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CURRICULUM VITAE
Name: Edward B. Siwak Date of Birth: January 27, 1950
Place of Birth: Natrona Heights, Pennsylvania
Current Addresses:
(Work) (Home)
Baylor College of Medicine
Department of Molecular Virol. and Microbiol.
1 Baylor Plaza, Room 802D 3030 Conway St.
Houston, TX 77030 Houston, TX 77025
Phone:713.798.3009 Phone:713.669.0587
FAX:713.798.3490 Cell:832.518.0895
E-mail:esiwak@bcm.edu E-mail:edsiwak@comcast.net
EDUCATION (Transcripts available upon request)
Bachelor of Science (Biology) 1972: School of Arts and Sciences, Indiana University of
Pennsylvania. Minor in Social Sciences.
Master of Science (Epidemiology) 1982: School of Public Health, University of Hawaii at
Manoa. Master's Thesis Title: Comparative Stability of Purified Strains of Accepted and
Proposed Indicator Bacteria in Marine Waters of Oahu.
Doctor of Philosophy (Microbiology) 1990: Department of Microbiology, University of Hawaii at
Manoa. Doctoral Dissertation Title: Recovery and Characterization of Human Rotaviruses
(HRV) in Infant and Children's Clinical Stool Specimens Obtained From Two Hospitals on
Oahu.
PROFESSIONAL EXPERIENCE
Teaching - University of Hawaii (Oahu, Hawaii)
1980 -1982, spring semester: assisted in teaching the laboratory and lecture of Water Quality
Microbiology (PH/CE 636).
1984, fall semester: taught the Microbial Ecology laboratory (MICRO 485).
1985, spring semester: taught the Virology of Animal Viruses laboratory (MICRO 490).
1985, fall semester: taught the Biology of Microorganisms laboratory (MICRO 351).
1986, spring semester: taught the Virology of Animal Viruses laboratory (MICRO 490).
1987, spring semester: assisted in teaching the laboratory and lecture of Water Quality
Microbiology (PH/CE 636).
Created: 07 June 2016 Page 1 of 11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
1988, spring semester: assisted in teaching the laboratory and lecture of Water Quality
Microbiology (PH/CE 636).
1988, summer session: assisted in teaching the lecture of General Microbiology (MICRO 130)
in continuing education at Kapiolani Community College.
1988, fall semester: assisted in teaching the lecture of General Microbiology (MICRO 130) in
continuing education at the University of Hawaii.
1989, spring semester: taught the General Microbiology laboratory (MICRO 140).
1989, spring semester: assisted in teaching the lecture of General Microbiology (MICRO 130)
in continuing education at Hickam U.S. Air Force Base, Oahu, Hawaii.
1989, fall semester: assisted in teaching the lecture of General Microbiology (Micro 130).
Research - University of Hawaii (HI)
Oct 1977 - Jan 1978. Casual hire for Dr. R.S. Fujioka as an assistant researcher.
Jan 1978 - Jul 1983. Graduate research assistant for Dr. R.S. Fujioka. Projects involved in: 1)
bactericidal effect of sunlight; 2) Makakilo well survey; 3) concentration and identification of
human rotaviruses.
Aug 1983 - Jun 1984. Graduate research assistant for Dr. R.S. Fujioka. Project: development
of a reliable method to concentrate and identify human rotaviruses in sewage and natural
waters.
Aug 1986 - Sep 1986. Casual hire for Dr. R.S. Fujioka as a research assistant. Project:
development of a highly sensitive and rapid methodology for the detection of human
enteropathogenic viruses in water.
Oct 1986 - Mar 1990. Research assistant for Drs. R.S. Fujioka and P.C. Loh. Project:
development of a highly sensitive and rapid methodology for the detection of human
enteropathogenic viruses in water.
Research - Southwest Foundation for Biomedical Research (San Antonio,
Texas)
May 1990 - Jan 1993. Postdoctoral scientist in the Department of Virology and Immunology,
and the Center for AIDS Research at the Southwest Foundation for Biomedical Research, San
Antonio, Texas.
Research - Baylor College of Medicine (Houston, Texas)
Feb 1993 - Jun 1994. Research Associate in the Division of Molecular Virology at Baylor
College of Medicine, Houston, Texas.
Jul 1994 - Jan 1998. Postdoctoral Associate in the Division of Molecular Virology at Baylor
College of Medicine, Houston, Texas, 77030.
Created: 07 June 2016 Page 2/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
Feb 1998 – Dec 2001. Research Associate in the Department of Otorhinolaryngology at Baylor
College of Medicine, Houston, Texas, 77030.
Jan 2002 – Present. Assistant Professor in the Division of Molecular Virology and Microbiology
at Baylor College of Medicine, Houston, Texas, 77030.
EDITORIAL SERVICE
May 1990 through 1993: Ad hoc reviewer for Viral Immunology.
Oct 1994 through 1995: Ad hoc reviewer for Journal of Clinical Microbiology.
PUBLICATIONS
Siwak EB. Sunlight identified as the primary cidal factor for enteric indicator bacteria in marine
and fresh stream waters. Sea Grant Today 1981;11:19.
Fujioka RS, Hashimoto HH, Siwak EB and Young RHF. Effect of sunlight on survival of
indicator bacteria in seawater. Appl Environ Microbiol 1981;41:690-96.
Fujioka RS, Siwak EB and Loh PC. Replication of Human Rotavirus in Tissue Culture:
Recovery and Detection in Fecal, Sewage, and Natural Water Samples. 1984(June). Technical
Report No. 161; Water Resources Research Center. University of Hawaii, Honolulu, HI 96822.
Fujioka RS and Siwak EB. The Cidal Effect of Sunlight on Alternative Microbial Indicators of
Water Quality. pp. 495-507. In: Proceedings: Water Quality Technology Conference. Houston,
Texas; December 8-11, 1985. American Water Works Association. (WQTC-13).
Fujioka RS and Siwak EB. Bactericidal Properties of Long UV and Visible Wavelengths of
Sunlight. pp.469-486. In: Proceedings: Water Quality Technology Conference. Baltimore,
Maryland; November 15-20, 1987. American Water Works Association. (WQTC-15).
Chanh TC, Siwak EB and Hewetson JF. Anti-idiotype-based vaccines against biological toxins.
Toxicol Appl Pharmacol 1991;108:183-93.
Palmer CJ, Lee MH, Bonilla GF, Javier BJ, Siwak EB, and Tsai YL. Analysis of sewage
effluent for human immunodeficiency virus (HIV) using infectivity assay and reverse-
transcriptase polymerase chain reaction. Can J Microbiol 1995;41:809-15.
Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Single strand conformation polymorphism
study of human immunodeficiency virus type 1 cDNA and DNA in plasma, peripheral blood
mononuclear cells (PBMC) and in their virologic cultures. J Infect Dis 1995;171:1619-22.
Lauder IJ, Lin HJ, Siwak EB, and Hollinger FB. Kernal density analysis of variable and
conserved regions of the envelope proteins of human immunodeficiency virus type 1 and
associated epitopes. AIDS Res Human Retro 1996;12:91-97.
Scott WA, Brambilla D, Siwak E, Beatty C, Bremer J, Coombs RW, Farzadegan H, Fiscus SA,
Hammer SM, Hollinger FB, Khan N, Rasheed S, Reichelderfer PS, and Participating
Laboratories of the AIDS Clinical Trials Group. Evaluation of an infectivity standard for real-
Created: 07 June 2016 Page 3/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
time quality control of Human Immunodeficiency Virus Type1 quantitative micrococulture
assays. J Clin Microbiol 1996;34:2312-15.
Birdsall HH, Trial J, Lin HJ, Green DM, Sorrentino GW, Siwak EB, Rossen RD.
Transendothelial migration of lymphocytes from HIV-1 infected donors: a mechanism for
extravascular dissemination of HIV-1. J Immunol 1997 Jun 15;158(12):5968-77.
Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Selection of appropriate HIV-1 genomic
regions for single-strand conformation polymorphism analysis of the diversity, modification,
and transmission of HIV-1 quasispecies. J Acquir Immune Defic Syndr and Hum Retro
1998;18:409-16.
Lathey JL, Brambilla D, Goodenow MM, Nokta M, Rasheed S, Siwak EB, Bremer JW, DD
Huang, Yi Y, Reichelderfer PS, and Collman RG for the Macrophage Tropic Viral Kinetic Team
(MTVK), Pediatric AIDS Clinical Trials Group (PACTG), NIAID. Co-receptor usage was more
predictive than NSI/SI phenotype for HIV replication in macrophages: is NSI/SI phenotyping
sufficient? J Leukoc Biol 2000 Sep;68(3):324-30.
Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Long-term culture of human immunodeficiency
virus type 1 resulting in loss of glycosylation sites. J Med Virol 2001 Mar;63(3):197-202.
Birdsall HH, Siwak EB, Trial J, Rodriguez-Barradas M, White AC Jr, Wietgrefe S, Rossen RD.
Transendothelial migration of leukocytes carrying infectious HIV-1: an indicator of adverse
prognosis. AIDS 2002 Jan 4;16(1):5-12.
Soucie JM, Siwak EB, Hooper WC, Evatt BL, Hollinger FB; The Universal Data
Collection Project Working Group. Human parvovirus B19 in young male patients with
hemophilia A: associations with treatment product exposure and joint range-of-motion
limitation. Transfusion. 2004 Aug;44(8):1179-85.
Hongjun J, Siwak EB, Smith R, and LiWang P. Retracted: Very strong synergy between
modified RANTES and gp41 binding peptides leads to potent anti-HIV-1 activity. Antimicrobial
Agents and Chemotherapy In press. 2008
Nance CL, Siwak EB, and Shearer WT. Preclinical development of the green tea catechin,
epigallocatechin gallate, as an HIV-1 therapy. J Allergy Clin Immunol 2009;123:459-65.
Nance CL, Siwak EB, D'Souza M, Songa S, McMullen AM and Shearer WT. Inhibition of
HIV-1 infectivity across subtypes by the green tea catechin, epigallocatechin gallate,
without altered immune function. J Immunol, 2009, 182:128.6
Arora R, Bull L; Siwak EB, Arduino RC, Kimata JT. Dendritic cell-mediated HIV-1 infection of
T-cells demonstrates a direct relationship to plasma viral RNA levels. J Acquir Immune Defic
Syndr 2010 Jun 1;54;2:115-21.
Thippeshappa R, Polacino P, Kimata MTY, Siwak EB, Anderson D, Wang W, Sherwood L,
Arora R, Wen M, Zhou P, Hu S-L, and Kimata JT. A Minimal Vif Substitution Enables
Persistent Infection of Pig-tailed 1 Macaques by HIV-1. J Virol 2011;85(8):3767-79.
Created: 07 June 2016 Page 4/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
Lü JM1, Yan S, Jamaluddin S, Weakley SM, Liang Z, Siwak EB, Yao Q, Chen C. Ginkgolic
acid inhibits HIV protease activity and HIV infection in vitro. Med Sci Monit. 2012
Aug;18(8):BR293-298.
C. Marin-Muller, A. Rios, D. Anderson, E. Siwak, Q. Yao. Complete and repeatable inactivation
of HIV-1 viral particles in suspension using a photo-labeled non-nucleoside reverse
transcriptase inhibitor. Journal of Virological Methods 2013;189(1):125-28
Das, Kishore, Georgina De la Garza, Edward B. Siwak, Virginia L. Scofield, Subramanian
Dhandayuthapani. Mycoplasma genitalium promotes epithelial crossing and peripheral blood
mononuclear cell infection by HIV-1. Intl J Infect Dis 2014 23:31–38.
Rios A, Poteet EC, Siwak EB, Anderson DW, Yao QC. HIV inactivation: time for a second
look. AIDS. 2015 29(1):129-31.
Jamaluddin, Md Saha, Pei-Wen Hu, Yih Jan, Edward B. Siwak, and Andrew P. Rice. The
broad spectrum histone deacetylase inhibitors Vorinostat and Panobinostat activate latent HIV
in CD4+ T cells in part through phosphorylation of the T-loop of the CDK9 subunit of P-TEFb.
AIDS Research and Human Retroviruses. AIDS Res Hum Retroviruses. 2016 Feb;32(2):169-
73.
CONFERENCE PARTICIPATION
American Society for Microbiology, annual meeting, Honolulu branch, 1979. Title of paper:
Comparative Survival of Purified Culture of Fecal Coliform (FC), Fecal Streptococci (FS),
Salmonella Groups E and D, Pseudomonas aeruginosa, and Staphylococcus aureus in Fresh
and Marine Waters.
American Society for Microbiology, annual meeting, Honolulu branch, 1980. Title of paper:
Comparative Stability of Purified Versus Natural Sewage Populations of Indicator Bacteria
Suspended in Seawater and Exposed to Sunlight.
American Society for Microbiology, annual meeting, National Conference, Miami Beach,
Florida. 1980. Title of paper: Bactericidal Effect of Sunlight on Fecal Coliform (FC) and
Fecal Streptococci (FS) in Marine, Estuary, and Fresh Waters and Its Effect on the FC:FS
Ratio.
American Society for Microbiology, annual meeting, Honolulu branch, 1981. Title of paper:
Characterization of the Wavelength of Sunlight Responsible for the Cidal Effects Observed on
Indicator Bacteria.
American Society for Microbiology, annual meeting, Honolulu branch, 1982. Title of paper:
The Effect of Sunlight on the Enumeration of Indicator Bacteria Under Field Conditions.
American Society for Microbiology, annual meeting, Honolulu branch, 1983. Title of paper:
Variance in Sensitivity of Sewage-Borne and Purified Cultures of Fecal Coliform and Fecal
Streptococcus to Sunlight Inactivation.
American Society for Microbiology, annual meeting, National Conference, New Orleans,
Louisiana. 1983. Title of paper: Variance in Sensitivity of Sewage-Borne and Purified
Cultures of Fecal Coliform and Fecal Streptococcus to Sunlight Inactivation.
Created: 07 June 2016 Page 5/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
American Society for Microbiology, annual meeting, Honolulu branch, 1984. Title of paper:
Detection and the Cultivation of Human Rotavirus From Pediatric Cases.
American Society for Microbiology, annual meeting, Honolulu branch, 1987. Title of paper:
Isolation and Characterization of Human Rotavirus From Pediatric Patients at Two Oahu
Hospitals.
American Society for Microbiology, annual meeting, Honolulu branch, 1988. Title of paper:
Analysis of the Genomic Patterns by Electrophoresis of Human Rotavirus Recovered from
Patients in Hawaii.
American Society for Microbiology, annual meeting, National Conference, Miami Beach,
Florida. 1988. Title of paper: Detection of Rotavirus in Stools from Children Collected by Two
Hospitals in Hawaii.
American Society for Microbiology, annual meeting, National Conference, New Orleans,
Louisiana. 1989. Title of paper: Isolation and Characterization of the Infectious Hypodermal
and Hematopoietic Necrosis Virus (IHHNV) of Penaeid Shrimp.
American Society for Microbiology, annual meeting, National Conference, Anaheim, California.
1990. Title of paper: Comparative Stabilities of Rotavirus SA 11, Human Strain TH 15, and
Poliovirus 1 Under Simulated Environmental Conditions.
Federation of American Societies for Experimental Biology, annual meeting, Atlanta, Georgia,
1991. Title of paper: HTLV-I Suppresses Normal Human Killer Cell Activity In Vitro.
IX Texas Immunology Conference, Galveston, Texas, 1991. Title of paper: Complement-
Mediated Lysis of HTLV-I-Infected Cells by a Monoclonal Antibody.
34th ICAAC Meeting, Orlando, Florida, 1994. Title of paper: Evaluation of an Infectivity
Standard for Real Time Quality Control of HIV-1 Quantitative Microculture Assays.
4th International Workshop on HIV, Cells of the Macrophage Lineage, and other Reservoirs.
Title of paper: Co-receptor Usage was more Predictive than NSI/SI Phenotype for HIV Growth
in Macrophages.
Federation of American Societies for Experimental Biology, annual meeting, San Diego,
California, 2005. Title of paper: Predicting susceptibility to secondary infection in HIV-1
infected people.
CSH Retrovirus Meeting 2007. Title of Paper. Identification of miRNAs regulating cyclin T1
protein expression.
7th
Annual meeting, HIV Persistence during Therapy, Miami, Florida, December 8-11, 2015.
Title of paper: The steroid receptor coactivators are targets for reactivation of HIV latency.
AWARDS
Sea Grant Research Award, Master's Division, 1981. Paper Title: Sunlight identified as the
primary cidal factor for enteric indicator bacteria in marine and fresh stream waters
Created: 07 June 2016 Page 6/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
PROFESSIONAL SOCIETY MEMBERSHIPS
American Society for Microbiology
American Society of Molecular Pathologists
RESEARCH INTERESTS
I. Research interests and skills developed at the University of Hawaii
(Masters program): The area of research was focused on environmental
bacteriology.
1. Primary detection, isolation, propagation and characterization of enteric and non-enteric
bacterial strains from natural waters (stream and ocean), domestic wastewater and treated
wastewater effluents, and well-water in collaboration with the Board of Water Supply, City
of Honolulu and the State of Hawaii Department of Health.
2. Bacteriophage isolation from environmental sources.
3. Bacteriological media development.
4. Ames' mutagenic testing of compounds utilizing the Ames strains of Salmonella.
5. Photoinactivation of bacteria and bacteriophage in the environment/laboratory.
6. Evaluation of the Environmental Protection Agency's bacterial indicator standards.
II. Research interests and skills developed at the University of Hawaii
(Doctoral program): The area of research was focused on environmental
virology.
1. Cell culturing, and cryopreservation techniques used in virology.
2. Primary isolation and propagation of enteric viruses from environmental and clinical
samples.
3. Developed a plaque assay for detection and enumeration of human rotavirus (HRV).
4. Isolation of nucleic acid from picorna-, reo- and HRV.
5. Electrophoresis, both agarose and polyacrylamide gel (PAGE), of nucleic acids, and
visualization of nucleic acid by ethidium bromide and silver staining.
6. Spectroscopy.
7. Transmission electron microscopy (TEM).
8. Enzyme immunoassay techniques (ELISA, Western Blot analysis, Dot-immunoblot
analysis).
9. Production and purification of virus specific antibodies in laboratory animals.
Created: 07 June 2016 Page 7/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
10. Growth and maintenance of primate cell lines.
11. Cryopreservation of virus stocks and cell lines.
III. Research interests and skills developed at the Southwest Foundation
for Biomedical Research (Postgraduate studies): The area of research was
focused on retroviral immunology.
1. Isolation and propagation of human and primate peripheral blood mononuclear cells
(hPBMCs).
2. Proliferation of PBMCs and inhibition of mitogen-mediated proliferation of PBMCs.
3. Radioimmunoprecipitation assay (RIPA) of PBMC surface markers with the intention of
identifying the receptor molecule of the human retrovirus T-lymphotropic Virus Type-1
(HTLV-1).
4. Iodination and metabolic labeling of normal hPBMC.
5. Natural killer (NK) cell assays.
6. Immunofluorescent staining (Becton Dickinson FACScan).
7. Tritiated thymidine cell labeling and cell harvesting analyzed by liquid scintillation.
8. Monoclonal antibody production and hybridoma methods, including generation of human
monoclonal antibodies.
9. Protein PAGE.
10. Western Blot analysis of HTLV-I proteins by generated monoclonal antibodies.
11. Propagation of the retroviruses HIV and HTLV-I in infected cell lines.
12. The identification and characterization of virally induced suppressor factors.
IV. Research interests and skills developed at Baylor College of Medicine
(Postdoctoral studies): The area of research was focused on clinical
retrovirology (HIV-1) and PCR analysis.
1. Qualitative/quantitative coculturing of PBMC for HIV-1 infection; ELISA testing of HIV-1
culture supernatants for the presence of HIV-1 p24 antigen.
2. Processing of clinical specimens and PCR analysis for the presence of viral nucleic acid
including hepatitis C virus, HIV-1 RNA [viral], and HIV-1 DNA [proviral].
3. Coculturing of clinical specimens in MT-2 cells (MT-2 assay) for the determination of ability
of clinical HIV-1 isolates to induce syncytium formation (SI vs. NSI).
4. Culturing and cryopreservation of HIV-1 in both small (< 10 mL) and high volume (> 1L)
batches for long term storage.
5. Susceptibility testing of anti-HIV-1 compounds in vitro.
Created: 07 June 2016 Page 8/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
6. Culture, enumeration, and cryopreservation of both naive and drug resistant (foscarnet)
strains of human cytomegalovirus (HCMV).
7. Development of assay and analysis of RNA-containing virus quasispecies (HIV-1 and
hepatitis C virus) by the use of single-stranded conformational polymorphism (SSCP).
8. Participation with various other AIDS Clinical Trials Group laboratories in pilot projects
including: i) the neutralization working group using an in vitro HIV-1 model system and
immune human sera, ii) the real time culture quality assurance working group (published),
iii) the Macrophage Tropism-Viral Kinetics working group, to define monocytotropic HIV-1
strains, and iv) the HIV-1 SI/NSI (syncytium inducing/nonsyncytium inducing) study from
the WITS (Women to Infant Transmission Study) group.
9. Maintained a CAP Laboratory under the direction of Dr. F. Blaine Hollinger through the
Eugene B. Casey Laboratory for the testing of patient specimens for HIV-1, HBV and HCV
by PCR, and testing of patient specimens for hepatitis B and C by EIA tests [Annual
Inspection by: The College of American Pathologists].
10. Collaboration with the Pediatric AIDS group led by Dr. William Shearer at the Texas
Children's Hospital on a long term survivor study of HIV-1 infected children.
11. Collaboration with Dr. Catherine L. Troisi, Division of Molecular Virology, Baylor College of
Medicine on i) the development of a dual HIV-1-HCV immunoassay for the identification of
both pathogens in the blood banking industry, and ii) detection of HCV immune complexes
in patients' serum.
12. Collaboration with Dr. Frank Gelder, LSU Medical Center, Shreveport, Louisiana, on a
clinical trial for a therapeutic protocol in adult AIDS patients.
13. Collaboration with Dr. H.J. Lin, Division of Molecular Virology, Baylor College of Medicine,
on i) quasispecies fluxes in HIV-1 infections, and ii) genetic variability of HIV-1.
14. Participation in contract writing with the culmination of multiyear awards of contracts from
the NIH (for maintaining a Reagent Assurance Laboratory for the AIDS Clinical Trials
Group); and from CDC (for conducting a hepatitis monitoring program utilizing PCR and
EIA techniques).
V. Research interests and skills developed at the Veteran’s Affairs Medical
Center (Research Associate): The area of research was focused on clinical
retrovirology (HIV-1) and flow cytometry.
1. Flow cytometry.
2. Molecular virology techniques including: i. the transformation of E. coli with genetically
engineered plasmids containing HIV-1 constructs; ii. high-volume plasmid production,
isolation, and characterization by restriction enzymes; iii. transfection of 293 mammalian
cells for production of infectious HIV-1, and iv. in situ hybridization for HIV-1 viral RNA and
cellular proviral DNA.
3. Culturing and maintenance of human umbilical vein endothelial cells (hUVEC), and
primary human astrocytes, both used in transendothelial migration of HIV-1 infected cells.
Created: 07 June 2016 Page 9/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
4. Culture, enumeration, and cryopreservation of human cytomegalovirus (hCMV), and the
study of the effect of HCMV-infected endothelial cells upon the transendothelial migration
of HIV-1 infected human peripheral blood mononuclear cells (hPBMC).
5. Continuing Collaboration with Dr. H.J. Lin, Division of Molecular Virology, Baylor College
of Medicine, on i) quasispecies fluxes in HIV-1 infections, and ii) genetic variability of HIV-
1.
VI. Research interests and duties at Baylor College of Medicine (Assistant
Professor):
1. Hepatitis epidemiology in U.S. hemophilia patients.
2. Novel hepatitis agents: TT virus and SEN virus.
3. Newly emerging blood-borne pathogens.
4. Parvovirus B19 infection in U.S. hemophilia patients.
5. Ongoing Collaboration with Dr. Christine Nance (TCH) on research examining the Green
Tea extract EGCG for its role in blocking HIV-1 infections.
6. Regulatory and Quality Control/Assurance experience as a member of a CAP Certified
laboratory. Manager of a laboratory doing processing work for the HIV Vaccine Trials
Network (HVTN) project out of Fred Hutchinson Cancer Research Center, 1100 Fairview
Ave. N., E4-200, Seattle, WA 98109. Both positions required federal auditing.
7. Ongoing Collaboration with Dr. Bing-Yan Wang (UT Dental School) in the investigation of
an oral rinse in inactivating HIV-1.
8. Experimental usage of anti-HIV-1 compounds on the suppression of HIV-1 expression in
susceptible cells ex vivo.
9. Investigation of various compounds active in cell signaling and their ability to reactivate
HIV-1 in an in vitro latency model system; ongoing collaboration with Drs. Qin Feng and
Bryan Nikolai (Cell Biology).
10. 2002-2004: Member of the Baylor Infectious Agents and Hazardous Chemicals Committee
(IAHCC).
11. 2002: Assistant Director of the Casey Diagnostic Laboratory, Department of Molecular
Virology and Microbiology, at Baylor College of Medicine.
12. 2003 - present: Laboratory Manager of the Department of Molecular Virology and
Microbiology 8th
Floor BSL-2/3 Facility.
13. 2004 - present: Associate Director of the Virology Core Laboratory of the Center for AIDS
Research (CFAR) at Baylor College of Medicine.
14. 2011 – 2014: Maintained a Federally Monitored Laboratory for the HIV-1 Vaccine Trials
Network (HVTN) [Annual Inspection by: NIAID HIV and other Infectious Diseases Clinical
Research Support Services (HIV CRSS)].
Created: 07 June 2016 Page 10/11 Modified: 16 June 2016
Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas
15. 2015 – present. HIV Latency Working Group in the Department of Molecular Virology and
Microbiology.
Created: 07 June 2016 Page 11/11 Modified: 16 June 2016

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CV_SiwakE-06072016

  • 1. CURRICULUM VITAE Name: Edward B. Siwak Date of Birth: January 27, 1950 Place of Birth: Natrona Heights, Pennsylvania Current Addresses: (Work) (Home) Baylor College of Medicine Department of Molecular Virol. and Microbiol. 1 Baylor Plaza, Room 802D 3030 Conway St. Houston, TX 77030 Houston, TX 77025 Phone:713.798.3009 Phone:713.669.0587 FAX:713.798.3490 Cell:832.518.0895 E-mail:esiwak@bcm.edu E-mail:edsiwak@comcast.net EDUCATION (Transcripts available upon request) Bachelor of Science (Biology) 1972: School of Arts and Sciences, Indiana University of Pennsylvania. Minor in Social Sciences. Master of Science (Epidemiology) 1982: School of Public Health, University of Hawaii at Manoa. Master's Thesis Title: Comparative Stability of Purified Strains of Accepted and Proposed Indicator Bacteria in Marine Waters of Oahu. Doctor of Philosophy (Microbiology) 1990: Department of Microbiology, University of Hawaii at Manoa. Doctoral Dissertation Title: Recovery and Characterization of Human Rotaviruses (HRV) in Infant and Children's Clinical Stool Specimens Obtained From Two Hospitals on Oahu. PROFESSIONAL EXPERIENCE Teaching - University of Hawaii (Oahu, Hawaii) 1980 -1982, spring semester: assisted in teaching the laboratory and lecture of Water Quality Microbiology (PH/CE 636). 1984, fall semester: taught the Microbial Ecology laboratory (MICRO 485). 1985, spring semester: taught the Virology of Animal Viruses laboratory (MICRO 490). 1985, fall semester: taught the Biology of Microorganisms laboratory (MICRO 351). 1986, spring semester: taught the Virology of Animal Viruses laboratory (MICRO 490). 1987, spring semester: assisted in teaching the laboratory and lecture of Water Quality Microbiology (PH/CE 636). Created: 07 June 2016 Page 1 of 11 Modified: 16 June 2016
  • 2. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas 1988, spring semester: assisted in teaching the laboratory and lecture of Water Quality Microbiology (PH/CE 636). 1988, summer session: assisted in teaching the lecture of General Microbiology (MICRO 130) in continuing education at Kapiolani Community College. 1988, fall semester: assisted in teaching the lecture of General Microbiology (MICRO 130) in continuing education at the University of Hawaii. 1989, spring semester: taught the General Microbiology laboratory (MICRO 140). 1989, spring semester: assisted in teaching the lecture of General Microbiology (MICRO 130) in continuing education at Hickam U.S. Air Force Base, Oahu, Hawaii. 1989, fall semester: assisted in teaching the lecture of General Microbiology (Micro 130). Research - University of Hawaii (HI) Oct 1977 - Jan 1978. Casual hire for Dr. R.S. Fujioka as an assistant researcher. Jan 1978 - Jul 1983. Graduate research assistant for Dr. R.S. Fujioka. Projects involved in: 1) bactericidal effect of sunlight; 2) Makakilo well survey; 3) concentration and identification of human rotaviruses. Aug 1983 - Jun 1984. Graduate research assistant for Dr. R.S. Fujioka. Project: development of a reliable method to concentrate and identify human rotaviruses in sewage and natural waters. Aug 1986 - Sep 1986. Casual hire for Dr. R.S. Fujioka as a research assistant. Project: development of a highly sensitive and rapid methodology for the detection of human enteropathogenic viruses in water. Oct 1986 - Mar 1990. Research assistant for Drs. R.S. Fujioka and P.C. Loh. Project: development of a highly sensitive and rapid methodology for the detection of human enteropathogenic viruses in water. Research - Southwest Foundation for Biomedical Research (San Antonio, Texas) May 1990 - Jan 1993. Postdoctoral scientist in the Department of Virology and Immunology, and the Center for AIDS Research at the Southwest Foundation for Biomedical Research, San Antonio, Texas. Research - Baylor College of Medicine (Houston, Texas) Feb 1993 - Jun 1994. Research Associate in the Division of Molecular Virology at Baylor College of Medicine, Houston, Texas. Jul 1994 - Jan 1998. Postdoctoral Associate in the Division of Molecular Virology at Baylor College of Medicine, Houston, Texas, 77030. Created: 07 June 2016 Page 2/11 Modified: 16 June 2016
  • 3. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas Feb 1998 – Dec 2001. Research Associate in the Department of Otorhinolaryngology at Baylor College of Medicine, Houston, Texas, 77030. Jan 2002 – Present. Assistant Professor in the Division of Molecular Virology and Microbiology at Baylor College of Medicine, Houston, Texas, 77030. EDITORIAL SERVICE May 1990 through 1993: Ad hoc reviewer for Viral Immunology. Oct 1994 through 1995: Ad hoc reviewer for Journal of Clinical Microbiology. PUBLICATIONS Siwak EB. Sunlight identified as the primary cidal factor for enteric indicator bacteria in marine and fresh stream waters. Sea Grant Today 1981;11:19. Fujioka RS, Hashimoto HH, Siwak EB and Young RHF. Effect of sunlight on survival of indicator bacteria in seawater. Appl Environ Microbiol 1981;41:690-96. Fujioka RS, Siwak EB and Loh PC. Replication of Human Rotavirus in Tissue Culture: Recovery and Detection in Fecal, Sewage, and Natural Water Samples. 1984(June). Technical Report No. 161; Water Resources Research Center. University of Hawaii, Honolulu, HI 96822. Fujioka RS and Siwak EB. The Cidal Effect of Sunlight on Alternative Microbial Indicators of Water Quality. pp. 495-507. In: Proceedings: Water Quality Technology Conference. Houston, Texas; December 8-11, 1985. American Water Works Association. (WQTC-13). Fujioka RS and Siwak EB. Bactericidal Properties of Long UV and Visible Wavelengths of Sunlight. pp.469-486. In: Proceedings: Water Quality Technology Conference. Baltimore, Maryland; November 15-20, 1987. American Water Works Association. (WQTC-15). Chanh TC, Siwak EB and Hewetson JF. Anti-idiotype-based vaccines against biological toxins. Toxicol Appl Pharmacol 1991;108:183-93. Palmer CJ, Lee MH, Bonilla GF, Javier BJ, Siwak EB, and Tsai YL. Analysis of sewage effluent for human immunodeficiency virus (HIV) using infectivity assay and reverse- transcriptase polymerase chain reaction. Can J Microbiol 1995;41:809-15. Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Single strand conformation polymorphism study of human immunodeficiency virus type 1 cDNA and DNA in plasma, peripheral blood mononuclear cells (PBMC) and in their virologic cultures. J Infect Dis 1995;171:1619-22. Lauder IJ, Lin HJ, Siwak EB, and Hollinger FB. Kernal density analysis of variable and conserved regions of the envelope proteins of human immunodeficiency virus type 1 and associated epitopes. AIDS Res Human Retro 1996;12:91-97. Scott WA, Brambilla D, Siwak E, Beatty C, Bremer J, Coombs RW, Farzadegan H, Fiscus SA, Hammer SM, Hollinger FB, Khan N, Rasheed S, Reichelderfer PS, and Participating Laboratories of the AIDS Clinical Trials Group. Evaluation of an infectivity standard for real- Created: 07 June 2016 Page 3/11 Modified: 16 June 2016
  • 4. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas time quality control of Human Immunodeficiency Virus Type1 quantitative micrococulture assays. J Clin Microbiol 1996;34:2312-15. Birdsall HH, Trial J, Lin HJ, Green DM, Sorrentino GW, Siwak EB, Rossen RD. Transendothelial migration of lymphocytes from HIV-1 infected donors: a mechanism for extravascular dissemination of HIV-1. J Immunol 1997 Jun 15;158(12):5968-77. Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Selection of appropriate HIV-1 genomic regions for single-strand conformation polymorphism analysis of the diversity, modification, and transmission of HIV-1 quasispecies. J Acquir Immune Defic Syndr and Hum Retro 1998;18:409-16. Lathey JL, Brambilla D, Goodenow MM, Nokta M, Rasheed S, Siwak EB, Bremer JW, DD Huang, Yi Y, Reichelderfer PS, and Collman RG for the Macrophage Tropic Viral Kinetic Team (MTVK), Pediatric AIDS Clinical Trials Group (PACTG), NIAID. Co-receptor usage was more predictive than NSI/SI phenotype for HIV replication in macrophages: is NSI/SI phenotyping sufficient? J Leukoc Biol 2000 Sep;68(3):324-30. Lin HJ, Siwak EB, Lauder IJ, and Hollinger FB. Long-term culture of human immunodeficiency virus type 1 resulting in loss of glycosylation sites. J Med Virol 2001 Mar;63(3):197-202. Birdsall HH, Siwak EB, Trial J, Rodriguez-Barradas M, White AC Jr, Wietgrefe S, Rossen RD. Transendothelial migration of leukocytes carrying infectious HIV-1: an indicator of adverse prognosis. AIDS 2002 Jan 4;16(1):5-12. Soucie JM, Siwak EB, Hooper WC, Evatt BL, Hollinger FB; The Universal Data Collection Project Working Group. Human parvovirus B19 in young male patients with hemophilia A: associations with treatment product exposure and joint range-of-motion limitation. Transfusion. 2004 Aug;44(8):1179-85. Hongjun J, Siwak EB, Smith R, and LiWang P. Retracted: Very strong synergy between modified RANTES and gp41 binding peptides leads to potent anti-HIV-1 activity. Antimicrobial Agents and Chemotherapy In press. 2008 Nance CL, Siwak EB, and Shearer WT. Preclinical development of the green tea catechin, epigallocatechin gallate, as an HIV-1 therapy. J Allergy Clin Immunol 2009;123:459-65. Nance CL, Siwak EB, D'Souza M, Songa S, McMullen AM and Shearer WT. Inhibition of HIV-1 infectivity across subtypes by the green tea catechin, epigallocatechin gallate, without altered immune function. J Immunol, 2009, 182:128.6 Arora R, Bull L; Siwak EB, Arduino RC, Kimata JT. Dendritic cell-mediated HIV-1 infection of T-cells demonstrates a direct relationship to plasma viral RNA levels. J Acquir Immune Defic Syndr 2010 Jun 1;54;2:115-21. Thippeshappa R, Polacino P, Kimata MTY, Siwak EB, Anderson D, Wang W, Sherwood L, Arora R, Wen M, Zhou P, Hu S-L, and Kimata JT. A Minimal Vif Substitution Enables Persistent Infection of Pig-tailed 1 Macaques by HIV-1. J Virol 2011;85(8):3767-79. Created: 07 June 2016 Page 4/11 Modified: 16 June 2016
  • 5. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas Lü JM1, Yan S, Jamaluddin S, Weakley SM, Liang Z, Siwak EB, Yao Q, Chen C. Ginkgolic acid inhibits HIV protease activity and HIV infection in vitro. Med Sci Monit. 2012 Aug;18(8):BR293-298. C. Marin-Muller, A. Rios, D. Anderson, E. Siwak, Q. Yao. Complete and repeatable inactivation of HIV-1 viral particles in suspension using a photo-labeled non-nucleoside reverse transcriptase inhibitor. Journal of Virological Methods 2013;189(1):125-28 Das, Kishore, Georgina De la Garza, Edward B. Siwak, Virginia L. Scofield, Subramanian Dhandayuthapani. Mycoplasma genitalium promotes epithelial crossing and peripheral blood mononuclear cell infection by HIV-1. Intl J Infect Dis 2014 23:31–38. Rios A, Poteet EC, Siwak EB, Anderson DW, Yao QC. HIV inactivation: time for a second look. AIDS. 2015 29(1):129-31. Jamaluddin, Md Saha, Pei-Wen Hu, Yih Jan, Edward B. Siwak, and Andrew P. Rice. The broad spectrum histone deacetylase inhibitors Vorinostat and Panobinostat activate latent HIV in CD4+ T cells in part through phosphorylation of the T-loop of the CDK9 subunit of P-TEFb. AIDS Research and Human Retroviruses. AIDS Res Hum Retroviruses. 2016 Feb;32(2):169- 73. CONFERENCE PARTICIPATION American Society for Microbiology, annual meeting, Honolulu branch, 1979. Title of paper: Comparative Survival of Purified Culture of Fecal Coliform (FC), Fecal Streptococci (FS), Salmonella Groups E and D, Pseudomonas aeruginosa, and Staphylococcus aureus in Fresh and Marine Waters. American Society for Microbiology, annual meeting, Honolulu branch, 1980. Title of paper: Comparative Stability of Purified Versus Natural Sewage Populations of Indicator Bacteria Suspended in Seawater and Exposed to Sunlight. American Society for Microbiology, annual meeting, National Conference, Miami Beach, Florida. 1980. Title of paper: Bactericidal Effect of Sunlight on Fecal Coliform (FC) and Fecal Streptococci (FS) in Marine, Estuary, and Fresh Waters and Its Effect on the FC:FS Ratio. American Society for Microbiology, annual meeting, Honolulu branch, 1981. Title of paper: Characterization of the Wavelength of Sunlight Responsible for the Cidal Effects Observed on Indicator Bacteria. American Society for Microbiology, annual meeting, Honolulu branch, 1982. Title of paper: The Effect of Sunlight on the Enumeration of Indicator Bacteria Under Field Conditions. American Society for Microbiology, annual meeting, Honolulu branch, 1983. Title of paper: Variance in Sensitivity of Sewage-Borne and Purified Cultures of Fecal Coliform and Fecal Streptococcus to Sunlight Inactivation. American Society for Microbiology, annual meeting, National Conference, New Orleans, Louisiana. 1983. Title of paper: Variance in Sensitivity of Sewage-Borne and Purified Cultures of Fecal Coliform and Fecal Streptococcus to Sunlight Inactivation. Created: 07 June 2016 Page 5/11 Modified: 16 June 2016
  • 6. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas American Society for Microbiology, annual meeting, Honolulu branch, 1984. Title of paper: Detection and the Cultivation of Human Rotavirus From Pediatric Cases. American Society for Microbiology, annual meeting, Honolulu branch, 1987. Title of paper: Isolation and Characterization of Human Rotavirus From Pediatric Patients at Two Oahu Hospitals. American Society for Microbiology, annual meeting, Honolulu branch, 1988. Title of paper: Analysis of the Genomic Patterns by Electrophoresis of Human Rotavirus Recovered from Patients in Hawaii. American Society for Microbiology, annual meeting, National Conference, Miami Beach, Florida. 1988. Title of paper: Detection of Rotavirus in Stools from Children Collected by Two Hospitals in Hawaii. American Society for Microbiology, annual meeting, National Conference, New Orleans, Louisiana. 1989. Title of paper: Isolation and Characterization of the Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) of Penaeid Shrimp. American Society for Microbiology, annual meeting, National Conference, Anaheim, California. 1990. Title of paper: Comparative Stabilities of Rotavirus SA 11, Human Strain TH 15, and Poliovirus 1 Under Simulated Environmental Conditions. Federation of American Societies for Experimental Biology, annual meeting, Atlanta, Georgia, 1991. Title of paper: HTLV-I Suppresses Normal Human Killer Cell Activity In Vitro. IX Texas Immunology Conference, Galveston, Texas, 1991. Title of paper: Complement- Mediated Lysis of HTLV-I-Infected Cells by a Monoclonal Antibody. 34th ICAAC Meeting, Orlando, Florida, 1994. Title of paper: Evaluation of an Infectivity Standard for Real Time Quality Control of HIV-1 Quantitative Microculture Assays. 4th International Workshop on HIV, Cells of the Macrophage Lineage, and other Reservoirs. Title of paper: Co-receptor Usage was more Predictive than NSI/SI Phenotype for HIV Growth in Macrophages. Federation of American Societies for Experimental Biology, annual meeting, San Diego, California, 2005. Title of paper: Predicting susceptibility to secondary infection in HIV-1 infected people. CSH Retrovirus Meeting 2007. Title of Paper. Identification of miRNAs regulating cyclin T1 protein expression. 7th Annual meeting, HIV Persistence during Therapy, Miami, Florida, December 8-11, 2015. Title of paper: The steroid receptor coactivators are targets for reactivation of HIV latency. AWARDS Sea Grant Research Award, Master's Division, 1981. Paper Title: Sunlight identified as the primary cidal factor for enteric indicator bacteria in marine and fresh stream waters Created: 07 June 2016 Page 6/11 Modified: 16 June 2016
  • 7. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas PROFESSIONAL SOCIETY MEMBERSHIPS American Society for Microbiology American Society of Molecular Pathologists RESEARCH INTERESTS I. Research interests and skills developed at the University of Hawaii (Masters program): The area of research was focused on environmental bacteriology. 1. Primary detection, isolation, propagation and characterization of enteric and non-enteric bacterial strains from natural waters (stream and ocean), domestic wastewater and treated wastewater effluents, and well-water in collaboration with the Board of Water Supply, City of Honolulu and the State of Hawaii Department of Health. 2. Bacteriophage isolation from environmental sources. 3. Bacteriological media development. 4. Ames' mutagenic testing of compounds utilizing the Ames strains of Salmonella. 5. Photoinactivation of bacteria and bacteriophage in the environment/laboratory. 6. Evaluation of the Environmental Protection Agency's bacterial indicator standards. II. Research interests and skills developed at the University of Hawaii (Doctoral program): The area of research was focused on environmental virology. 1. Cell culturing, and cryopreservation techniques used in virology. 2. Primary isolation and propagation of enteric viruses from environmental and clinical samples. 3. Developed a plaque assay for detection and enumeration of human rotavirus (HRV). 4. Isolation of nucleic acid from picorna-, reo- and HRV. 5. Electrophoresis, both agarose and polyacrylamide gel (PAGE), of nucleic acids, and visualization of nucleic acid by ethidium bromide and silver staining. 6. Spectroscopy. 7. Transmission electron microscopy (TEM). 8. Enzyme immunoassay techniques (ELISA, Western Blot analysis, Dot-immunoblot analysis). 9. Production and purification of virus specific antibodies in laboratory animals. Created: 07 June 2016 Page 7/11 Modified: 16 June 2016
  • 8. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas 10. Growth and maintenance of primate cell lines. 11. Cryopreservation of virus stocks and cell lines. III. Research interests and skills developed at the Southwest Foundation for Biomedical Research (Postgraduate studies): The area of research was focused on retroviral immunology. 1. Isolation and propagation of human and primate peripheral blood mononuclear cells (hPBMCs). 2. Proliferation of PBMCs and inhibition of mitogen-mediated proliferation of PBMCs. 3. Radioimmunoprecipitation assay (RIPA) of PBMC surface markers with the intention of identifying the receptor molecule of the human retrovirus T-lymphotropic Virus Type-1 (HTLV-1). 4. Iodination and metabolic labeling of normal hPBMC. 5. Natural killer (NK) cell assays. 6. Immunofluorescent staining (Becton Dickinson FACScan). 7. Tritiated thymidine cell labeling and cell harvesting analyzed by liquid scintillation. 8. Monoclonal antibody production and hybridoma methods, including generation of human monoclonal antibodies. 9. Protein PAGE. 10. Western Blot analysis of HTLV-I proteins by generated monoclonal antibodies. 11. Propagation of the retroviruses HIV and HTLV-I in infected cell lines. 12. The identification and characterization of virally induced suppressor factors. IV. Research interests and skills developed at Baylor College of Medicine (Postdoctoral studies): The area of research was focused on clinical retrovirology (HIV-1) and PCR analysis. 1. Qualitative/quantitative coculturing of PBMC for HIV-1 infection; ELISA testing of HIV-1 culture supernatants for the presence of HIV-1 p24 antigen. 2. Processing of clinical specimens and PCR analysis for the presence of viral nucleic acid including hepatitis C virus, HIV-1 RNA [viral], and HIV-1 DNA [proviral]. 3. Coculturing of clinical specimens in MT-2 cells (MT-2 assay) for the determination of ability of clinical HIV-1 isolates to induce syncytium formation (SI vs. NSI). 4. Culturing and cryopreservation of HIV-1 in both small (< 10 mL) and high volume (> 1L) batches for long term storage. 5. Susceptibility testing of anti-HIV-1 compounds in vitro. Created: 07 June 2016 Page 8/11 Modified: 16 June 2016
  • 9. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas 6. Culture, enumeration, and cryopreservation of both naive and drug resistant (foscarnet) strains of human cytomegalovirus (HCMV). 7. Development of assay and analysis of RNA-containing virus quasispecies (HIV-1 and hepatitis C virus) by the use of single-stranded conformational polymorphism (SSCP). 8. Participation with various other AIDS Clinical Trials Group laboratories in pilot projects including: i) the neutralization working group using an in vitro HIV-1 model system and immune human sera, ii) the real time culture quality assurance working group (published), iii) the Macrophage Tropism-Viral Kinetics working group, to define monocytotropic HIV-1 strains, and iv) the HIV-1 SI/NSI (syncytium inducing/nonsyncytium inducing) study from the WITS (Women to Infant Transmission Study) group. 9. Maintained a CAP Laboratory under the direction of Dr. F. Blaine Hollinger through the Eugene B. Casey Laboratory for the testing of patient specimens for HIV-1, HBV and HCV by PCR, and testing of patient specimens for hepatitis B and C by EIA tests [Annual Inspection by: The College of American Pathologists]. 10. Collaboration with the Pediatric AIDS group led by Dr. William Shearer at the Texas Children's Hospital on a long term survivor study of HIV-1 infected children. 11. Collaboration with Dr. Catherine L. Troisi, Division of Molecular Virology, Baylor College of Medicine on i) the development of a dual HIV-1-HCV immunoassay for the identification of both pathogens in the blood banking industry, and ii) detection of HCV immune complexes in patients' serum. 12. Collaboration with Dr. Frank Gelder, LSU Medical Center, Shreveport, Louisiana, on a clinical trial for a therapeutic protocol in adult AIDS patients. 13. Collaboration with Dr. H.J. Lin, Division of Molecular Virology, Baylor College of Medicine, on i) quasispecies fluxes in HIV-1 infections, and ii) genetic variability of HIV-1. 14. Participation in contract writing with the culmination of multiyear awards of contracts from the NIH (for maintaining a Reagent Assurance Laboratory for the AIDS Clinical Trials Group); and from CDC (for conducting a hepatitis monitoring program utilizing PCR and EIA techniques). V. Research interests and skills developed at the Veteran’s Affairs Medical Center (Research Associate): The area of research was focused on clinical retrovirology (HIV-1) and flow cytometry. 1. Flow cytometry. 2. Molecular virology techniques including: i. the transformation of E. coli with genetically engineered plasmids containing HIV-1 constructs; ii. high-volume plasmid production, isolation, and characterization by restriction enzymes; iii. transfection of 293 mammalian cells for production of infectious HIV-1, and iv. in situ hybridization for HIV-1 viral RNA and cellular proviral DNA. 3. Culturing and maintenance of human umbilical vein endothelial cells (hUVEC), and primary human astrocytes, both used in transendothelial migration of HIV-1 infected cells. Created: 07 June 2016 Page 9/11 Modified: 16 June 2016
  • 10. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas 4. Culture, enumeration, and cryopreservation of human cytomegalovirus (hCMV), and the study of the effect of HCMV-infected endothelial cells upon the transendothelial migration of HIV-1 infected human peripheral blood mononuclear cells (hPBMC). 5. Continuing Collaboration with Dr. H.J. Lin, Division of Molecular Virology, Baylor College of Medicine, on i) quasispecies fluxes in HIV-1 infections, and ii) genetic variability of HIV- 1. VI. Research interests and duties at Baylor College of Medicine (Assistant Professor): 1. Hepatitis epidemiology in U.S. hemophilia patients. 2. Novel hepatitis agents: TT virus and SEN virus. 3. Newly emerging blood-borne pathogens. 4. Parvovirus B19 infection in U.S. hemophilia patients. 5. Ongoing Collaboration with Dr. Christine Nance (TCH) on research examining the Green Tea extract EGCG for its role in blocking HIV-1 infections. 6. Regulatory and Quality Control/Assurance experience as a member of a CAP Certified laboratory. Manager of a laboratory doing processing work for the HIV Vaccine Trials Network (HVTN) project out of Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., E4-200, Seattle, WA 98109. Both positions required federal auditing. 7. Ongoing Collaboration with Dr. Bing-Yan Wang (UT Dental School) in the investigation of an oral rinse in inactivating HIV-1. 8. Experimental usage of anti-HIV-1 compounds on the suppression of HIV-1 expression in susceptible cells ex vivo. 9. Investigation of various compounds active in cell signaling and their ability to reactivate HIV-1 in an in vitro latency model system; ongoing collaboration with Drs. Qin Feng and Bryan Nikolai (Cell Biology). 10. 2002-2004: Member of the Baylor Infectious Agents and Hazardous Chemicals Committee (IAHCC). 11. 2002: Assistant Director of the Casey Diagnostic Laboratory, Department of Molecular Virology and Microbiology, at Baylor College of Medicine. 12. 2003 - present: Laboratory Manager of the Department of Molecular Virology and Microbiology 8th Floor BSL-2/3 Facility. 13. 2004 - present: Associate Director of the Virology Core Laboratory of the Center for AIDS Research (CFAR) at Baylor College of Medicine. 14. 2011 – 2014: Maintained a Federally Monitored Laboratory for the HIV-1 Vaccine Trials Network (HVTN) [Annual Inspection by: NIAID HIV and other Infectious Diseases Clinical Research Support Services (HIV CRSS)]. Created: 07 June 2016 Page 10/11 Modified: 16 June 2016
  • 11. Edward B. Siwak, Ph.D. Baylor College of Medicine, Houston, Texas 15. 2015 – present. HIV Latency Working Group in the Department of Molecular Virology and Microbiology. Created: 07 June 2016 Page 11/11 Modified: 16 June 2016