Downstream Processing

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Code No: R05412304
Set No. 1
IV B.Tech I Semester Regular Examinations, November 2008
DOWNSTREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Describe about economic assessment of various proteins via r-DNA. [16]

2. (a) What are the parameters used in characterization in fermentation broth?
(b) List down all the important impurities and containments present in DSP and
their removal techniques. [16]
3. Write short notes on the following.

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(a) Enzymatic cell lysis
(b) Chemical cell lysis

(c) Concentration polarization and cross ow ltration [16]

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4. Write short notes on :

(a) Liquid membranes
(b) Ultra ltration. [16]

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5. (a) Write about the criteria used for selection of extraction equipment in antibiotic
industry.
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(b) A compound X is to be extracted from a clari ed fermentation beer by using
pure amyl acetate as solvent at pH 4.0. The distribution coe cient K of the
system was found to be 30. The initial concentration of compound x in the
feed is 400ml/L. The ow rates of the feed and solvent streams are 500L/H
t
and 30L/hr respectively. How many ideal stages are required to recover 90%
i.cen

of X in the feed. [8+8]

6. Write the mode of separation of compounds by capillary electrophoresis. Discuss
the mode of operation by a schematic diagram. [16]
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7. Write short notes on:

(a) Peak asymmetry
(b) Selectivity factor
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(c) Stationary phase
(d) Gradient elution. [4×4=16]
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8. Discuss the pro cess of crystallization. What are the di erent parameters considered
before crystalliaing a compound?
[16]
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Code No: R05412304
Set No. 1

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Code No: R05412304
Set No. 2
(Bio-Technology)

1. What is the role of pro cess engineer in Bioseparation process? Explain in detail
with help of suitable examples. [16]

2. What are the ma jor steps involved in the product isolation and puri cation of citric
acid manufacturer? [16]

3. (a) Discuss the theoretical principles of constant pressure ltration.

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(b) How is compressibility of a cake determined? [16]

4. Explain in detail how do you classify the membrane separation methods according
to particle size. [16]

5. What is integrated bioprocessing. Draw a neat schematic diagram for a known

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compound of your interest . What are the likely problems encountered in the
pro cess and the necessary steps to overcome them to upkeep the process e ciency.
[16]

of an unknown protein on the gel.
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6. Write the procedure of SDS-PAGE. Discuss how to calculate the molecular weight
[16]

7. (a) Write the principle of Gel chromatographu.
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(b) What is the retention volume (VR) if external solution (V0) is 16ml and inter-
nal solution is 5 ml and fraction of (Vi ) acceptable to solute is 12ml in a gel
chromatography column? [16]

8. (a) Write about the process of crystal formation and the geometrical changes
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associated with it
(b) Write the signi cance of crystallization in pro duct recovery. [8+8]
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Code No: R05412304
Set No. 3
(Bio-Technology)

1. Discuss about the classical and modern biotechnology consideration of downstream
pro cessing in Biotech industry. [16]

2. Discuss the steps involved the product isolation and puri cation of an antibiotic
pro duction. [16]

3. Write notes on the operation of tubular bowl centrifuge and disc stack bowl cen-

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trifuge.
[16]

4. (a) Discuss about the classi cation of membrane separation process.
(b) What are the transport models for membrane process? Explain about them.

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[16]

5. Explain “in situ product removal as a tool for bioprocessing”. [16]

6. (a) Describe about di erent types of support media employed in electrophoresis.
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(b) How to prepare the sample of a nucleic acid to load on an Agarose gel. [16]

7. (a) Write about di erent types of Stationary phases available for Gas chromatog-
raphy.
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(b) What is the percentage composition of the mixture of Ethane, Propane, butane
if in Gas chromatography separation the peak aeas were 53.2, 14.5 and 31 cm.
[16]

8. Write the principle and process of Crystallization. Discuss how it is di erent from
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precipitation.
[16]
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Code No: R05412304
Set No. 4
(Bio-Technology)

1. Give generalized pro cess recovery scheme for Enzyme from plant source with help
of solid state fermentation. [16]

2. Write in detail about the selecting of various unit operations in relation to their
separation factor and the molecular size of the material to be recovered in DSP.
[16]

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3. (a) Explain the principle of centrifugal separation.
(b) What is a gyro tester? What are its uses? [16]

4. (a) What are the advantages of membrane separation pro cess?
(b) What is Ultra ltration? How is it useful in bio separation? [16]

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5. What is in situ product removal in bioprocessing. What are the disadvantages of
using ISPR and the necessary precautions in ISPR operation. [16]

6. Short notes on

(a) Capillary array electrophoresis
(b) Isoelectric focusing
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(c) Capillary zone electrophoresis
(d) Electro osmotic ow. [4×4]

7. Write short notes on:

(a) Temperature programming in GC
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(b) Band separation

(c) Electron capture detector
(d) Open tubular columns. [4×4]
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8. What is crystallization and how it is common and di erent from lyophilisation.
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[16]
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Downstream Processing

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