Electrophoresis is a technique used to separate molecules like proteins and nucleic acids based on their size, charge, or other properties. It works by applying an electric field to move charged particles through a medium like agarose gel or polyacrylamide gel. There are several types of electrophoresis that differ in things like the medium used and the molecules separated. It has various applications in fields like molecular biology, biochemistry, genetics, and medicine.
Gel electrophoresis is a method for separation and analysis
of macromolecules (DNA, RNA and proteins) and
their fragments, based on their size and charge.
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge.
Different types of electrophoresis.
Gel electrophoresis; Agarose Gel electrophoresis; polyacrylamide gel electrophoresis; pulsed-field gel electrophoresis
Agarose gel electrophoresis by KK Sahu sirKAUSHAL SAHU
INTRODUCTION.
HISTORY.
PROCESS OF GEL ELECTROPHORESIS.
AGAROSE GEL ELECTROFORESIS.
POLYACRYALAMIDE GEL ELECTRIPHORESIS.
GEL CONDITION.
DENATURETION.
NATIVE.
BUFFERS.
USES.
CONCLUSION.
REFFERENCES.
Gel electrophoresis is a method for separation and analysis
of macromolecules (DNA, RNA and proteins) and
their fragments, based on their size and charge.
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge.
Different types of electrophoresis.
Gel electrophoresis; Agarose Gel electrophoresis; polyacrylamide gel electrophoresis; pulsed-field gel electrophoresis
Agarose gel electrophoresis by KK Sahu sirKAUSHAL SAHU
INTRODUCTION.
HISTORY.
PROCESS OF GEL ELECTROPHORESIS.
AGAROSE GEL ELECTROFORESIS.
POLYACRYALAMIDE GEL ELECTRIPHORESIS.
GEL CONDITION.
DENATURETION.
NATIVE.
BUFFERS.
USES.
CONCLUSION.
REFFERENCES.
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
General introduction about electrophoresis
Principle
Working condition of electrophoresis
Factors affecting separation of electrophoresis
Application of electrophoresis
Types of electrophoresis
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is used to move molecules to be separated through a gel. Pores in the gel work like a sieve, allowing smaller molecules to move faster than larger molecules.
Gel Electrophoresis, ITS FACTOR AFFECTING, ITS TYPES,NORMAL METHODOLOGY, PAGERitamMukherjee11
ELECTROPHORESIS may be defined as the migration of the charged particle through a solution under the influence of an external electric field.
GEL ELECTROPHORESIS is a technique used to separate biomacromolecules (such as DNA, RNA, proteins, etc.) and their fragments based on their size and charge, by applying an electric field to a gel with small pores.
INTRODUCTION - Separation is brought about through molecular sieving techniques, based on the molecular size of the substances. Gel material acts as a “ molecular sieve”
It is important that support media is electrically neutral.
There are different types of gel that can be used, they are Agarose, Polyacrylamide, Starch, and Sephadex.
A porous gel acts as a sieve by retarding or, in some cases by completely obstructing the movement of macromolecules which allowed smaller molecules to migrate freely.
PRINCIPLE - According to charge: When charged molecules are placed in an electric field, they migrate toward either the positive (anode) or negative (cathode) pole according to their charge.
According to size: The smaller molecules move more swiftly than the larger-sized ones, as they can travel through the pores more easily than the later.
According to Molecular weight: The smaller molecular weight will move faster than the larger molecular weight compound.
Running of Agarose Gel Electrophoresis Practical Sabahat Ali
Electrophoresis technique used for separation of Macromolecules(DNA, Proteins & their derivatives)
Separation occur on the basis of charge to size ratio
it is a short ppt. on Electrophoresis. Which gives it's defination, Types of electrophoresis ; Like:- Zone electrophoresis, Isoelectric focussing, Immunoelectrophoresis. and their images.
General introduction about electrophoresis
Principle
Working condition of electrophoresis
Factors affecting separation of electrophoresis
Application of electrophoresis
Types of electrophoresis
What is Electrophoresis?
Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
This electrokinetic phenomenon was first observed in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate.
Electrophoresis of positively charged particles (cations) is called cataphoresis while electrophoresis of negatively charged particles (anions) is called anaphoresis.
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is used to move molecules to be separated through a gel. Pores in the gel work like a sieve, allowing smaller molecules to move faster than larger molecules.
Gel Electrophoresis, ITS FACTOR AFFECTING, ITS TYPES,NORMAL METHODOLOGY, PAGERitamMukherjee11
ELECTROPHORESIS may be defined as the migration of the charged particle through a solution under the influence of an external electric field.
GEL ELECTROPHORESIS is a technique used to separate biomacromolecules (such as DNA, RNA, proteins, etc.) and their fragments based on their size and charge, by applying an electric field to a gel with small pores.
INTRODUCTION - Separation is brought about through molecular sieving techniques, based on the molecular size of the substances. Gel material acts as a “ molecular sieve”
It is important that support media is electrically neutral.
There are different types of gel that can be used, they are Agarose, Polyacrylamide, Starch, and Sephadex.
A porous gel acts as a sieve by retarding or, in some cases by completely obstructing the movement of macromolecules which allowed smaller molecules to migrate freely.
PRINCIPLE - According to charge: When charged molecules are placed in an electric field, they migrate toward either the positive (anode) or negative (cathode) pole according to their charge.
According to size: The smaller molecules move more swiftly than the larger-sized ones, as they can travel through the pores more easily than the later.
According to Molecular weight: The smaller molecular weight will move faster than the larger molecular weight compound.
Running of Agarose Gel Electrophoresis Practical Sabahat Ali
Electrophoresis technique used for separation of Macromolecules(DNA, Proteins & their derivatives)
Separation occur on the basis of charge to size ratio
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
ELECTROPHORESIS.pptx
1.
2. WHAT IS ELECTROPHORESIS?
Electrophoresis is motion of dispersed particles
relative to a fluid under the influence of a spatially
uniform electric field.
This electro kinetic phenomenon was observed for the
first time in 1807 by Reuss.
There are several types of electrophoresis but the
concept is same.
3. TYPES OF ELECTROPHORESIS
1. HIGH RESOLUTION ELECTROPHORESIS
2. AGAROSE GEL ELECTROPHORESIS
3. POLYACRYLAMIDE GEL ELECTROPHORESIS
4. ISOELECTRIC FOCCUSING
5. ROUTINE ELECTROPHORESIS
6. CAPILLARY ELECTROPHORESIS
7. TWO DIMENSIONAL ELECTROPHORESIS
8. IMMUNOCHEMICAL ELECTROPHORESIS
IMMUNOFIXATION ELECTROPHORESIS
ELECTROIMMUNOASSAY
9. PULSED FIELD ELECTROPHORESIS
4. GENERAL PRINCIPLE
Electrophoresis is a technique used to separate
and sometimes purify macromolecules -
especially proteins and nucleic acids - that differ
in size, charge or conformation
When charged molecules are placed in an
electric field, they migrate toward either the
positive or negative pole according to their
charge.
In contrast to proteins, which can have either a
net positive or net negative charge, nucleic acids
have a consistent negative charge imparted by
their phosphate backbone, and migrate toward
the anode.
5. ROUTINE ELECTROPHORESIS
Routine electrophoresis is a generic term for the
traditional clinical laboratory electrophoresis which is
performed on a rectangle shaped slab of gel.
It is mostly used for separation of protiens and has some
use in separating nucleic acids.
Generally several patient specimen and control can be
placed on one gel and solute separated in one run.
This electrophoresis is sometimes called as zone
electrophoresis.
6. GEL ELECTROPHORESIS
ELECTRO= flow of electricity
PHORESIS= to carry across
• A gel is a colloid, a suspension of tiny particles in a
medium, occurring in a solid form, like gelatin.
• This refers to separation of charged particles located in
a gel when an electric current is applied.
• Charged particles include DNA, RNA, amino acids,
peptides etc.
8. STEPS
1.PREPARE AGAROSE GEL
2. ADD ETHIDIUM BROMIDE
3.POUR INTO CASTING TRAY
4. PUT GEL IN PLASTIC TRAY & ADD BUFFER
5. SAMPLES ARE PLACED IN GEL
6. START ELECTROPHORESIS
7.AFTER ELECTROPHORESIS IS COMPLETE READ UNDER
UV LIGHT
10. 1
Plasmid
Vector
DNA
2
2
1
Add DNA Sample onto
agarose gel lane 2
(DNA ladder is in lane 1)
3
NEGATIVE
ELECTRIC
CURRENT
POSITIVE
DNA bands
Are
separated
by size
4
Dye
added
binds to
DNA
5 DNA bands are
exposed on film
Under uv light
DNA is
visualized
1KB
500 bp
200 bp
GEL ELECTROPHORESIS FOR DNA
SEPARATION
11. POLYACRYLAMIDE GEL
ELECTROPHORESIS
SDS-PAGE ( sodium dodecyl sulfate polyacrylamide gel
electrophoresis)
widely used in biochemistry, molecular, forensic and
genetics to separate proteins according to their
electrophoretic mobility( a function of length/
molecular weight)
separates protein only on basis of molecular weight
and no other property.
16. STAINING
COOMASSIE BRILLIANT BLUE MOST COMMONLY USED
DESTAINED WITH METHANOL/ACETIC
ACID.
20-60 MINUTES
SILVER STAINING IF THE STAIN IS LIGHT.
DEVELOP WITH SODIUM SULPHATE/
FORMALDEHYDE
17. 1
PROTEIN
2
- - - - - - - -
ADD SDS
SDS binds to amino acid residue
And gives uniform negative charge
To protein. With heat protein is
Linearized.
3
Add protein sample on SDS PAGE
gel lane 2
1 2
4
NEGATIVE ELECTRIC
CURRENT
POSITIVE
Protein bands
Are separated by
size
PROTEIN ELECTROPHORESIS METHOD
18. ADVANTAGES
Polyacrylamide forms gels with pores of a much more controlled and
uniform size than does agarose.
Consequently, polyacrylamide gels can be used to separate molecules
that differ in size by as little as 2% of their molecular weight.
The range of pore sizes possible is also much broader.
Acrylamide is particularly superior when very small pore sizes are
needed. It can be used to effectively separate small molecules (i.e.
DNA oligonucleotides of 100 bases or less)
Another advantage of acrylamide as a gel matrix is that it is much
stronger than agarose, producing gels that do not tear as nearly as
easily.
It is also possible to load larger quantities of material onto
acrylamide gels and to recover the material in a very pure form.
19. DISADVANTAGES
Polyacrylamide gels are difficult to prepare when
compared with agarose gels and the risk associated with
their preparation is much greater.
It is most dangerous in its powder form, when it is easily
inhaled.
Masks, gloves and safety glasses must always be worn
when working with polyacrylamide powder.
20. USES
SDS PAGE:-
MOLECULAR BIOLOGY
BIOCHEMISTRY
GENETIC STUDY
FORENSIC
MICROBIOLOGY
GEL ELECTROPHORESIS:-
MASS SPECTROMETRY
RFLP
PCR
CLONING
DNA SEQUENCING
SOUTHERN BLOT
21. ELECTROPHORESIS FOR
SICKLE CELL ANAEMIA
Pattern of hemoglobin electrophoresis from several different individuals.
Lanes 1 and 5 are hemoglobin standards. Lane 2 is a normal adult.
Lane 3 is a normal neonate. Lane 4 is a homozygous HbS individual.
Lanes 6 and 8 are heterozygous sickle individuals.
Lane 7 is a SC disease individual.
22. ELECTROPHORESIS FOR
MULTIPLE MYELOMA
The normal plasma protein electrophoretic pattern shows a heavy albumin band
(to the left) and lighter bands which are alpha, beta and gamma mobility
globulins. The serum from the patient with multiple myeloma shows a heavy
band which is the abnormal monoclonal immunoglobulin (referred to as a
paraprotein). Because it is monoclonal (all molecules identical) it is a very
narrow discrete band, which in this patient has gamma mobility.
α β γ