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INTRODUCTION
• Due to the impending energy crisis,
affordable and abundant alternative energy
sources are needed. Current forms of
renewable energy, such as solar and wind
energy, can be very expensive to install
and require upkeep.
• Algae has been studied as an important
component of bioenergy production
because of its high productivity, high oil
yield, and ability to grow in wastewater.
• In anaerobic digesters in municipal
wastewater treatment facilities all over the
country, sewage sludge is being broken
down into methane and carbon dioxide. By
adding a new ingredient into the anaerobic
digester with the sewage, the methane
yield can increase and be used as a form
of energy.
PREDICTED RESULTS
• The Modified Bold’s Basal Medium should
continue to grow filamentous algae from
wastewater samples.
• TSS, VSS, COD, TS, and VS values should
decrease overtime as the septic tank sewage
breaks down.
• Based on previous research, sewage and
algae mixtures made up of 11% and 41%
algae should produce the greatest gas yields
and improve the solids reduction and
dewatering rate.
Creating Energy from our Waste
Michaela Fitzgerald, Dingnan Lu, Professor Xiaoqi Zhang
Department of Civil & Environmental Engineering
1 Wang, W., Sahu, A. K., Rusten, B., Park, C., 2013. Anaerobic co-digestion of microalgae Chlorella sp. and waste activated sludge. Bioresource Technology, 142, 585-590.
2Yen, H., Brune, D. E., 2007. Anaerobic co-digestion of algal sludge and waste paper to produce methane. Bioresource Technology. 98(1), 130-134.
3"What Is Anaerobic Digestion?" Renewable Energy Alternatives ,LLC - Digester Basics. N.p., n.d. Web. 29 July 2014.
APPROACH
• In our experiment, we will grow cultivate
algae using samples obtained from the
Lowell Wastewater Treatment Plant
(WWTP). Then, we will pre-treat the algae
samples and add them to the anaerobic
digester with 5L of sewage sludge from the
Lowell WWTP.
• Over the course of 60 days, we will monitor
methane gas production and try to
determine an optimum algae-to-sewage
ratio.
DATAPROCEDURE:
Algae samples were collected from the primary and secondary clarifier
tanks at the Lowell WWTP. These samples were combined with a media
known as Modified Bold’s Basal Medium in a 500 mL Erlenmeyer Flask.
The samples were supplied with light and sparged with air for 14 days.
After analyzing the algae samples under a microscope, we determined
that we grew primarily 2 species of filamentous algae known as
spirogyra and oedogonium. Since chlorella could not compete with these
large algae species, we believed that there was no chlorella in our
samples. However, we decided to continue with our experiment using
our cultivated algae samples.
Since most algae have strong cell walls they tend to digest very slowly.
A pre-treatment method known as the Cambi Process will be used to
speed up the process. The algae samples will be heated at 165˚C for
30 minutes.
In this step, the treated algae will be combined with 5L of septic tank
sludge in an anaerobic digester. It will be continuously mixed for 60
days. Several tests, such as total suspended solids (TSS), volatile
suspended solids (VSS), total and soluble chemical oxygen demand
(COD), total solids (TS), and volatile solids (vs) will be done daily. pH is
also monitored daily and adjusted to around 7 if needed.
The process of anaerobic digestion will produce biogas. Over the course
of the 60 day digestion process, several gas samples will be collected
from gas bags connected to the digester and analyzed by a gas
chromatograph. The volume and percentage of methane (CH4) and
carbon dioxide (CO2) will be recorded.
14 days into Algae Cultivation
The Anaerobic Digester with
5L septic tank sewage
SIMILAR RESEARCH
•The University of Massachusetts Amherst:
cultivated Chlorella sp. from local WWTP and
combined it with varying percentages of waste
activated sludge also from the local WWTP.
Results showed that co-digesting Chlorella with
the sludge increased biogas yield by 70%.1
•Tunghai University, Taiwan: the goal of their
experiment was to produce high levels of
methane by co-digesting algal sludge and high
carbon content waste paper. They recognized
that anaerobic digestion is directly affected by
the Carbon to Nitrogen (C/N) ratio. At 50%
waste paper and 50% sludge, the C/N ratio
was 20:1 and the methane production peaked
at 1170 ml/ 1 day.2
The Control Module for the
Anaerobic Digester
3
Anaerobic
Digestion
Process
Day 1 Day 2 Day 3 Day 4 Day 5
pH (am) 6.89 6.78 6.9 6.78 6.98
pH (pm) 6.3 6.16 6.33 6.16 6.5
COD
(Total
mg/L)
6350 6300 6150 5500 5610
COD
(Soluble
mg/L)
990 960 900 890 855
TS
(mg/L)
5990 5870 5830 5740 5610
VS
(mg/L)
5260 5230 5190 5200 5120
TSS
(mg/L)
3900 3750 3500 3538 3160
VSS
(mg/L)
3300 3140 3050 2938 2790
Anaerobic Digestion of Septic-tank Sludge
The digester is Bioflo 110, New Brunswick Scientific
Co., with 5 L working volume under 37ºC
consistently stirred at 50 rpm.
0
1000
2000
3000
4000
5000
6000
7000
1 2 3 4 5
mg/L
Parameters of Anaerobic Digestion of
Septic-tank Sludge
COD (Total mg/L) COD (Soluble mg/L)
TS (mg/L) VS (mg/L)
TSS (mg/L) VSS (mg/L)
Day
Key:

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Fitzgerald_poster

  • 1. INTRODUCTION • Due to the impending energy crisis, affordable and abundant alternative energy sources are needed. Current forms of renewable energy, such as solar and wind energy, can be very expensive to install and require upkeep. • Algae has been studied as an important component of bioenergy production because of its high productivity, high oil yield, and ability to grow in wastewater. • In anaerobic digesters in municipal wastewater treatment facilities all over the country, sewage sludge is being broken down into methane and carbon dioxide. By adding a new ingredient into the anaerobic digester with the sewage, the methane yield can increase and be used as a form of energy. PREDICTED RESULTS • The Modified Bold’s Basal Medium should continue to grow filamentous algae from wastewater samples. • TSS, VSS, COD, TS, and VS values should decrease overtime as the septic tank sewage breaks down. • Based on previous research, sewage and algae mixtures made up of 11% and 41% algae should produce the greatest gas yields and improve the solids reduction and dewatering rate. Creating Energy from our Waste Michaela Fitzgerald, Dingnan Lu, Professor Xiaoqi Zhang Department of Civil & Environmental Engineering 1 Wang, W., Sahu, A. K., Rusten, B., Park, C., 2013. Anaerobic co-digestion of microalgae Chlorella sp. and waste activated sludge. Bioresource Technology, 142, 585-590. 2Yen, H., Brune, D. E., 2007. Anaerobic co-digestion of algal sludge and waste paper to produce methane. Bioresource Technology. 98(1), 130-134. 3"What Is Anaerobic Digestion?" Renewable Energy Alternatives ,LLC - Digester Basics. N.p., n.d. Web. 29 July 2014. APPROACH • In our experiment, we will grow cultivate algae using samples obtained from the Lowell Wastewater Treatment Plant (WWTP). Then, we will pre-treat the algae samples and add them to the anaerobic digester with 5L of sewage sludge from the Lowell WWTP. • Over the course of 60 days, we will monitor methane gas production and try to determine an optimum algae-to-sewage ratio. DATAPROCEDURE: Algae samples were collected from the primary and secondary clarifier tanks at the Lowell WWTP. These samples were combined with a media known as Modified Bold’s Basal Medium in a 500 mL Erlenmeyer Flask. The samples were supplied with light and sparged with air for 14 days. After analyzing the algae samples under a microscope, we determined that we grew primarily 2 species of filamentous algae known as spirogyra and oedogonium. Since chlorella could not compete with these large algae species, we believed that there was no chlorella in our samples. However, we decided to continue with our experiment using our cultivated algae samples. Since most algae have strong cell walls they tend to digest very slowly. A pre-treatment method known as the Cambi Process will be used to speed up the process. The algae samples will be heated at 165˚C for 30 minutes. In this step, the treated algae will be combined with 5L of septic tank sludge in an anaerobic digester. It will be continuously mixed for 60 days. Several tests, such as total suspended solids (TSS), volatile suspended solids (VSS), total and soluble chemical oxygen demand (COD), total solids (TS), and volatile solids (vs) will be done daily. pH is also monitored daily and adjusted to around 7 if needed. The process of anaerobic digestion will produce biogas. Over the course of the 60 day digestion process, several gas samples will be collected from gas bags connected to the digester and analyzed by a gas chromatograph. The volume and percentage of methane (CH4) and carbon dioxide (CO2) will be recorded. 14 days into Algae Cultivation The Anaerobic Digester with 5L septic tank sewage SIMILAR RESEARCH •The University of Massachusetts Amherst: cultivated Chlorella sp. from local WWTP and combined it with varying percentages of waste activated sludge also from the local WWTP. Results showed that co-digesting Chlorella with the sludge increased biogas yield by 70%.1 •Tunghai University, Taiwan: the goal of their experiment was to produce high levels of methane by co-digesting algal sludge and high carbon content waste paper. They recognized that anaerobic digestion is directly affected by the Carbon to Nitrogen (C/N) ratio. At 50% waste paper and 50% sludge, the C/N ratio was 20:1 and the methane production peaked at 1170 ml/ 1 day.2 The Control Module for the Anaerobic Digester 3 Anaerobic Digestion Process Day 1 Day 2 Day 3 Day 4 Day 5 pH (am) 6.89 6.78 6.9 6.78 6.98 pH (pm) 6.3 6.16 6.33 6.16 6.5 COD (Total mg/L) 6350 6300 6150 5500 5610 COD (Soluble mg/L) 990 960 900 890 855 TS (mg/L) 5990 5870 5830 5740 5610 VS (mg/L) 5260 5230 5190 5200 5120 TSS (mg/L) 3900 3750 3500 3538 3160 VSS (mg/L) 3300 3140 3050 2938 2790 Anaerobic Digestion of Septic-tank Sludge The digester is Bioflo 110, New Brunswick Scientific Co., with 5 L working volume under 37ºC consistently stirred at 50 rpm. 0 1000 2000 3000 4000 5000 6000 7000 1 2 3 4 5 mg/L Parameters of Anaerobic Digestion of Septic-tank Sludge COD (Total mg/L) COD (Soluble mg/L) TS (mg/L) VS (mg/L) TSS (mg/L) VSS (mg/L) Day Key: