Energy is the ability to do work.
Everything that happens in the world uses energy!
Most of the time we can’t see energy, but it is
everywhere around us!
Diabetes is a condition when a person has high blood glucose (blood sugar), either because insulin production is inadequate, or because the body's cells do not respond properly to insulin, or both.
Energy is the ability to do work.
Everything that happens in the world uses energy!
Most of the time we can’t see energy, but it is
everywhere around us!
Diabetes is a condition when a person has high blood glucose (blood sugar), either because insulin production is inadequate, or because the body's cells do not respond properly to insulin, or both.
1. Define Work
2. Express work in proper units
3. Calculate work done in simple case
4. Define Kinetic Energy
5. Express kinetic Energy in proper units
6. Solve Simple problems based on Kinetic Energy
7. Define Potential Energy
8. Define Gravitational Potential Energy
9. Solve Simple problems based on Gravitational Potential Energy
9. Describe Energy Transformation in daily life
10. Define Power
11. Distinguish between Energy and Power
1. Concept of WORK
2. Concept of ENERGY
3. Different forms of energy
Mechanical (Potential & Kinetic), Heat, Light, Chemical, Atomic, Electrical, Magnetic etc
4. Detailed idea of Mechanical Energy i.e Potential and Kinetic Energy.
5. Transformation between POTENTIAL and KINETIC energy.
6. Conservation of MECHANICAL ENERGY
7. Transformation of different ENERGIES.
8. Dissipation of ENERGY
1. Define Work
2. Express work in proper units
3. Calculate work done in simple case
4. Define Kinetic Energy
5. Express kinetic Energy in proper units
6. Solve Simple problems based on Kinetic Energy
7. Define Potential Energy
8. Define Gravitational Potential Energy
9. Solve Simple problems based on Gravitational Potential Energy
9. Describe Energy Transformation in daily life
10. Define Power
11. Distinguish between Energy and Power
1. Concept of WORK
2. Concept of ENERGY
3. Different forms of energy
Mechanical (Potential & Kinetic), Heat, Light, Chemical, Atomic, Electrical, Magnetic etc
4. Detailed idea of Mechanical Energy i.e Potential and Kinetic Energy.
5. Transformation between POTENTIAL and KINETIC energy.
6. Conservation of MECHANICAL ENERGY
7. Transformation of different ENERGIES.
8. Dissipation of ENERGY
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Nutrition is the science that deals with the study of nutrients and their role in maintaining human health and well-being. It encompasses the various processes involved in the intake, absorption, and utilization of essential nutrients, such as carbohydrates, proteins, fats, vitamins, minerals, and water, by the human body.
Gliese 12 b, a temperate Earth-sized planet at 12 parsecs discovered with TES...Sérgio Sacani
We report on the discovery of Gliese 12 b, the nearest transiting temperate, Earth-sized planet found to date. Gliese 12 is a
bright (V = 12.6 mag, K = 7.8 mag) metal-poor M4V star only 12.162 ± 0.005 pc away from the Solar system with one of the
lowest stellar activity levels known for M-dwarfs. A planet candidate was detected by TESS based on only 3 transits in sectors
42, 43, and 57, with an ambiguity in the orbital period due to observational gaps. We performed follow-up transit observations
with CHEOPS and ground-based photometry with MINERVA-Australis, SPECULOOS, and Purple Mountain Observatory,
as well as further TESS observations in sector 70. We statistically validate Gliese 12 b as a planet with an orbital period of
12.76144 ± 0.00006 d and a radius of 1.0 ± 0.1 R⊕, resulting in an equilibrium temperature of ∼315 K. Gliese 12 b has excellent
future prospects for precise mass measurement, which may inform how planetary internal structure is affected by the stellar
compositional environment. Gliese 12 b also represents one of the best targets to study whether Earth-like planets orbiting cool
stars can retain their atmospheres, a crucial step to advance our understanding of habitability on Earth and across the galaxy.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Gliese 12 b: A Temperate Earth-sized Planet at 12 pc Ideal for Atmospheric Tr...Sérgio Sacani
Recent discoveries of Earth-sized planets transiting nearby M dwarfs have made it possible to characterize the
atmospheres of terrestrial planets via follow-up spectroscopic observations. However, the number of such planets
receiving low insolation is still small, limiting our ability to understand the diversity of the atmospheric
composition and climates of temperate terrestrial planets. We report the discovery of an Earth-sized planet
transiting the nearby (12 pc) inactive M3.0 dwarf Gliese 12 (TOI-6251) with an orbital period (Porb) of 12.76 days.
The planet, Gliese 12 b, was initially identified as a candidate with an ambiguous Porb from TESS data. We
confirmed the transit signal and Porb using ground-based photometry with MuSCAT2 and MuSCAT3, and
validated the planetary nature of the signal using high-resolution images from Gemini/NIRI and Keck/NIRC2 as
well as radial velocity (RV) measurements from the InfraRed Doppler instrument on the Subaru 8.2 m telescope
and from CARMENES on the CAHA 3.5 m telescope. X-ray observations with XMM-Newton showed the host
star is inactive, with an X-ray-to-bolometric luminosity ratio of log 5.7 L L X bol » - . Joint analysis of the light
curves and RV measurements revealed that Gliese 12 b has a radius of 0.96 ± 0.05 R⊕,a3σ mass upper limit of
3.9 M⊕, and an equilibrium temperature of 315 ± 6 K assuming zero albedo. The transmission spectroscopy metric
(TSM) value of Gliese 12 b is close to the TSM values of the TRAPPIST-1 planets, adding Gliese 12 b to the small
list of potentially terrestrial, temperate planets amenable to atmospheric characterization with JWST.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
2. What is Energy?
• Energy is the ability to do work
• Everything that happens in the world uses energy!
• Most of the time we can’t see energy, but it is everywhere around us!
3. Energy….
• Is NEVER created or destroyed!
• Can only be STORED or TRANSFERRED.
4. Clip example pictures
• Batteries stored energy
• Car uses lots of energy
• Even sleeping puppy is using stored energy
• We get our energy from FOOD
15. Kinetic Energy is….
• The energy of a moving object.
• “Kinetic” means movement.
• When stored energy is being used up, it is making things move or
happen.
16.
17. • The faster an object moves,
the more Kinetic Energy is
produced.
• The greater the mass and
speed of an object, the more
kinetic energy there will be.
18. When these objects moves at the same
speed, which will have more Kinetic Energy?
20. • An object has the MOST kinetic energy when it’s movement is the
GREATEST!
• When an object has the least potential energy, it has the MOST
kinetic energy!
21.
22.
23. Sample Problems:
1.Calculate the kinetic energy of a 1000-kg car travelling at 60km/hr?
2.If a 0.2-kg ball is thrown with a velocity of 6m/s, what is its KE?
3.What is the potential energy of a 0.3-kg ball lifted to a height of 5.0m
above the ground?
4.A 1.5-kg book on top a shelf has a PE of 29.4 J. How high is the shelf?
24. A water bottle is knocked
of a desk. When does the
bottle have the MOST
kinetic energy?
25. C. At the bottom of the fall.
• It has the most kinetic energy when its movement and speed are
greatest, which is at the bottom of the fall right before it hits the
ground.
• When an object has the LEAST potential energy is when it has the
MOST kinetic energy.
26. Roller Coasters
• When does the train on this roller coaster have the
MOST potential energy?
• AT THE VERY TOP!
• The HIGHER the train is lifted by the motor, the
MORE potential energy is produced.
• At the top of the hill the train has a huge amount of
potential energy, but it has a very little kinetic
energy.
27. • As the train accelerates down the
hill the potential energy is
converted into kinetic energy.
• There is very little potential energy
at the bottom of the hill, but there
is a great amount of kinetic
energy.
28. • When does the train on this roller
coaster have the MOST kinetic
energy? (when it is moving the
fastest)
• When does it have the LEAST
potential energy? (at the bottom
of the tallest hill)
29. Notes….
• All energy is divided into two types: potential and kinetic.
• Potential Energy: The energy stored in an object.
• Kinetic Energy: The energy of a moving object.
• Energy is never created or destroyed. It is always stored or
transferred.