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By:- Abhishek Singh Rana
Rkgit(Pharmacy)
Supercritical fluid
extraction technology
About Supercritical Fluid
Extraction
 Supercritical Fluid Extraction (SFE) is the
process of separating one component (the
extractant) from another (the matrix)
using supercritical fluids as the extracting solvent
 SFE can be used as a sample preparation step
for analytical purposes.
 SFE can be used on a larger scale to either strip
unwanted material from a product
(e.g. decaffeination) or collect a desired product
(e.g. essential oils)
Contd…
 These essential oils can include limonene and other
straight solvents.
 Carbon dioxide (CO2) is the most used supercritical
fluid, sometimes modified by co-solvents such
as ethanol or methanol.
 Extraction conditions for supercritical carbon
dioxide are above the critical temperature of 31 °C
and critical pressure of 74 bar.
 Addition of modifiers may slightly alter this.
Three phase of Supercritical Fluid
Advantages
 3 S comply with environmental standards
Reduce-energy conservation and reduce pollution; Reuse-
repeated, repeated use; Recycle-sustainable resources,
recycling.
 Inexhaustible Carbon dioxide is in fact industrial by-
products, such as the fermentation industry, the
petrochemical industry in the process will have a high
concentration of carbon dioxide, the collection of purified,
liquefied compressed can be stored after use.
 sterile and can inhibit bacterial growth
 non-toxic, safe
Principle
 Supercritical CO2 extraction means CO2 goes to a
supercritical phase after pressurization and heating
temperature above critical points.
 CO2 at supercritical phase have solubility powers
similar to liquid organic solvents, but with higher
diffusivities, higher transfer efficiency, lower
viscosities, and lower surface tension.
Contd…
 With advantages of non-toxic, colorless, odorless,
incombustibility, non- photochemical reaction, eco-
friendly and easy recycling, CO2 is now considered as
the best solvent for Supercritical Fluid Extraction
technology.
Advantages
●Effectively bioactive ingredients extraction with lower
viscosity and higher penetration to the matrix
●No solvent residue in the extract. This means lower
operating costs for clean-up and the reduction in post-
processing steps.
● Non-toxic, high safety degree, non-flammable, non-
explosive.
●Selective extraction by manipulating the operating
conditions (temperature, pressure
Applications
 Food science - Fat and oil samples in meat, egg, meals,
chocolate, dairy products, seeds and food snacks,
Natural products
 Flavors & spices of ginger, eucalyptus, soyabean,
coffee, soybean, basil, lime peels, potato chips,
popcorn By-products recovery
 Fruit and vegetable waste
Extraction of Neutraceuticals
from various sources
About
 Plants contain a broad range of bioactive compounds
such as lipids ,photochemical ,pharmaceutics , flavors
, fragrances and pigments.
 Plant extracts are widely used in the food,
pharmaceutical and cosmetics industries.
 Extraction techniques have been widely investigated
to obtain such valuable natural compounds from
plants for commercialization. Traditional methods,
such as Soxhlet extraction, which have been used for
many decades, are very time consuming and require
relatively large quantities of solvents
Contd..
 . There is an increasing demand for new extraction
techniques with shortened extraction time, reduced
organic solvent consumption, and increased pollution
prevention.
 Novel extraction methods including:-
 ultrasound-assisted extraction,
 microwave-assisted extraction,
 supercritical fluid extraction, and
Ultrasonic Extraction of Caffeine
 It is an effective method for the extraction of caffeine
and from coffee.
 Powerful ultrasonic devices assist the extraction
process whilst maximizing yield and shortening
processing time.
 Coffee – made from roasted coffee beans – is a very
popular drink that is worldwide consumed.
Extraction Techniques
 Ultrasonic extraction is a frequently used technique for
the extraction of plant materials using liquid solvents and
is proven for a fast and more complete extraction process in
comparison with traditional methods because the surface
area between the solid and liquid phase is significantly
larger due to the cell disruption and particle dispersion.
 Microwave-assisted extraction (MAE) is a process of
using microwave energy to heat solvents in contact with a
sample in order to partition analytes from the sample
matrix into the solvent. The ability to rapidly heat the
sample solvent mixture is inherent to MAE and the main
advantage of this technique.
Regulatory aspects of
Neutraceutical products in
India
About
 Pharma companies like Novartis, Glaxosmithkline and
Cadila healthcare has stepped into the dietary supplement
industry in recent years.
 The FMCG (fast moving consumer goods) suppliers and
pharmaceutical companies are the key players in the Indian
Nutraceutical market.
 Vitamin and mineral supplements are responsible for the
64% of the total Nutraceutical market in India
 The pharmaceutical companies are focusing on expanding
the market of already existing Nutraceutical product by
boosting it to the consumers rather than concentrating on
the innovation and development of new Nutraceutical
products.
Food safety standard and
standards act(FSSA)
 This act is laid down in 2006 in order to form the
statutory body FSSA which regulates the manufacture,
storage, distribution, sale and import, to ensure the
availability of the food and food products within the
country.
 Nutraceutical are grouped under the umbrella of
Foods by the FSS act 2006, rules and regulations 2011.
Section 22(1) of FSSA, defines “foods for special
dietary uses or functional foods or Nutraceutical
or health supplements”
Registration of Nutraceuticals Process in India
 Documents to be enclosed with new application for
license/import license to State/Central Licensing
Authority.:-
 Form-A
Form B
Blueprint/layout plan of the processing unit List of
Directors
Name and List Machineries to be used in the process
Photo I.D and address proof
Contd…
List of food category desired to be manufactured.
Authority letter with name and address of responsible
person Analysis report
Proof of possession of premises
Partnership deed/Affidavit/Memorandum & Articles
NOC from manufacturer
Food safety management system plan or certificate
Source materials
.

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Development and marketing of neutraceutical products

  • 1. By:- Abhishek Singh Rana Rkgit(Pharmacy)
  • 3. About Supercritical Fluid Extraction  Supercritical Fluid Extraction (SFE) is the process of separating one component (the extractant) from another (the matrix) using supercritical fluids as the extracting solvent  SFE can be used as a sample preparation step for analytical purposes.  SFE can be used on a larger scale to either strip unwanted material from a product (e.g. decaffeination) or collect a desired product (e.g. essential oils)
  • 4. Contd…  These essential oils can include limonene and other straight solvents.  Carbon dioxide (CO2) is the most used supercritical fluid, sometimes modified by co-solvents such as ethanol or methanol.  Extraction conditions for supercritical carbon dioxide are above the critical temperature of 31 °C and critical pressure of 74 bar.  Addition of modifiers may slightly alter this.
  • 5. Three phase of Supercritical Fluid
  • 6. Advantages  3 S comply with environmental standards Reduce-energy conservation and reduce pollution; Reuse- repeated, repeated use; Recycle-sustainable resources, recycling.  Inexhaustible Carbon dioxide is in fact industrial by- products, such as the fermentation industry, the petrochemical industry in the process will have a high concentration of carbon dioxide, the collection of purified, liquefied compressed can be stored after use.  sterile and can inhibit bacterial growth  non-toxic, safe
  • 7. Principle  Supercritical CO2 extraction means CO2 goes to a supercritical phase after pressurization and heating temperature above critical points.  CO2 at supercritical phase have solubility powers similar to liquid organic solvents, but with higher diffusivities, higher transfer efficiency, lower viscosities, and lower surface tension.
  • 8. Contd…  With advantages of non-toxic, colorless, odorless, incombustibility, non- photochemical reaction, eco- friendly and easy recycling, CO2 is now considered as the best solvent for Supercritical Fluid Extraction technology.
  • 9. Advantages ●Effectively bioactive ingredients extraction with lower viscosity and higher penetration to the matrix ●No solvent residue in the extract. This means lower operating costs for clean-up and the reduction in post- processing steps. ● Non-toxic, high safety degree, non-flammable, non- explosive. ●Selective extraction by manipulating the operating conditions (temperature, pressure
  • 10. Applications  Food science - Fat and oil samples in meat, egg, meals, chocolate, dairy products, seeds and food snacks, Natural products  Flavors & spices of ginger, eucalyptus, soyabean, coffee, soybean, basil, lime peels, potato chips, popcorn By-products recovery  Fruit and vegetable waste
  • 12. About  Plants contain a broad range of bioactive compounds such as lipids ,photochemical ,pharmaceutics , flavors , fragrances and pigments.  Plant extracts are widely used in the food, pharmaceutical and cosmetics industries.  Extraction techniques have been widely investigated to obtain such valuable natural compounds from plants for commercialization. Traditional methods, such as Soxhlet extraction, which have been used for many decades, are very time consuming and require relatively large quantities of solvents
  • 13. Contd..  . There is an increasing demand for new extraction techniques with shortened extraction time, reduced organic solvent consumption, and increased pollution prevention.  Novel extraction methods including:-  ultrasound-assisted extraction,  microwave-assisted extraction,  supercritical fluid extraction, and
  • 14. Ultrasonic Extraction of Caffeine  It is an effective method for the extraction of caffeine and from coffee.  Powerful ultrasonic devices assist the extraction process whilst maximizing yield and shortening processing time.  Coffee – made from roasted coffee beans – is a very popular drink that is worldwide consumed.
  • 15. Extraction Techniques  Ultrasonic extraction is a frequently used technique for the extraction of plant materials using liquid solvents and is proven for a fast and more complete extraction process in comparison with traditional methods because the surface area between the solid and liquid phase is significantly larger due to the cell disruption and particle dispersion.  Microwave-assisted extraction (MAE) is a process of using microwave energy to heat solvents in contact with a sample in order to partition analytes from the sample matrix into the solvent. The ability to rapidly heat the sample solvent mixture is inherent to MAE and the main advantage of this technique.
  • 17. About  Pharma companies like Novartis, Glaxosmithkline and Cadila healthcare has stepped into the dietary supplement industry in recent years.  The FMCG (fast moving consumer goods) suppliers and pharmaceutical companies are the key players in the Indian Nutraceutical market.  Vitamin and mineral supplements are responsible for the 64% of the total Nutraceutical market in India  The pharmaceutical companies are focusing on expanding the market of already existing Nutraceutical product by boosting it to the consumers rather than concentrating on the innovation and development of new Nutraceutical products.
  • 18. Food safety standard and standards act(FSSA)  This act is laid down in 2006 in order to form the statutory body FSSA which regulates the manufacture, storage, distribution, sale and import, to ensure the availability of the food and food products within the country.  Nutraceutical are grouped under the umbrella of Foods by the FSS act 2006, rules and regulations 2011. Section 22(1) of FSSA, defines “foods for special dietary uses or functional foods or Nutraceutical or health supplements”
  • 19. Registration of Nutraceuticals Process in India  Documents to be enclosed with new application for license/import license to State/Central Licensing Authority.:-  Form-A Form B Blueprint/layout plan of the processing unit List of Directors Name and List Machineries to be used in the process Photo I.D and address proof
  • 20. Contd… List of food category desired to be manufactured. Authority letter with name and address of responsible person Analysis report Proof of possession of premises Partnership deed/Affidavit/Memorandum & Articles NOC from manufacturer Food safety management system plan or certificate Source materials
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