This document summarizes the detection of swine hepatitis E virus (HEV) in pig livers in Jeju Island, South Korea. Samples from pig livers showed mild to moderate hepatitis and yellowish discoloration. RT-PCR testing detected swine HEV infection in the liver tissue. The findings suggest that swine can be a reservoir for zoonotic HEV infection, posing a risk to those with occupations exposed to pigs.

1. Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island

2. Nonenveloped Fecal-oralroute Clinicalsigns: jaundice, anorexia, nausea, hepatomegaly Exertssevereeffectsinpregnantwomen HepatitisE Virus

3. Zoonotic pigsnotobviously ill, displayverymildliverlesions on histopathologicexamination widespreadthroughouttheworld Antibodiesto HEV inpigsfromcountrieswhere HEV isendemicand manyindustrializedcountries Pigasanimalreservoir host for HEV HEV inpigs

4. Animalsamples

5. All liversampleswerecollectedfrompigs at necropsy One-thirdfrozen at -70℃ remainingpart fixedin 10% neutralbufferedformalin embeddedinparaffinwax cutinto 3 µmsections stainedwithhematoxylin and eosin Tissueprocessing

6. RT-PCR findings

7. Moderate to severe multifocal lymphoplasmacytic and histiocytic hepatitis or portal inflammation

8. mild hepatic enlargementand scattered yellowish discoloration foci

9. focal infiltration of lymphocytes, plasma cells, and macrophages in hepatic sinusoids and the portal triad

10. swineHEV infectionoccurs at a veryyoungage (under 2 months) inJejupigs. varioushepatitis virus antigenshavebeendetectedinlivertissue However, these viral markers are onlytransientlyexpressedduringtheincubation and earlysymptomaticperiodsofacute viral hepatitis and maynotbe present duringactivedisease immunohistochemistryisnotparticularlyuseful PCR methodmoresensitive and applicableas a specificassayforthediagnosisofvariousinfectiousdiseases individualswithswine-associatedoccupations at possible risk ofzoonoticinfection. Conclusions