DNA quantification

1. Biotechnology
TOPIC NAME
NUCLEIC ACID QUANTIFICATION BY FLOURIMETER

2. DNA & RNA Quantification via Fluorometric Analysis:
Nucleic acid quantification,encomassing Double stranded DNA(dsDNA), Single
stranded DNA(ssDNA) and RNA quantification,
Is a staple process in life science applications requiring and knowledge of
sample concentration Prior to downstream and experiment s.
Both type of nucleic acid are typically quentized
Using one of two optical technologies:

3.  Ultraviolet Visible spectroscopy
 Fluorescence measurements
Accurately quantifying nucleic acid concentrations is critical for downstream
applications such as next-generation sequencing, transfection, PCR and cloning. Such
applications frequently require a target range of nucleic acid concentration and purity
for optimum performance and inaccurate determination of concentrations can cause
higher variability or assay failure.

4. The Principals of FluoromeAnalysislysis:
Fluorometric analysis provides sensitivity at around 1,000 times higher than absorbance-based methods.
It is also a feature of fluorescence assays that they are highly specific to the analyte of interest compared to
those absorbance.
Quantification of DNA, RNA or Protein through fluorometric methods require the binding of analyte-
specific fluorophores to the sample of interest. The sample/fluorophore complex is illuminated with a
specified excitation wavelength from a light source. The fluorophore emits light over a characteristic spectrum
and the amount of emitted light is proportional to the amount of analyte in the sample. The light is captured
and recorded as relative fluorescent units (RFUs). The concentration of unknown samples can be understood
through the correlation to a standard curve of known concentration samples.

5.  Fluorometric Analysis Equipment from DeNovi:
The DS-11 Series and QFX fluorometer from DeNovix are at the forefront of
fluorometric analysis equipment, being more sensitive and reproducible than any other
instrument in its class. The QFX is a powerful solution for fluorometric applications where
improved sensitivity or specificity is required while the DS-11 Series combines microvolume and
cuvette absorbance with fluorescence for total sample quantification method flexibility.
DeNovix Fluorometers have four fluorescent channels for quantification enabling compatibility
with all major quantification assays as well as future-proofing labs for future assay
developments.

6. Using the DeNovix Fluorescence quantification assays, researchers can
quantify dsDNA between 0.5 pg/µL and 4000 ng/µL and a single RNA assay quantifies
in the range of 250 pg/µL to 1500 ng/µL. Each assay is well-suited solution for
quantification of degraded or low concentration samples, even with common
contaminants present.
If you would like to find out more about the fluorometric analysis solutions that
DeNovix can offer, please contact us today.