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Detection of Eye Disorders
Through Retinal Image
Analysis
Blood Vessel Segmentation, Optic Disc Segmentation
and Fuzzy Logic Image Processing
By
Rahul Dey
Overview of the Presentation
 Common Eye Disorders
Blood and Optic disk Segmentation
 Literature Survey
 Algorithm
 Simulation
Fuzzy Logic Image Processing
 Introduction
 Fuzzy Inference System
 Implementation on Edges
2
Common Retinal Eye Disorders 3
Fig .2 GlaucomaFig.3 Diabetic Retinopathy
Source : www.fau.de
• Glaucoma is associated with elevated pressure in eye which
damages optic nerve
• DR is a common retinal complication associated with diabetes
Fig.1 Normal Eye
Literature Survey For Optic Disk
Segmentation
Extraction of optic disk, fovea, and blood vessel are used for
comprehensive analysis and grading of diabetic retinopathy
Other symptoms which can be detected are cotton wool spot,
Microaneurysms and haemorrages.
Methods :
 Circular hough transformation for
detection optic disk
 Curvlet transformation
 Artificial neural network
Source : www.fau.de
4
Literature Survey For Blood Vessel
Segmentation
Blood vessel provides nourishment to retina while diabetes
may weaken and leak blood vessel forming dot like
haemorrages
These leaking vessels often lead to swelling and decreased
vision
Blood vessels are segmented to locate optic disk and fovea
5
Fig.4 Segmented Blood vessel
Algorithm 6
Read image & set threshold for , blood vessel segmentation and
optic disk dilating window
Blood vessel segmentation starts
 Resize image to 576 × 576
 Read green channel because green channel has the highest contrast
 Performing morphological operation to highlight blood vessel
𝐶 = 𝐴 ⊕ 𝐵2 ⊖ 𝐵2 − (𝐴 ⊕ 𝐵1) ⊖ 𝐵1
 𝐵1, 𝐵2 having size 1 to 6
 Adaptive histogram equalization
 Gaussian filtering ( 𝜎 = 2 )
 Median filtering having kernel size 2 x 2
 Binarization with user defined threshold
Algorithm (contd..)
 Thinning operation
 Median filtering having kernel size 2 x 2
 Filling and dilation
 End of blood vessel segmentation
 Optic disk segmentation starts
 Read image
 Extract red plane
 Extract green plane
 Read template of user defined size
 Extract red plane of the template
 Do normalize correlation
𝑖=1
𝑀
(𝑇 𝑖− 𝑇)(𝐼 𝑖,𝑣− 𝐼)
𝑖=1
𝑀 (𝑇𝑖 − 𝑇)2
𝑖=1
𝑀 (𝐼𝑖,𝑣− 𝐼 𝑣)2
7
Algorithm (contd..)
 If
 Correlation co-efficient is > user defined threshold then extract
optic disk
 Dilate extracted optic disk with square window of 3 x 3 to 4 x 4
 Take mean value of the dilated image as threshold
 Binarize the image
 Median filter of size 3 x 3 to 4 x 4
 Open by taking kernel size of 4 x 4
 Fill the image
 Perform close operation on the image with disk shape kernel of radius 2
to 3 pixel
 Show image
 Canny edge detection
 Else
 Display error message
 End of Optic disk segmentation
8
Simulation Output 9
Difference Between Proposed &
Our Methodology
Proposed Our
• Gaussian filtering is not done • Additional Gaussian filtering done
• Green channel used for optic disk
localization
• Red channel used for optic disk
localization
• Green channel used for optic disk
segmentation
• Red channel used for optic disk
segmentation
• Filtering kernel size information are
missing
• All filtering sizes are computed
10
Blood Vessel Result Comparison 11
Optic Disk Comparison 12
Fuzzy Logic Image Processing(FLIP)
 Fuzzy image processing is a combination of fuzzy approach
to image processing.
 Fuzzy image processing stages:
 After the image data are transformed from gray-level plane
to the membership plane (fuzzification), appropriate fuzzy
techniques modify the membership values.
13
Applications of Fuzzy Logic Image
Processing
 Contrast Enhancement
 Edge Detection
 Noise Detection and Removal
 Segmentation
 Geometric measurement
 Scene analysis (Region Labelling)
14
Retinal Image Analysis using Fuzzy Logic
 One of the main aspects in Retinal Image Analysis is Edge
detection of the Blood Vessels Network in the retinal images
 We enhanced the appearance of blood vessel network in the
segmented retinal images through various edge detection
techniques.
15
Fuzzy Inference System
 Fuzzy inference is the process of formulating the mapping
from a given input to an output using fuzzy logic.
 In order to compute the output of a given FIS from the
inputs, these five steps should be done:
o Fuzzifying Inputs
o Applying Fuzzy Operators
o Applying Implication Methods
o Aggregating all outputs
o Defuzzifying
16
Proposed Fuzzy Inference System
 Mamdani FIS by taking a movable window over the image of
2x2 size.
 Inputs: Two of them, which are the gradients with respect to
x-axis and y-axis.
 Output: Edges
17
Proposed Fuzzy Inference System
 For both the fuzzy variables, the membership functions
are Gaussian which are:
 LOW: gaussmf(43,0)
 MEDIUM: gaussmf(43,127)
 HIGH: gaussmf(43,255)
18
Proposed Fuzzy Inference System
 Rules
 If (DH is LOW) and (DV is LOW) then (EDGES is LOW)
 If (DH is MEDIUM) and (DV is MEDIUM) then (EDGES is LOW)
 If (DH is HIGH) and (DV is HIGH) then (EDGES is HIGH)
 Output
19
Simulation Output
 Edge Detection through Canny Method
Canny Fuzzy Canny
20
Simulation Output
 Edge Detection through Sobel Method
Sobel Fuzzy Sobel
21
Simulation Output
 Edge Detection through Prewitt Method
Prewitt Fuzzy Prewitt
22
Simulation Output
 Edge Detection done through our project code
Project code Fuzzy Project Code
23
24

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Detection of eye disorders through retinal image analysis

  • 1. Detection of Eye Disorders Through Retinal Image Analysis Blood Vessel Segmentation, Optic Disc Segmentation and Fuzzy Logic Image Processing By Rahul Dey
  • 2. Overview of the Presentation  Common Eye Disorders Blood and Optic disk Segmentation  Literature Survey  Algorithm  Simulation Fuzzy Logic Image Processing  Introduction  Fuzzy Inference System  Implementation on Edges 2
  • 3. Common Retinal Eye Disorders 3 Fig .2 GlaucomaFig.3 Diabetic Retinopathy Source : www.fau.de • Glaucoma is associated with elevated pressure in eye which damages optic nerve • DR is a common retinal complication associated with diabetes Fig.1 Normal Eye
  • 4. Literature Survey For Optic Disk Segmentation Extraction of optic disk, fovea, and blood vessel are used for comprehensive analysis and grading of diabetic retinopathy Other symptoms which can be detected are cotton wool spot, Microaneurysms and haemorrages. Methods :  Circular hough transformation for detection optic disk  Curvlet transformation  Artificial neural network Source : www.fau.de 4
  • 5. Literature Survey For Blood Vessel Segmentation Blood vessel provides nourishment to retina while diabetes may weaken and leak blood vessel forming dot like haemorrages These leaking vessels often lead to swelling and decreased vision Blood vessels are segmented to locate optic disk and fovea 5 Fig.4 Segmented Blood vessel
  • 6. Algorithm 6 Read image & set threshold for , blood vessel segmentation and optic disk dilating window Blood vessel segmentation starts  Resize image to 576 × 576  Read green channel because green channel has the highest contrast  Performing morphological operation to highlight blood vessel 𝐶 = 𝐴 ⊕ 𝐵2 ⊖ 𝐵2 − (𝐴 ⊕ 𝐵1) ⊖ 𝐵1  𝐵1, 𝐵2 having size 1 to 6  Adaptive histogram equalization  Gaussian filtering ( 𝜎 = 2 )  Median filtering having kernel size 2 x 2  Binarization with user defined threshold
  • 7. Algorithm (contd..)  Thinning operation  Median filtering having kernel size 2 x 2  Filling and dilation  End of blood vessel segmentation  Optic disk segmentation starts  Read image  Extract red plane  Extract green plane  Read template of user defined size  Extract red plane of the template  Do normalize correlation 𝑖=1 𝑀 (𝑇 𝑖− 𝑇)(𝐼 𝑖,𝑣− 𝐼) 𝑖=1 𝑀 (𝑇𝑖 − 𝑇)2 𝑖=1 𝑀 (𝐼𝑖,𝑣− 𝐼 𝑣)2 7
  • 8. Algorithm (contd..)  If  Correlation co-efficient is > user defined threshold then extract optic disk  Dilate extracted optic disk with square window of 3 x 3 to 4 x 4  Take mean value of the dilated image as threshold  Binarize the image  Median filter of size 3 x 3 to 4 x 4  Open by taking kernel size of 4 x 4  Fill the image  Perform close operation on the image with disk shape kernel of radius 2 to 3 pixel  Show image  Canny edge detection  Else  Display error message  End of Optic disk segmentation 8
  • 10. Difference Between Proposed & Our Methodology Proposed Our • Gaussian filtering is not done • Additional Gaussian filtering done • Green channel used for optic disk localization • Red channel used for optic disk localization • Green channel used for optic disk segmentation • Red channel used for optic disk segmentation • Filtering kernel size information are missing • All filtering sizes are computed 10
  • 11. Blood Vessel Result Comparison 11
  • 13. Fuzzy Logic Image Processing(FLIP)  Fuzzy image processing is a combination of fuzzy approach to image processing.  Fuzzy image processing stages:  After the image data are transformed from gray-level plane to the membership plane (fuzzification), appropriate fuzzy techniques modify the membership values. 13
  • 14. Applications of Fuzzy Logic Image Processing  Contrast Enhancement  Edge Detection  Noise Detection and Removal  Segmentation  Geometric measurement  Scene analysis (Region Labelling) 14
  • 15. Retinal Image Analysis using Fuzzy Logic  One of the main aspects in Retinal Image Analysis is Edge detection of the Blood Vessels Network in the retinal images  We enhanced the appearance of blood vessel network in the segmented retinal images through various edge detection techniques. 15
  • 16. Fuzzy Inference System  Fuzzy inference is the process of formulating the mapping from a given input to an output using fuzzy logic.  In order to compute the output of a given FIS from the inputs, these five steps should be done: o Fuzzifying Inputs o Applying Fuzzy Operators o Applying Implication Methods o Aggregating all outputs o Defuzzifying 16
  • 17. Proposed Fuzzy Inference System  Mamdani FIS by taking a movable window over the image of 2x2 size.  Inputs: Two of them, which are the gradients with respect to x-axis and y-axis.  Output: Edges 17
  • 18. Proposed Fuzzy Inference System  For both the fuzzy variables, the membership functions are Gaussian which are:  LOW: gaussmf(43,0)  MEDIUM: gaussmf(43,127)  HIGH: gaussmf(43,255) 18
  • 19. Proposed Fuzzy Inference System  Rules  If (DH is LOW) and (DV is LOW) then (EDGES is LOW)  If (DH is MEDIUM) and (DV is MEDIUM) then (EDGES is LOW)  If (DH is HIGH) and (DV is HIGH) then (EDGES is HIGH)  Output 19
  • 20. Simulation Output  Edge Detection through Canny Method Canny Fuzzy Canny 20
  • 21. Simulation Output  Edge Detection through Sobel Method Sobel Fuzzy Sobel 21
  • 22. Simulation Output  Edge Detection through Prewitt Method Prewitt Fuzzy Prewitt 22
  • 23. Simulation Output  Edge Detection done through our project code Project code Fuzzy Project Code 23
  • 24. 24