Gene electroporation, often simply referred to as electroporation, is a potent molecular biology technique employed to introduce genetic material, like plasmid DNA, RNA, or other molecules, into specific cells. This procedure leverages the transient permeabilization of the cell membrane induced by brief electrical pulses, facilitating the entry of external DNA or RNA into the cell. Typically, cells are cultivated and primed for electroporation, either in suspension or adherent culture. Subsequently, the genetic material is combined with the target cells, and in some instances, cells are positioned within a cuvette or electroporation chamber along with the genetic material.