Detection of antibiotic residues in milk
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This document discusses methods for detecting antibiotic residues in milk, including microbiological, chromatographic, and immunochemical tests. It focuses on microbioogical screening tests that use bacterial strains like B. stearothermophilus to detect residues. Rapid screening kits are described that can detect several common antibiotic classes in 6 minutes or less from bulk milk tanks. Maintaining low levels of antibiotic residues in milk is important to avoid disturbing gut flora or causing resistance in humans.
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This document discusses antibiotic use in milk production and the risks of antibiotic residues. It notes that antibiotic treatment of dairy cows requires a withdrawal period to ensure milk does not contain residues above the maximum residue limit (MRL) set by legislation. The presence of residues can pose health risks to consumers and processing issues. The document recommends good farm practices like record keeping of treatments, clear cow identification, and milk discard protocols to avoid mixing contaminated milk. It also discusses antibiotic residue testing that can be done on farm and by dairies to check for residues.
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Raw milk has to pass through rigorous examination which may include organoleptic,physical and chemical tests to assess the quality of intake milk rapidly, and decide for its acceptance or rejection. All these tests known as “platform tests” are performed on each can/tanker to assess the quality of the incoming milk before it is accepted and weighed. These tests must be easy to perform, give quick and reliable results and should not require complicated and elaborate equipment. The classification of milk on the basis of quality is usually referred to “grading of milk”.So grading of milk is done on the basis of platform tests which include organoleptic
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The document discusses the significance of bovine mastitis and methods for diagnosing mastitis. It notes that mastitis causes losses in milk production and quality, additional treatment costs, and premature culling. The most important methods for diagnosing mastitis involve examining the animal, udder, and milk through visual inspection, palpation, strip cup testing, and analyzing milk characteristics like pH, chloride levels, somatic cell count, and enzyme levels. Key tests discussed include the California Mastitis Test, Surf Field Mastitis Test, and measuring somatic cell count and N-acetyl-β-D-glucosaminidase activity.
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128
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The document discusses meat hygiene and public health. It covers topics like the importance of meat as food, meat inspection processes, livestock production's role in the national economy, meat production statistics, the chemical composition of different meats, and prevailing issues in the meat sector such as unhygienic processing. It also describes ante-mortem examination of food animals and various slaughtering methods including Islamic, Jewish, Sikh, and humane methods involving stunning. The Islamic method aims to cause acute blood loss and instant loss of consciousness to minimize pain according to religious guidelines.
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1) Internal contaminants from microbes inside the udder or blood of infected/carrier animals.
2) External contaminants introduced during production, handling and distribution from environments, unclean equipment/handlers, or contaminated water.
3) Maximum limits are defined for bacteria, coliforms, somatic cells, and drug residues in raw and pasteurized milk to ensure safety.
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Laboratory Manual Quality Control of Milk: Quality Control of Milk
Laboratory Manual Quality Control of Milk: Quality Control of Milk
Lecture 1 : Grading, Testing & Quality of raw milk
Lecture 1 : Grading, Testing & Quality of raw milk
Dr.a.k.srivastava chemical and drug residues in dairy products (ayurvet semin...
Dr.a.k.srivastava chemical and drug residues in dairy products (ayurvet semin...
Similar to Detection of antibiotic residues in milk
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This document discusses monitoring veterinary drug residues in milk and milk products in India. It summarizes several testing methods available, including microbial inhibitor tests, enzyme inhibition tests, and immune receptor tests. Microbial inhibitor tests are reliable and cost-effective, but require several hours for incubation. The document also discusses developing a novel test using bacterial spores as biosensors that can detect residues in under 3 hours and has been patented. Commercial tests based on enzyme inhibition and immune receptor principles are also briefly described.
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- 1. Detection Of Antibiotic Residues In Milk
- 2. Antibiotics Used On The Dairy •Different groups of antibiotics which are available to treat infected dairy cattle •Most common groups include: 1. beta-lactams (e.g. penicillin), 2.sulphonamides (e.g. sulfamethazine), 3.aminoglycosides (e.g. streptomycin) 4.tetracyclines (e.g. tetracycline) • These antibiotics may be used singly or at times in combination when treating dairy cattle.
- 3. Methods Use For Antibiotic Residues • Various analytical methods use to determine antibiotic residues in milk such as: 1. micro-biological, 2. chromatographic, 3. immunochemical, 4. receptor and enzyme-based tests. • Microbiological tests are commonly applied in dairy.For the detection of antimicrobial residues in milk; micro-biological screening tests are used that utilize bacterial test strains such as Bacillus stearothermophilus , Streptococcus thermophilus and Bacillus subtilis
- 4. Minimum number of antibiotic residues in milk disturbed normal flora of gut and cause resistance in a person. Maximum number of antibiotic residues in milk cause allergy and other immune disturbance. After 72hrs (mean use after given the dose of medicine 3 days)
- 5. Rapid Antibiotic Residue Testing –Milk A range of kits for rapid antibiotic residue testing in milk- giving you fast, accurate answers you need to ensure milk safety. The rugged, easy-to-read SNAP tests detect antibiotic and other residues in either bulk or individual milk samples. With millions of SNAP tests sold, IDEXX SNAP tests are known worldwide for fast, reliable residue detection.
- 6. SNAP MRL BETA-LACTAM Test Kit Detects penicillin in raw, commingled cow milk at or below the European Maximum Residue Limit (MRL). Beta-Lactams are the most widely used antibiotics in the treatment of mastitis and other infections in dairy cows.
- 7. SNAP Beta-Lactam ST Test detects beta-lactam residues in raw, commingled cow milk in just 6 minutes, without heat or incubation, so you can test milk samples directly from truck, bulk tank or refrigerator
- 8. SNAPduo Beta-Lactam ST Test It use for the tests of both beta-lactams and tetracycline. SNAP TETRACYCLINE TEST KIT SNAP AFLATOXIN TEST KIT SNAP GENTAMYCINE TEST KIT
- 9. Trisensor/Twinsensor Quick results: Trisensor test only takes 6 minutes to run It does not require any cleaning or sample preparation Can detect the 3 families of antibiotics near their respective Maximum Residue Limit (MRL) values Results can be interpreted by direct visual observation MRL-suitable readings are given by an optical reader Accurate interpretations means no subjectivity
- 10. Indicator B. stearothermophilus is widely used for detection of antibiotics in milk, because it is very sensitive to what is considered the most important group of antimicrobials for this matrix, the β-lactam antibiotics.