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Explaining "pathology" in digital pathology
- 1. Explaining the “pathology” in “digitalpathology” Yves Sucaet December 2023 www.cytomine.com
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- 3. If want themake the best tools…
- 4. … understand wheredata comes from
- 5. Internal questions andcuriosity
- 6. Where does thedata come from? When papa data and mama data love each other very much… www.cytomine.com
- 7. From patient to(stained) slide(s)
- 8. Step 1: tissueharvesting (surgeon)
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- 11. Step 2: slidepreparation
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- 18. How does amicrotome work? • https://www.youtube.com/watch?v=KnMdSgd5mts
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- 21. Step 2: Differenttypes of artifacts
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- 27. How does a(Dako) autostainer work? • https://www.youtube.com/watch?v=OkUfldg6-CA
- 28. Step 3: protocolselection www.stainsfile.com
- 29. Step 3: Additionaltypes of artifacts
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- 31. If all wentwell, you have histology! • Histology = study of normal tissue • Pathology = study of diseased tissue • H & E = Hematoxylin & Eosin stain (chemicals)
- 32. If all wentwell, you have histology! • Histology = study of normal tissue • Pathology = study of diseased tissue • H & E = Hematoxylin & Eosin stain (chemicals)
- 33. If all wentwell, you have histology! • Histology = study of normal tissue • Pathology = study of diseased tissue • H & E = Hematoxylin & Eosin stain (chemicals)
- 34. If all wentwell, you have histology! • Histology = study of normal tissue • Pathology = study of diseased tissue • H & E = Hematoxylin & Eosin stain (chemicals)
- 35. If all wentwell, you have histology! • Histology = study of normal tissue • Pathology = study of diseased tissue • H & E = Hematoxylin & Eosin stain (chemicals)
- 36. What does theslide scanner do? • It automates the microscope!
- 37. What does theslide scanner do? • It automates the microscope!
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- 41. What does thepathologist see? What do YOU see? What organ? Healthy or sick?
- 42. What does thepathologist see? Healthy lung tissue; note the blood and sacks of air Diseased lung tissue; NSCLC; tight cells; bloated nuclei
- 43. Ask for additionalstains as needed
- 44. Ask for additionalstains as needed
- 46. What does thepathologist do?
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- 49. Join me nexttime! For more technical information on the “digital” in “digital pathology” www.cytomine.com