The document outlines an experiment with the following objectives: 1) To analyze the kidney's role in maintaining acid-base balance. 2) To identify normal urine constituents. 3) To study the principle of Multistix and Weyl tests. The methods are referred to in a laboratory manual. Results show urine samples changing color when reacted with various reagents, indicating the presence of ammonium, creatinine, uric acid, and glucose.

1. Objectives :
a) To analyse the kidney’s function in maintaining acid-base balance.
b) To identify the certain normal constituents of urine.
c) To study the principle of Multistix and Weyl Test.
Materials and Methods : Refer to Laboratory Manual, Universiti Tunku Abdul
Rahman page 63-64
Results :
1) Ammonium
Tube contains Observation
Urine Clear solution and pungent smell
evolved after boiling.
NH3 Clear solution and pungent smell
evolved after boling.
2) Creatinine (Weyl test)
Test Observation
Urine Creatinine
Sodium Nitroprusside Color changed to darker Color changed to light
yellow from light yellow. red from colorless.
Sodium Hydroxide Color changed to light Colored changed to
orange. darker red.
Glacial Acetic Acid Color changed to light Colored changed to light
yellow. yellow.
3) Uric Acid
Test Observation
Urine Sodium Urate
Folin Reagent- Color changed from Color changed from
Phosphotungstic acid yellow to dark blue. The colorless to dark blue.
pH is 7 or 8. The pH is 12.
4) Glucose (Benedict’s Test)