This document discusses file handling in C++. It begins by explaining the differences between main memory and secondary memory (files on storage devices). It then discusses C++ streams and the classes used for file input/output (ifstream, ofstream, fstream). The rest of the document covers various file operations like opening, closing, reading from and writing to files. It also discusses text files versus binary files and sequential versus random file access. File pointers and associated functions like seekg(), tellg(), seekp() and tellp() are explained for navigating within files. An example program demonstrates reading from one file and writing to another.
File Handling is used in C language for store a data permanently in computer.
Using file handling you can store your data in Hard disk.
http://www.tutorial4us.com/cprogramming/c-file-handling
Python too supports file handling and allows users to handle files i.e., to read and write files, along with many other file handling options, to operate on files. The concept of file handling has stretched over various other languages, but the implementation is either complicated or lengthy, but alike other concepts of Python, this concept here is also easy and short. Python treats file differently as text or binary and this is important. Each line of code includes a sequence of characters and they form text file. Each line of a file is terminated with a special character, called the EOL or End of Line characters like comma {,} or newline character. It ends the current line and tells the interpreter a new one has begun. Let’s start with Reading and Writing files.
This was the fifth and last presentation in pySIG, 2015 @ BMS College of Engineering, Bangalore. The code and assignments can be found at https://github.com/pranavsb
h2kinfosys is offering the IT Online Courses with Certificates ,H2kinfosys is the best place to learn online coding classes as we offer the most job oriented training led by experienced instructors through live classroom sessions. It courses online from h2kinfosys . top trending courses like learn tableau online, hadoop certification Training, python certification online and more courses register for free demo class .
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This is ppt prsented by me in class in this ppt i include file handling in which i tell us about the types of files
creation of text file in C ++ ,updating a text file in C++ , printing the inforamtion in text file .creation of binary file in C ++ ,updating a binary file in C++ , printing the inforamtion in binary file, text file function and binary file function in c++ , File ponters ,syntax of every thing and use of file pointers and many more.
File Handling is used in C language for store a data permanently in computer.
Using file handling you can store your data in Hard disk.
http://www.tutorial4us.com/cprogramming/c-file-handling
Python too supports file handling and allows users to handle files i.e., to read and write files, along with many other file handling options, to operate on files. The concept of file handling has stretched over various other languages, but the implementation is either complicated or lengthy, but alike other concepts of Python, this concept here is also easy and short. Python treats file differently as text or binary and this is important. Each line of code includes a sequence of characters and they form text file. Each line of a file is terminated with a special character, called the EOL or End of Line characters like comma {,} or newline character. It ends the current line and tells the interpreter a new one has begun. Let’s start with Reading and Writing files.
This was the fifth and last presentation in pySIG, 2015 @ BMS College of Engineering, Bangalore. The code and assignments can be found at https://github.com/pranavsb
h2kinfosys is offering the IT Online Courses with Certificates ,H2kinfosys is the best place to learn online coding classes as we offer the most job oriented training led by experienced instructors through live classroom sessions. It courses online from h2kinfosys . top trending courses like learn tableau online, hadoop certification Training, python certification online and more courses register for free demo class .
https://www.h2kinfosys.com/
This is ppt prsented by me in class in this ppt i include file handling in which i tell us about the types of files
creation of text file in C ++ ,updating a text file in C++ , printing the inforamtion in text file .creation of binary file in C ++ ,updating a binary file in C++ , printing the inforamtion in binary file, text file function and binary file function in c++ , File ponters ,syntax of every thing and use of file pointers and many more.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
2. Files (Streams)
Files are used to store data in a relatively
permanent form, on floppy disk, hard disk,
tape or other form of secondary storage.
Files can hold huge amounts of data if need
be. Ordinary variables (even records and
arrays) are kept in main memory which is
temporary and rather limited in size. The
following is a comparison of the two types
of storage:
3. Main memory
Made up of RAM chips.
Used to hold a program
when it is running,
including the values of its
variables (whether
integer, char, an array,
etc.)
Can only hold relatively
small amounts of data.
Is temporary (as soon as
the program is done or the
power goes out all of
these values are gone).
Gives fast access to the
data (all electronic).
Secondary memory
Usually a disk drive (or magnetic
tape).
Used to hold files (where a file
can contain data, a program, text,
etc.)
Can hold rather large amounts of
data.
Is fairly permanent. (A file
remains even if the power goes
out. It will last until you erase it,
as long as the disk isn't damaged,
at least.)
• Access to the data is
considerably slower (due to
moving parts).
4. C++ STREAMS
A Stream is a general name given to flow of
data.
Different streams are used to represent
different kinds of data flow.
Each stream is associated with a particular
class, which contains member functions and
definitions for dealing with that particular
kind of data flow.
5. Flow of Data….
PROGRAM
DEVICES OR
FILES
Input
Stream
>>
Output
Stream
<<
Data
Data
istream class ostream class
(Insertion
operator)
(Extraction
operator)
6. The following classes in C++
have access to file input and
output functions:
ifstream
ofstream
fstream
7. The Stream Class Hierarchy
ios
istream
get()
getline()
read()
>>
ostream
put()
write()
<<
fstreambase
iostream
Ifstream
Open()
Tellg()
Seekg()
Ofstream
Open()
Tellp()
Seekp()
fstream
NOTE : UPWARD ARROWS INDICATE
THE BASE CLASS
9. OPENING A FILE
1. By using the CONSTRUCTOR of the
stream class.
ifstream transaction(“sales.dly”);
ofstream result(“result.02”);
2. By using the open() function of the stream
class
ifstream transaction;
transaction.open(“sales.dly”);
(Associating a stream with a file)
10. File Mode Parameters
PARAMETER MEANING
Ios::app Append to end-of file
Ios::ate goto end of file on opening
Ios::binary binary file
Ios::in Open existing file for reading
Ios::nocreate open fails if file doesn’t exist
Ios::noreplace open fails if file already exists
Ios::out creates new file for writing on
Ios::trunc Deletes contents if it exists
The mode can combine two or more modes using bit wise
or ( | )
11. Checking For Successful File Opening
ifstream transaction(“sales.dly”);
if (transcation == NULL)
{
cout<<“unable to open sales.dly”;
cin.get(); // waits for the operator to press any key
exit(1);
}
13. Types of Files
. The two basic types are
– text and
– binary.
A text file consists of readable characters
separated into lines by newline characters.
(On most PCs, the newline character is
actually represented by the two-character
sequence of carriage return (ASCII 13), line
feed (ASCII 10).
14. A binary file stores data to disk in the same
form in which it is represented in main
memory.
If you ever try to edit a binary file containing
numbers you will see that the numbers
appear as nonsense characters. Not having to
translate numbers into a readable form makes
binary files somewhat more efficient.
Binary files also do not normally use
anything to separate the data into lines. Such
a file is just a stream of data with nothing in
particular to separate components.
15. When using a binary file we write whole
record data to the file at once. When using a
text file, we write out separately each of the
pieces of data about a given record.
The text file will be readable by an editor,
but the numbers in the binary file will not
be readable in this way.
The programs to create the data files will
differ in how they open the file and in how
they write to the file.
16. For the binary file we will use write to
write to the file, whereas for the text file we
will use the usual output operator(<<) and
will output each of the pieces of the record
separately.
With the binary file we will use the read
function to read a whole record, but with
the text file we will read each of the pieces
of record from the file separately, using the
usual input operator(>>)
18. :
Sequential access. With this type of file
access one must read the data in order,
much like with a tape, whether the data is
really stored on tape or not.
Random access (or direct access). This
type of file access lets you jump to any
location in the file, then to any other, etc.,
all in a reasonable amount of time.
Types of File Access
20. FILE POINTERS
Each file object has two integer values
associated with it :
– get pointer
– put pointer
These values specify the byte number in the
file where reading or writing will take
place.
21. File pointers…..
By default reading pointer is set at the
beginning and writing pointer is set at the
end (when you open file in ios::app mode)
There are times when you must take control
of the file pointers yourself so that you can
read from and write to an arbitrary location
in the file.
22. Functions associated with file
pointers :
The seekg() and tellg() functions allow you
to set and examine the get pointer.
The seekp() and tellp() functions allow you
to set and examine the put pointer.
23. seekg() function :
With one argument :
seekg(k) where k is absolute position from
the beginning. The start of the file is byte 0
Begin File End
k bytes ^
File pointer
The seekg() function with one argument
24. seekg() function :
With two arguments :
the first argument represents an offset from a particular
location in the file.
the second specifies the location from which the offset is
measured.
Begin End
^
Offset from Begin
The seekg() function with two argument
25. seekg() function :
With two arguments :
Begin End
^
Offset from Begin
The seekg() function with two argument
^
^
Offset from end
Offset from current
position
26. //
#include <fstream.h>
#include <conio.h>
#include <stdio.h>
void main()
{
//clrscr();
char c,d,ans;
char str[80];
ofstream outfl("try.txt"),out("cod.dat");
ifstream infl;
do
{ cout<<"please give the string : ";
gets(str);
outfl<<str;
cout <<"do you want to write more...<y/n> : ";
ans=getch();
}
while(ans=='y');
outfl<<'0';
outfl.close();
//clrscr();
getch();
cout <<"reading from created file n";
infl.open("try.txt");
out.open("cod.dat");
//**********************************
c=infl.get();
do
{ d=c+1;
cout<<c<<d<<'n';
out.put(d);
c= infl.get();
}
while (c!='0');
out<<'0';
infl.close();
outfl.close();
getch();
//*********************************
}