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Objectives :

   a) To analyse the kidney’s function in maintaining acid-base balance.

   b) To identify the certain normal constituents of urine.

   c) To study the principle of Multistix and Weyl Test.

Materials and Methods : Refer to Laboratory Manual, Universiti Tunku Abdul
                            Rahman page 63-64
Results :

   1) Ammonium

                     Tube contains                              Observation
                        Urine                         Clear solution and pungent smell
                                                            evolved after boiling.
                           NH3                        Clear solution and pungent smell
                                                            evolved after boling.


   2) Creatinine (Weyl test)

                   Test                                  Observation
                                               Urine                   Creatinine
            Sodium Nitroprusside      Color changed to darker     Color changed to light
                                     yellow from light yellow.     red from colorless.
             Sodium Hydroxide          Color changed to light      Colored changed to
                                              orange.                  darker red.
            Glacial Acetic Acid        Color changed to light    Colored changed to light
                                              yellow.                    yellow.




   3) Uric Acid

                    Test                                 Observation
                                              Urine                   Sodium Urate
              Folin Reagent-           Color changed from          Color changed from
            Phosphotungstic acid     yellow to dark blue. The     colorless to dark blue.
                                           pH is 7 or 8.              The pH is 12.




   4) Glucose (Benedict’s Test)

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Expt 3

  • 1. Objectives : a) To analyse the kidney’s function in maintaining acid-base balance. b) To identify the certain normal constituents of urine. c) To study the principle of Multistix and Weyl Test. Materials and Methods : Refer to Laboratory Manual, Universiti Tunku Abdul Rahman page 63-64 Results : 1) Ammonium Tube contains Observation Urine Clear solution and pungent smell evolved after boiling. NH3 Clear solution and pungent smell evolved after boling. 2) Creatinine (Weyl test) Test Observation Urine Creatinine Sodium Nitroprusside Color changed to darker Color changed to light yellow from light yellow. red from colorless. Sodium Hydroxide Color changed to light Colored changed to orange. darker red. Glacial Acetic Acid Color changed to light Colored changed to light yellow. yellow. 3) Uric Acid Test Observation Urine Sodium Urate Folin Reagent- Color changed from Color changed from Phosphotungstic acid yellow to dark blue. The colorless to dark blue. pH is 7 or 8. The pH is 12. 4) Glucose (Benedict’s Test)