Digital pathology is a recent hybrid field. It involves expertise from computer science and medicine alike. This presentation targets ICT staff and software development that wants to understand more of the background on where whole slide images originate from and what unique challenges this represents.

1. Explaining the “pathology”
in
“digital pathology”
Yves Sucaet
December 2023
www.cytomine.com

2. Why this presentation?
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3. If want the make the best tools…

4. … understand where data comes from

5. Internal questions and curiosity

6. Where does the data come from?
When papa data and mama data love each other very much…
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7. From patient to (stained) slide(s)

8. Step 1: tissue harvesting (surgeon)

9. Step 1: theory

10. Step 1: practice

11. Step 2: slide preparation

12. Step 2: theory

13. Step 2: practice

14. Step 2: practice

15. Step 2: practice

16. Step 2: practice

17. Step 2: practice

18. How does a microtome work?
• https://www.youtube.com/watch?v=KnMdSgd5mts

19. Step 2: practice

20. Step 2: practice

21. Step 2: Different types of artifacts

22. Step 3: staining

23. Step 3: theory

24. Step 3: practice

25. Step 3: practice

26. Step 3: automation

27. How does a (Dako) autostainer work?
• https://www.youtube.com/watch?v=OkUfldg6-CA

28. Step 3: protocol selection www.stainsfile.com

29. Step 3: Additional types of artifacts

30. Histology and pathology
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31. If all went well, you have histology!
• Histology = study of normal tissue
• Pathology = study of diseased tissue
• H & E = Hematoxylin & Eosin stain (chemicals)

32. If all went well, you have histology!
• Histology = study of normal tissue
• Pathology = study of diseased tissue
• H & E = Hematoxylin & Eosin stain (chemicals)

33. If all went well, you have histology!
• Histology = study of normal tissue
• Pathology = study of diseased tissue
• H & E = Hematoxylin & Eosin stain (chemicals)

34. If all went well, you have histology!
• Histology = study of normal tissue
• Pathology = study of diseased tissue
• H & E = Hematoxylin & Eosin stain (chemicals)

35. If all went well, you have histology!
• Histology = study of normal tissue
• Pathology = study of diseased tissue
• H & E = Hematoxylin & Eosin stain (chemicals)

36. What does the slide scanner do?
• It automates the microscope!

37. What does the slide scanner do?
• It automates the microscope!

38. Histology 101

39. Histology 102

40. Histology 102

41. What does the pathologist see?
What do YOU see? What organ? Healthy or sick?

42. What does the pathologist see?
Healthy lung tissue; note the blood and sacks of air Diseased lung tissue; NSCLC; tight cells; bloated
nuclei

43. Ask for additional stains as needed

44. Ask for additional stains as needed

46. What does the pathologist do?

47. Pathology evaluation

48. The goal

49. Join me next time!
For more technical information on the “digital” in “digital pathology”
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