Proposed d ch-e_ pg-BIOCHEMICAL ENGG

NATIONAL INSTITUTE OF TECHNOLOGY
DEPARTMENT OF CHEMICAL ENGINEERING
TIRUCHIRAPPALLI, 620 015

PROPOSAL FOR CONDUCTING NEW
M.TECH. DEGREEP COURSE IN

BIOCHEMICAL ENGINEERING

2004 - 2005

(JANUARY, 2004)
SUBMITTED TO
MINISTRY OF HUMAN RESOURCE DEVELOPMENT
DEPARTMENT OF EDUCATION
GOVERNMENT OF INDIA
NEW DELHI- 110 001

3.8 ACTIVITY : INTRODUCTION OF NEW COURSES UNDER TEQIP-2003

PROPOSAL FOR STARTING A NEW P.G. COURSE IN
EMERGING AREA OF BIOCHEMICAL ENGINEERING
AT THE DEPARTMENT OF CHEMICAL ENGGINEERING, N.I.T.T

Proposed By: Dept. of Chemical Engineering

Justification for the course

a) Biochemical engineering is the process engineering component of biotechnology
and is an emerging area of importance .It contributes significantly to many
economic activities of our country such as agricultural productivity, production of
biologicals, antibiotics, bioenergy and environmental protection through
biological waste water treatment and bioremediation of contaminated solids or
land. Training of engineers in this interdisciplinary area would benefit many fields
of human endeavor. There is a good demand for highly qualified and trained
engineers in this field.

Additional
Level Proposed
Name of the Year of Technical
Justification U.G. / annual
program starting staff
P.G. intake
required
M. Tech Emerging Prof:1 ,A.P:
(Chemical Area of 2
P.G. 25 2004
Engineering) biotechnology Lect.:4
Technical 4

Table ggg

Name of the Total Details
Name of the
S.No Department / central requirements
laboratory
faculty (Rs. Lakhs) Annexure no Page No
1 Bioscience
Chemical
2 Bioprocess 1
Engineering
3 CRE

Chemical Engineering - Biochemical Engineering 2

ANNEXURE -1

DEPARTMENT OF CHEMICAL ENGINEERING - M. Tech- Biochemical Eng.
Equipments Required in the Bioscience and Bio Process Laboratories
Name of the Present quantity
Name of the Unit Price Quantity
S.No equipment with brief available in the
Laboratory (Rs.in Lakhs) required
specification Institution
1 Bio science Spectro colorimeter 0.50 1 N/A
Bioprocess Electronic digital 3.75 5 N/A
2
balance
3 Air oven 0.50 2 N/A
4 Muffle furnace 0.50 2 N/A
5 Soxhlet‟s appartus 0.15 N/A
6 Turbidometer 0.15 1 N/A
7 Flame photometer 1.0 1 N/A
Constant temperature 0.25 2 2
8
bath
9 Ultra centrifuge 5.00 N/A
11 COD apparatus 0.10 1 N/A
12 BOD incubator 0.50 1 N/A
13 Ultrasonicator 0.10 1 N/A
Microfiltration 4.00 1 N/A
14
Membrane device
15 Densitometer 2.00 1 N/A
16 Vortex mixer 0.20 1 N/A
17 Vacuum oven 0.30 1 N/A
18 Air curtains 0.10 10 N/A
19 Blender 0.20 1 N/A
20 Homogenizer 3.00 1 1
21 Colony counter 0.10 1 N/A
22 De humidifier 0.20 2 N/A
23 Polarimeter 0.5 2 1
Water and soil 0.5 1 N/A
24
analyser
25 Vacuum pumps 0.15 3 1
26 Glasswares 1.00
27 Chemicals 2.00
28 Deep Freezer 1.00 1
Total 27.75


List of equipments coating above Rs. 5 lakhs

Present Utilization
Name of the Unit
quantity in
Name of the equipment with price Quantity
S.No available hours/week
Department brief (Rs. required
in the of present
specification lakhs)
institution equipment
1 Chemical UV- visible 20.00 1 N/A
Engg. spectrophotometer
with CRT screen
2 Inoculation 10.00 1 N/A
chamber
3 Autoclave 5.00 N/A
4 Ion 15.00 1 N/A
chromatography
5 Differential 25.00 1 N/A
Scanning
Calorimeter
(Fourier Analysis)
6 Digital 5 1 N/A
Polarimeter
7 Four- plate Hot 6.5 3 N/A
plate / Strirrer
8 Fat Determination 8 1 N/A
System (Non
gravimetric)
9 Modular 7.5 1 N/A
Rheometer
10 Near IR Liquid 6 1 N/A
Fuel Analyser
11 Thin layer 10.00 1 N/A
chromatography
12 pH meter 10.0 2 N/A
Total 128

.


3.5 Modernization and Strengthening of Departmental Laboratories and
Workshops.
Project Oriented Laboratory for Bio-catalytic Reaction Engineering and
Environmental Water Pollution Control:

 Enhancing facilities for projects on Immobilized Bio-catalytic Systems and
Enzyme Engineering Studies
 Studies on industrial wastewater treatment methods including biological
wastewater treatment.
 Design and Modeling and simulation of Bioreactors.

The modernization of Bio – catalytic Reaction Engineering Laboratory is required for
training the U.G. and P.G. students to advanced and industry oriented problems as
project works and will also enhance the R & D activity leading to Doctoral
programmes and increase research work in this area among the faculty

Present Utilization
Name of the Unit
quantity in
Name of the equipment price Quantity
S.No available hours/week
Department with brief (Rs. required
in the of present
specification lakhs)
institution equipment
1 Chemical Supercritical 20.00 1 1 N/A
Engg. fluid extraction
&
chromatography
systems
2 Lab. Scale 25.00 1 1 N/A
Fluidized bed
reactor with
internal
circulation loop
3 Inoculation 10.00 1 N/A
chamber
4 Autoclave 5.00 1 N/A
5 Ion 25.00 1 1 N/A
chromatography
6 Total Sulphur 6.00 1 1 N/A
Analyser
7 Metering 7.00 5 1 N/A
pumps
Total 98.00


Category of Items : Text and Reference Books for Bio Chemical Engineering

No of
Name of the Author Unit Cost
Title copies
(Publishers) Rs.
required
Robert H.Perry and
Perry‟s Chemical Engineer, Platinum
Don. W.Green, 2600 1
Edition, The Reference Collection
Mcgraw-Hill
Johan Stichlmair et al,
Bio Technology : Principles and
John Wiley & sons, 6296.5 1
Practice
1998
W.R.Vieth,Wiley- Membrane Systems: Analysis &
Interscience reissue Design: Applications in 5750.0 1
edition,1993 biotechnology
A.S. Grandison & M.J. Separation Process in the food and
7000 1
Lewis Biotechnology Industries
T.K.Ghose (Ed.)
Process Computations in
(1994) Tata McGraw 450 1
Biotechnology
Hill Publ.Co
Handbook of Chemical and
Joseph P. Reynolds
Environmental Engineering 750 1
(Author), et al
Calculations
David T. Allen
Green Engineering: Environmentally
(Author), David R.
Conscious Design of Chemical 2000 1
Shonnard
Processes
Scale-Up and Optimization in
Preparative Chromatography:
Anurag S. Rathore
Principles and Biopharmaceutical 2500 1
(Editor), et al (2003)
Applications (Chromatographic
Science, Vol 88)
Stuttgart
Biochemical engineering 7200 1

T. K. Ghose (Editor), Biotechnology in India (Advances in
P. Ghosh (Editor) Biochemical Engineering/
2460 2
Biotechnology), vol 84 and 85

New Trends and Development in 1300
T. H. Scheper 1
Biochemical Engineering

Relation Between Morphology and
K. Schugerl (Editor) Process Performances (Advances in
7250 1
Biochemical Engineering
/Biotechnology), Vol 60
.


Category of Items : Text and Reference Books

No of
Title copies
(Publishers) Rs.
required

Metabolic Engineering (Advances in
Jens Nielsen, et al Biochemical Engineering/ 5000 1
Biotechnology, 73)

History and Trends in Bioprocessing
and Biotransformation (Advances in
T. Scheper (Editor), et 5000 1
Biochemical Engineering /
al
Biotechnology, 75
Tools and Applications of
K. Schugerl (Editor), Biochemical Engineering Science
A. -P. Zeng (Editor) (Advances in Biochemical 4680 1
Engineering/Biotechnology, 74)

Biopolyesters. With contributions by
numerous experts (Advances in
Wolfgang Babel ,
Biochemical Engineering 8000 1
Alexander Steinbüchel
Biotechnology Vol. 71

Biotransformations (Advances in
K. Faber (Editor), T.
Biochemical E ngg /Biotechnology , 5400 1
Scheper (Editor)
Vol 63

New Products and New Areas of
T. Scheper (Editor) Bioprocess Engineering (Advances
4264 1
in Biochemical
Engineering/Biotechnology, 68

Harvey W. Blanch,
Biochemical Engineering 2502 1
Douglas S. Clark

A. Constantinides,
Biochemical Engineering II 2750 1
W.R. Vieth (Editor)
Modern Biochemical Engineering
A. Fiechter (Editor), T. (Advances in Biochemical
5000 1
Scheper Engineering/Biotechnology, Vol 46)


Category of Items : Text and Reference Books 1

No of
Title copies
(Publishers) Rs.
required
Fermentation and Biochemical
H. C. Vogel (Editor) Engineering Handbook: Principles, 3150 1
Process Design, and Equipment
Henry R. Bungay
(Editor), Georges Advanced Biochemical Engineering 1900 1
Belfort (Editor)

Total

.


M. Tech. DEGREE

(BIOCHEMICAL ENGINEERING)

SYLLABUS FOR

CREDIT BASED CURRICULUM
(24 months duration)
2004 - 2005

Department of Chemical Engineering

NATIONAL INSTITUTE OF TECHNOLOGY

TIRUCHIRAPPALLI - 620 015.


M. Tech. (Bio-chemical Engineering) COURSE CURRICULUM

Code & Name of the subject Hours per week Credits Evaluation (Marks)
Semester - 1 L T P C.A. S.E. Lab. Total

MBC 101Microbiology 3 3 50 50 100
MBC 102 Biochemistry 3 3 50 50 100
MBC 103 Biochemical Eng. 3 3 50 50 100
MBC 104 Bio reaction Eng. 3 3 50 50 100
Elective - I 3 3 50 50 100
MBC 108 Bioscience Lab 9 3 50 50 100 100
Total Credits in Semester 1 = 18
Name of the subject Hours per week Credits Evaluation (marks)
Semester - 2 L T P C.A. S.E. Lab. Total

MBC 201 Bioprocess Equip- 3 3 50 50 100
Design
MBC 202 Bioprocess - 3 3 50 50 100
Instrumen- Control
MBC 203 Bioprocess Model - 3 3 50 50 100
simulation
Elective - II 3 3 50 50 100
Elective - III 3 3 50 50 100
MBC 208 Bioprocess 9 3 50 50 100 100
Laboratory
Semesters- 3 Hours per week Credits Evaluation (marks)

Name of the subject L T P C.A. S.E. Lab. Total
Elective IV 3 3 50 50 100
Elective V 3 3 50 50 100
Elective VI 3 3 50 50 100
Project Work 6 50 50 100
Semesters -4

Project Work 12 50 50 100



Total Credits in the Course = 63

CODE NO: M. Tech: Biochemical Engineering Page Number
TITLES OF CORE SUBJECTS
MBC 101 Microbiology 4

MBC 102 Applied biochemistry 5

MBC 103 Bioprocess Engineering 6

MBC 104 Bio reaction Engineering 7

MBC108 Bioscience Laboratory 8-9

MBC 201 Bioprocess equipment Design 10

MBC 202 Bioprocess Instrumentation and Control 11

MBC 203 Bioprocess Modeling and Simulation 12

MBC 208 Bioprocess Laboratory 13

CODE NO: TITLES OF ELECTIVE SUBJECTS

MBC E 01 Separation & Analytical Techniques 14

MBC E 02 Recombinant DNA Technology 15

MBC E 03 Food Processing Technology 16

MBC E 04 Enzyme Engineering 17

MBC E 05 Plant Cell Culture 18

MBC E 06 Environmental Biotechnology 19

MBC E 07 Pharmaceutical Technology 20

MBC E 08 Applied Bio-informatics 21

MBC E 09 Animal Cell Technology 22

MBC E 10 Bioenergy 23


MBC 101 INDUSTRIAL MICROBIOLOGY

1. WORLD OF MICROORGANISMS
Characteristics of microorganisms. Historical review of the Foundation of Microbiology.
Taxonomy methods of studying microorganisms. Microscopy, Light and Laser optic systems.
General structural organisation of bacterial, viral, bacilli, Actinomycetes, Differentiation and
development.

2. REPLICATION OF MICROORGANISMS & MICROBIAL NUTRITION
Multiplication of bacteriophages, bacteria and differentiating organisms such as yeast, fungi and
actinomycetes. Growth of microorganisms in different media. Growth curve of microbes and
different methods of enumeration of multiplying microorganisms.

3. MICROBIAL METABOLISM
Metabolic pathways and bioenergetics. Aerobic and Anaerobic growth. Production of primary and
secondary metabolites and their application in Industry. Beneficial Microorganisms and products .

4. PHYSICAL & CHEMICAL CONTROL OF MICROORGANISMS
Chemotherapy -Antimicrobial agents and disinfectants - Diseases caused by microorganisms and
control.

5. ENVIRONMENTAL APPLICATIONS OF MICROBIOLOGY
Ecology - Re-cycling of Biomaterials. Production of Biogas - Leaching of ores by
microorganisms - Application of Biofertilizers and Bio pesticides - Pollution control through use
of consortium of microorganisms.

TEXT BOOKS :
1. Pelczar M.J., Chan E.C.S., Krieg, N.R. “Microbiology”, 5 th Edition, 1986,Tata McGraw-Hill
Book Co., New Delhi.

2. Schlegel H .G., General Microbiology, 7th Edition, 1994. C L P Edition, Cambridge University
Press, U.K.

3. Introductory Microbiology, J. Heritage, E. G. V. Evans and R. A. Killington, 1996, C L P Edition,
Cambridge University Press, U.K.

4. Microbiology: A text book for University Students by P.D. Sharma, Rastogi Publications
”Gangotri”, Shivaji Road, Meerut 250 002


MBC 102 APPLIED BIOCHEMISTRY

1. INTRODUCTION TO BIOMOLECULES

Structure and properties of Mono, Di, oligo and polysaccharides; Complex carbohydrates;
Structure and properties of Fatty acids; Structure and properties of amino acids, peptides, proteins
and conjugated proteins:

2. NUCLEIC ACIDS

Structure and properties of purines, pyrimidines, nucleosides, nucleotides, polynucleotides;
Ribonucleic acids, and deoxyribonucleic acids.

3. METABOLISM

Biosynthesis and degradation of fatty acids and cholesterol; amino acids and proteins; Purines,
pyrimidines and nucleic acids.

4. INTERMEDIARY METABOLISM

TCA cycle, glycolysis, gluconeogenesis, Pentose phosphate shunt; EMP, Urea cycle,
Interconnection of pathways; Metabolic regulation; Bioenergetics; Respiratory chain, ATP cycle,
energy rich compounds.

5. STRUCTURE FUNCTION RELATIONSHIP

Complex carbohydrates, proteins and nucleic acids

Text Books :
1. A. L. Lehninger, D.L.Nelson and M .M. Cox; “Principles of Biochemistry” CBS
Publications, 1993.

2. L. Stryer; “Biochemistry” W. H. Freeman (IV Ed.) 1995.

3. E .E. Conn and P.K. Stumpf and others. Outlines of Biochemistry, (V Ed.), 1987,
John Wiley & Sons, Singapore.

4. Sawhney. S. K. and Randhir Singh (Editors), Introductory Practical
Biochemistry, Narosa publishing House, New. Delhi. 2000, ”Gangotri”, Shivaji
Road, Meerut 250 002


MBC 103 BIOPROCESS ENGINEERIN

1. INTRODUCTION: FERMENTATION PROCESSES
General requirements of fermentation processes - An overview of aerobic and anaerobic
fermentation processes and their application in industry - Medium requirements for fermentation
processes - examples of simple and complex media - Design and usage of commercial media for
industrial fermentation - Sterilization :Thermal death kinetics of microorganisms - Batch and
Continuous Heat-Sterilization of liquid Media - Filter Sterilization of Liquid Media and Air.
8
Hours

2. ENZYME TECHNOLOGY, MICROBIAL METABOLISM:
Enzymes: Classification and properties-Applied enzyme catalysis - Kinetics of enzyme catalytic
reactions - Metabolic pathways - Protein synthesis in cells.
8 Hours

3. STOICHIOMETRY AND KINETICS OF SUBSTRATE UTILIZATION AND
BIOMASS AND PRODUCT FORMATION:

Stoichiometry of microbial growth, Substrate utilization and product formation-Batch
and Continuous culture, Fed batch culture

8 Hours
4. BIOREACTOR AND PRODUCT RECOVERY OPERATIONS:
Operating considerations for bioreactors for suspension and immobilised cultures, Selection,
scale-up, operation of bioreactors-Mass Transfer in heterogeneous biochemical reaction systems;
Oxygen transfer in submerged fermentation processes; oxygen uptake rates and determination of
oxygen transfer rates and coefficients; role of aeration and agitation in oxygen transfer. Heat
transfer processes in Biological systems. Recovery and purification of products.
8 Hours

5. INTRODUCTION TO INSTRUMENATION AND PROCESS CONTROL IN
BIOPROCESSES:
Measurement of physical and chemical parameters in bioreactors- Monitoring and control of
dissolved oxygen, pH, impeller speed and temperature in a stirred tank fermentor.

8 Hours
TEXT BOOKS.

1. M.L. Shuler and F. Kargi," Bio-process Engineering", Prentice Hall Inc., New Jersey. 1992.
2 J.E. Bailey and D.F. Ollis," Biochemical Engineering Fundamentals", 2nd Edn., McGraw Hill,
Publishing Co. New York., 1985.

REFERENCES:

1. P.Stanbury, A. Whitakar and S. J. Hall "Principles of Fermentation Technology" 2 nd Edn.,
Elsevier-Pergamon Press, 1995.


MBC 104
BIOCHEMICAL REACTION ENGINEERING

1. STOICHIOMETRIC OF A BIOPROCESS:

Elemental analysis and empirical formula for cells- Substrate consumption for biomass
formation, energy and product formation- Yield coefficients for biomass and product
formation- Uses of material and energy balances.

2. IDEALIZED REACTOR TYPES:

Basic types of bioreactors - Reactor circuits - Dispersion models- Reactor
Combinations - Partial Cell recycle

3. KINETICS OF CELL GROWTH AND PRODUCT FORMATION IN
IDEALIZED BIOREACTORS:

Qualitative description of growth and product formation. Growth models. Product formation.
Growth in idealized bioreactors. Non-limited and substrate limited growth. Oxygen limited
growth Model extension by death and maintenance term. Air lift reactor.

4. KINETICS OF IMMOBILIZED IOCATALYSTS AND REACTOR
CONFIGURATIONS:

Effect of external diffusion limitation in attached film particle- Effect of Internal diffusion
limitation in gels and Pores - Configurations for Immobilized Enzyme Reactors: Batch
stirred – continuous stirred tank-plug flow- packed beads- liquid-solid and three phase
expanded / fluidised bed reactors-membrane bioreactor

5. BIOREACTOR DESIGN & PRODUCT YIELD:

Strategies for Scale up for CSTR, Batch reactor, Plug flow reactor, Packed bed reactor and
fluidised bed reactor, Strategies for sterilization

Text books:

1. Karl Schugerl, “Bioreaction Engineering” Volume I, (1985), John
Wiley, New York.

2. Biochemical Reaction Engineering, R. Lovitt and M. Jones, Chapter 5 in
Chemical Engineering Vol 3, (third Edition), Richardson and Peacock,
1994, Asian Books (pvt) Ltd, N. Delhi.

3. Scragg, “Bioreactors in Biotechnology - A Practical Approach” (1991), Ellis
Horwood Ltd., U.K.


MBC 108 BIOSCIENCE LABORATORY

PART A

1. Centrifugation: Differential and gradient centrifugation. Isolation of sub-
cellular organelles and particles

2. Chromatography: Use of paper chromatography in separation of amino
acids.
Gel filtration chromatography, Gas-liquid chromatography

3. Adsorption & Ion-exchange: Separation of amino acids by ion exchange
chromatography, Separation of leaf pigments by adsorption chromatography

4. Electrophoresis in Agarose and SDS gels:

5. Membrane separation of Proteins

6. Estimation of Carbohydrates

7. Estimation of Vitamins

Reference books:

1. Plummer. D. T., “An introduction to Practical Biochemistry” 3rd Edn, (1987),
Tata McGraw Hill Publishing Co, N. Delhi.

2. Jayaraman.J, Laboratory Manual in Biochemistry, (1992), New Age
International (P), Ltd., New Delhi.

3. Sawhney. S. K. and Randhir Singh (Editors), Introductory Practical
Biochemistry, Narosa publishing House, New. Delhi. 2000, ”Gangotri”,
Shivaji Road, Meerut 250 002


MBC 108 BIOSCIENCE LABORATORY

PART B

1. Maintenance and Identification of microorganisms

2. Biochemical Characterisation.

3. Methods of quantification of microorganisms from soil, air and water.

4. Fermentation: Growth curve in Shake flask and Bioreactors,

Reference Books :
1.Collins and Lyne, “Microbiological Methods” 5th Edn.
(1986), Butterworths, Singapore
2.Laboratory Manual in Microbiology. P. Gunasekaran
(1995), New Age International (P) Ltd., N. Delhi.
3. Microbiology:A textbook for University Students, P. D. Sharma, Rastogi
Publications,


MBC 201 BIOPROCESS EQUIPMENT DESIGN
Faculty Member: Dr. P. Sivashanmugam

OBJECTIVE: The course will equip the students to design bioprocess systems

1. MECHANICAL DESIGN OF BIOREACTORS:

Basic design and construction of a fermenter and its ancillaries, Vessel geometry. Materials of
construction.

2. MECHANICAL DESIGN OF ANCILLARY EQUIPMENT:

Bearing assemblies, Motor Drives; Aseptic Seals; Flow Measuring devices; Agitator and Sparger
design; Piping and Valves for Biotechnology Plants.

3. SUPPORT AND UTILITY SYSTEMS:

Pumps; Cartridge filtration and Pressure Relief; Sterilization of process equipment; Utilities for
biotechnology Plants: Heating, Ventilating and Air Conditioning.

4. PROCESS DESIGN AND OPERATION OF BIOREACTORS:

Operational Modes of Reactors - Batch, Continuous, Fed-batch, Repetitive Batch, Recycle and
Continuous cultivation; Novel Bioreactors; Stirred Tank, Air lift and Loop reactors, Packed bed
and Hollow fibre Membrane Bioreactors; Scale-up criteria for bioreactors.

5. BIOPROCESS ECONOMICS:

Bio-product regulation; General fermentation process economics; Examples - Fine chemicals, Bulk
oxygenates, Single-cell protein, Anaerobic Methane production.

TEXT BOOKS :
1. Bioprocess Engineering: Systems Equipment and Facilities, Edited by B.K.Lederson, N.A.D‟elia
and K.L.Nelson (1994), John Wiley and Sons, N.Y.
2. Process Equipment Series, No5: Transfer Operations in Process Industries- Design and Equipment,
Edited by M .V. Bhatia, (1994), Technomic Publ. Co., Lancaster, Pennsylvania, USA.

REFERENCES:
1. Atkinson, B. & Mavituna, F. “Biochemical Engineering & Biotechnology Handbook”, (2 nd Edn.),
1993, McGraw Hill, N.York.
2. H.J. Rehm and G, Reed (ED.) Biotechnology-. Volume 3, (2nd Ed), 1993, VCH, Berlin.
3. McNeil and Harvey, “Fermentation - A Practical Approach” (1990), IRL Press, U.K.
4. Scragg, “Bioreactors in Biotechnology - A Practical Approach” (1991). Ellis Horwood Ltd , U.K.


MBC 202
BIOPROCESS INSTRUMENTATION & CONTROL

1. SENSORS:

Physical and chemical sensors; Biosensors; On-line sensors for cell properties; off-line Analytical
methods.

2. MEASUREMENT OF FERMENTATION PARAMETERS:

Agitation and capacity coefficient in fermenters; Control of pH, dissolved
oxygen, dissolved carbon dioxide, temperature of fermenters; Rheological
measurement and control application of microcomputers in the study of
microbial process.

3. COMPUTERS AND INTERFACES:

Elements of Digital computers; Computer Interfaces and peripheral devices; Fermentation
software systems

4. DATA ANALYSIS & PROCESS CONTROL:

Data smoothing and interpolation; State and parameter estimation; Direct regulatory control;
cascade control of metabolism.

5. ADVANCED CONTROL STRATEGIES:

Programmed batch bio-rection; Design and operation strategies for batch plants; Continuous
process control.

TEXT BOOKS:

1. Bailey J.E. and Ollis,D.F. “ Biochemical Engineering Fundamentals” 2 nd Edition, (1986),
McGraw Hill Book CO.,Singapore.
2. T.K.Ghose (Ed.) “Process Computations in Biotechnology” (1994) ,Tata McGraw Hill
Publ.Co.,N.Delhi.
3. A.Fischer (Ed.), “Advances in Bio Chemical Engineering ” Vol. 13, 1973,
Springer Verlag, Germany

REFERENCES:
1. Aiba, Humphry and Millis, “ Bio Chemical Engineering ”, 2 nd Ed., (1973), Academic press
2. McNeil and Harvey, “Fermentation - A Practical Approach” (1990). IRL Press , U.K.
3. Scragg, “Bioreactors in Biotechnology - A Practical Approach” (1991),Ellis Horwood Ltd.,U.K.


MBC 203
MODELLING AND SIMULATION OF BIOPROCESSES

1. MODELING PRINCIPLES
Basic modelling principles; Mathematical models for chemical engineering systems. Analog
simulation; Digital simulation; Time-domain dynamics; Laplace-domain dynamics; Frequency-
domain dynamics; Process identification.

2. MODELLING AND DESIGN OF BIORECATORS
Modelling of Non-Ideal Behaviour in Bioreactors - Tanks -in-series and Dispersion Models -
application to design of Continuous sterilizers and other first order processes; Design and analysis
of packed bed and membrane biorectors; Recombinant Cell culture processes.

3. MONITORING OF BIOPROCESSES
On-line data analysis for measurement of important physico-chemical and biochemical parameters;
State and parameter estimation techniques for biochemical processes.

4. SIMULATION OF BIOPROCESSES
Software packages for simulation of Bioprocesses - MATLAB-SIMULINK,SIMNON, ISIM;
Simulation of Bioprocesses using models from literature sources .
5. SIMULATION OF PLANTS
Simulation techniques in process design and integrated chemical plants and processes. Application
of simulation packages.

TEXT BOOKS:

1. Modelling and Control of Fermentation Processes, Edited by J.R. Leigh,
(1987), Peter Peregrinus, London.
2. Luyben W.L., “Process Modelling, Simulation And Control For Chemical
Engineers” 2/e,McGraw Hill International edition, 1990.
3. Process Computations in Bio-technology, T. K. Ghose (Editior), Tata Mcgraw Hill publ. Co., N.
Delhi.

REFERENCES:
1. Modelling and Optimisation of Fermentation Processes, Edited by B. Volesky
and J. Votruba, (1992) , Elsevier, Amsterdam
2. Smith C.A. and Corripio A.B., “Principles and Practice of Automatic Process
Control “, 1985, John Wiley & and Sons.
3. Morton M. Denn, “Process Modelling” , 1986, Longman Scientific &
Technical, England.
4. McNeil and Harvey, “Fermentation - A Practical Approach” (1990), IRL
Press., U.K.


MBC 208 BIO-PROCESS LABORATORY

PART A

1. Growth kinetics of bacteria, fungi.

2. Fermentation studies - Batch, fed-batch and Continuous cultivation

3. Solid state fermentation Techniques

4. Centrifugation,

5. Spray Drying

6. Freeze Drying

REFERENCE BOOKS :

1. Laboratory Manual in Microbiology, P.Gunasekaran, 1995, New Age
International (P) ,Ltd .N. Delhi.

2. Experiments in Microbiology ,Plant Pathology and Tissue Culture., K .R. Aneja,
1993, New Age International (P), Ltd ,N Delhi.

PART B

1. Gas analysis calculations and interpretations

2. Analytical techniques for estimation of glucose, ethanol, glutamate, acetate and
other metabolites

3. Modelling and Simulation of fermentation Process (Computer Lab)

Reference book:

1. McNeil and Harvey, “Fermentation - A Practical Approach” (1990), IRL
Press., U.K.


LIST OF ELECTIVES

MBCE 01
SEPARATION AND ANALYTICAL TECHNIQUES

1. INTRODUCTION TO BIOSEPARATION
Characterisation of Biomolecules and fermentation broths; Role of downstream processing in
biotechnology; Broad strategies for design of bio-separation processes.

2. CELL DISRUPTION, SOLID-LIQUID AND MOLECULAR SIZE-BASED
SEPARATIONS
Cell disruption - Mechanical and Chemical methods; Cake filtration and micro-filtration;
Centrifugation and sedimentation. Membrane processes - Dialysis, Ultra-filtration, Reverse
osmosis and Electro-dialysis.

3. LIQUID-LIQUID SEPARATIONS AND PROTIEN SEPARATIONS
Solvent extraction of small molecules; Aqueous two-phase extraction of proteins; Precipitation of
proteins with salts and with organic solvents

4. ADSORBPTION AND CHROMATOGRAPHIC SEPARATIONS
Adsorption processes - Principles of chromatographic separation - gel filtration, reversed-phase,
hydrophobic interaction, ion-exchange, IMAC and bio-affinity chromatography - Design and
selection of chromatographic matrices - Modes of operation; Design of Large-scale
chromatographic separation processes - Electrophoretic Separation processes.

5. FINAL PRODUCT PURIFICATION AND PREPARATION
Crystallization - Drying and Lyophilization - Formulation Strategies.

TEXT BOOKS:
1. J .Asenjo (Ed.) “Separation Processes in Biotechnology” (1993), Marcel-Dekker .

2. Verrall, M.S. and Hudson, M.J. “Separations for Biotechnology” (1990) Ellis Horwood.

3. Paul A Belter, E. L .Cussler and Wei Shan Hu “Bioseparation - Downstream Processing for
Biotechnology” (1988). Wiley Inter Science, N.York.

4. “Product Recovery in Bioprocess Technology” BIOTOL Series, (1992) , Butterworth
Heinemann .U.K.


MBCE 02
RECOMBINANT DNA TECHNOLOGY

1. BASICS OF RECOMBINANT DNA:

Role of genes within cells; elucidation of the genetic code; genetic elements that control gene
expression.

2. VECTORS FOR RECOMBINANT CELLS:

Plasmids, Cosmids, Phage vectors. Plasmids for production of bio-products

3. PRODUCTION OF RECOMBINANT DNA MOLECULES:

Methods of creating recombinant DNA molecules; restriction enzymes and mapping of DNA;
Plasmid and phage vectors.

4. APPLICATIONS OF RECOMBINANT DNA TECHNOLOGY:

Production of products from recombinant cells; Cloning in plants - Ti plasmid of Agrobacteria;
Transgenic animals.

5. ETHICAL ASPECTS ON USE RECOMBINANT DNA TECHNOLOGY:

Recombinant products - a bane or boon to society; safety guidelines of recombinant DNA research
and production; Successful recombinant DNA processes for production of high-value metabolites.

TEXT BOOKS:

1. Old R.W. and Primrose, S .B., “Principles of Gene Manipulation” Blackwell Scientific
Publications, 1983.

2. Milestones in Biotechnology, Edited by J .Davies and W .S. Reznikoff (1992), Butterworth
Heinemann, U.K

3. Desmond. S.T .Niccholl, ” An Introduction to Genetic Engineering “,1994,C L P Edition,
Cambridge University Press, U.K.


MBCE 03
FOODPROCESSING TECHNOLOGY

1. INTRODUCTION:

Biotechnology in relation to the food industry; Nutritive value of food; types of microorganisms
associated with food, its sources, types and behavior in foods.

2. FOOD PRESERVATION:
Preservation of meat, Fisheries, Vegetables, dairy products; Enzymes and Chemicals used in food
processing,. Thermal inactivation of micro-organisms; Thermal process evaluations; Freezing and
thawing of foods.

3. FERMENTED FOOD PRODUCTS:

Dairy products, meat, fishery, non beverage plant products, Beverages and related products of
baking.

4. FOOD SPOILAGE:

Food borne illness, quality control. Case studies on Biotechnology in the evolution of food quality,
HFCS (High fructose corn syrup) and Myco-proteins.

5. FOOD PROCESSING OPERATIONS:

Rheology and Fluid flow; Calculation of power requirements for pumping of food materials;
Material and energy balances in Food processing; Thermal processing of food; Microwave
heating; Biochemical engineering for flavor and food production

REFERENCES:

1. George J Banward, “Basic Food Microbiology” (1987), CBS Publishers , N.Delhi.

2. R .Angold, G .Beech and J. Taggart," Food Biotechnology", 1989, Cambridge University Press,
U.K.,

3. Lindsay, “Biotechnology challenges for the flavour and food industry” (1988), Elsevier Applied
Science

4. H.G.Muller,” An Introduction to Tropical Food Science”,1989,C L P Edition, Cambridge
University Press, U.K.


MBCE 04 ENZYME ENGINEERING

1. APPLICATION OF ENZYMES:

Classification of Enzymes, Commercial applications of enzymes in food, pharmaceutical and other
industries; enzymes for diagnostic applications.

2. IMMOBILIZATION TECHNIQUES:

Merits and demerits; Carrier types; Techniques of Immobilization; Fields of Application -
Immobilized enzymes as process aids and as analytical tools; Immobilization of co-enzymes-
Interaction - Chemical synthesis-Characterisation of coenzyme- analogues exploitation of binding
properties of coenzyme. Metal based Enzyme Crosslinking and Stabilization. Analysis of Film and
Pore Diffusion Effects on Kinetics of Immoblilized Enzyme Reactions; calculation of
Effectiveness Factors of IME systems

3. KINETICS AND MECHANISM OF ENZYME ACTION:

Kinetic Models; Types of Inhibitions; Inhibition kinetics; Modeling of rate equations for single and
multiple substrate reactions.

4. DESIGN OF ENZYME REACTORS:

Design and Operation of Ideal reactors - CSTR and PFR; Design of Packed-bed and Fluidised bed
Immobilised Enzyme Reactors; Membrane Reactors for immobilised enzyme systems.

5. DESIGN OF BIOSENSORS:
Use of enzymes in analysis - Types of sensing -gadgetry and methods; use of unnatural substrates -
artificial enzymes - enzyme mimicking. Enzyme Engineering in non-aqueous solvents. Case of
Subtlisin in Polar media. Extemozymes and Abzymes

TEXT BOOKS:
1. “Technological applications of Biocatalysts” BIOTOL Series, 1995, Butterworth Heinemann,
U.K.

2 A. Cornish- Bowden, “Analysis of Enzyme Kinetic data” , 1996, Oxford Univ. Press, U.K.
REFERENCES:
1. Wiseman A (Ed) “Topics in Enzyme and Fermentation Biotechnology” Volume 5, (1991), Ellis
and Harwood , U.K.


MBC E 05 PLANT CELL CULTURE

1. AN OUTLINE ON MOLECULAR BIOLOGY:
DNA Replication, translation, transcription. Fundamentals of recombinant DNA
technology, Gene regulation

2. AGROBACTERIUM & PLANT GENETIC ENGINEERING:
Agrobacterium mediated gene transfer and cloning. Types of plant vectors and
their use in gene manipulation.

3. PLANT VIRUSES:
Classification - diagnosis - remedy - viruses as a tool to deliver foreign DNA.

4. MOLECULAR BASIS OF DISEASE SUSCEPTIBILITY & RESISTANCE:
Transposable elements, Factors influencing disease resistance and susceptibility
RFLP.

5. TRANSGENICS:
Herbicide tolerance - insect resistance - viral resistance - stress tolerance -
Development of disease resistant plants by introducing B.t genes.

TEXT BOOKS & REFERENCES:

1. S. H. Mantell, J. A. Mathews, R.A. Mckee., “ Principles of Plant Biotechnology -
An Introduction to Genetic Engineering in Plants” Blackwell Scientific
Publications, 1985.

2. Dodds, J. H. “ Plant Genetic Engineering ” 1985, Cambridge University Press,
U.K.

3. J. Holden, J. Peacock, T. Williams, (Ed.), Genes, Crops and the Environment,
1993, C L P Edition, Cambridge University Press, U.K.

4. J.G .Manners,” Principles of Plant Pathology”, 2nd Ed., 1993, C L P Edition,
Cambridge University Press, U. K.

5. Dodds, J.H., and L.W. Roberts, (1985), Experiments in Plant Cell Culture (2nd
Ed.), Cambridge University Press., U.K.

6. Advances in Biochemical Engg./Biotechnology, Vol. 31,(1985), Plant Cell
Culture., Springer Verlag, Germany.


MBCE 06 ENVIRONMENTAL BIOTECHNOLOGY

1.WATER POLLUTANTS: SOURCES, EFFECTS, MONITORING AND
QUALITY STANDARDS:
Water Pollution Sources and Classification of water pollutants - Water pollution laws
and standards - Water pollution and health - Wastewater sampling and analysis. -
BOD, COD of wastewater - monitoring - Compliance with Standards.

8 hours
2.ANAEROBIC PROCESSES FOR B O D REDUCTION:
Anaerobic Digestion: Microbial And Biochemistry Aspects. High Rate Attached
Film Digesters. Sludge Disposal
8 hours
3.AEROBIC PROCESSES FOR B O D REDUCTION:
Aeration requirements - Microbial factors -Activated sludge and Attached film
processes.
8 hours
4.ADVANCED WASTEWATER AND WATER
TREATMENT:
Carbon Adsorption - Ion Exchange - Membrane Processes - Nutrient (nitrogen and phosphorus)
removal- Design of plant for treatment and disposal of sludge
8 hours

5. SOLIDS WASTE AND LANDFILL MANAGEMENT:

Sources and classification - methods of solid waste disposal - Composting (natural) -
Accelerated composting with industrial sludge - Landfill Technology -Methods
adopted for municipal solid waste-Toxic-waste management, Incineration of
industrial waste, Design aspects, economics.
8 hours
Text books:

1. Rittman, B. E. and McCarty, P., Environmental Biotechnology: Principles
and Applications,) McGraw-Hill International Edition, Singapore. (2000)
2. G. Tchbanoglous and F.L. Burton, Metcalf and Eddy‟s Wastewater
Treatment, Disposal And Reuse (Third Edition), Tata McGraw-Hill
publishing Co Ltd, N. Delhi. (1996)
3. A. P. Sincero and G.A. Sincero, Environmental Engineering: A Design
Approach, Prentice Hall of India pvt Ltd, N. Delhi. (1996)

Reference Books:

1. Microbiology for Environmental Scientists and Engineers, A.Gaudy and E.
Gaudy, McGraw Hill International Book Co, N.Y. (1981)
2. D. Barnes and P. J. Bliss , Biological Control of Nitrogen in Wastewater
Treatment, E & F N Spon, London.(1983)
4. Industrial Water Pollution Control, Third Edition, W. W. Eckenfelder Jr,
McGraw-Hill International Edition, Singapore. (2000)


MBCE 07 PHARMACEUTICAL TECHNOLOGY

1. INTRODUCTION

Development of Drugs and Pharmaceutical Industry - Organic therapeutic agents -
uses and economics

2. DRUG METABOLISM AND PHARMACO-KINETICS

Drug metabolism – Physico-chemical principles - Pharma Kinetics - Action of
drugs on human bodies

3. IMPORTANT UNIT PROCESSES AND THEIR APPLICATIONS

Chemical conversion processes - Alkylation - Carboxylation - Condensation and
Cyclisation - Dehydration, Esterification (Alcoholysis) - Halogenation - Oxidation
Sulfonation - Complex Chemical Conversions - Fermentation

4. MANUFACTURING PRINCIPLES

Compressed tablets - Wet granulation - Dry granulation or slugging - Direct
compression - Tablet presses Formulation - Coating - Pills - Capsules sustained
action dosage forms - Parental solutions - Oral Liquids - Injections - Ointments -
Standard hygiene and Good Manufacturing practice.

5. PHARMACEUTICAL PRODUCTS, ANALYSIS AND CONTROL
Vitamins - Cold remedies - Laxatives - Analgesics - External Antiseptics -
Antibiotics - Biologicals - Hormones - Preservation. Analytical methods and tests
for various drugs and pharmaceuticals. Packing Techniques - Quality control.

REFERENCES BOOKS :
1. Remington‟s Pharmaceutical Sciences, Mack Publishing Co, N.Y.
2. Medicinal Chemistry: Principles and Practice, Edited by F.D.King, (1994), The
Royal Society of Chemistry. U.K.
3. The Organic Chemistry of Drug Synthesis,D.Lednicer and L.A.Mitscher
(1977),John Wiley and Sons,N.Y.
4. The Discovery of Natural Products with Therapeutic potential, V.P .Gullo,
Butterworth Heinemann, U.K.
5. Essentials of Medicinal Chemistry , A .Korolkovas and J. H. Burckhalter (1976),
John Wiley and Sons,
N .Y.


MBCE 08
APPLIED BIOINFORMATICS

1. INTRODUCTION TO INFORMATION TECHNOLOGY
Computers and microprocessors, Storage devices, analysis and information handling
capacity. Informatics applied to Biotechnology: Data acquistion and storage; data
analysis; pattern matching, primary, secondary and tertiary structures of nucleic acids
and protein sequences.
2. NETWORKS AND DATA EXCHANGE
Process of MODEM, PAD, GPSS, networking modes for global data exchange;
local area wide area networks.
3. APPLICATION IN BIOTECHNOLOGY
Sources of nucleic acid and protein sequences - organization of information and
properties in the different data banks.
4. SEQUENCE DATABASE
EMBL, GENBANK, SWISS-PROT, etc., sequence databases

5. ALIGNMENTS
Global Vs Local alignments - Needleman and Wunsch global alignment algorithm - use
of scoring matrices - Use of PAM matrices for allowable amino acid susbstitutions and
detecting evolutionary relationships.

REFERENCES:

1. EMBL, GENBANK, SWISS-PROT Database Sequences.

MBC E 09 ANIMAL CELL TECHNOLOGY

1. INTRODUCTION
What is Biotechnology?; Scope of Biotechnology; Animal Biotechnology

2. MOLECULAR BIOLOGY
Nucleic acids - DNA, RNA, replication, transcription, translation genetic code, gene
regulation, genetic engineering, Plasmid isolation and gene cloning, Identification of
transformants.

3. CELL CULTURE TECHNOLOGY
Culturing of cells, Primary and secondary cell lines and the effect of growth factors, High
technology vaccines, Hybridoma technology.

4. GENETIC ENGINEERING
Enzyme technology, Somatic manipulation of DNA; Nucleic acid hybridisation and
probes in diagnosis; preparation of probes, evaluation and applications

5. APPLICATIONS
Rumen manipulation - probiotics - Embryo transfer technology - Invitro fertilization -
Transgenic animals.
REFERENCES:

1. C. A. Babiuk, J.P.Philips and Moo Young,M. “Animal Biotechnology”, Pergamon Press,
New York, 1989

MBCE 10 BIOENERGY

Faculty Member : Dr. H.J.Prabhu.
OBJECTIVE: To Learn about Biomass Conversion methods for energy production by
thermochemical and biochemical techniques and understand the design of gasifiers, combustors and biogas
generators.
1. BIOMASS CHARACTERISTICS & PREPARATION:
Biomass sources and classification. Chemical composition and properties of biomass. Energy plantations.
Preparation of biomass: Size reduction, Briquetting of loose biomass, Drying, Storage and handling of biomass.

2. BIOGAS TECHNOLOGY:
Feedstock for producing biogas. Aqueous wastes containing biodegradable organic matter, animal residues-
sugar rich materials. Microbial and biochemical aspects and operating parameters for biogas production.
Kinetics and mechanism. Dry and wet fermentation. Digestors for rural application-High rate digestors for
industrial waste water treatment.

3. PYROLYSIS AND THERMO CHEMICAL CONVERSION:
Thermo-chemical conversion of ligno-cellulose biomas. Incineration for safe disposal of hazardous waste.
Biomass processing for liquid fuel production. Pyrolysis of biomass-pyrolysis regime, effect of particle size,
temperature, and products obtained.

4. GASIFICATION OF BIOMASS:
Thermochemical principles: Effect of pressure, temperature and of introducing steam and oxygen. Design and
operation of Fixed and Fluidised Bed Gasifiers. Safety aspects.

5. COMBUSTION OF BIOMASS AND COGENERATION SYSTEMS:
Combustion of woody biomass-theory, calculations and design of equipments. Cogeneration in biomass
processing industries. Case studies: Combustion of rice husk, Use of bagasse for cogeneration.
TEXT BOOKS:
1. A.Chakraverthy, “Biotechnology and Alternative Technologies for Utilisation of Biomass or Agricultural
Wastes”, Oxford & IBH publishing Co., New Delhi, 1989.
2. K.M.Mital, “ Biogas Systems: Principles and Applications”, New Age International Publishers (p) Ltd.,
1996.
3. P.Venkata Ramana and S.N.Srinivas, “Biomass Energy Systems”, Tata Energy Research Institute, New
Delhi, 1996.
4. D.L. Klass and G.M. Emert, “Fuels from Biomass and Wastes”, Ann Arbor Science publ. Inc. Michigan,
1985.

REFERENCES:
1. Khandelwal K.C. and Mahdi, “Bio-gas Technology”, Tata McGraw-Hill pub. Co. Ltd., New Delhi, 1986.
2. O.P. Chawla, “Advances in bio-gas Technology”. I.C.A.R., New Delhi. 1970.

CONTENTS

Proposal and Introduction 2
Self assessment 3
Staff of Department of Chemical Engineering 4
Profile of Department of Chemical Engineering 5
Research Publications 6
Present Laboratory Facilities 8
Computer Networking and facilities 8
Fund Required to upgrade facilities 9
Staff required 9
Information on the Course 10
Annexure1: Copy of AICTE approval letter 14
Annexure 2: Central Grants Utilized for Facilities 19
Annexure 3: Library Facilities Enhanced 20
Annexure 4: Summary of Syllabus 21

PROPOSAL FOR SANCTION FOR CONDUCTING P.G. PROGRAMME OF M.TECH.
DEGREE IN BIOCHEMICAL ENGINEERING AT THE DEPARTMENT OF CHEMICAL
ENGINEERING, REGIONAL ENGINEERING COLLEGE, TIRUCHIRAPPALLI, 620
015, SUBMITTED TO THE MINISTRY OF HUMAN RESOURCE DEVELOPMENT,
DEPARTMENT OF EDUCATION, GOVERNMENT OF INDIA NEW DELHI- 110 001.
MARCH 2002.
REF: AICTE APPROVAL TO REGIONAL ENGINEERING COLLEGE DEPT. OF CHEMICAL
ENGINEERING , TIRUCHIRAPPALLI, 620 015, FOR CONDUCT OF P.G. COURSE IN
BIOCHEMICAL ENGINEERING FOR THE ACADEMIC SESSION 2002-2003 FOR THREE YEARS
PERIOD.(VIDE: AICTE LETTER, F.NO.: XVII-AIBPG/APP-1601/ET/2001 DATED FEBRUARY,12,
2002)

1. Name and Full Address of the Institution: Regional Engineering College,
Tiruchirappalli, 620 015
Tamil Nadu
Phone: 0431 - 500 061 extn. 2001
Fax: 0431 – 500 133
Cable : TREC
e-mail: prl@rect.ernet.in

2. Name of the Department: Department of Chemical Engineering
Phone: 0431 – 500 061 extn. 2901
e-mail: ssundar@rect.ernet.in

3. Conditions and Specific conditions for the AICTE approval of the Project:
[Copy enclosed; see Annexure 1.]

Degree: M.Tech

Course (specialization) : Chemical Engineering (Biochemical Engineering)

Annual Intake: 16

Duration(Full Time): 24 months

Additional 5 (Five) sponsored + 4(four) SC/ST candidates shall be admitted for the above
full time P G Programme.

INTRODUCTION
Bioprocess engineering contributes to many economic activities in our country such
as agricultural productivity, production of biologicals and antibiotics, biological wastewater
treatment, bioremediation for removal of contamination of land etc. Training of engineers in
this area at postgraduate level is necessary and would benefit many fields of human
endeavor. There is a good demand for highly qualified and trained engineers in this field.

SELFASSESSMENT REFLECTING COMPETENCE AND LEVEL
OF INFRASTRUCTURE AVAILABLE:
Department of Chemical Engineering has carried out several project works in this
field. The department is equipped with a biochemical engineering laboratory, one stirred
tank bioreactor (BIOSTAT-E) and a fluidized bed bioreactor with instrumentation. It has
some analytical facilities also. The department created the facilities by participating in the
MHRD sponsored schemes like I.I.T. (Madras) – R.E.C.(TRICHY) institutional network
scheme and Central Assistance Programme for Thrust Areas in Technical Education Scheme
(1988-1992), and subsequent projects granted by the AICTE.[See Annexure 2 for funds
utilized for biochemical and other facilities during 1988-2002]
Several projects have been carried out in this department in bioprocess engineering
among which some are applied to environmental pollution problems. Bio-desulfurisation of
di-benzothiophene found in diesel by bacteria was experimentally investigated to confirm the
capacity of this method to remove sulfur from this recalcitrant compound. Other projects
include three phase fluidized bed bioreactor for aerobic effluent treatment, bio-denitrification
of effluent and biodegradation of animal glue. A study on production of an important amino
acid has been carried out using a fluidized bed bioreactor for bacteria immobilized on beads.
Similar work on production of enzymes has been carried out.
A good number of reference volumes on Biotechnology/Biochemical Engineering
were added to our library. Back volumes of one journal were procured and three journals
were subscribed to from the funds provided. [See Annexure 3]
Currently one semester theory course on biochemical engineering are being taught to
U.G./ P.G. students of chemical engineering degree courses. Project works are carried out by
the U.G./ P.G students of chemical engineering, under guidance of the staff.
A. An M. Tech. Program in Biochemical Engg. has been approved in Febuary2002, by
the AICTE after an inspection visit of experts to the Dept. in Nov.2001
B. Expertise: The chemical engineering and chemistry faculty of this institution are well
qualified and competent, but complement of faculty is not adequate to run a full
fledged P G program.

Staff of Chemical Engineering:
Name and Designation of faculty Areas of Research

1. Dr. S. Sundaram Professor Process control,
Biotechnology
2. Dr.(Ms) S. K. Pandey Professor Heat transfer with
suspensions, Nucleate
Boiling
3. Dr. V. Venkata Ramani Professor Heat transfer with
phase change and
Instrumentation
4. Dr. H.J.Prabhu Asst. Professor Biochemical
engineering
5. Dr. P. Shanmugha Sundaram Asst. Professor Bio-process technology
6. Dr. N. Anantharaman Asst. Professor Fluidization,
Downstream processes
7. Dr. T. K. Radhakrishnan Asst. Professor Bio-process control,
Modeling
8. Dr. P. Siva Shanmugham Asst. Professor Bio-reaction
engineering, Equipment
Design
9. Dr. J. Sarat Chandra Babu Lecturer (senior) Modeling & Simulation
of bio-processes
10. Dr.(Ms) Meera S. Begam Lecturer Novel separation
techniques
11. Mr. M. Arivazhaghan Lecturer Environmental
biotechnology
12. Dr. (Ms).P. Kalaichelvi Lecturer Equipment design,
Process Engineering

Profile of the Department:

1 a) Name of the Institution REGIONAL ENGINEERING
COLLEGE, TIRUCHIRAPPPALLI
2 b) Name of the Department & year of CHEMICAL ENGINEERING: 1967
Establishment
c) Address for correspondence Dr. S. Sundaram, Professor & Head,
Department of Chemical Eng.,
R. E.C., Tiruchirappalli – 620 015
Phone (0431) 500181 Ext 2901 (O)
(0431) 500295®
email: ssundar@rect.ernet,in
3 Number of faculty members: Professors: 3
i) Professors; ii) Readers; iii) Asst. Professors: 5
Lecturers Lecturers: 4
4 Distinction earned by faculty members Dr.T. K. Radhakrishnan
like National and International Young scientist fellowship awarded
Awards, Professional Societies. &
Dr.(Mrs) S.K.Pandey awarded
Tamil Nadu Scientist Award both by
Tamil Nadu State Council for
Scientific Research
5 Student Strength at:
a) PG level – M. Tech. 48
b) Ph. D. in each sub-discipline
i) Plant Design Two
ii) Instrumentation and Control Two
iii) Energy Engineering One
6 Indicate the development grant received from UGC/ AICTE
Seventh Plan Eight Plan
Building 14.0 lac
Equipment - -
7 Annual grant to the department from your Institution during last two years
Year For Research For Teaching
1998-99 - Rs. 20 000/-
1999-2000 - Rs. 20 000/-
8 Details of research grant from different agencies during the last five years:
MHRD – R & D 2000 8.0 lakhs
AICTE-modernization 1999 6.0 lakhs
AICTE-R&D 1997 5.0 lakhs

Research publications:
List of Research publications, including patents, coming out of your department during the last five years in the
refereed journals

1. Sundaram, S. (1995) “ Modeling and optimizing dextrose fermentation using a fluorosensor, vol.31,
page 251, 32nd Annual MBS, Colorado.

2. Sundaram, S. (1996) “Modeling and optimizing citric acid production using a fluorosensor, 15 th
Biomedical Engineering Conference, University of Dayton, OHIO, March 29-31 (1996)

3. Sundaram, S., Saileja, D., Kalpana, N. (1998) Modeling and optimizing alcohol production by
fermentation of dectrose-xylose mixed feed using a fluorosensor, vol. 34, 35 th Annual RMBS,
Colorado (1998)

4. Saravanan, V, Hemachandran, Eugene Raj and Sundaram, S (2000) Liquid Phase Volumetric Mass
Transfer Coefficient “ Bio Process Eng. Accepted for publication

5. Prabhu H.J. (1991) ‟Studies on effect of additives on bio-methanation ‟ in Research
and Industry, vol 36,March 1991

6. Prabhu H.J. (1991) „Studies on biomethanation of water hyacinth‟, in Research &
Industry, vol 36, June 1991.
7. P. Sivashanmugam and S. Sundaram (1998) “Hydrodynamics of Annular Circulating Fluidised Bed
Drier”, Powder Technology, July, 1999 p. 196-198

8. P. Sivashanmugam and S. Sundaram “Hydrodynamics of Annular Circulating Fluidised Bed Drier with
Sand particles” accepted for publication in the Journal of Bioprocess Engineering

9. P. Sivashanmugam and S. Sundaram “Residence Time Distribution studies in Annular Circulating
Fluidised Bed Drier” accepted for publication in Powder Technology

10. P. Sivashanmugam and S. Sundaram “Studies on Improvement in Performance of Heat Exchanger
fitted with Twisted Tape” ASCE Journal 1999, 92-96.

11. Sarat babu and B. Pitchumani (1997) “Performance studies on uniflow cyclone” paper presented at
Filtech International Conference, DECHEMA, organized at Disseldoff, Germany Sept. 14-16, 1997.

12. Ramanan, P, P. Srinivasa Rao, Sarat chandra babu,J, and Pitchumani, B (1997) “Effect of fine particles
on flow properties” Bulk Solids Handling, vol.4, 1997.

13. Karthik Narayanan and N. Anantharaman (1999) “ RED studies on Semi-fluidization” presented at
CHEMCON-99, held at Punjab University, Chandigarh, from 20 Dec. to 23 Dec. 1999.

14. Balasubramanian, N. and Anantharaman, N (1995) Fluidized bed drying of Granular materials”, paper
presented at the IIChE‟95, Annual Session at Kalpak am, India, 1995.

15. Sivashanmugam, P and Prabhu H.J. (1998) „Production of citric acid from
commercial sugar by Aspergillus niger‟ , presented in t CHEMCON,1998

16. Sivashanmugam, P and Prabhu H.J. (1999) „Production of citric acid using a mixed
culture of yeast and Aspergillus niger‟ , presented in CHEMCON 1999

17. Sivashanmugam, P and Prabhu H.J.(2000) „Production of Alpha Amylase „,
presented in CHEMCON 2000

18. Prabhu H.J. and Sivashanmugam. P.(2001) „Fluidized bed Biomethanation of
wastewater‟, presented at International Workshop on Environment and Energy,
Periyar Maniammai College of Technology for Women (PMCTW),
Periyarnagar,Nov 2001

Present Laboratory Facilities:
A biochemical engineering laboratory of about Rs. 80 lakhs is already established in the
department
The equipments available are
a. Braun make BIOSTAT-E laboratory fermenter with instrumentation
b. Fluidised bed fermenter -BIOENGINEERING make.
c. Anaerobic Chamber and BOD incubater
d. Freeze Drier and Deep Freezer
e. Laminar flow chamber
f. HPLC – Hitachi (Isocratic)
g. Gas chromatograph – Nucon
h. Porosimeter – Carlo Erba
i. Fluorosensor
j. UV-VIS spectrophotometer-JASCO
k. Orbital shaker
l. Centrifuges

Computer networking in the Institute:
Computer center has local area network (LAN) of 250 terminals at the center in addition to 4 terminals to
each department. CAD laboratory has three workstations loaded with UNIX based software packages.

Computing facilities in the Department:

Department has recently procured 6 stand alone systems out of AICTE Modernization / R&D projects.
Software packages are needed.

Fund required to upgrade existing facilities:

Rs.lakhs

New fluidized bed fermentors -Two 16.00

Coreco Image analyzer for cell counting 4.00

Accessories for Biochemistry analyzer 5.00

Accessories for Lyophilizer 1.00

Accessories for HPLC (Hitachi) 3.00

Upgradation of GC:

(New columns + standard gas samples

+ computer interfacing) 3.00

Accessories for Mercury porosimeter 2.00

Subscription to biotechnology

International journals 6.00

Extension Block
(50m x 20m = 1 000 sq. m.) 10.00
--------
Total ( Nonrecurring, In Rs. Lakhs) 50.00
--------

Staff and Other recurring fund Requirements:
A) Teaching Staff:
Professor :One
Assistant Professor:One
Lecturer: One

B) Technical Staff:
Trained Laboratory
assistants: Three

Particulars of Recurring Amount (in lakhs of Rupees)

Salaries of faculty 6.36
Salaries of non-technical staff 1.28
Other recurring expenditure 1.60

Total Fund required:

Particulars Rs. lakhs
a) Recurring 9.74
b) Non-recurring 50.00

Grand Total 59.74

DETAILS ABOUT THE COURSE:
INFORMATI ON THE COURSE TO BE RECOGNISED / TO BE INTRODUCED

NAME OF THE DEPARTMENT: CHEMICAL ENGINEERING

1 Name of the course : M.Tech. Bio-chemical Engineering

Name of Name of Intake Year of Duration Name of degree
the degree specialisation. (full time) starting (total) & branch
eligible for
admission

M.Tech. Biochemical 25 2002 2 years 4 years B.E.
Engineering ( 4 semester) /B.Tech or
equiv. degree
in Chemical/
Biochemical/
Electrochemical
Engineering

2 Course structure and scheme of evaluation (semester - wise)

Name of the subject Hours per week Credits Evaluation (Marks)
L T P Internal Written Practical Total
Semester – I
Microbiology 3 3 100 100 200

Biochemistry 3 3 100 100 200

Biochemical Engineering 3 4 100 100 200

Biochemical Reaction 3 3 100 100 200
Engineering

Microbiology & 9 3 100 100 200
Biochemistry Laboratory
Elective – 1 3 3 100 100 200
(See Annexure iv)

Name of the subject Hours per week Credits Evaluation (marks
L T P Internal Written Practical/ Tota
Viva l
Semester - II
Bioprocess equipment 3 3 100 100 200
Design
Bioprocess Instrument. & 3 3 100 100 200
Control
Bioprocess Modeling and 3 1 4 100 100 200
Simulation
Bioprocess Laboratory 9 3 100 100 200

Elective - 2 3 3 100 100 200

Elective - 3 3 3 100 100 200
(See page 12)
Semesters III

Elective 4 3 3 100 100 200

Elective 5 3 3 100 100 200

Elective 6 3 3 100 100 200

Project Work 6 100

Semesters IV 12 200 200 400
Project Work (Viva voce)
Total Credits in the Course = 65

M.Tech. (Bio-chemical Engineering)- SUMMARY OF SYLLABUS

M. Tech. Specialization: Biochemical Engineerng
SEMESTER 1 SEMESTER 2
Code No: Title (core) Code No Title (core)
MBCH MBCH
101 Industrial Microbiology 201 Bioprocess Equipment
Design
102 Applied Biochemistry 202 Bioprocess Instrumentation
& Control
103 Bioprocess Engineering Principles 203 Modeling & Simulation of
Bioprocesses
104 Biochemical reaction Engineering 204 Bioprocess Lab
105 Microbiology & biochemistry Lab MBCHE** Elective Subject 2
MBCHE **
MBCH Elective Subject 1 Elective Subject 3
E**

M. Tech Specialization: Biochemical Engineering
SEMESTER 3 SEMESTER 4
Code Title (Elective) Code Title
No No
MBCHE ** Elective Subject 4
PROJECT WORK PROJECT WORK

CODE M. Tech Specialization: Biochemical Engineering
NO: TITLE OF ELECTIVE SUBJECTS
MBCH
E 01 Separation & Analytical Techniques
E 02 Recombinant DNA Technology
E 03 Food Processing
E 04 Environmental Biotechnology
E 05 Plant Cell Culture
E 06 Advanced Downstream Processes
E 07 Pharmaceutical Technology
E 08 Animal Cell Technology
E 09 Enzyme Technology

3. Method of admission to the course: GATE / Separate admission test

4. Facilities available in the Department for conducting the course

The department has a full fledged Bioprocess laboratory.

5. Supporting facilities available in the institution in the other departments:

Faculty from Chemistry, Instrumentation & Control Engineering, and Mathematics
Department are available for teaching the course.

6. Employment potential for those who will obtain the degree in the proposed course

The M. Tech. Biochemical Engineering degree envisaged will meet the demand for well
qualified young Biochemical Engineers to commercialize production of Biotech.
Products.

7. Additional Faculty and Other Staff Required if the Course is approved:

Professor Assistant Lecturer Technical Non-technical
Professor Supporting supporting
Staff staff

1 1 1 3 -

8. Additional fund requirements for the course

Particulars Amount (in lakhs of Rupees)

a) Recurring
1. Salaries of faculty 6.36
2. Salaries of non-technical staff 1.28
3. Salaries of supporting staff -
4. Scholarships -
5. Expenditure for travel -
6. Other recurring expenditure 1.60
Total 9.74

b) Non-recurring
1. Extension Block 10.00
50m x 20m = 1 000 sq. m.
2. Upgradation of
Laboratory Experimental 40.00
Facilities
3. Others Nil

Grand Total , Rs. Lakhs 59.74

9. Specify the funds required from AICTE/Ministry of HRD

a) Recurring 9.74 Rs. lakhs

b) Non-recurring 50.00 Rs. lakhs
Grand Total 59.74 Rs. lakhs

10. Details of Faculty already available for conducting the proposed courses.

Name of the course No. of Professors No. of Asst. No. of Lecturers Total
Professors
Ph.D. Ph.D. Ph.D. NonPh.D.
Biochemical Engineering 3 5 3 1 12

11. Please mention the source of fund for the proposed course:
Fund required from the approved scheme of financial assistance for PG courses
(MHRD / AICTE/ DST (FIST- Level –1) and sponsored projects would be utilized.

12. Details of the UG courses (already being conducted in the department proposing the
course) which is relevant to the proposed course.

Name of the AICTE approval Intake Year of Duration Name of Degree/
degree no. and date starting (Total) Branch eligible
for admission

B.Tech. in F.2-15/B-111/RC- 36 1967 4yrs N.A.
Chemical Engg. MB/93
21-5-94

1. Signature of the Head of the Department

Name : Dr. S.Sundaram

2. Signature of the Head of the Institution

Name : Dr.

Annexure 2

Central Grants Utilized for Facilities

Name of project Sanction letter year Amount
No & date awarded /
spent Rs.Lakhs

IIT-REC(Biochemical F 4-1 / 87-T7 1988 5
Engg.Laboratory)
dt.2-3-88

IIT-REC(Biochemical F 2-1/ 88-T7 1989 5
Engg.Laboratory)
dt.17-1-89

Central Assistance,Thrust F1-2/88T7 1989 10
Area(Biotechnology)
Dt.17-1-89

Central Assistance,Thrust F1-6/90-TD6 1991 10
Area(Biotechnology)
Dt. 25-1-91

Details of research grant from different agencies during the
years 1993 to 2002:
Source Year Amount of Grant
MHRD – R & D 2000 8.0 lakhs
AICTE-modernization 1999 6.0 lakhs

ANNEXURE-3
Library Facilities Enhanced
I Back volumes of the journal, Bioengineering and Biotechnology (upto 1987) were added to the Central
library of the institution at a cost of about Rs. 29000/= during 1990-91. The following International
Journals in biotechnology were subscribed: a) Journal of Fermentation and Bioengineering b) Journal
of Biotechnology c) Biomass
II Reference Books made available during the last ten years:
1. Biotechnology, Volumes 1 to 8.Edited by H J Rehm and G. Reed, First Edition, Verlag Chemie, FRG
(1985-86)
2. Comprehensive Biotechnology, Volumes 1 to 4. Edited by Murray Moo Young, Pergamon Press
(1984-85)
3. Advances in Biochemical Engg. / Biotechnology, Volumes 22 to 32, Springer Verlag (Annual
Volumes)
4. Biotechnologies:Challemges and promises-Albert Sasson-UNESCO (1984)
5. Precott and Dunn‟s Industrial Microbiology (4 th Edition), Edited by G. Reed, Macmillan (1992)
6. Bio-reactor Immopbilised Enzymes and Cells: Fundamentals and Applications, Ed. By Murray Moo
Young, Elsevier Applied Sciences (1988)
7. A Revolution in biotechnology, Ed. By J. L. Marx, Cambridge University Press, Cambrdidge, (1989)
8. Handbook of Enzyme Biotechnology Ed. By A. Wiseman, Ellis Horwood, Chichester (1985)
9. Immobilized Cells and Enzymes: A Practical Approach, J. Woodward, IRL Press (1985)
10. Process Engineering Aspects of Immobilized Cell System. Institution of Chemical Engineers, U.K.,
(1986)
11. Microbiological Methods (5th Edition) C.H. Collins and P. M. Lyne,
Butterworths, (1985)
12. Bioprocess Technology: Kinetics and Reactors A. Moser, Springer Verlag (1988)
13. Enzymes by Dixon and Web, (Third Edition) Academic Press(1979)
14. Fundamentals of Biotechnology, Edited by P. Prave, VCH, Weinheim (1987)
15. Bioreactors and Bio-transformations Ed. By G.W. Moody and P.B. Baker, Elsevier Applied Science
(1987)

PROPOSAL FOR CONDUCTING
M.TECH. DEGREE COURSE IN
NATIONAL INSTITUTE OF TECHNOLOGY,
TIRUCHIRAPPALLI, 620 015

DEPARTMENT OF CHEMICAL ENGINEERING

BIOCHEMICAL ENGINEERING

2004 - 2005

(JANUARY, 2004)
SUBMITTED TO
MINISTRY OF HUMAN RESOURCE DEVELOPMENT
DEPARTMENT OF EDUCATION
GOVERNMENT OF INDIA
NEW DELHI- 110 001

MODERNISATION AND REMOVAL OF OBSOLECENCE

1. Name and Full address with : NATONAL INSTITUTE OF
with pin code of the institution TECHNOLOGY
Tiruchirapalli-620015, Tamil Nadu

2. Title of Research Project : MODERNISATION AND REMOVAL OF
OBSOLESCENCE IN BIOCHEMICAL
ENGINEERING LABORATORY

3. Name of the Department : CHEMICAL ENGINEERING

4. (i) Name and Designation of
Principal Investigator : Dr.S.Sundaram
Professor and Head
(ii) (ii) Name and Designation of
Co- Investigator : Dr. H.J.Prabhu
Assistant Professor

(iii) Name and Designation of
Co- Investigator : Dr. P.Sivashanmugam
Assistant Professor

5. Total cost of the Project : 22.00lakhs

.

MODERNISATION AND REMOVAL OF OBSOLECENCE

1. Name and Full address with : NATONAL INSTITUTE OF
with pin code of the institution TECHNOLOGY
Tiruchirapalli-620015, Tamil Nadu

2. Title of Research Project : MODERNISATION AND REMOVAL OF
OBSOLESCENCE IN BIOCHEMICAL

3. Name of the Department : CHEMICAL ENGINEERING

4. (i) Name and Designation of
Principal Investigator : Dr.S.Sundaram
Professor and Head
(ii) (ii) Name and Designation of
Co- Investigator : Dr. H.J.Prabhu
Assistant Professor

(iii) Name and Designation of
Co- Investigator : Dr. P.Sivashanmugam
Assistant Professor

5. Total cost of the Project : 22.00lakhs

6. Details of Projects supported by the MHRD/AICTE during 1998/99 to 2002-03:
[Please See Annexure –1]

7. Summary of the Project:

a. Objectives of the Project:

To provide facilities for experimental courses and research projects in biochemical
engineering and biotechnology topics it is necessary to modernize the laboratory. To
include experimental courses along with the theory courses provided at present at
both the U.G, and P.G. levels, the additional facilities are needed. This will improve
our capacity to undertake consultancy projects also.

b. Methodology to be adopted to achieved these objectives:

A committee comprising of the Principle Coordinater and Co-coordinators, Head of
the Department, and the Dean, Planning and Development, will review the progress
once in six months.

c. Monitoring mechanism and self assessment:

An assessment of the utilization of the facility for the objectives proposed will
be made with feedback from the students and project investigators. Review will
indicate the progress of the project.

d. Stipulated Period of completion of the project: Two Years.

8. Introduction:
a. Present state of art:

The existing laboratory was established nearly a two decade back and was
used for carrying out experimental projects. Modern equipments and analysis
methods can enhance the utility of the facilities for regular lab course work as well
as for consultancy projects. Also, computer interfacing of the instrumented fermenter
for monitoring and control of parameters needs additional input.

b. Funding provided from other sources: Nil

c. Present level of infrastructure:

At present, a laboratory is available with a five liter capacity fermenter with
monitoring facilities. A laminar air flow chamber for inoculums handling,
laboratory autoclave, centrifuge, BOD incubator and muffle furnace are available.
An orbital shaker, a gas chromatograph and a spectrophotometer are available in
the laboratory.

9. Budget:

Name of the equipment Cost,

Rs. lakhs

1.Electrodes for dissolved oxygen and pH, 1.00

for fermented (autoclavable)

2.Laboratory sterilizer with control 3.00

3.Mini Jar Fermentor 5.00

4. Laboratory pH and ion analyzer with electrodes 3.00

5.Peristaltic pumps of varying capacity 1.00

6. Accessories for gas chromatograph 1.00

7. Glucose analyzer with Biosensor 5.00

8. Computer Networking facilities 3.00

Total 22.00

10. Summary sheet

1. Name of the Institution : NATIONAL INSTITUTE OF
TECHNOLGY
TIRUCHIRAPALLI-620015

2. Title of the project : MODERNISATION AND REMOVAL
OF OBSOLESCENCE IN BIOCHEMICAL

3. Name of the Department : CHEMICAL ENGIEERING

4. Cost of the Project : Rs 22lakhs

5. Amount Released Earlier : Nil

6. Utilization Position in respect of : Refer Annexure-I
grants released earlier
(up to 2000-2001)
for various projects
(Details to be given Project-wise)

7. Reason for unspent balance : Project period is not yet
Completed

8. Name of the Principal Investigator : Dr.S.Sundaram

9. Name of the Co-investigator : Dr.H.J.Prabhu and Dr.P.Sivashanmugam

FOR OFFICIAL USE ONLY

1. Name of the area :

2. Recommendations :

1) Approved

2) Not Approved

3) Deferred

4). Transferred to area Amount
(Rs. In lakhs)
3. Remarks if any

11. Certificate

Certified that

i) the Principal Investigator is not due to retirement during the currency
of the Project

ii) the Principal Investigator is regular employee of this institution.
However in case he/she proceeds on sabbatical/ resigns/proceeds on
VRS etc, the institute will ensure to replace PI by a compatible
academician to ensure that without any break whatsoever, project will
be completed within the stipulated period of 2 years.

iii) It will be ensured that the implementation will be carried out on
mission mode with no time or cost over run and we are aware that
ministry will neither provide any extension of time nor additional
funding

iv) No over heads will be charged by the institution for this project and all
facilitation including other essentials/infrastructure support like air-
conditioning etc will be provided by the institution

Seal of the Institution Signature of Head of Institution

Annexure-I

Project Title Name of Co-ordinator Project Cost Project Status

1.Non-linear Mr.T.K.Rathakrishnan 5.0 lakhs Completed
control of F.No.8017/RD
Chemical reactors II/BOR/95/RCHE
6823/REC 90/DT 11-3-
1999
2.Modernization of Dr.N.Anantharaman 6.0 lakhs Completed
unit operation Lr.No-F 23-10-1198 TS-1
laboratory dt 14-1-98(MODROBS)
3.Study of Gas- Dr.S.Sundaram F.26- 8.0 lakhs Project Time
Solid distributive 4/99,TS-1dt 13/3/2000 Period is not yet
and dynamic flow over
regimes in dense
flow pneumatic
Conveying
4. Modernisation Dr.P.Sivashanmugam,F.28- 10.0 lakhs Project Time
of Chemical 3(24)/2002-TS.V dt 19-03- Period is not yet
Reaction 2002 over
Engineering
Laboratory
5. Heat Transfer Dr.Mrs.S.K.Pandey, F.26- 10.0 lakhs Project Time
studies during 1/2000 T.SV,April-2001 Period is not yet
Nucleate Pool over
boiling
6. CFD Modeling Dr.Mrs.S.K.Pandey, F.27- 10.0 lakhs Project Time
of Chemical 1/2002 T.SV,dt 19-03-2002 Period is not yet
Process Equipment over
6. Studies on Dr.K.M.Meera Sheriffa 10.0 lakhs Project Time
Emulsion Liquid Begum, F.26-4/2002. TS.V Period is not yet
Membrane dt 31-032003 over
Extraction
7.

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