22301 Analytical Techniques In Bio Technology Set1

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Code No: RAR05322301
RA
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
1. Explain the following terms associated with Fluorescent Microscope.
(a) Barrier lter
(b) Heat lter
(c) Fluorochromes
(d) Exciter lter. [4+4+4+4]
2. What is the di erence between a TEM and a SEM? [16]
3. How is blo od sugar measured? [16]
4. What are the factors a ecting electrophoretic mobility of any molecule? [16]
5. Explain the basic principle behind all forms of Chromatography [16]
6. How is PCR used to sequence DNA? [16]
7. Write short notes on
(a) Rocket Immunoelectroforesis
(b) Radial Immunoassays.
[8+8]
8. Explain
(a) Substrate uptake in absence of product formation.
(b) Substrate uptake with product formation. [8+8]
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