There are three main sources of genetic variability in sexually reproducing organisms: 1) Independent assortment of homologous chromosomes during metaphase I of meiosis results in random combinations of maternal and paternal chromosomes in gametes. 2) Crossing over between non-sister chromatids during prophase I shuffles genetic material. 3) Fertilization is random, with each egg being fertilized by a randomly selected sperm cell. Together, these processes generate a vast number of potential genetic combinations.