 363.7 2.07 2.33
5 509.9 2.08 2.39
6 345.0 2.07 2.30
Amount of cDNA used
Dilution 1/10 Dilution 1/100 Dilution 1/1000 (+) (-) Bl
23. Modification in the use of primers for the realization of the
cDNA
cDNA prepared with
primer F110
(reovirus); PCR with
primer specific for
Reovirus
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 cDNA prepared with
Random-primer; PCR
with primer specific
for Reovirus
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
24. Viruses have high mutation and recombination rates. Therefore diagnostic
MCOL2737-4
MCOL2737-37
MCOL2737-56
MCOL2737-33
SM3375-113
MCOL2737-35
HEL-4
MCOL2737-36
MCOL2737-36
CM546010
FSD23
FSD80
FSD5
60
94 50
CM5460-10
Outgroup -ToTV
82
52
66
98
0.2
2014
(Eastern plains)
1980
(North Coast)
FSD5B
FSD80B
178 MCOL1754
003 MON
109 AMZ9 2
Cauca1A
17 FSD 29
111 AMZ9
16 FSD 29
181 COL1692
Llano1A
Llano1B
75
13 AMZ 16
Amz16A
69 AMZ16
AMZ 9
126 FSD29/Sec
92 FSD29
115 AMZ9 2
130 FSD29/Sec
FSD29A
FSD29B
Outgroup-RRSV
100
97
100
83
87
85
0.1
1980
(North Coast)
1990
(Amazonas
And Cauca)
Torrado
Reovirus 2005
(Eastern plains)
Jimenez, Cuervo, Carvajal, Lozano, Cuellar, 2014
methodologies must be adjusted accordingly.
This requires continued collection of virus isolates and its biological
characterization.
The method, besides providing a quick detection, also makes possible the
study of sequence variability/virus evolution.
25. Progress in certification of the CIAT collection of Manihot
Accessions available (negative for all viruses) 2233
Unavailable accessions evaluated (with positive results for at least one of
218
the viruses)
Pending accessions to assess 4220
Virus
Positive
accessions %
Cassava frogsking virus (CSFV), Reovirus: 99 4.43
Cassava polero-like virus’ (CsPLV, LUTEO) 87 3.89
Cassava Torrado-like virus’ (CsTLV) 22 0.98
Cassava new alphaflexivirus’ (CsNAV, POTEX) 16 0.71
Cassava X Virus (CsXV) 9 0.40
Cassava common mosaic virus (CCMV) 6 0.26
Other viruses evaluated
Accessions
evaluated
Negative
accessions
African cassava mosaic virus (ACMV) 382 382
Cassava Vein Mosaic virus (CVMV) 1260 1260
Virus Accessions
Cassava frogsking virus (CsFSaV) 81
Cassava polero-like virus’ (CsPLV) 67
Cassava Torrado-like virus’ (CsTLV) 19
Cassava new alphaflexivirus’ (CsNAV) 14
Cassava X virus (CsXV) 6
Cassava common mosaic virus (CsCMV) 5
CsPLV + CsFSaV 15
CsPLV + CsTLV 2
CsPLV + CsFSaV + CsTLV 1
CsPLV + CsNAV 2
CsXV + CsFSaV 2
CsCMV + CsXV 1
The low percentage of mixed infections as compared to single infections, suggests again that Secundina is
useful detecting mixed infections.
The high number of single infections indicates a dramatic improvement in indexing by using RT-PCR as
compared to Secundina.
The low porcentage of CsXV and CsCMV suggest that ELISA was efficient in detection.
M.Cuervo, A.Martinez, E. Aranzales, J.C. Ramirez,, Virology Team
26. The standardization and implementation of new
molecular diagnostic techniques for cassava virus
In cassava plants affected by the frogskin disease and the common
mosaic disease, several viruses were identified that may contribute to the
development of the disease, it is very important to make the diagnosis for
all of them.
Looking to the future, we must have the implementation of molecular
methodologies for all viruses of cassava, as in the case of CsCMV and
CsXV where the antisera are not applied anymore.
Other pathogens that are reported infecting the crop must be included.
We have to evaluate other indicator plants to replace Secundina.
27. BENEFITS (2)
With this method we can detect up to 7 viruses from one single plant RNA
extraction which saves time and money.
More sensitive and specific it is also recommended in the evaluation of virus
cleaning methods.
The method also makes possible the detection of these viruses even in
asymptomatic infections/ before they are part of a disease complex.
The molecular methods are more effective and reliable than the
symptomatology and the use of warning plants.
It can make a diagnosis earlier without waiting for the plants to grow.
There is neither leaks nor confusion for other environmental effects.
28. Developing research to improve the
health of germplasm collections
Management of the fungus complex caused by Ergot
in Brachiaria sp.
Standardization of molecular diagnostic
methodologies for virus detection in cassava.
Use of tablets in GHL operations.
29. Process before tablet Implementation
Receipt of beans and tropical forages seeds in GHL
Generate and print formats of materials to test
Fungi test Virus test Bacteria test
Register results of evaluations
Compiling and transcribing data to
electronic format
Sync data to
GRP database
A.Hernandez, D.F.Gonzalez2014
30. Process after tablet Implementation
Receipt of beans and tropical forages seeds
in Germplasm Health Laboratory
Web site
Central
Data Base
Web services
Synchronization
of active
WI FI
Access point
Upload data
of materials
to be
evaluated
Registry of evaluations
Fungi Test Bacteria Test Virus Test evaluations
A.Hernandez, D.F.Gonzalez2014
31. Benefits (3)
Material traceability through the
identification of materials with barcode.
Minimize errors in the data taking during the
evaluation.
Optimizing data collection.
Streamlining the migration of the results to
the database.
Reduction in time of giving results.
Reduction of steps doing the evaluations
(17 vs. 10 steps).
Generating easy reports.
32. Perspectives
We have very high standards to evaluate germplasmfor distribution; it
is a must.
To strengthen the CIAT diagnostic system related to the international
rules (ISTA) and standards that improve the quality and efficiency of
the laboratory.
To collaborate permanently with CIAT scientists and other
laboratories.
To improve and maintain communication with ICA officers to facilitate
the import and export of CIAT germplasm material.
To implement a program of biosecurity in the GHL.
To manage that the GHL starts to be part of the ICA national diagnostic
laboratories network (resolution 003823 of 04 September, 2013); so
the results of the lab analyses will be official.
We go to a certified laboratory !
33. Julio Cesar Ramirez
Angelica Maria Martinez
Angela Hernandez
Luis Guillermo Santos
Ericson Aranzales
Dr. Daniel Debouck
Josefina Martinez
Isabel Natalia Salas
Camilo Oliveros-Comunicaciones
Team of GHL
Cassava virology team at CIAT:
Dr. Wilmer Cuellar
Dr. Monica Carvajal
Ivan Lozano
Jenyfer Jimenez
Ana Maria Leiva