Titulo Ponencia: Endophytes Identification: morphological methods Autor: Dr. Gary Strobel Evento TF Innova: Workshop Biotechnology "Isolation and identification of endophytic fungi from vascular plants"

1. Montana State University

2. Pestalotiopsis in the World’s Rainforests

4. Pestalotiopsis microspora an Endophytic Fungus
1. One of the most frequently observed
endophytes.
2. Found in all rainforests and most plant
species.
3. It has hundreds of synonyms.
4. Exists as many biotypes and makes hundreds
of natural products.
5. Some biotypes are easily genetically
transformed.

5. Pestalotiopsis microspora (culture and
spores)

6. The Acervulus and Conidia of
Pestalotiopsis microspora

7. Pestalosphaeria hansenii on Barley

8. The Perithecium and Ascopsores of
Pestalosphaeria hansensii, Perfect Stage of
Pestalotiopsis microspora

11. Picking the Spines of Cholla Cactus
To Support the Bioassay Chemicals

12. Developing a Bioassay for Sex
Inducing Chemicals found in Plants

14. Ability of Various Fractions of T cuspidata to
Induce the formation of fruitng structures

15. The Induced Perithecia of Pestalosphaeria

18. Protoplasts of P. microspora Ready
for Genetic Transformation

19. Selection of Hygromycin Resistant Isolates

24. Methylene chloride extracts
of P. microspora isolates from
around the world

25. Sepik River Area of Papua New Guinea

26. Sepik River Area of Papua New Guinea

27. Crystals of Pestacin

28. Pestacin-a
Powerful
Antioxidant

29. Isopestacin- an
antioxidant

30. Sampling trees for Endophytic
Fungi in the Highlands of Papua
New Guinea

31. Pestalotiopsis jesterii, a fungal endophyte

32. Conidiospores of P. jesterii

36. Test Fungus Natural Synthetic
MIC MIC
P. Ultimum 25 25
R. Solani 25 50
G. Candidum 100 100
P. oryzae 25 25
S. sclerotiorum 100 100

37. Huli Wigmen of Papua New Guinea

38. Pestalotiopsis
microspora as
an endophytic
rainforest
fungus has
made the “big
time” as a
producer of
new natural
products

39. The Orchid -Dendrochilum sp.

40. Solid State NMR of Ambuic Acid

41. Ambuic Acid is
produced by
various isolates of
Pestalotiopsis
microspora
obtained in various
rainforest
locations around
the world

42. Torreya taxifolia and torreyanic acid

43. Torreyanic acid
Anticancer
agent

44. Pestaloside